Second Generation of Catfish Genetic Linkage Map and Integration of Catfish Genetic Linkage and Physical Maps using BAC-Anchored Microsatellites by Parichart Ninwichian A dissertation submitted to the Graduate Faculty of Auburn University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy Auburn, Alabama August 4, 2012 Keywords: Map integration; channel catfish; genetic linkage map; BES; physical map Copyright 2012 by Parichart Ninwichian Approved by Zhanjiang Liu, Chair, Alumni Professor of Fisheries and Allied Aquacultures Eric Peatman, Assistant Professor of Fisheries and Allied Aquacultures Jeffery Terhune, Associate Professor of Fisheries and Allied Aquacultures Huseyin Kucuktas, Research Fellow IV of Fisheries and Allied Aquacultures Nannan Liu, Professor of Entomology and Plant Pathology ii Abstract Integration of physical and linkage maps is a critical step in building necessary genome resources for fine mapping of QTL, comparative genomics, and accurate and efficient whole genome assembly. Previously, we have established both linkage maps and a physical map for channel catfish, Ictalurus punctatus, the dominant aquaculture species in the U.S. Here we added 2,030 BAC-end sequence-derived microsatellites from 1,481 physical map contigs to a high-density linkage map also containing singleton BES, EST, and anonymous microsatellite markers as well as SNPs. Average marker density across the 29 linkage groups reached 1.4 cM/marker. The increased marker density highlighted variations in recombination rates within and among catfish chromosomes. Several hundred physical map contigs were anchored to the linkage group with two or more BES markers, providing a reasonably accurate basis for determination of ratios of physical to genetic map distances. These ratios ranged from 4 kb/cM to 4,830 kb/cM with an average of 455 kb/cM. The work effectively anchored close to 50% of the catfish BAC physical map contigs (~51% of the genome) to genetic linkage groups enabling future comparative studies with teleost model species as well as providing a framework for ordering and assembly of whole genome scaffolds. iii Acknowledgements I would like to express my deep and sincere gratitude to my major professor, Dr. Zhanjiang (John) Liu, for his guidance throughout my degree program. I also would like to express my gratitude to all of my committee members and my outside reader for their time and directions of my dissertation work. I would like to extend my appreciation to many people who have taught and helped me during my graduate education including Benjaporn Somridhivej, , Atra Chaimongkol, Puttitorn Saengrungruang, Ping Li, Hong Liu, Ludmilla Kaltenboeck, Yanliang Jiang, Shaolin Wang, Jason Abernathy, Shikai Liu, Jianguo Lu, Zhenxia Sha, Wenqi Wang, Fei Chen, Yoona Lee and Lilian Wong. Finally, I would like to thank my parents and all my family members for their love, understanding and support. iv Table of contents Abstract ......................................................................................................................................... ii Acknowledgments.............................................................................................................. ..........iii List of Tables ..................................................................... ...........................................................v List of Figures....................................................................................................................... . ......vi Introduction. ............................................................................................................................ ......1 Literature Review................................................................................................................... .......3 Objectives .................................................................................................................................. 19 Materials and Methods ............................................................................................................... 20 Results ....................................................................................................................................... 25 Discussion ................................................................................................................................. 30 Conclusions ................................................................................................................................ 35 Literature Cited ....................................................................................................................... 155 v List of Tables Table 1. Development of physical map contig-specific microsatellites from BAC-end sequences ...................................................................................................... 36 Table 2. A summary of markers used for construction of genetic linkage map ......................... 37 Table 3. Characteristics of marker distribution among 29 linkage groups ................................. 38 Table 4. Characteristics of the catfish genetic map with a total of 2,557 markers at 1,836 unique map positions among the 29 linkage groups ....................................... 39 Table 5. Assessment of integration of the catfish physical and linkage maps ............................ 40 Supplemental Table 1. Detailed information regarding contig identities, estimated physical contig sizes, corresponding linkage groups, and genetic map positions ................................................................................................... 72 Supplemental Table 2. Ratios of physical to genetic distance across all 29 LGs. Ratios were estimated based on the difference of the physical distances (Kb) and genetic distances (cM) between multiple BES markers mapped from the same contigs ............................................................. 145 vi List of Figures Figure 1. An example linkage group (LG19) from the genetic linkage map of channel catfish. Genetic map distance is given in centimorgans (Kosambi?s mapping function) to the left of the markers positions. The vertical straight lines indicate markers placed at the same position ........................................................................... 41 Figure 2. An example linkage group with physical map contig identities integrated (LG19) via their BES markers. Square boxes of the same color indicate markers mapped from the same contig ....................................................................... 42 Supplemental Figure 1. A sex-averaged linkage map of channel catfish. Genetic map distance was given in centimorgans (Kosambi?s mapping function) to the left of the markers positions. The vertical straight lines indicated markers placed at the same positions.................................... 43 1 Introduction Channel catfish is the leading aquaculture species in the United States and an important research model for neurobiology, ecotoxicology, and immunology (Hansen et al. 2003; Booth & Bilodeau-Bourgeois 2009; Jemal et al. 2010). With the need to increase product quality and quantities, efforts over the last decade have focused on developing genomic resources for channel catfish for utilization in genetic improvement programs and functional studies (reviewed by Liu 2011), concurrent with the generation of microsatellite-based linkage maps for catfish (Waldbieser et al. 2001; Kucuktas et al. 2009) and catfish physical maps (Quiniou et al. 2007; Xu et al. 2007). However, these genomic resources have not been integrated yet because all genetic markers from previous studies coming from anonymous and gene-associated markers in which the location on the physical map is not known. Map integration aims to place a shared set of markers on both genetic linkage and physical maps such that one can relate genetic map positions and physical sequence. A high density of a genetic linkage map integrated with a physical map spanning across the whole genome provided a valuable resource for fine mapping of QTL and development of marker- assisted selection (MAS) programs, positional cloning of important genes, comparative genome analysis with similar species, and as a framework map for whole genome assembly (Palti et al. 2011; Palti et al. 2012). 2 Several methods have been used previously for the integration of the physical and genetic maps including: 1) in silico comparison of marker sequences to a whole genome sequence; 2) BAC pooling and PCR screening; 3) hybridization using overgo probes; and 4) mapping of molecular markers from BAC end sequences (Yuan et al. 2000; Coimbra et al. 2003; Romanov et al. 2003; Yim et al. 2007). In the last method, the most widely exploited markers have been microsatellites, which can be found in BES and used for genetic mapping to anchor BACs to linkage maps (C?rdoba et al. 2010). Integration of linkage maps with BAC-based physical maps has been carried out in some fish species including medaka (Oryzias latipes) (Matsuda et al. 2002), barramundi (Lates calcarifer) (Wang et al. 2008), Atlantic salmon (Salmo salar) (Lorenz et al. 2010), and rainbow trout (Onchorhynchus mykiss) (Palti et al. 2011; Palti et al. 2012), although the extent of integration varies among species. BAC end sequencing was carried out on the majority of the channel catfish physical map clones (Xu et al. 2006; Wang et al. 2007; Liu et al. 2009). The BES collection established a resource for mining polymorphic microsatellites which could serve as a connecting point between catfish genetic and physical maps. In catfish, 17.5% of BAC-end sequences (63,387) were found to contain microsatellites (Xu et al. 2006). Here, we have constructed the second generation of genetic linkage map using microsatellites and SNPs especially, utilized the BES resource for marker development and genotyping of BES microsatellites for the genetic linkage map construction and map integration. The integration of genetic linkage and physical maps of channel catfish using microsatellites derived from BAC-end sequencing will generate a strong foundation for comparative and functional genomics in catfish and a framework for forthcoming reference genome sequence assemblies. 3 Literature Review Fish and channel catfish Fish can be generally defined as craniates without cervical vertebrae. This characteristic is primary used for distinguish the different between fish and other vertebrates. Fish are extremely diverse when compared with other groups of vertebrates. There are approximately 32,000 species, and they are divided into 4 groups which are the hagfish (Myxini), the lampreys (Cephalaspidomorphi), cartilaginous fish (Chondrichthyes), and bony fish (Osteichthyes). The most common group is the bony fish constituting more than 95 percent of the world?s fish species (Schultz 2004; Eschmeyer & Fong 2012). The superclass Osteichthyes is divided into two classes, the ray-finned fish (Actinopterygii) and lobe-finned fish (Sarcopterygii). Actinopterygii is the dominant class of vertebrates, comprising nearly 96% of the 32,000 species of fish. They are ubiquitous throughout fresh water and marine environments from the deep sea to the highest mountain streams. Actinopterygii has three subclasses (Chondrostei, Neopterygii, and Cladistia). The subclass Neopterygii has two infraclasses: Holostei and Teleostei. Holostei are bony fish that show primitive characteristics. There are eight species divided among two orders, the Amiiformes represented by a single living species, the bowfin (Amia calva), and the Lepisosteiformes, the gars. There are more species to be found in the fossil record. Teleostei includes 20,000 extant species in about 40 orders; most living fishes are members of this group. 4 Channel catfish (Ictalurus punctatus) belongs to Order Siluriformes, or the catfishes. It is the leading aquaculture freshwater species in the United States. It belongs to the family Ictaluridae. The distribution of channel catfish was originally in the Gulf States and the Mississippi Valley north to the prairie provinces of Canada and Mexico, but they were not found in the Atlantic coastal plain or west of the Rocky Mountains. Currently, channel catfish have been found to be widely distributed throughout the United States and the world. One obvious physical characteristic of catfish is the presence of barbels around the mouth, four under the jaw and one on each tip of the upper jaw. The body is cylindrical in cross-section and no scales. Fin rays are soft except for the anterior dorsal and pectoral fin rays, which are sharp and hard spines. An adipose fin, lacking rays, is located on the back between dorsal and caudal fins. Irregular spots are on the sides of the body of young channel catfish. However, spots will disappear when the fish become adults. Channel catfish use their sense of taste to locate their food. Taste buds play an important role in finding food. They are usually found over the entire external surface, especially in barbels and gill arches, and inside the mouth. Olfactory organs, the organ of smell, also work along with taste buds for finding their food particularly in turbid water. Temperatures about 85? F (29.4? C) are the optimum for the best growth of channel catfish and the temperatures between 75?and 85? F (23? to 30? C) are suitable for spawning. Channel catfish usually become sexually mature at 3-4 years of age. However, some may spawn at the age of two years (Wellborn 1988). In 2009, catfish has been ranked at number six in most popular fish or seafood consumed in the United States. The average consumption of catfish in United States is 0.849 pounds per person (National Fisheries Institute, 2010). 5 Genetic variations Genetic variations are the variations of genome between individuals within a species or between groups of species. Two factors are involved in the uniqueness between individuals within species: one is deoxyribonucleic acid (DNA) and the other is sexual reproduction. These two factors are important to generate and control the genetic diversity within a species. In cases of DNA factor, genetic variation is the result of the DNA replication process. Although, proofreading mechanisms take place during DNA replication events, a few mistakes are unavoidable when such high numbers of bases are copied by adding the incorrect insertion of bases. These mistakes are called point mutations. Mutations can be induced in much higher rates with certain chemicals and radioactivity. With the nonexistence of such errors, no genetic variations would take place at the DNA level. These are affected to the adaptation of organisms if environment is changed. On the other hand, if these errors occur too much, organisms may not be viable and they can become extinct. In addition to variation at the DNA level, sexual reproduction can cause the genetic variation in organisms due to the shuffling of gene during meiosis in which recombination and independent assortment take place among chromosomes. These processes indicated that no gametes are identical to neither parent chromosomes nor one another. Besides, variations at the DNA level and the shuffling of genes during meiosis, genetic variations also occur at the chromosome level due to chromosome rearrangements. However, such variations are not very useful for developing genetic markers in aquaculture species, but they played a very important role in evolution (Beaumont et al. 2010). 6 Types of genetic markers The differences of genetic variation between individuals can be measured and quantified by several methods such as the directed sequencing of DNA fragments, measuring the differences between sizes of DNA fragments, identifying phenotypic differences (Beaumont et al. 2010). However, the most common methods to measure the genome variations were genetic markers. Some commonly used types of genetic markers are described as following (see Liu 2007) Allozymes Allozymes are the different forms of protein products of genes that are encoded at a single gene locus. Allozymes are considered to be Type I markers because they illustrate genes of known function (Liu & Cordes 2004). Allozymes polymorphisms are the result of point mutations, insertions, or deletions. Genetic variation of allozymes can be detected by electrophoresis. The applications of allozymes in fisheries and aquaculture are used for population analysis, stock identification, hybrid identification, parentage analysis, inbreeding analysis and limited genetic mapping (Liu & Cordes 2004). The advantages of allozymes are very cheap and technically easy. However, the major drawbacks of allozymes are that they require a large amount of fresh or frozen samples and in most cases the sampling for allozymes analysis is lethal. Additionally, the number of polymorphic loci is limited because of a very small portion of genetic variation, making the low polymorphic rate in allozymes. 7 Restriction Fragment Length Polymorphism (RFLP) RFLP is based on DNA fragment length differences after digesting genomic DNA with one or more restriction enzymes and separated on an agarose gel. Then, the DNA in the gel must be transferred to a solid support such as nitrocellulose or nylon membranes. The specific DNA locus of interest was hybridized with a specific probe, a radioactively labeled DNA or ribonucleic acid (RNA) molecule with sequence similarities to the locus of interest. After hybridization, the nonspecific probes must be wash away leaving only hybridized probe to the specific locus. The membrane is then exposed to the X-ray film for autoradiography to visualize the DNA bands. In addition to hybridization, another approach used for RFLP analysis is the use of polymerase chain reaction (PCR). This approach can replace the tedious Southern blot analysis because the RFLP region is directly amplified via PCR, and the PCR products can be directly digested with restriction enzymes. Then, the DNA bands can be visualized by simple staining with ethidium bromide. In cases where the size shift is small, polyacrylamide or sequencing gels should be considered instead of agarose gel. The molecular basis of RFLP polymorphism is based on insertions and deletions of large shifts in DNA fragment sizes and the gain or loss of the restriction sites. The major strength of RFLP is codominant markers. However, the weaknesses of RFLP are that it cannot detect the vast majority of point mutations and small sizes of insertions or deletions because of the low resolution of agarose gel electrophoresis. Thus, the polymorphic rates are very low in most loci. Moreover, RFLP detects genetic variation at one locus at a time as well as genetic sequence information is required. Therefore, it is difficult and time-consuming to develop markers in species lacking known genetic information. 8 Mitochondrial DNA (mtDNA) Mitochondrial DNA is double stranded circular DNA that is around 16 kb in length. In most cases, the offspring usually inherits mtDNA from its mother. However, some aquaculture species such as mussel, Mytilus spp., receive mtDNA from both parents. In animals, mtDNA has no introns, no junk DNA or repetitive sequences. Moreover, recombination is a rare event in mtDNA. Mitochondrial DNA was used as a genetic marker because the rate of mutation is higher than the normal nuclear DNA by about 5-10 times, and it represents significant variation among individuals. The rates of polymorphism are usually high in control region (D loop region), making this region highly useful for population genetic studies. Mitochondrial DNA can be analyzed using RFLP followed by gel electrophoresis. Mitochondrial DNA polymorphisms are caused by the loss or gain of restriction sites and also insertions or deletions. The major drawbacks of mtDNA are that it inherits in a non-Mendelian fashion. A total of genetic variation is from mtDNA alone. These features of mtDNA cause limitation for genome research studies. Randomly Amplified Polymorphic DNA (RAPD) RAPD is a PCR based multilocus DNA fingerprint technique. RAPD using the single short PCR primers (8-10 base pair [bp] in length to randomly amplify anonymous segments of nuclear DNA. Because the primers are short and low annealing temperatures are used. Thus, increasing in binding sites which distribute throughout the genome and generate a large numbers of amplification products in which each product represents different locus. Genetic variations are assessed by the presence or absence of each product. Base substitutions at the primer binding sites or the insertions or deletions within RAPD regions cause polymorphisms in RAPDs. The 9 polymorphism rates of RAPDs are relatively high because multiple sets of random primers can be used to scan the entire genome and generate multiple RAPD bands. However, polymorphic information content values (PIC) of RAPDs are lower than microsatellites and single nucleotide polymorphisms (SNP), and they are less informative than amplified fragment length polymorphisms (AFLP) because fewer loci are generated at the same time. Strengths of RAPDs are no need of prior knowledge of target DNA sequence and a large number of loci and individuals can be screened. Therefore, RAPDs are useful to differentiate various species, strains, lines, and populations. However, RAPDs are likely to have low levels of polymorphism among individuals of the same population and therefore they are not useful for parentage analysis. The major weaknesses of RAPDs are the low reproducibility due to the use of low annealing temperatures and the dominant nature of inheritance as well as the presences of paralogous PCR products from different DNA regions causing the bands appearing as a single locus. These limited the use of this marker system. Amplified Fragment Length Polymorphism (AFLP) AFLP allow rapid generation of large amounts of genetic information. Fingerprints can be used as a tool to identify a specific DNA sample or to assess the relatedness between samples. Conserved common bands refer to relatedness, whereas polymorphic bands refer to differentiation in phylogenetic and population genetic analyses. AFLP technology combines the advantages of RFLP and RAPD. It is based on the selective amplification of a subset of genomic restriction fragments using PCR. DNA is digested with restriction enzymes, and double-stranded DNA adaptors are ligated to the ends of the DNA fragments to generate primer-binding sites for PCR amplification. The sequence of the adaptors and the adjacent restriction site serve as primer 10 binding sites for subsequent amplification of the restriction fragments by PCR. Selective nucleotides extending into the restriction sites are added to 3'ends of the PCR primers in such a way that only a subset of the restriction fragments will be recognized. Only restriction fragments in which the nucleotides flanking the restriction site match the selective nucleotides will be amplified. Then, the subset of amplified fragments is analyzed by denaturing polyacrylamide gel electrophoresis to generate the fingerprints. The molecular basis of AFLP polymorphism is the same with RFLP which refer to insertions and deletions of large shift in DNA fragment sizes and the gain or loss of the restriction sites. The major strengths of AFLP are that it does not require prior genetic sequence information and it can generate a large number of polymorphism. Moreover, it provides robustness, reliability, reproducibility and efficiency. The weaknesses of AFLP markers are the dominant nature of inheritance, the difficulty of transferring the data among laboratories, and requiring a lot of technical demanding and special equipment such as automated DNA sequencers. Microsatellites Microsatellites comprise of multiple copies of tandem simple sequence repeats (SSR) ranging in size from 1-6 base pairs (bp) (Tautz 1989). Based on the number of repeats, microsatellites can be classified into mono, di, tri, tetra, penta, hexanucleotide microsatellites. When considered the types of repeats, microsatellites can be categorized into two types called simple microsatellites (AT)7 and composite microsatellite (CA)7(AG)9. Microsatellites are widely used as molecular markers because they are co-dominant markers (can tell homozygous or heterozygous and can tell allele frequencies), highly abundant, highly polymorphic, evenly distributed throughout the genome, and small locus size facilitating PCR genotyping. 11 Additionally, data can be used across the lab and sometime data can be used across species boarders. However, the disadvantages of microsatellites are the necessity to know sequence information, and the method is labor intensive to develop and has a high cost. Microsatellite polymorphisms are the results of the differences in number of repeat units between individuals. These variations produce the length variation of a given locus and variation can be detected by amplified locus using PCR followed by gel electrophoresis. Chistiakov et al. 2005 et al. (2005) suggested that two mechanisms might be involved in the change of the number of repeat units. The first mechanism is the interference during recombination events and the second mechanism is the replication slippage. Microsatellites with a larger number of repeats are likely to be more polymorphic. With as few as five repeats, polymorphism has been observed in microsatellites (Karsi et al. 2002). Because the large number of alleles per locus in microsatellites causes high heterozygosity and polymorphic information content (PIC) values, microsatellites became a widely used marker type in a wide variety of genetic research such as genome mapping, population genetics, parentage and kinship analysis, stock discriminations etc. than other markers. Since RFLPs, RAPDs, AFLPs, and SNPs are considered as biallelic markers, because of co-dominant their nature, microsatellites are considered as the most informative genetic markers. Microsatellites can be mined from bacteria artificial chromosomes (BAC) end sequences and they are usually type II markers because of more than 90% represented in non- coding regions. However, microsatellites can be developed as type I markers if they are developed from ESTs. Type I markers are very important because they are associated with genes of known functions. The most effective way to produce microsatellite type I markers is from cDNA libraries. However, some considerations should be given to mined microsatellites from 12 ESTs such as the presence of introns, the exon-intron boundaries, and 5' and 3' UTR regions. These make the flanking sequences are not suitable for PCR primer designs. Even through introns are not the problems for primer design of BAC end sequencing, the sequencing reactions often terminate after microsatellite repeats, also making the flanking sequences not suitable for PCR primer design. Single Nucleotide Polymorphism (SNP) SNPs are the most abundant form of polymorphisms caused by point mutation at a single nucleotide position in the genome. SNPs are inherited as co-dominant markers in a Mendelian fashion. SNPs can be categorized into 2 types: transitions and transversions. A transition is a change between two purines or between two pyrimidines. A transversion is a change between a purine and a pyrimidine. Transitions can be found more often than transversions. SNPs are biallelic markers, because in most cases only two alleles are observed for each locus, but, in theory, one SNP can have up to four alleles (Beaumont et al. 2010). In recent years, many researchers used SNPs as alternative markers despite the fact that they can be observed only as two possible alleles per locus (biallelic marker). This is because, compared to microsatellites, SNPs provide the cost savings, require short running time, has the advantage of high throughput screening, low genotyping error, ease in scoring, and speed in data collection as well as transferable data among laboratories. Moreover, SNPs present high abundant across the genome in both coding and non coding regions, and their mutation mechanisms are simpler than microsatellites. Most of SNPs are linked with coding regions that might be more useful in functional genetic studies than random genetic markers (Ryyn?nen et al. 2007). Since SNPs are biallelic markers representing the relatively low amount of information 13 per marker, a considerable amount of screening effort is required and a large amount of markers are needed to substantially develop and access large portions of genome. The drawbacks of biallelic markers of SNPs are likely to be the limitation in the non-model species because of the difficulty to obtain large scale species specific DNA sequences, therefore, much effort needs to be made through laboratories to discover and develop polymorphic markers instead of quickly scanning through polymorphic sites performed via computer (Morin et al. 2004). Another important issue when using SNPs to study population genetics in non-model organisms is the choice of a suitable platform that can be used for genotyping as many high throughput methods require expensive and specialized instruments not commonly found in non-model organism laboratories (Ryyn?nen et al. 2007). Thus, when considering SNP as a marker of choice for genetic research in non-model species, the investment in marker development, genotyping costs, and feasibility needs to be considered deliberately (Schl?tterer 2004). Furthermore, if SNPs are identified from small panels of a few individuals, rare SNPs will be under represented, resulting in some sort of bias (Nielsen 2000). However, SNPs also have some advantages over microsatellites as described above and with recent SNP genotyping technologies, allowing the detection of polymorphism from very small fragment sizes (Chen & Sullivan 2003). Genetic linkage map Genetic linkage maps are linear arrangements of genes or genetic markers on chromosomes, obtained by recombination (Liu 1998). They are valuable resource tools especially in detection of quantitative trait loci (QTL) for marker assisted selection (MAS). MAS can improve the production traits efficiently through the use of traits linked DNA markers and enhance accuracy in selection of desired traits. MAS was successfully implemented in many 14 economically important farmed animals, such as cattle (Maillard et al. 2003), swine (Rothschild 2003), poultry (Malek & Lamont 2003) and sheep (Notter & Cockett 2005). In aquatic species, MAS has not been implemented for the most part, although examples of successful marker- assisted selection have been reported (Fuji et al. 2007). In order to apply MAS as a tool for genetic improvement, a large number of polymorphic molecular markers are needed to construct genetic maps, and some of the markers should be linked to or are part of a gene associated with a desirable characteristic, such as growth rate or disease resistance. Genetic linkage maps have been constructed for a number of aquaculture species, such as tilapia (Oreochromis spp.) (Kocher et al. 1998; Agresti et al. 2000; McConnell et al. 2000; Lee et al. 2005), channel catfish (Ictalurus punctatus) (Waldbieser et al. 2001; Liu et al. 2003; Kucuktas et al. 2009), giant tiger prawn (Penaeus monodon) (Wilson et al. 2002), kuruma prawn (Penaeus japonicus) (Li et al. 2003), Japanese flounder (Paralichthys olivaceus) (Coimbra et al. 2003), rainbow trout (Oncorhynchus mykiss) (Young et al. 1998; Sakamoto et al. 2000; Nichols et al. 2003) and Atlantic salmon (Salmo salar) (Gilbey et al. 2004; Moen et al. 2004; Lorenz et al. 2010). However, the marker densities on these genetic maps are still too low to implement MAS. Due to low numbers of available molecular markers in many aquaculture species, MAS has not been extensively used in fish breeding schemes today (Sonesson et al. 2007). The only successful example is the MAS program in Japanese flounder for the selection of resistance against lymphocystis disease (Fuji et al. 2007). The major reason for the successful application of MAS in this species is because of the simple Mendelian inheritance of the resistance against the virus, while in most other situations, disease resistance is controlled by a large number of QTLs, and fully understanding of such complex traits requires a comprehensive genetic linkage map. 15 Although several types of molecular markers are currently available in genetic research, microsatellites are the most common markers that are used to construct genetic linkage maps and QTL maps in economically important species. Microsatellites are the most preferred markers because of their abundance in genomes, relatively even distribution, small locus size facilitating PCR-based genotyping, codominant nature of Mendelian inheritance, and high levels of polymorphism. In addition, microsatellites are sequence-tagged markers that can be used as probes for integration of different maps such as linkage and physical maps. Microsatellite markers can be easily transferred across the laboratories, and can be used across related species if flanking sequences are conserved (FitzSimmons et al. 1995; Rico et al. 1996; Cairney et al. 2000). In channel catfish, microsatellites represent 2.58% of the catfish genome (Xu et al. 2006). Microsatellite-based linkage maps have been constructed in some economically important aquaculture species such as Arctic charr (Salvelinus alpinus) (Woram et al. 2004), Atlantic salmon (Moen et al. 2004; Lorenz et al. 2010), channel catfish (Ictalurus punctatus) (Waldbieser et al. 2001; Liu et al. 2003; Kucuktas et al. 2009), Yellowtail (Seriola sp.) (Ohara et al. 2005), three-spined stickleback (Gasterosteus aculeatus) (Peichel et al. 2001), Nile tilapia (Oreochromis niloticus) (Kocher et al. 1998; Agresti et al. 2000), hybrid O. aureus?O. niloticus (Lee et al. 2005), rainbow trout (Sakamoto et al. 2000; Nichols et al. 2003), European sea bass (Dicentrachus labrax) (Chistiakov et al. 2005), zebrafish (Danio rerio) (Knapik et al. 1996; Knapik et al. 1998; Shimoda et al. 1999; Woods et al. 2000; Singer et al. 2002), Japanese flounder (Paralichthys olivaceus) (Coimbra et al. 2003), ayu (Plecoglossus altivelis) (Watanabe et al. 2004), Pacific oyster (Crassostrea gigas) (Hubert & Hedgecock 2004), Eastern oyster 16 (Crassostrea virginica) (Yu & Guo 2003) and common carp (Cyprinus carpio) (Sun & Liang 2004), cichlid fish (Astatotilapia burtoni) (Sanetra et al. 2009). In aquaculture species, genetic linkage maps have been used to undertake comparative genome analysis using conserved syntenic blocks corresponding to the model species thereby transferring important genome information (Kucuktas et al. 2009; Phillips et al. 2009). However, the major problem of map integration between species is colinearity because, during evolution, many of genes or makers can be rearranged and the evolutionary syntenic conservation, such as in catfish, is relatively low when compared with model fish species (Kucuktas et al. 2009). Physical map Physical maps are the linear order of genes or DNA fragments containing the order of overlapping cloned DNA fragments, usually obtained from restriction enzyme digestion (Liu et al. 1998). In order to understand the physical organization of the genome, most studies often study genomes through physical mapping using BAC libraries. BAC-end sequences provide the abundant information that can be used for physical mapping, comparative genome analysis and map integration (Xu et al. 2006) and also provide an unbiased survey of genomic sequences as well as relative abundance of polymorphic microsatellites in an organism. Currently, the physical maps of several important aquaculture species have been constructed. In channel catfish, a BAC contig-based physical map was generated using four-color fluorescence-based fingerprints. A total of 3,307 contigs, based on 30,582 BAC clones, were assembled by FingerPrinted Contigs software (FPC) using a cutoff value of 1?10?20. Each contig contains an 17 average of 9.25 clones with an average size of 292 kb. When combined contig sizes from all contigs, the genome size of the channel catfish was approximately 0.965 Gb, (Xu et al. 2007). Map integration Map integration is to place the same set of markers on both genetic linkage and physical maps such that one can relate the relative relationships of the linkage map and the physical map. Different methods have been used for the integration of the physical and genetic maps. They can be categorized as following: 1) in silico comparison of marker sequences to a whole genome sequence 2) BAC pooling and PCR screening 3) hybridization using overgo probes and 4) mapping of molecular markers from BAC-end sequences. In the last method, the most common and useful genetic markers have been microsatellites, which can be found in BAC-end sequences and used for genetic mapping to link BACs from a physical map (C?rdoba et al. 2010). In channel catfish, 17.5% of BAC-end sequences contain microsatellites (Xu et al. 2006) making it a valuable source of markers that can be used to construct genetic maps. BAC-anchored microsatellites are a valuable resource for genetic and physical map integration because they are identified through BAC-end sequencing and their location can be identified by BAC contig construction. After they are mapped onto the genetic linkage map, they allow integration of linkage map and the BAC-based physical maps. The map integration will be a possible framework for catfish whole genome sequence assembly, population and comparative genetic studies as well as catfish breeding programs. Integration of linkage maps with BAC cloned physical maps has been done in some fish species such as medaka (Oryzias latipes) (Matsuda et al. 2002), Barramundi (Lates calcarifer) (Wang et al. 2008), Atlantic 18 salmon (Salmo salar) (Lorenz et al. 2010), and rainbow trout (Onchorhynchus mykiss) (Palti et al. 2011). This study uses BAC anchored microsatellites as a majority of markers to construct genetic linkage maps because of their high level of polymorphism, high abundance, small locus size, highly distributed nature throughout the genome, co-dominance of inheritance, and sequence-tagged markers from BAC clones in physical map. Therefore, integration of the genetic map with the physical map is feasible. Additionally, microsatellites can be used for comparative genome analysis if they can be tagged to genome sequences, particularly within coding sequences as genes are well conserved through evolution. 19 Objectives The objectives of my dissertation are to construct the genetic linkage map using microsatellites and SNPs, especially BAC-anchored microsatellite markers to integrate the genetic and physical maps of channel catfish. The construction of the linkage maps with a high density of markers should enhance and increase the usefulness of the linkage map; integration of the physical map with genetic linkage maps should allow verification of the correctness of the physical map, clarification of the sequence assembly in the problematic regions, generation of the high quality genome sequences, comparative genomic studies with other teleosts and with model fish species, fine mapping of QTL, positional cloning, the development of a marker- assisted selection program and also increasing the potential as a research fish model species in teleosts. The inclusive integrated maps provide a minimal tiling path for genome sequencing and a whole genome sequence assembly in catfish. Therefore, the results from this project will be one step further to develop a marker-assisted selection and enhance genetic improvement program in catfish. 20 Materials and Methods Resource Family The resource family for BES markers used in this study was previously described (Liu et al. 2003). In brief, an F1 interspecific hybrid catfish was generated from the most informative mating of a channel catfish female and a blue catfish male. In 1997, backcross families were made using F1 fish mating with channel catfish (channel catfish backcross). A specific family, F1-2 x Channel catfish-6, was used for this project. Blood samples were collected from this specific family for genotyping. Heat brands were applied on each individual fish used for genotyping for future identification. Genomic DNA Isolation DNA was extracted from 64 samples plus their two parents from resource family F1-2 x Channel catfish-6. Blood samples (0.5 to 1 ml) were collected in a 1-ml syringe and immediately expelled into a 50-ml tube containing 20-ml of DNA extraction buffer (100 mM NaCl, 10 mM Tris, pH 8, 25 mM EDTA, 0.5% SDS, and freshly added proteinase K 0.1 mg/ml), and DNA was isolated by using the Puregene DNA Isolation Kit (Gentra Systems, Minneapolis, MN) following the manufacturer?s instructions. 21 Identification of Microsatellites, Primers and PCR Amplification Catfish BAC end sequences were generated by Xu et al. (2006). BAC end sequences (BES) stored in a local database at the Fish Molecular Genetics and Biotechnology Laboratory and available publicly in the NCBI GSS database and in the cBARBEL database (Lu et al. 2011) were used for microsatellite mining. The channel catfish BAC-based physical map, web FPC viewer version 2.1: AU 02-20 (http://titan.biotec.uiuc.edu/webAGCOL/AU02-20/webFPC) was used to obtain clones containing microsatellites which were previously identified by the Msatfinder program (Somridhivej et al. 2008). BES-containing microsatellites with at least 50 bp of flanking sequences on either side of microsatellites were used for primer design by Msatfinder. Primers were designed to amplify product sizes between 100-250 bp. However, in some instances, longer PCR products were accepted. A 19-bp tail sequence (GAGTTTTCCCAGTCACGAC) was added to the 5? end of the upper primer (Oetting et al. 1995). A primer whose sequence is complementary to the tail sequence is used as the label (labeled with infrared dye (IRD)-IRD700 or IRD800 from LI-COR Biosciences, Lincoln, NE). All primer pairs and the labeled primer were ordered from Invitrogen (Carlsbad, CA). PCR reactions were performed on a Mastercycler (Eppendorf NA, NY) or on a DNA Engine Thermocycler PTC 200 (Bio-Rad Hercules, CA) using the following amplification profiles: PCR was performed in a 5 ?l reaction mixture containing 1 ?l of 50 ng/?l genomic DNA (Gentra Puregene kit), 0.5 ?l of 10X PCR buffer, 0.2 ?l of 50mM MgCl2, 0.4 ?l of 2.5 mM dNTP?s, 0.2 ?l of 10 pmol/?l upper primer (with tailed primer 5'GAGTTTTCCCAGTCACGAC3' added at 5' end), 0.3 ?l of 10 pmol/?l lower primer, 0.1 ?l of 1 pmol/?l primer label IRD700 or IRD800 and 0.05 ?l of 5 U/?l of Platinum Taq polymerase. 22 PCR amplifications were conducted using 384 well plates to reduce the variation as much as possible. Two-step PCR profiles were used for amplification. An initial denaturation step at 94?C for 3.5 min was followed by a first denaturation at 94?C for 30 seconds, first annealing step at 57?C for 30 s, and a first extension at 72?C for 30 s. This first step was repeated for 20 cycles followed by 15 cycles of a second step with the following parameters: denaturation at 94?C for 30 s, annealing at 53?C for 30 s, and extension at 72?C for 30 s followed by a final extension step at 72?C for 15 min. The samples were held at 4?C for 15 min. In this two-step PCR profile, the annealing temperature window was accommodated to determine the best Tm for the primer pairs. PCR products were analyzed on a 7% polyacrylamide gel using a LICOR 4300 DNA Analyzer (LICOR Biosciences, Lincoln, NE). Genotyping After gel electrophoresis, the polymorphic microsatellite bands were scored and genotyped based on the allele segregation within the resource family. A Chi-square goodness- of-fit test was used to assess the Mendelian segregation patterns. Genotype configurations of markers can be categorized into three expected segregation patterns when null-allele segregation was allowed: 1:1:1:1-ratio type (? x ?: AB x CD or AB x AC), 1:1 ? type (AB x AA or CC), and 1:1 ? type (AA or CC x AB). All linkage analyses were made using JoinMap version 4.0 software (Van Ooijen 2006). 23 Microsatellite markers, type I microsatellites, microsatellites derived from next generation sequencing, and SNP markers Locus identifications and genotyping of anonymous microsatellites, type I EST and SNP markers were previously described in Waldbieser et al. 2001 and Kucuktas et al. 2009, respectively. Identification of microsatellites by next-generation sequencing (NGS) of the catfish genome was used for generating several NGS markers. Linkage Analysis Linkage between markers was examined by estimating LOD scores for recombination rate (?) and map distances were calculated using the Kosambi mapping function. Significance between linkage groups was determined using a LOD threshold of 14.0 and a threshold ? of 0.6 was set to detect suspect linkage possibly resulting from allele-coding errors. Markers were linearly aligned in each linkage group, converting recombination rates into Kosambi?s map distance (centimorgans). The position of markers was explored on the basis of the sequential buildup of the map (Stam 1993). First, the most informative pair of markers was selected, followed by sequential addition of other markers. The ripple, the action that prevented the adding locus to become trapped in the local optimum of goodness-of-fit, was performed each time after adding one marker. The best fitting position of an added marker was searched on the basis of the goodness-of-fit test (chi-square) for the resulting map. When a marker generated a negative map distance in the map or a large jump value in goodness-of-fit, which is the normalized difference in chi-square value before and after adding the marker, the marker was removed, and map calculation was continued to construct a first-round map. After the first- round marker ordering, the previously removed markers were added to the first-round map and 24 again subjected to the goodness-of-fit testing. In this manner, the marker ordering was continued up to the third round until an optimum order of markers was found. 25 Results Development of polymorphic microsatellite markers from physical map-associated BES markers We screened 30,582 BAC end sequences (BES) from BAC clones on the catfish physical map for microsatellites useful for map integration. The catfish physical map derived from fluorescent fingerprinting of the CHORI-212 BAC library contains 3,307 contigs (Xu et al. 2007). Ideally, at least one microsatellite from each of the 3,307 contigs, possibly more than one microsatellite for large contigs, is needed to fully anchor the physical map contigs to the linkage map. Our screening identified high quality microsatellites in BES associated with 2,128 of the physical contigs. The remaining physical map contigs could not be targeted by this approach, either due to lack of BES-associated microsatellites or microsatellites lacking sufficient flanking regions for primer design. A total of 3,846 microsatellites from the targeted physical map contigs were selected for PCR optimization and genotyping. Of these, 2,116 BES microsatellites from 1,534 unique physical map contigs amplified a single product of expected size and could be resolved and unambiguously scored (Table 1). These potential BES microsatellite markers were carried forward for use in construction of the genetic linkage map. 26 Linkage Mapping To construct a high-density genetic linkage map incorporating the BES markers, we combined the 2,116 BES microsatellites derived from the physical map contigs with 77 BES markers from BAC singletons as well as 100 SNP markers, 283 EST markers, 147 markers from a previous linkage map (Waldbieser et al. 2001), and 17 microsatellites derived from next- generation sequencing for mapping on our interspecific hybrid resource family, F1-2 x Channel- 6, using the Joinmap 4.0 program. The majority of the EST and SNP markers were utilized in an earlier linkage map genotyped on the same resource family (Waldbieser et al. 2001; Kucuktas et al. 2009). To start the linkage analysis of the sex averaged map, the LOD score was set at 14.0 in order to generate the linkage groups based on the segregation data. Among 2,740 polymorphic markers, 2,557 markers, including 2,099 BES-based microsatellites, 235 EST- derived microsatellites, 127 anonymous microsatellites, 17 next generation sequencing-based microsatellites and 79 SNPs, were mapped and assigned to 29 linkage groups. A total of 93.3% of genotyped markers could be successfully mapped (Table 2 and Supplemental Figure 1). The remaining 183 markers were not mapped because of significant segregation distortion, unlinked markers or other mapping abnormalities. Marker Distribution and Map Characteristics In the resulting genetic linkage map, markers were distributed across all 29 linkage groups with a minimum of 46 markers/LG (LG29; see Figure 1 for a representative LG). Similarly, the linked physical map contigs were distributed across linkage groups, ranging from 33 to 77 contigs/LG (Table 3). However, distribution of markers was uneven, likely reflecting differences in recombination frequency along the length of the catfish chromosomes 27 (Supplemental Figure 1). Clustered marker regions were observed in every linkage group of the sex-averaged linkage map, especially in positions close to the centromeres and, more infrequently, at the telomeres. In order to characterize marker clustering, we tabulated the number of markers sharing an identical genetic map position with other markers as well as the number of map positions where markers were stacked or redundant (Table 3). Linkage group 17 had the highest percentage (72%) of markers placed at the same location, whereas linkage group 19 had only 14.5% of its markers at stacked map positions (Table 3, Figure 1). In order to obtain a more accurate evaluation of marker distribution and map density, we calculated the number of unique markers per linkage group by subtracting the markers at stacked positions (identical cM location) and adding back the number of stacked marker positions (to account for one unique marker per stacked position). A total of 1,836 unique marker positions remained. We then utilized unique marker counts for each linkage group along with estimated linkage group sizes (with corrections for telomere ends) to calculate an average LG density (cM/marker). Densities ranged from 0.5 cM/marker in LG17 to 2.6 cM/marker in LG29, with an average of 1.4 cM/marker. Of note, LG17 represented the skewed corrected map size at only 16.8 cM, likely reflecting the severe shortage of recombination events observed in this linkage group. In contrast, the telomere- corrected average size of the 29 catfish linkage groups was 87.8 cM (Table 4). The sum of intervals from all loci of the sex-averaged map after excluding stacked markers was 2,467.9 cM (Kosambi). To correct the chromosome ends (telomeric regions), the estimated total map size was calculated by adding 2x, where x is the average space between adjacent markers, to all map sizes of each linkage group (Fishman et al. 2001). Therefore, the estimated corrected map size of the sex-averaged map was 2,546.2 cM. The average number of 28 mapped markers per LG was 63 markers, and ranged from 31-87 markers. Inter-marker distance varied from 0 to 43.7 cM (Table 4). Evaluation of Map Integration Of the 1,534 physical map contigs with at least one successfully genotyped BES marker (Table 1), 1,481 physical map contigs, approximately 69.6% of physical map contigs with microsatellites, were successfully integrated with the linkage map (Table 5). Due to multiple BES markers being successfully mapped from some physical map contigs, a total of 2,030 contig-based BES were placed in the linkage map. Detailed information regarding mapped contig identities, estimated physical contig sizes, corresponding linkage groups, and genetic map positions is listed in Supplemental Table 1. In terms of the physical coverage of the integrated genetic map, the integrated physical map contigs include a total of 17,972 individual BAC clones. Fifty-four physical map contigs, each with at least two BES markers, were mapped to multiple linkage groups based on conflicting assignments of individual BES markers, suggesting that these physical map contigs may be erroneously constructed or that they fall within duplicated regions of the genome (Table 5). An example of a linkage group with physical map contig information integrated based on marker position is illustrated in Figure 2. Given an estimated haploid genome size of channel catfish of 1X109 bp (Tiersch et al. 1990; Tiersch & Goudie 1993), and a total corrected map size of 2,546.2 cM, we calculated the relationship between physical and genetic map distances to be approximately 393 kb/cM. Additionally, the combined estimated physical length of the 1,481 anchored physical map contigs was equal to 510,074 Kb (Table 5), the integrated map covered ~ 45% of the physical 29 map contigs and ~51% of the estimated catfish genome. Anchored contigs varied in size from 88 Kb to 2,206 Kb with an average size of 344 Kb (Supplemental Table 1). A total of 322 contigs were mapped with multiple markers and observed recombination between at least one pair of markers, allowing contig-based estimations of ratios of physical to genetic distance (Supplemental Table 2). Physical distances were estimated based on position of the BES markers within each contig. Multiple marker contigs were distributed across all 29 LGs. Ratios ranged from 4 kb/cM to 7,704 kb/cM with an average of 485 kb/cM. 30 Discussion The aim of this study was to integrate a BAC-based physical map of channel catfish with genetic linkage maps by genetic mapping of large numbers of BES-associated microsatellites. In this process, we also sought to increase the marker density and utility of the catfish genetic linkage map for genomic studies. Here we mapped 2,030 BES markers from 1,481 physical map contigs to integrate a substantial portion of the physical map and to boost average marker density to 1.4 cM/marker. Map integration is a critical component of efforts aimed at marker-assisted selection in aquaculture species. Fine-mapping of QTL is often thwarted by an inability to identify genomic regions containing causative genes and/or additional markers in linkage disequilibrium with the locus of interest. With an integrated map, markers of interest can be related to BAC contigs which can then be quickly sequenced using next-generation sequencing approaches to obtain the genic content and structural organization. Additionally, whole genome assembly can be significantly enhanced using an integrated map to orient and order sequence scaffolds along the length of linkage groups based on their matches with BES. Significant gaps revealed during this process can be filled by targeting BAC clones within the gaps for further sequencing. Furthermore, prior to whole genome assembly, information captured through ordering of physical map BES can be used for comparative genomics studies (Yu et al. 2009; Palti et al. 2012). Assembly of catfish genome sequence scaffolds is currently underway and will utilize the map integration data presented here. 31 The utility of genetic and integrated maps is directly correlated with the even distribution of markers across linkage groups. Clustered markers in areas of minimal recombination, while allowing general linkage group assignment, often cannot be used for definitive fine mapping and positional cloning. Physical map contigs placed within these regions similarly cannot be ordered and oriented in relation to other nearby contigs. In fish, clustering of DNA markers on genetic linkage maps has been observed in medaka (Naruse et al. 2000), rainbow trout (Robison et al. 2001), tilapia (Kocher et al. 1998), channel catfish (Liu et al. 2003), and Atlantic salmon (Lorenz et al. 2010). While, potential explanations for high levels of marker clustering are not completely understood (Watanabe et al. 2005), most are based on dead zones of recombination. Numerous studies have reported large disparities between genetic map and physical map distances (e.g. Young & Tanksley 1989; Gustafson et al. 1990; Gustafson & Dille 1992; Espeso et al. 2005). This phenomenon is often attributed to differences in genic content across chromosomes, with recombination decreasing in chromosomal regions containing heterochromatin, large areas of repetitive DNA, and in regions around centromeres and/or telomeres. The catfish genome is known to have long repetitive regions including Xba elements and abundant transposable elements that likely interfere with recombination (Liu et al. 1998; Xu et al. 2006; Nandi et al. 2007; Jiang et al. 2011). We observed significant clusters of markers on the majority of the catfish linkage groups. However, some linkage groups were clearly more impacted by this phenomenon than others. For example, linkage group 17 had 67 markers (out of 93 in total) in 5 stacked marker positions, while linkage group 19 had only 8 markers (of 55 in total) in 5 stacked positions. It will be of interest to characterize the repetitive content levels in linkage groups with differing rates of recombination as genome assemblies become available. Of note, a previous EST-based linkage map genotyped on the same resource family had far fewer 32 stacked marker positions (Kucuktas et al. 2009). While marker number in the previous map was lower, selection of markers from coding sequences likely helped to avoid highly repetitive genome regions. Several other potential reasons for low recombination in these regions deserve exploration. The number of genotyped progeny may impact rates of observed recombination between genetic markers. We examined whether the use of 64 fish for genotyping provided insufficient power to detect rare recombinants. We genotyped 128 fish (64 original plus 64 additional family members) for 93 BES markers found within highly clustered map regions. Recombination rates rarely increased substantially from those seen using 64 fish (data not shown), indicating that progeny number was not limiting in these cases. It has also been long noted that recombination is reduced in hybrids due to decreases in homology and reduced pairing of chromosomes during meiosis (Young & Tanksley 1989; Kreike & Stiekema 1997). This leads in turn to linkage drag where large, non-recombining fragments are retained in backcrosses, an issue long confronted by plant breeders (Rhyne 1962). The use of a hybrid backcross family in the present study, therefore, may have increased the extent and size of cold zones of recombination. In the future, genotyping of clustered markers on an intra-species mapping panel may increase resolution within at least some of the low recombination regions. The clustered BES markers, while of minimal utility in fine ordering of physical map contigs for QTL analysis within these regions or assisting in whole genome assembly, do provide gross assignment of physical map contigs to linkage group regions. The ratio of physical to genetic linkage distances varied substantially across the 29 linkage groups and between markers within the same physical map contig, again illustrating differing rates of recombination across the catfish genome, as seen in other vertebrate genomes 33 (Nachman 2002; Nievergelt et al. 2004; Quiniou et al. 2007; Groenen et al. 2009). Ratios calculated on a linkage group level are impacted by the presence of non-BES markers in the analysis. These markers (ESTs, SNPs, and others) contribute to the genetic map distance, but are not part of the physical distance estimation based on contig lengths and thereby skew the calculated ratios. To avoid this issue, we focused on physical to genetic distance ratios based on multiple markers mapped from the same contigs (Supplemental Table 2). Ratios ranged from 4 kb/cM, potentially indicating hyper-recombination, to 7,704 kb/cM, indicating suppressed recombination. Several contigs showed no recombination between markers. A combination of approaches will be utilized for integration of the remaining 1,826 contigs not integrated in the present study because of lack of sufficient microsatellite sequences or insufficient polymorphism in existing microsatellites. For some contigs, we are in the process of identifying SNPs within BES regions (Lorenz et al. 2010) for mapping. Other contigs will be integrated through connection of BES sequences with SNP-containing genomic regions via initial catfish genome assemblies (Liu et al. 2011). These SNPs in turn will be genotyped on large reference families utilizing a high-density Affymetrix SNP chip. As the catfish genome assemblies improve, additional contigs will be integrated through the physical sequence connection of genotyped markers and contig BES. Recent rapid improvement in both sequencing and SNP genotyping technologies presents many new options for achieving full integration of the catfish genome resources (Baird et al. 2008; Davey et al. 2011; Van Oeveren et al. 2011; Scaglione et al. 2012). Although variation in recombination frequencies complicates the relationship between genetic and physical distance, integrated maps are valuable for checking for errors in individual map assemblies. The majority of contigs with multiple markers were placed into a single linkage 34 group. However, 54 anchored contigs were mapped to multiple linkage groups. This could indicate FPC assembly error during physical map construction resulting in mis-assembly of genomic regions. Increasing stringency of assembly of these regions may resolve the issue. However, in some cases, these contigs may be the result of assembly of duplicated regions of the catfish genome which share high levels of sequence similarity (Xu et al. 2007). The breaking of these contigs during linkage mapping is of great utility in highlighting these potentially duplicated regions. We examined whether increasing stringency for FPC construction of the physical map could resolve the assembly in problematic contigs. We have increased the cutoff values for the contig assembly to 1x10-25 and 1x10-30, respectively. The majority of problematic contigs (37 out of 54) were split from the original contigs into different contigs or singletons when increasing the threshold, while 17 of 54 contigs remained in the same physical map contigs. Whether a BAC contig is to be split into different contigs when assembly stringency is increased depend on the level of overlapping segments generated from restriction fingerprinting. Apparently, the 17 contigs that remain in the same contigs with increased assembly stringency indicated that long stretches of duplicated genome segments may have been involved in these contigs. Such information will be important for the assembly of whole genome sequences. Additionally, in a few instances, contigs whose BES markers span an unexpectedly large genetic map distance (>10 cM), may also indicate mis-assembly, or intra-chromosomal duplications. Such large discrepancy between linkage map and physical map distances could also come from genome variations as the resource family used for genetic linkage map is not related with the fish used for physical mapping. Given the large variation in recombination ratios across the catfish linkage groups, however, these determinations will be more difficult to make at the present time with the available genome resources. 35 Conclusions The catfish integrated map presented here substantially increases linkage marker density available for QTL studies as well as connecting the majority of the catfish genetic markers to physical sequence BAC contigs through the use of BES microsatellites. The integrated map will allow trait-based studies (e.g. Ninwichian et al. 2012) to expand beyond linkage analysis to fine mapping and selection of important candidate genes for further research. The integrated map will also be a valuable asset in improving and validating the catfish whole genome assembly currently underway. 36 Table 1. Development of physical map contig-specific microsatellites from BAC end sequences. Number of contigs 3,307 Number of contigs with microsatellites 2,128 Number of contigs with polymorphic microsatellites 1,534 Number of polymorphic microsatellites in the 1,534 contigs 2,116 Number of contigs with two or more polymorphic microsatellites 385 37 Table 2. A summary of markers used for construction of genetic linkage map. Marker type Attempted Polymorphic Mapped Microsatellites from BES 3,846 2,193 2,099 SNPs 384 100 79 Microsatellites from EST 1,025 283 235 Anonymous (IpCG) 268 147 127 Microsatellites from NGS 20 17 17 Total 5,543 2,740 2,557 38 Table 3. Characteristics of marker distribution among 29 linkage groups. Linkage Group Mapped markers Contigs Stacked markers Stacked marker positions Unique marker positions 1 113 77 56 15 72 2 84 49 19 7 72 3 107 74 41 14 80 4 104 70 48 11 67 5 98 59 33 10 75 6 126 74 89 13 50 7 89 47 44 12 57 8 119 75 55 16 80 9 91 50 43 11 59 10 68 36 11 5 62 11 89 60 36 9 62 12 101 58 29 8 80 13 100 57 32 10 78 14 81 43 18 7 70 15 101 52 33 12 80 16 98 55 32 10 76 17 93 52 67 5 31 18 71 45 25 6 52 19 55 41 8 4 51 20 121 73 44 10 87 21 74 43 15 6 65 22 80 50 39 8 49 23 78 52 32 9 55 24 60 38 22 5 43 25 87 57 31 8 64 26 76 42 27 10 59 27 65 35 21 8 52 28 82 47 21 10 71 29 46 33 14 5 37 Total 2,557 985 264 1,836 39 Table 4. Characteristics of the catfish genetic map with a total of 2,557 markers at 1,836 unique map positions among the 29 linkage groups. Linkage group Unique markers Map size (cM) Total map size (cM) Average marker density (cM) Range of intermarker spacing (cM) 1 72 76.6 79.3 1.1 0-12.9 2 72 79.9 82.6 1.1 0-6.6 3 80 99.9 102.6 1.2 0-14.9 4 67 88.3 91.0 1.3 0-17.1 5 75 70.0 72.7 0.9 0-6.6 6 50 71.6 74.3 1.4 0-17.6 7 57 78.3 81.0 1.4 0-15.9 8 80 127.8 130.5 1.6 0-43.7 9 59 70.3 73.0 1.2 0-13.0 10 62 98.0 100.7 1.6 0-13.2 11 62 83.9 86.6 1.4 0-22.6 12 80 94.5 97.2 1.2 0-10.0 13 78 114.2 116.9 1.5 0-11.7 14 70 87.0 89.7 1.2 0-13.0 15 80 93.7 96.4 1.2 0-12.6 16 76 111.6 114.3 1.5 0-11.4 17 31 14.1 16.8 0.5 0-2.0 18 52 73.6 76.3 1.4 0-21.8 19 51 90.9 93.6 1.8 0-10.9 20 87 91.0 93.7 1.0 0-13.5 21 65 73.2 75.9 1.1 0-8.9 22 49 89.2 91.9 1.8 0-15.1 23 55 87.7 90.4 1.6 0-12.6 24 43 69.2 71.9 1.6 0-15.7 25 64 84.5 87.2 1.3 0-7.4 26 59 57.5 60.2 1.0 0-5.2 27 52 100.8 103.5 1.9 0-6.0 28 71 93.4 96.1 1.3 0-8.8 29 37 97.2 99.9 2.6 0-18.3 Total 1,836 2,467.9 2,546.2 1.4 - 40 Table 5. Assessment of integration of the catfish physical and linkage maps. Number of contigs with polymorphic microsatellites 1,534 Contigs mapped to linkage map 1,481 Number of BES microsatellite markers mapped from the 1,481 contigs 2,030 Contigs mapped with only one microsatellite marker 1,096 Contigs with two or more microsatellites mapped 385 Contigs with two or more microsatellites mapped at the same position 191 Contigs with two or more microsatellites mapped at adjacent map positions 140 Contigs with two or more microsatellites mapped in different linkage groups 54 Average length of integrated physical map contigs (kb) 344 Number of BAC clones contained in the 1,481 contigs 17,972 Total number of BAC clones on the catfish physical map of 3,307 contigs 30,582 % of BAC clones on the physical map integrated with linkage map 58.8% Total length of integrated physical map contigs (kb) 510,074 41 Figure 1. An example linkage group (LG19) from the genetic linkage map of channel catfish. Genetic map distance is given in centimorgans (Kosambi?s mapping function) to the left of the markers positions. The vertical straight lines indicate markers placed at the same position. 42 Figure 2. An example linkage group with physical map contig identities integrated (LG19) via their BES markers. Square boxes of the same color indicate markers mapped from the same contig. 43 1 Supplemental Figure 1. A sex-averaged linkage map of channel catfish. Genetic map distance was given in centimorgans (Kosambi?s mapping function) to the left of the markers positions. The vertical straight lines indicated markers placed at the same positions. 44 2 Supplemental Figure 1. Continued. 45 3 Supplemental Figure 1. Continued. 46 4 Supplemental Figure 1. Continued. 47 5 Supplemental Figure 1. Continued. 48 6 Supplemental Figure 1. Continued. 49 7 Supplemental Figure 1. Continued. 50 8 Supplemental Figure 1. Continued. 51 9 Supplemental Figure 1. Continued. 52 10 Supplemental Figure 1. Continued. 53 11 Supplemental Figure 1. Continued. 54 12 Supplemental Figure 1. Continued. 55 13 Supplemental Figure 1. Continued. 56 14 Supplemental Figure 1. Continued. 57 15 Supplemental Figure 1. Continued. 58 16 Supplemental Figure 1. Continued. 59 17 Supplemental Figure 1. Continued. 60 18 Supplemental Figure 1. Continued. 61 19 Supplemental Figure 1. Continued. 62 20 Supplemental Figure 1. Continued. 63 21 Supplemental Figure 1. Continued. 64 22 Supplemental Figure 1. Continued. 65 23 Supplemental Figure 1. Continued. 66 24 Supplemental Figure 1. Continued. 67 25 Supplemental Figure 1. Continued. 68 26 Supplemental Figure 1. Continued. 69 27 Supplemental Figure 1. Continued. 70 28 Supplemental Figure 1. Continued. 71 29 Supplemental Figure 1. Continued. 72 Supplemental Table 1. Detailed information regarding contig identities, estimated physical contig sizes, corresponding linkage groups, and genetic map positions. Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2582 34 490 1 0.0 GCATGGGAGGCATATCTCAG CTGACCTTCCTGGCTGTTCT 067B2C11 AUBES1157 536 430 1 3.0 ACACACACTCAGCCTGTTGG TGGAGCCACTGTACTCGTGC 008A1F09 AUBES2148 1702 183 1 3.0 AAACATTGCGTGAGAGACCA ACGGATGTGGTGAGCTTCTC 022B2A04 AUBES4076 716 418 1 4.7 CACGCTTACTTCGGACAGTG AAATCTCTCCTCGCCTCCAC 060B1H12 AUBES2400 1702 183 1 5.8 TGCAGTAATCAGTTGTGGGAAT GCCAAGACTTTTGCACCCTA 045A1G01 AUBES1995 1970 225 1 8.8 ACCCATACCTTGTGCTGACA ACCCGGACAACATGACAGAT 027A1G08 AUBES5192 2099 352 1 9.9 AAAGGTTTGCTCTCCCATTG TGCACAAAGACAGACTGACACA 110A2A11 AUBES4012 1191 267 1 14.7 TGTAAGCTCCATCCCCAGAG TCAGATGAATGGACGAGTGC 042A1C05 AUBES4851 1749 348 1 16.8 AATCATTGAGCTGGCAAAGC TGTCTGCACTGTTTTTCATGC 084B2H08 AUBES3298 478 289 1 21.8 TCAAAAGCTGGGAGGTGAAC TCTTGCAATTACGCTTGCAC 054A1A03 AUBES1924 42 1038 1 22.6 GGAAACGAATGGAAAGCAAA GTGAATTCGCCTCTCTCCAA 036A2D04 AUBES4943 636 377 1 22.8 GATGAAGCAATGCTGCTTGT GTCCTGGCCTGGTACAACAT 049B1B01 AUBES3946 150 362 1 23.0 CCAAAAGGCGTTCTAAGCTG CCCTCCAAAAACATTGGAAC 014A1G10 AUBES4697 3006 230 1 23.2 CATGGCCGAAATTTGTATTTG CACCGTGGCATTCATTACAG 003A1H09 AUBES3566 767 254 1 23.5 CCGAAAGTGGACCTTTCAAC CAAATATGGAAAGGAGGCTGT 044A1G01 AUBES3598 1289 683 1 23.8 AGCACAAAACTGTGCAGGTG TCGGATTCTCCAGTAACTCCA 029B1G06 AUBES2756 249 365 1 24.3 CCAATCTTCCATGCAAGTCA GAACAGGGATGTGAGGGAGA 019B2A08 AUBES2772 597 654 1 24.5 GGAAGGCAGCGTCTAGAGAA CCTTCTCTGAACCTCTGAACC 020A2F03 AUBES5202 2155 249 1 24.7 AGTTTGGAGAGACGGAGCTG TTCCGATTTTGAGCACACAG 074A1C08 AUBES3178 316 775 1 24.7 GTGACAGGAAATGCAGCAGA CACTACTGTTTGTCGCCAGAAT 053B1E04 AUBES4022 1283 359 1 24.8 GAGCCTATCCCAGGGAACTG TTACGGGGTTTACCTTGTGC 091B1F06 AUBES3350 149 714 1 25.0 GTACCGATGCCACCAAAAGT GTGTGCGTTTAGGAATGCAG 034A1H08 AUBES2927 1258 359 1 25.1 TCCACAAAGGCTCTAAGCAC CTGAAGCCCTGAACCAAAGT 032B2B08 AUBES2764 428 516 1 25.1 TGGGACAAGCAGATTGTGTG GGATAACCGCATGAATGAGC 048B2F11 AUBES4720 3271 166 1 25.2 ACACATGGGGACATTTGGTT TTCCTCCTTTGCGCAATAAC 018B2H06 73 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3568 772 311 1 25.4 ACTTCAGCCATTGAGGAGGA CCATCTCACATGTTGCTTCC 003A2C01 AUBES3160 370 891 1 25.4 TCATGTCATGGAGGGTTTTT CTCCACTCAGACATGGGACA 060A2E06 AUBES4540 2276 235 1 25.4 GTGGCTTTACCTCTGGGACA GAGCTCATTCAACCGACTTG 078B2H04 AUBES2634 42 1038 1 25.5 TCCTGAGCTGCTGTGAGTTG TGGTGTCCAGGAAGTGTTCA 064B2B12 AUBES4992 1011 345 1 25.5 GGCAACAATGTTTGATTTGC CCAGGCAAGACTGGAGTAGC 050A1E07 AUBES5191 2097 194 1 25.5 ATCGGTGGTAGTTTCCAAGC GTGCCAGTTTGTTGCGTACA 008B2A11 AUBES4729 2071 216 1 25.5 TTTTTCCACCAATATGACATGA TAAGTGGGTGGGAAATGACA 033A2G08 AUBES4437 1272 216 1 25.6 GCACAATAATGTGGTGGGAAA CGCTTTCTCTTTGTCCTGCT 087B2H07 AUBES4099 1037 227 1 25.6 GCACTGAGGTTTCAGTGAGC TCTCTGCCTCAGACCGACTT 011A2A05 AUBES3451 949 384 1 25.6 GCATAAAGGAACAGAAAATTACCC TCTGTTGAATACAGCACCAGAA 066A2H10 AUBES4436 1272 216 1 25.6 GACAGCAGAGGTGGCCAATA AACAGCACTCTTGCTTGTGC 023A1C09 AUBES4263 1331 355 1 25.6 TGATGGACACTCCTCATGAAC TGCATGTCTGTCAGAGCAAGTAT 013B2H05 AUBES2839 167 313 1 25.7 TTCTGACCCTGAGCCATTCT GGTGCACTCATCTCTGTTGC 031A2G09 AUBES3475 251 257 1 25.7 AGTCTCCAGTGCCAGTGCTT CCTTCACCAGCCTTTCTTGT 062B2G06 AUBES4139 1122 186 1 25.7 CCCCACTTCCAGACTATTCCT ACCATGATGAAGCGCTAATG 075A2E08 AUBES4860 1940 230 1 25.7 CTTGTTGTGAGTGGGTGTGC TGCATTACCCTGTGTTTATGTT 030A1A10 AUBES4764 2719 262 1 25.7 GAAGGAGTCTCCGGTGTCAG CGGTCATTCTTTCACCAACC 041B2F02 AUBES4800 3020 183 1 25.7 AAGGAGTCTTGAGTGTCAGTGC TGATAGGGCCTGATGGAAAG 001B2G04 AUBES5165 1839 181 1 25.7 CCTACAATTGTTGATGTGGTGAA GTCTCTACCCACCCAATCCA 026A1C04 AUBES4861 1940 230 1 25.7 GGGACTCCCATCAGAAACAA TGCCTGTTGTTCAATCTCAA 087A1D01 AUBES1691 350 318 1 25.7 TGTCATGTACAAGAGCAACCTC CACTCCTCCCTCTGCAACTC 034B2C08 AUBES2627 370 891 1 25.7 CCATACCCAGATGTCTGCAA ATTGGCCCTGGTTATGAATG 035A2A01 AUBES3565 767 254 1 25.7 CCCATGATTGGCTAATGTCTCT CGGGACTCCATGAGCACTA 014A2B10 AUBES5120 1561 266 1 25.7 GCAGGATGAGGCTCAGTGTT GACATTGTGGGTGTACTTATATACCTT 036B1B08 AUBES4650 718 306 1 25.7 GCCTGTCTGTTGGTCTTGGT AGACAGGCAGAAAACATACAAGAA 029A1A02 AUBES3923 1163 365 1 25.7 TGTCAGCAAAGCCAGATGTC TATGGGGTGGGGTGTAGAGA 075A2E03 AUBES3452 949 384 1 25.7 TGGTAAGCCTCTGTTGAGTGA GGTGTAGGCAGTTCCAGCTT 087B2E05 AUBES2049 2717 149 1 25.7 AGGATCACCCAGCTGTCCTA TGCATCCTACACACGCTCTC 039A1F06 AUBES2954 633 311 1 25.8 CACACAGCGTTAAGCACACA TGTGCAGAAAACTGTGCTGTC 024A1H01 74 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2358 597 654 1 25.8 AAGGCTGTGTCACCATCACA GCATGAGCGACATGAACTTG 047A2H03 AUBES3567 772 311 1 26.0 ACTTCAGCCATTGAGGAGGA CCATCTCACATGTTGCTTCC 007A2E09 AUBES4624 2169 215 1 26.0 CCCGTTTTCTTTTTATGCTTGT ACACTGATCCCCAAGTTGCT 107A1F01 AUBES3209 316 775 1 26.0 CATGAATGAGAGGGCATCAG GATGCCAATCAAAGCAGGAT 032B2G10 AUBES2446 2106 184 1 26.2 AAATTCGGCCAATGCTACAG CTTCCAGAACACAGCCACAA 040B1A04 AUBES4641 448 245 1 26.2 CCTCCCATAGCGTGGATCT CTTGGGAAGCTGCATCAAAT 004A1H02 AUBES2953 568 514 1 26.3 TGTGCCAACAGACCTCACTC CTGGGCTCTTGGATTTGACT 051B1G11 AUBES5057 1352 240 1 26.5 CTCCAAATGCACATTGCTGA CAGTTATTAATTAGTTTGGCGTGTG 014A1F03 AUBES1267 1612 227 1 26.7 TGCCAACATAAAGAGAAGCTGG CTCCGCCGTTCAGTGCGAGG 001B2H05 AUBES1803 597 654 1 26.8 AAGGCTGTGTCACCATCACA GCAGTACGACATCCACCTGA 034B1F10 AUBES4541 2276 235 1 27.1 CTCCAACCTCATCAGGCATT TGCAAACACAAAACAGGTGTG 088A1C07 AUBES5061 1368 335 1 27.2 TTCAGGGCACAGCTTAATTG CAACCCATGGATATGCTCCT 108A1H04 AUBES5198 2135 266 1 27.5 CAGGATGAATATGGCGAGAG CACCTGTACTCACCTGCCATT 076A1E10 AUBES4468 1153 181 1 28.1 CATTAGGCCTAGGGGATGGT CGTGTCCAAAGGGTCCATAG 106B2C06 AUBES3108 42 1038 1 28.6 GCATGGGCTGCGTAGTTTA ACCCGTGTTTTCCGATACAG 029B1H10 AUBES5183 2000 255 1 29.2 CCCATAACGACAACGTGAAA GCGACAGTCAAAGTCTCCAG 107A2C09 AUBES2110 singleton 1 30.1 TTCAATTCCATCCGATGTTG CAGCTCTTCAGTCTGCTCCA 041B2B05 AUBES5119 1560 249 1 33.6 GCTGTTAACGTTAGAATTTGATTTT TCATCTTCGTGTGCTGCTTC 005B1A12 AUBES3993 2142 352 1 37.8 CCAATCAGGACATCCACCAT GACGCCCGCAAATCATATAC 087B2A02 AUBES5317 2745 200 1 38.8 TGCTTTCTGTACCGGTTTCAC GTATGAGTTTCGGGCTTGCT 038A1H12 AUBES3421 468 348 1 39.3 TTCTTCATGCCTTGCTCTAAG GGGCATGCCTTGGTTATATG 077B1H08 AUBES3420 468 348 1 39.4 GCGCTTAAAAGCAAATAGATTG GGGCATGCCTTGGTTATATG 007B1H07 AUBES3992 2142 352 1 39.8 CCAGCCATAATCACTGTGGA AGGGAACACCATGATCAGTACC 008B1G02 AUBES3745 2239 617 1 40.0 ACACCCCTGCTCTGTCACAT TGAAATTCCACAATCCCACA 092B1A12 AUBES3752 2239 617 1 40.1 ACACCCCTGCTCTGTCACAT TGAAATTCCACAATCCCACA 108B1E11 AUBES4483 1542 171 1 40.1 TGTAGACTGAGCTTTGTGGTTCA CATTGCTGTAGGGCTCTTTACAT 055B2D03 AUBES3688 504 311 1 41.9 CCCACCATTACAACACAGCA ATCTGGCTCCCTCCTACCTC 048B1B04 AUBES2695 690 524 1 42.0 ATGGAGCGCTCATCCAGA TCGGTGGGTCTGTTTGTATTC 013B1C09 AUBES1792 2734 259 1 42.1 TATGCACACCCAAGGTGGTA CACCGGGTTTTCTGTCTCAT 033B2A01 75 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4321 2019 238 1 44.5 GGAGTGTCCAGTGTCAGCAG GTGGAACTGATTGTTGGAAGC 106B2A07 AUBES4724 3364 166 1 44.8 GCATTAAGCTTTCAGCTGTGG TTCACGCAAACATACATTCG 074B1C06 AUBES2082 1924 198 1 47.8 AGCTGGGGGAAAAGAAGAAG TATTCCCTCGGACACAAAGC 041A1A08 AUBES3403 351 299 1 48.3 GCCGTTGGAGTCTTTGACAT CCCTTTGCTTTCTCTCTCCA 007B1F09 AUBES3404 351 299 1 48.3 AACGCTGCAATGTGGATTTT CGGTGTTCTTTATGTCCACCA 039A1A04 AUBES5344 3228 271 1 48.3 GCTCCAGCAAGAGACTGTCC GCTCAGGTCGCTCATACGAT 019A2B12 AUBES1378 2666 205 1 48.4 CCAGTGGACAAAGCCTGC CAGTCCTGACTTGCCAGG 034A1G06 AUBES1597 2328 455 1 49.5 TGGAGCAAACAACGTTTGAG AAAGCGATTCCCCATCATC 023A1A10 AUBES5327 2928 208 1 50.2 CGACTTTCGGCCAATAAAAC CAGGTAAGGCCCAGTGAGTT 014A2D09 AUBES4310 1934 337 1 51.0 CGTTAAGGCTGCTCCAGATT AGGGAAAGCACACGACAGTT 104B1G04 AUBES4758 2670 154 1 51.9 TGTTTGATGGACGTTGATGG CACATGGGTATGCCAATCAC 060B2D12 AUBES4414 2439 381 1 68.2 CAGGACCTTCTGCTCACCAT GCACTCGTCTTTCAGCCTCT 102B1B04 AUBES1075 2439 381 1 69.1 AAGCAAGCCTGGACCATGAC GTGCTCTGTTAGCTGGAGTG 010A2A02 AUBES1263 singleton 2 0.0 AGACGTATGGGCGAGATCAG CTGACCTGAGCACTGGTGTG 033A2D02 AUBES1868 singleton 2 0.5 GTTCTCCAGGCCCAGTCAT TCCTCTCCTGAGATCCTTAACA 024B1A09 AUBES4268 1378 418 2 1.9 GTGCACTATCTGCCAGCATC CGTCACTCGTCACACTTAGC 001B1F02 AUBES4170 1693 496 2 3.8 AAATCGGTCGGTTCACTGAG GGGCGAAAGACGTAAAGGAG 110A1D08 AUBES1947 1693 496 2 4.7 CAGATCCTGATCCCTGATGG TGGACTCTGCCTTTTGATCC 019A2D06 AUBES3080 236 795 2 6.0 TCCTCTGGAAGGCTCTCAAC TTCTGCTTGCACCAAATCAG 092B1H10 AUBES5080 1448 289 2 6.7 CAATAATGCGATGGATGGAT CGCAGACGTTCTGTGACATT 012B1G04 AUBES4698 3007 178 2 11.0 ACCACTGGGTGCTTTCTGTC TCCAGAAGCGTGTCACTGTC 072A2G03 AUBES2884 343 661 2 13.2 TCACTTTCCTATGGCCTTGTG ATCCACTCTGTGGTCGGTTC 028A1A11 AUBES2885 371 409 2 13.6 GCTGTTAAACCATTGCCTGA ACCCTGCCTGCTTAGTGAAC 074A2G06 AUBES4165 1514 445 2 16.7 GCTGCTGCCAGTTTATGGAT CCTTCATGCCTCATCACGTA 109A2F02 AUBES1881 3007 178 2 19.4 TGTCACTTAAATCACGGAGGTT ATCGGGCCTTGAGGTAGATT 027B2B03 AUBES3952 343 661 2 20.6 AGCGGATGGAAAGCTCTAGG GGTCCGATAAATAGCCAACG 096A2E11 AUBES3395 145 352 2 26.4 GCCAAAATGTCTTTGGAGGA CTACGGTGACATTTGGGTCA 042A2B09 AUBES2258 145 352 2 26.5 TGGAGCAAAACATCCAACAA GTGACGGGAGAGGATCTCAA 042B2F02 AUBES3626 337 250 2 33.1 ATGCATGCAATTTACCCACA CCCTAAGGCTCCAATAAGTGC 055B1C09 76 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3394 145 352 2 34.3 GTGTTGACGCTGTAACACACA AGCGAATTTATCTGGGGCTA 063A1F02 AUBES1586 1524 296 2 35.4 TCCTCTCATCATCACCACAGT CAAAACGCCAATGACATGAT 019B1G08 AUBES5410 3534 166 2 35.6 TGAGCTTCTACTCAGCTGTTGG ATCACGCAGCTGCCTAAGTT 096A1A11 AUBES3625 337 250 2 37.0 CAGAGCTACGAGCAGAGCAT CCGTACTGGAGCGGAGTAAG 073A1C09 AUBES4162 1513 450 2 39.0 GTGCCCTTTGGAAAACTGG CACATGTTGGAAGCAGACCA 045A2F12 AUBES4789 2915 242 2 39.6 CGAACACACTTGATCGGCTA TCCCTTTGGTGAACAGGAGT 062B2A07 AUBES4487 1572 205 2 39.7 TGGCAACTGGAACAGTGGTA CGGGTGATGGATAGATGGAT 075A2G11 AUBES5146 1690 188 2 40.1 GCACGCTTTTAGTCTGCAAAT TGAACTCGCACCACACATCT 030B1D11 AUBES1592 2904 125 2 40.4 CCCTTTAAAGAGGGCTTTTCC GGTGCGTTCAAGAGAAAGGA 029B1A08 AUBES3627 407 294 2 40.6 AGTCTGGTGAACGGGTTTGA CAACACCTACCATTAGCCACCT 049A1A10 AUBES5044 1296 194 2 40.9 TGGCACGAGTGTGGTTTCTA TGCCAGAATGAACACTTCAACT 053A2F10 AUBES5300 2659 232 2 41.3 CCGGAAGTATTTGGTTGCAG TGAAACCGTGTGTCTGTGCT 061A1C12 AUBES3633 544 293 2 41.8 GTCGGGTTCCTCTCAAGGTT TTGCAAACACAACACAGTGC 006A2E07 AUBES3628 407 294 2 41.9 AGCCAATGTTTCTGGACTCG CTGGAAACAGACTCCACACTGA 020B2G10 AUBES4163 1513 450 2 41.9 TACATGTGGCTGGAATGCAC CCTGTGTCCAGGTTCTCCAG 046A2D02 AUBES4434 998 200 2 41.9 GTTCGAATTCGCCTGCTATC GCACAAGGAGTTGTGGGAAA 063B1G01 AUBES2279 2365 266 2 41.9 TCTGCAGAGGTCAGGTAAGGA CTGCAACGTTCTAGGGAGGT 043B2A03 AUBES4435 998 200 2 42.0 CGAATTCGCCTGCTATCTGT GCACAAGGAGTTGTGGGAAA 090B1A08 AUBES5387 3406 198 2 42.0 AGTCAGTGCAGCTGGTTCAA GGCCATTTGCCTTAATGTCT 038A2H03 AUBES3758 373 266 2 42.2 CCGAATCATTCTCGGATCAC TTCGACTCTTTGAGCCGATT 032B2A11 AUBES3082 68 668 2 42.3 TACACACAGCCTTCCCATCA TGAACATCCAGCCCAGTTATC 003B2A10 AUBES3858 71 468 2 42.4 AACAAAGCTGGCTGGGTATC TGATGAGTGGGCCACATAAA 084B2D07 AUBES3036 456 445 2 42.4 GGATAAGAGGGAAGAGATGAGC TACGCAGCACAATGGACAAT 058B1H03 AUBES3238 14 404 2 42.5 CGTTGATTCCTTTCATTTGG TGGCTGCCCTATGAAGTTAAA 081B1C05 AUBES5001 1082 337 2 42.7 TCCTCACGCTTGCCTATTTT CCAGTTTTCAAACAGTCAGTCC 072A2D11 AUBES1701 1745 167 2 42.9 CAGCAAGAGAGGGCACTACC GCGCCTGTACATAACTGCAA 034A1C10 AUBES2676 14 404 2 43.3 GCTGGAACGTTTCTGGCTAA ATCGCTCGCTCTCTGTCTGT 003A1F03 AUBES3988 1998 284 2 45.1 GGCTAGGCCCAAATAAAAGG TCAGCAGGGGATCAAATACT 052B1H07 AUBES3978 1777 323 2 45.1 AGAACAACGACCAGGGACTC TGTGGACTTGTCTTGCGAAT 001A1G07 77 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4111 1386 318 2 45.4 TTTGCGTTACAGCAACATGA TAATGCAGCCCAAATGTGAT 065A2D06 AUBES1842 singleton 2 46.2 CCACCTTCCACTCAGGAGAC GAACATATCTGGGCCTTCCA 036B1H10 AUBES2085 570 533 2 46.5 TGGAGACGTGCTCTAAAACTCA CAGACTGAGCCACAGGACAG 041A1C09 AUBES1853 singleton 2 46.5 CTGGAGAAAGAGAATGGAGCA CTCCCATTCCCCACACAG 036B2A12 AUBES1615 570 533 2 46.7 TGATGCCTGGCTGAGAGATA TCCCTTAAAGCCCTCAATCA 024B1H04 AUBES5241 2393 188 2 46.9 CAAACCGGAAGTTCTGGAAA GCACTTCCAGGCAAGACACT 080B2E08 AUBES5412 3537 164 2 47.5 GGAGCTGCAATAGGGCTTT TTGAAGTCTGAATTTGCTGCAC 085B1C11 AUBES4269 1414 423 2 47.6 AGTGACGTCATGCTCCGAGT ATGCCCCCATTACTGTGAAC 051A2A09 AUBES4457 1706 233 2 51.3 TTTCACTGCAGAACCTGCATA TGTAACTTGTCGATGGCGAAT 078A1F03 AUBES2036 803 259 2 52.5 AACGCCGGATAGTGTGAAAG CGAGTGCGTCTTTGTAATGG 038B1G11 AUBES4456 1706 233 2 53.1 CAGCAAGCTAACGGTGATGA GCCCAGGAACAAAAACTGAA 072A1E03 AUBES3183 55 614 2 55.4 GAAGCATTGTGGTGCATTGT CCAGTGTTTTCCAGGGTCAT 107A1H11 AUBES3184 121 956 2 55.8 TGGGAGTTAACCAGGGAAAG GAGGTTTTTCTCCCCAGTTG 058B1C03 AUBES4633 2173 352 2 56.4 CACGTTTCGAAAATAATATCAGGA GTTTTGCTCCTCCTTCCAAA 035A1F05 AUBES3215 121 956 2 59.0 TGTGTAGCCAGAAATACAGATGC GACAGCCAGGCACAGAGATA 014A1E01 AUBES5046 1311 353 2 63.9 CAGAACTCAACAGCAGTGTGG GATCGTCCAATTCAGCATCA 086B1D08 AUBES3995 2151 269 2 64.9 TCTTTTCGTAATAGTTGCGTCCT AAAGAATGAATTGGGGTCCA 053A2F06 AUBES4858 1930 430 2 70.2 CTGTCGATGATGTCCTCGTG GCTGCAGTCCCTCACTACATC 108A2C12 AUBES5408 3523 173 2 70.2 AACTTCCAAGCAGTGCGGTA CGAGAGCACTTAAACCCTGAA 063A2A08 AUBES4859 1930 430 2 71.1 CAGCACGCAGAATCCTGTTA GTGCGATCAGTCGAGGATTAC 084A2A07 AUBES2551 singleton 2 71.9 GGTCCTAATGAACACGACGAA CTTTGCCGCTAAGTCATGGT 049B2H01 AUBES5332 3096 198 2 72.5 GCGTCCTTGAATGACCAGTT GTCACGTACGAGGAACGATG 061A2D09 AUBES2152 737 731 2 79.9 CGGCTGACTGAGGATTTGA AATCGCAAGCTGGAGAATCA 023A2A10 AUBES4824 3154 129 3 4.6 TTTCTCATTGCAGTGGATGC CATTTTCAATGTGGTCTCAGACAT 062B1B04 AUBES1073 2216 107 3 6.5 ACCAAAATGTGAAGGGTGTTCTC TGCTACTCTGTTGGTGCCAG 010A1A06 AUBES5124 1601 282 3 9.0 GTTTGTGGTGCCATGTCAGA TGAGGATGACTTTGAGCCTTT 080A2D03 AUBES5154 1746 218 3 9.0 ACTAAACACTGGGCCCCTTT TGAATGCAGGAGCACAATTT 039A2C05 AUBES4476 1408 274 3 30.2 ACCTGTACGGTCGTCACTCC TTAAGCTATTGCTCCGTCCA 090B1F08 AUBES4740 2401 211 3 30.9 TAGGGGTTCAAGCAAAATGG TTCTTCCTCGAATCCCTGTTT 104B1C05 78 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4718 3240 276 3 32.0 CAGAGTCTGTGAGCGGTCTG CCAAAAAGTTTGAACTGAATCG 090B2G03 AUBES4690 2892 266 3 33.4 AAATCACGCTGGAGGGTTC ACCAGCCACAACTCAGATCC 070A2B03 AUBES5331 3076 213 3 37.1 TCCATGTCATATCGTCCACCT TCAAGCTCACTCCAACATGC 038A2D11 AUBES3895 1604 223 3 38.6 TAAATGCAGGTGTGGACTCG AAGCGACAAACTAGCAAGTGAA 038B2A08 AUBES4395 1869 237 3 42.2 CTCAGGCATTACAGCAGCAG AGCCCTGTGACCTAGTGAGG 109A1C12 AUBES5169 1882 174 3 42.8 TGGACGAGTCATGAATTAGGC CAGACAGTGTCCTTCTGCTTG 012A1C09 AUBES1811 3484 238 3 48.3 CCTGAACTCACACTGGCAAA TTCCGTATTCTGGGGTGTGT 033B1A08 AUBES5048 1326 272 3 48.5 ATCACCACCGTCTGACCAAT CCTGTTGAGGAGAGCCAATG 087A1A04 AUBES5168 1867 301 3 49.4 GGGTTGCCAGATCAGAACAG TGTGTTGGTCCATGATCGTT 012A2C03 AUBES3536 227 475 3 51.8 CATTCCCTTGCTCCTTCATC TTGAAATGGATTTCCCATAGC 052B1H01 AUBES1186 2527 129 3 54.0 GATTAGGGCACTGAAGTCACG AGACCCCAGCCAAGCTGTCC 011B2C12 AUBES3065 227 475 3 54.1 TGTTGAGGTGTCTAGGATGCTG AAAAGGGCCTGGCTAATTGT 058A2C07 AUBES1590 684 387 3 57.7 TGTCAACTTGGACCTAATGTGC CGCTTTCATTCGAAGCAACT 023B2G12 AUBES3544 377 340 3 58.4 GGGAAATATCACAGCCAGGA AACGTTCATCCCATGTCTCC 011A1C06 AUBES4343 97 599 3 58.6 GGTGGGTTGCTCACAAGTTT CAAATGCCGGTATGTGGATT 053B2A05 AUBES3771 919 276 3 59.4 TAGCATTCAAACGCCCTTGT TTTGGGCTCAAACTATCTCCA 079B1B12 AUBES3074 97 599 3 61.0 GGAAGTTACCTGCAAAACACC TGCTTTCAACAGTGTTTCCAAC 033B1A12 AUBES2347 335 744 3 61.7 AGTCAAGCAAAGACGTGTGC GAGGCCGAAGTGTCTCTGTC 047A1F03 AUBES1846 477 330 3 62.1 GTCACGCTGATGACTCTCCA CACTCATTCAGTGACAGGAACTT 036A1C10 AUBES2748 128 416 3 62.4 ACAGTTTTCCGCCATGGTAA ACCACTGTTGGGCAGCTAAC 001B2A09 AUBES1742 486 440 3 62.9 GCCCAGGGGTCCAATTAC TGCGTAGGTCAGATCCCTCT 020A1A02 AUBES1781 62 318 3 63.0 CGAGGCGAGCTGGTTTAATA TTTACCTCTTTTCCCGCTCA 033A1A07 AUBES4784 2884 167 3 63.6 TCATGAAAAGGCAGGTGTTG GAGGTAGCATTCATGCACAA 112A2H10 AUBES4254 1186 286 3 63.6 CTTATGTTGCTGAGGAGTCTGG TTGGTTAGGGGAGGTTAGCA 031A1G04 AUBES4350 377 340 3 63.7 TGCCCTAAAAAGCTTTCCCTA CATTATGGGTGCTGTTCACCT 063A1B05 AUBES2595 335 744 3 63.7 CCGGGTCTCTCAGTCTCTCA GTGCAGACATCTCCAACACC 009B2C08 AUBES5148 1705 457 3 64.8 TCCTAGTGCCAGGCTCCTAA GTGGTCAATCACCCACCTG 088B1D05 AUBES4950 683 200 3 64.8 TGACATTTATTCCCCCACTGA TTCCAAACAACCAAGAGGAAA 076B1D08 AUBES2555 480 709 3 64.8 ACTGTTGTTGCTGTTGTTGTTG GCACAAACTGACAAACAGATTCA 022A2G04 79 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4702 3060 198 3 64.8 TGTGATTGAACCCTTCAGACC CTCAGTGGCTTGCTCAGTTG 064B2E11 AUBES4769 2793 123 3 65.0 TGTGGATTATGGGATGGATG CCCATTCAAACAGACTTAGGTG 009A1D07 AUBES2052 819 409 3 65.2 GAAAAGCCTCCCTCATACCC CAGCCCAACTACAATGAGCA 039A1H03 AUBES2815 307 320 3 65.4 GCACGACTCAAAAAGCCACT GCATCAGCCAAATGAACAAA 027A1E08 AUBES3646 789 279 3 65.5 TGTAACGGCAACGGTTTGTA GCTATGGTTAACGAGCTGGA 086B2A10 AUBES5229 2353 281 3 65.5 GGATTTCATCCATTCCAGCA CTGGGAAAGTTCAAGGGTCA 109B2F09 AUBES3879 919 276 3 65.5 GCGACTGAGGGGAATAATGA ACACACACACACATGCTCGT 045B1E12 AUBES4462 888 201 3 65.6 ACATGTTTGCAATGCCACTC TGGCAGCTCTGCTACCACT 034A2F08 AUBES3327 480 709 3 65.6 GCCCGTAAAGGATTTGTTCA ACGATCCCATCCACACTGAG 070A1D09 AUBES4271 1438 365 3 65.6 GGTGAAGGCATGGAGGTTAC GACCTCTGCACCTTCCTCCT 014B1D11 AUBES1697 1459 335 3 65.6 CAGCACACGAAATTGTCCAG TGGAGAACACAGAAAATCTTGC 033A2C01 AUBES4044 1504 331 3 65.6 TGTGTCATGGCTCACACTATG GTTTCTTTGAGGAGCGCTTG 086B1H07 AUBES3139 335 744 3 66.1 TCATGATCAGGGTTCATCACA GTGTCCGCTTAGTGGGTTTC 107A1C03 AUBES3880 919 276 3 66.2 CAGCACTCTGCTCTCGTGAA GCCCCCTCACCTCAATAAA 096A1A03 AUBES3647 789 279 3 66.2 TCTTGAGCATTTCCCAGGAT CGACACACATGGCATACACA 100B2D04 AUBES3491 477 330 3 66.5 CACCTGAAGGATGAGCGAAC TGTCACAGTAAGCCTCCGGTA 021A1E05 AUBES1626 singleton 3 67.5 AGTGATTGCACATTCACAAGG TGGTACACGATCATCTTCCTGA 027A2E03 AUBES2984 305 470 3 68.0 CAATCTCTGCACAGCCACAT GCTTGTTGGTTAGCTCATTCA 106B1A08 AUBES2097 1192 216 3 68.8 GCATCCTTGAGTTCTGGAAAA AGTCTGCTGTGGGGAACACT 041A2F01 AUBES5176 1937 186 3 71.2 GTCATGCAGCTGAAGAGGAA AACCTTCAGGCCATTTCTGA 109A1E06 AUBES2550 988 382 3 72.3 CACCTCCTTAAGGTGCATTGA TGTAGACTTGGCCAGAAAGGA 049B2G08 AUBES4830 3185 223 3 72.8 AGATTTTCTCATGGGGAGCA CATTCCTGCAATCTGCCAGT 013B1G04 AUBES2926 1205 340 3 73.2 ATGGTGATGTGGTGGATACC TTGGCCCAAGGTACAGACTC 048A2H02 AUBES2086 571 589 3 73.3 CCACCCCCACCTCTATCTCT CTCTTTCCCCTGATATTCAAGC 041A1C12 AUBES5336 3198 413 3 73.9 TCTGGGAAATTGCACAGGAT AATAGCTGCAGGGGAAAATG 090A2B05 AUBES2423 136 514 3 74.3 TTATCCCTATACACGAGCACATAA TCCTTGGCATCCATATTTCC 045B2G01 AUBES4578 1893 189 3 74.3 TGGAAGCAGGAAGAGTGACC ACTGGGTCAGCTGGAGTACC 085A2G03 AUBES4593 1893 189 3 74.4 TGGAAGCAGGAAGAGTGACC ACTGGGTCAGCTGGAGTACC 085A2G03 AUBES4228 679 266 3 74.5 GAGCTGTCTGCACCTAAAGGA TGGTGAGACAATTCGCAAAG 108A2D09 80 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5232 2363 191 3 74.5 GCTTCGGTACAAATGCATGA GCACAGTAACTGGACAATTGGA 045A1H04 AUBES5259 2489 140 3 74.5 AATACGATGGTGATATTCAAACG CAGAACAGCCTTTCACATCG 010A1G02 AUBES4876 2932 328 3 74.6 TCTGGCCCTTTGACATCTTC TACCCGCATTCTCATGTCTG 051B2G06 AUBES5297 2650 210 3 74.6 GCAACCCTGTTATGCAATGA CAGCAATTTAAGCACTGGGAAT 096B1E07 AUBES4970 859 326 3 74.9 GCATCTGACCGAACAACAAC TATAGGAATGCCCACCTGGA 080A1H02 AUBES4875 2932 328 3 75.2 CGTAAGATAACCAGTGAATGTGATG AGCTCCTTCCTTGTGTGTCC 001B2A11 AUBES3913 1070 255 3 75.4 CAGGAGTGCTGGGTATGAGG TCTCCCCGGGTTTTCTCTAT 062B1F09 AUBES4502 2532 274 3 75.9 TTAACTCTGCCTCGCCCTTA CATAGCCACCCTAGCCAGAT 100B1B09 AUBES2113 2292 147 3 76.2 TTGGCCTATCAAAGCACAAA GCTTCATGCAAGGTTTCCTC 041B2C10 AUBES2091 1211 259 3 76.9 GCTGTGTGTGCCCTCATCTA ATGACCACACCCTTCTGACC 041A1H09 AUBES2095 1776 573 3 77.0 CTCTGCCCTGTGGAAGTTGT CAGCAGCAGGTGATTTTGAG 041A2D04 AUBES1963 412 272 3 77.6 TCCTGCTCTTGCTGTCTTGA GAATACTTTCCGGATGCACTG 026B1C03 AUBES4974 882 316 3 79.8 AACGGAGTTGCATTATGCTG GATGTGCACCCTTATCGTCA 072A1A04 AUBES2411 1128 516 3 79.8 ACCCTGACACAAGCTCAAAT GAGTGACATCTGCTGGTGGA 045A2F07 AUBES5067 1393 288 3 79.8 CGCAGAATAAAATGCGCTCT ACTTCTAGGAACGCGCACAC 035A2D12 AUBES1381 476 460 3 79.9 AGTTCAGTTCCGCAAGCTG ACCTGAATTAGGAGGCTACCTG 023B1F03 AUBES4976 889 475 3 80.8 GTCGTTCGTGCACTTGTCTG TCAGTCGCAGTCTGTCATCC 101A1H05 AUBES2958 827 587 3 81.4 GTGTGAGGCGAACAACTAAGC CGTCAAATCTGGGTACTGTCG 013A2B07 AUBES3443 702 379 3 81.6 CAACCTGCAGCACAAACATT AACAGGCAGCTGCATTCAT 065B1H10 AUBES2837 127 359 3 81.7 ACGGCATGTAACGAAGGTTT CATTTGTATTTAAAGCAGCACATCA 020A1B08 AUBES3444 702 379 3 82.0 TGGAAGCGGTAACGAAACTT CGTGAACTGAGGAGTGGCTA 110B1F11 AUBES2421 702 379 3 82.0 ATAAGCGGCAGAACAACAGC AGGAGATCCGTATGGGTTTT 045B2F09 AUBES4704 3085 142 3 83.1 TGCCGGTAGGTGCCTAAAAT CGGTTAAGGCACATTGGACT 102B2G01 AUBES2915 852 411 3 85.0 CTGACAAAATGCACACCATTC CTTCAGCGAAAGCTCGTCTT 007A2E03 AUBES4620 2153 269 3 99.9 CCTGTCAAATCGGAGACGTT CGACAGGGGCTTTGAATATCT 079B2B08 AUBES1740 3 397 4 0.0 AACCCTGAACTTAAAACCCTGA CAGTGTCAGCATCCAAGAGG 032B2G01 AUBES5273 2543 222 4 17.1 CGTCGAACATGAGGACAGC CCTTGATTTCGCTGCTGATT 083A1F07 AUBES2353 singleton 4 18.1 TTCCTTTAGTTCCTGGAAACG GCGTTCTTATCAGCGGTCTT 047A2B08 AUBES1795 670 228 4 23.2 GCCGGATCACCATCAAATA CCACACACTCAGTTGGCAGT 033B1C07 81 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4570 1803 222 4 26.4 TTCTTCCCTTCCTTCCTTCC ATGCAGGGAATTGGTGAGAG 072A1E08 AUBES4936 578 347 4 28.6 CAGACGTTTGGACCCCACTA CGCGCTACATCACCAAGATT 111B1C01 AUBES1327 1302 254 4 31.5 AGGGTCCTCCAACAGGCTCG CTCGCATCAGCACCATCTCG 031A1C08 AUBES5203 2162 213 4 35.4 CAGGGTTTATTGGAGCTCGT CAGCCAATCAGAAATTCAGAAG 028B2F04 AUBES5266 2521 196 4 35.5 CTCGGCAAGTGACATGGAT TGTCAAGCTTTACTGAGCATCTG 006A1D06 AUBES5234 2374 122 4 37.1 CCCCCATCCTCATACTGATT CACACTGCTACCATTCATCTCA 051A2G06 AUBES4904 234 301 4 37.7 CTCATCCGCGCAGAAATTA GCACACATATTCAAAGCGTGA 060B1C02 AUBES2399 singleton 4 45.3 TGAAAGAATGATGCTTGTGTGA GCTTGTGGCGTTAAAAGAGG 045A1E11 AUBES5177 1950 352 4 52.8 AATTGGGCAAACAGGACAAA TGGTGTCCTTGCTTTAGGTG 064B1D06 AUBES4460 792 169 4 54.1 AGCTCAGCCAGTCACTCCTT CAGCAGTCACCATTTACACTCA 104B1A09 AUBES1153 1323 342 4 54.5 GTTTATGGGTAACCCTGTCAAGG AAGGAGCTGTCCTGTTCACC 007B2A11 AUBES4496 2356 220 4 55.5 TTGCTTTAGTGTTTCGTGAATTG GCAGATCCCACCCTAACTTG 030B1H04 AUBES4257 1223 413 4 55.7 CCACTCACCTGTGCCTGAAT CTCCACGCACACAGAATATGA 030A1C12 AUBES4565 1756 222 4 56.0 TCAGTTGACTTTGGGGGAAA TTGTGCTATTGTGACCTGCTG 111A1F11 AUBES3780 1491 325 4 56.4 GCCTGCATAATGGATGGAAG AACCCAGCTCATAAGCCAAG 027A1C04 AUBES4354 544 293 4 56.8 GTTAGCTGCAAGGGAATCAA TCCATGAAATGTCCGCATAA 046B2C04 AUBES3638 676 387 4 56.8 CAGCTTGTTGCTTTCCCTTC GCGGCTGTATCATTTCAGAG 086A2D04 AUBES4743 2501 250 4 57.0 ATGACCCTGACCAGGAAATG TCATAGCCCTAGCACAAGCA 053A1C04 AUBES1413 2046 299 4 57.0 CCCACGACCCTGTAGGATAA CGTGGATACTGCTCTGCGTA 026A1C02 AUBES2792 703 250 4 57.5 ATACGATTGGGCTGCTTTTG CCCTGTAGCCATCAACTTGC 050B2C01 AUBES5397 3463 206 4 57.5 GGATCCTGCACTCAGTCGTT CAAGAGCATCCACTCACCAA 045B2D11 AUBES3639 676 387 4 57.5 CAGCTCAAGGGTTGTCATCA ACGGTGACAGGAAAGAGGTG 044B2B04 AUBES4036 1415 291 4 57.6 CCTCTTTCTCAAGTTGGTTGG CGAAGTCAGGGACCAGAAAC 045B2A08 AUBES2427 94 462 4 57.8 TCCCCTCTGTTTCCTGTCTG TGACAGACCCATGCATTTAATC 040A1D04 AUBES1841 911 509 4 57.9 TCAAGGACTTTCCGTTCCTG TTTGGTGAGAACCCCTGAAG 036B1C01 AUBES1810 1597 553 4 57.9 TGTGCAAAACCAGCACTTTC TTGCATTTGGCCATGAATAA 033B1A06 AUBES4084 787 171 4 57.9 GACAAGACGATTTAACGCAGTG CCTCACACTCACCACTCAGC 061A1D12 AUBES1230 1721 284 4 58.0 TGTGCTCCCGTGGACCTCAG CCAACAGTCATCTGTCTGAGC 022B2E01 AUBES4083 787 171 4 58.0 GACAAGACGATTTAACGCAGTG CCTCACACTCACCACTCAGC 036A2F12 82 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4276 1591 274 4 58.0 GAGTGTTCTTTGCTGCCGTA ACATTCTTTCAAGGGCTCCA 098B1F05 AUBES2600 68 668 4 58.0 CATAAGTCAAACACCCTGTCCA GGGCAATGAATTTCCACAAA 025B2C08 AUBES3012 94 462 4 58.0 TCCCCTCTGTTTCCTGTCTG TGACAGACCCATGCATTTAATC 040A1D04 AUBES1103 185 512 4 58.0 TGGCAAAGTAGCACTGTGTC GGAGTGAGCTGTGTGCCCTG 010A2A08 AUBES2682 419 524 4 58.0 TGAAGCAGTATTCCCGACTTG TTGCCTTATAAGCAGCAGAGG 006B1E01 AUBES2403 802 484 4 58.0 TTGCTTTTGTTATACGTTGCATTT AATCTACTCCTGGCGCATTG 045A2A03 AUBES4972 877 311 4 58.0 TCTGTTAATGTCTCCAAGACCAAA CACAATGTGAGGCATCATTTACA 011B2H03 AUBES3576 939 247 4 58.0 CCACCATCCCGTACAGTTTC TGTCGCATTTGGATAAGACG 106B2G01 AUBES2078 1876 299 4 58.0 CCACATCAACATTGGACAGG GGTTGACATTTGGCCTCATA 039B2F01 AUBES5193 2120 200 4 58.0 CTTGTGTCCTTTGGCAAATTC GCCAGGAAGGGAGAAAAACT 011A1D07 AUBES5296 2644 279 4 58.0 CACTCTGTTTGACCTTGTCAGG GCAATGTGCGCTATGGATT 014A1D04 AUBES2459 singleton 4 58.0 CGAGCGTGAGGTTCTTTTTC CGGCACTTCTATGATGAGCA 040B1G12 AUBES4939 598 445 4 58.0 CAAGAGTCAACCAAATGAATGAA GGATCTGCCCAGACATCATC 098B1A10 AUBES4681 2832 159 4 58.0 TCCGAATGTTATGGCAGGA GGTCAGGAAGAAAACGATGC 108A2B06 AUBES4070 557 365 4 58.0 GGTGTCGTCCAGAAACGAG GTCCCAACTTCCTGTTTTGC 006B1G04 AUBES4071 557 365 4 58.0 GGTGTCGTCCAGAAACGAG GTCCCAACTTCCTGTTTTGC 058B1E04 AUBES3306 623 391 4 58.1 CAATCACACTGAGGAATGCAC GATCGCAAAGGCTGAATCAT 074B2H10 AUBES1926 787 171 4 58.1 CGATTTAACGCAGTGTAATGGA CACTCACCACTCAGCACAGG 036A2F12 AUBES4480 1506 245 4 58.1 CGTGTTGGGTTTCATTTTGTT GCAGCATGTCATGGCTTTAG 090A2A05 AUBES1555 2233 401 4 58.2 GGTGGATTCATTGGGTTTGA GGGGACAGCAAATTTACACTG 023A1C07 AUBES3171 9 2006 4 58.2 GGAAAAAGTGAAGCACTGTGAA CCAGCTGTCTTCGGTCAAAT 054B1D08 AUBES3694 1023 387 4 58.2 TTTGCATCATTTTGCAACTTC ACAAGAGCTGGCAAACCATC 031A1A11 AUBES4869 2233 401 4 58.2 GGTGGATTCATTGGGTTTGA GGGGACAGCAAATTTACACTG 023A1C07 AUBES1617 3092 181 4 58.3 GCGCTCCTCAGTCTCTCATT AAACTGCGTCGAGTTCCACT 026A1G03 AUBES3468 1598 433 4 58.4 TCCCAACATTCCGTAGTAGACC CAAATTGTGTGAGGGAGAACAA 006B2F03 AUBES2084 935 413 4 58.4 ACCCGGAAGAGAAGTGAACA GTGGACCCAAAAAGGTTACG 041A1B06 AUBES4906 238 244 4 58.5 ATCATGGCCATTCCAAAAGA CGAATGAATTGTCCTTGCTG 107A2A04 AUBES4881 647 266 4 58.6 GAGCGTGGAACCTACCTTGT CTGCGTCAGGACACCGATA 057A2G02 AUBES2829 623 391 4 58.8 CAGGGGCAGATATGGATGAG GCGGCAATAAAAATCTGTCAA 020B2E10 83 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3376 170 1074 4 58.9 CATGAGCTGCACACACTCG TGTCAAACCCCAATACTGAGAG 090A1H09 AUBES4543 2306 186 4 59.0 TGCGGGTTTTGTTTTTCTTC CGAACGTGCTAACCACTAAGC 086B1F03 AUBES1319 860 264 4 59.3 GTGTTTTCTGAGCCAGGAGG AGAGAAACCCTAAACTCACAGTCC 018A1E08 AUBES5388 3409 211 4 59.5 ATGGAGGCACAAAAAGCACT GCGCAAACTTTCAGTACCAC 076B1B02 AUBES5134 1640 252 4 59.5 CGACTCACGCACCCTAAGA GACCCACTATCTGCAGGGATAC 055A1E03 AUBES4971 863 266 4 59.6 TTTAGGGAAGGCCCACCTAT GTGCTGGATCACTTCCTGGT 067B2F02 AUBES4708 3120 181 4 59.6 ACAAGGCTCCGAAAGACAGA ACAGTGAGTGGGCTTTACGG 082B2F07 AUBES2505 1431 445 4 59.6 TTCCAAACACACAAACTCACC AAAGTGAGGGCGTGTCACTAA 048A2A05 AUBES4270 1431 445 4 59.6 CAAACCTGTGTGACTGTGAGC GAAAGTGAGGGCGTGTCACTA 048A2A05 AUBES4709 3120 181 4 59.7 CTCCCAAAATTCCCTTAGCC TTTTAAGCTTCCGGTTTAAGTG 093A2E02 AUBES3760 426 350 4 59.8 CAAGCAGCAAAGTCTGTGGA CCACATGGACCTTGGAACAC 061B1G06 AUBES3312 703 250 4 60.2 ACAATACGATTGGGCTGCTT CCCTGTAGCCATCAACTTGC 042B1C08 AUBES2617 442 915 4 60.8 GGGTCACTAACTCAGGTGTGG AGGTACAAAATGCCTTGACG 053B2B04 AUBES5409 3528 179 4 61.1 AGTGGCACACTGCACAAGAC TGGGAGGAAACAGTGTCAAA 074B2B01 AUBES3608 1487 546 4 61.5 TCTGCAAATCCAGTTCACCA AACCTTTTCTGGCTCTGGAA 029A1H01 AUBES3607 1487 546 4 61.7 CATGCGACCCTTTGTAGGAT TCAGCCAAAGGAAAATGTCA 041B2C01 AUBES5377 3363 235 4 61.8 TTGCAAACAGCTGAAAGTCTG CGCAAGGTCACAAATGAAGA 054B2A11 AUBES2921 1069 267 4 62.2 GGGACAGAGGACTTTTCTGCT AAGTTACAACTAATTCTGGAGGTGAA 036B2D05 AUBES3154 442 915 4 63.0 CGGACGTCACAGAACTCAAG GCTTAGACGCGCAGAGTGAT 011A1B06 AUBES5267 2524 232 4 65.3 GGCGTTACAGTTCCGAGACA TCGGTCTCCCCCTTACTTCT 003A1F11 AUBES5380 3381 194 4 66.4 TACTGCCACTTGAGCGACTG GCGTCTGAAACAGAAGCAGA 025B2A09 AUBES4498 2424 289 4 86.1 TCAGATCACGTCGCTACTGG CTCCATTGATGCAGTGAAGC 096A2D04 AUBES2508 1653 394 4 87.9 ATTGCAAGGACTGTTGGACA CACAGGTTGCAAAGTGACTGA 048A2C04 AUBES5362 3292 205 5 9.1 CTGGACGAGCTCCAGGTAAC TCGCTCACCTTGATCTATGC 070A2A08 AUBES3782 1716 580 5 15.7 CCTTGAGCTCTGGAAAGGTG TTTGTCACGTCAGCACCAGT 038A1A01 AUBES4842 1613 325 5 16.4 GTCAGACGGTGTCGGTTAGC CGTCTCCTTACAGAAAACTTCACC 074A2E04 AUBES5346 3233 183 5 19.9 CATATTTGGCGATGTTTCCA TGGGAAATTCCTCCTCTCCT 103A2A09 AUBES2883 341 651 5 22.3 GGCATCATGAGAAGCAGAGC GCCAAACTCACCACACTGTCT 054B1B08 AUBES3002 341 651 5 24.3 GGTTTGCCCACATTTTTGTT GGGCATTTGTGCTTGTAGGT 055A2G09 84 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4868 2185 271 5 29.8 GTTCATCTGCCGCGACTATT CCCCTCAGAGAAAGAGAGAGC 070A1E04 AUBES4991 1003 264 5 34.6 TGTGGCATGATTAACGTTCTG CCCTATGTCACTGTGCAACC 082A2G03 AUBES3695 1044 223 5 37.3 CTGTGCATGAGGCAGACATT GGCTCCATCCATCCAGTTAT 047A2H08 AUBES4244 1044 223 5 37.5 CTGTGCATGAGGCAGACATT ATGGCTCCATCCATCCAGTT 047A2H08 AUBES2759 338 599 5 39.1 GGTTGAACCTGAAAAGATTGGA CATGTAACACTGGCAGTTATGGA 054A2B11 AUBES1952 1110 512 5 40.6 CCCTGTTGCTACCCGTGTT GCGAACGTGCTAACCACTAA 021A1B04 AUBES1422 494 370 5 41.1 CCATAACGCTTCCAGAGTGAC GACTGCTGAATTTAAGTGAGGGAG 018B2E07 AUBES2457 1137 242 5 41.5 CGCTCCGTCTTCACTTCTTC GTTCCCTTAAGCCAACACCA 040B1G05 AUBES2559 15 539 5 41.9 ACATTTCATGCAGGAGCAGTT TAATTGCAGGGAATGGAAGG 031A2E11 AUBES4357 653 288 5 42.7 GAGTGGTCCTGAGAGGTGGA TGCTATGCTGCAAAGACAATG 001B1F04 AUBES4529 1002 238 5 43.1 CGGAGCAATACATCACTGGA TCTCCACCACTCACATGCAC 006B2B03 AUBES5089 1505 245 5 43.4 GCGAGAGAGTGTGTGCATGT AGCTGGTGAATCGTGTGTTG 072A2G07 AUBES4339 2266 171 5 44.1 AGGAAACAGGCCAGACTGAA GAAGATGGATGGATGGGAGT 008A2C10 AUBES3629 427 306 5 44.1 CCAGGCCACCTCAATATGAT AAGGAACTTACACAAACAACTTTGC 109B1E11 AUBES2557 190 529 5 44.4 CGTGTTTGTGTAAATGCGTGT ATTGCAACTGGGTGAATGTG 003A1F02 AUBES4346 190 529 5 44.5 CGTGTTTGTGTAAATGCGTGT GGGTGAATGTGCTTTATGCTC 003A1F02 AUBES3630 427 306 5 44.5 CCTGAGTTTTGGGTGCAAAG AACAAAAGCCCCTCACACAC 085A1H07 AUBES4374 1234 424 5 44.5 CTGGAGTGCGATCTGTGAAG CAGGGTTCAGTGACGTAGAGC 086A1C02 AUBES3596 1234 424 5 44.5 CTTGCAGTTTTGCAGCAATC GCTACAGCCTGCACCATTCT 112A1F10 AUBES4024 1291 279 5 44.5 ATCCAGAGGCAGTCCTGGTA TCTGGTTTCCTCCCAGTGTC 076A2A11 AUBES4574 1863 211 5 44.5 TGAATCCATGGAGCAACATC TGTGATGGGGAAAAGTGGAT 038A1A04 AUBES2993 1110 512 5 44.7 AGCATTCTTTGGATGGGTAA GCTTCAGTTTGTCCCTCTGG 082B1H09 AUBES4052 137 298 5 44.8 ATTGGCATTTTGAGCCATTC CAATGCCCAATTTGTACGAC 104B2A05 AUBES4528 1002 238 5 44.9 GACCTTGCCATTCCAACAGT TCTGTCATGTGTTGGGCTTT 002B1G04 AUBES4425 2839 245 5 45.3 GATCCATTGGAACACACGAA ATTACCTGGATGTGGCTTCC 028A1E01 AUBES4440 2839 245 5 45.6 GCATAAACGTTGGGTTCAGG GTCTGCGCAAGTGTGAATGT 002B2B02 AUBES4441 2839 245 5 45.7 GATCCATTGGAACACACGAA AATTACCTGGATGTGGCTTCC 028A1E01 AUBES3590 1090 289 5 45.7 CGCTTTGTACATGCAGTGTTG CATCCTGATTAGGGCTGTGG 042B2A06 AUBES2975 1714 504 5 45.8 CTCTGTCTTCCGGGTTTGG CAAATTCTCCTTGGCTCCTG 060A1C06 85 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3274 1135 573 5 45.8 TCTGTTGCAGCAAATCCAAG TGCAATTCCCTCATTTCAGAC 047B1G09 AUBES4406 2112 232 5 45.9 GGTTATCGGTATCGGCTGAG CAGTGTTGTCGCATGAACAG 030B2G03 AUBES4688 2864 169 5 45.9 TCAGCAGGTTCTCCCATTTC GGCTGCTGGGAACTGTAGTC 093A2D02 AUBES2478 534 1072 5 45.9 CAGCCTGAGGAGACTTGTGA GGGCACAGCAAAGATTGTATT 046A1E08 AUBES2005 3037 306 5 46.0 CAGCGAGTCTTGCCTCATTT TGTGGCTGAATTCTGCTGAT 029A1G07 AUBES4700 3037 306 5 46.0 CCTCATTTTCCCTCATGTCG TGTGGCTGAATTCTGCTGAT 058B1F01 AUBES4331 2182 210 5 46.0 AGTGGTCCTATGGGGTTCCT GCACTGCAGCTGGAAATACC 078B1E03 AUBES2438 1742 244 5 46.0 TGTTTTTGGGAGGTGAGAGG CCCTCTGCAAGCCATGATTA 040A2E04 AUBES4424 2839 245 5 46.1 GCATAAACGTTGGGTTCAGG GTCTGCGCAAGTGTGAATGT 002B2B02 AUBES3117 495 866 5 46.3 TACACTGGCAGATGCCTTCA TGTGTTTCTTGCATGACGTGT 036A1G09 AUBES4929 527 309 5 46.4 GACCCGTACTGAATTGAATGG AGAAATGGAGCGAGCACTGT 028B2D05 AUBES4897 54 364 5 46.4 GCATGACTGTGCTGGCTAGA CAGGTGGTTAGTGGCAGGTT 035B1D06 AUBES2638 534 1072 5 46.4 CTGCTTGGACTGTGTTGCAT GCACTCCAGCCAGCTTAGTC 023B2H03 AUBES3446 853 403 5 46.4 GCACACTCCTACCAGCCCTA GTAATGCAACGCAATGTTCG 106B1B02 AUBES5076 1426 184 5 46.5 TTTGAAATGCAGCTGGTTAAA CCAAAGAAGCCGTGATGTTT 007A2B05 AUBES3237 534 1072 5 46.7 GTGCAGATCCACATAAGTCCA GGATAAACCCGTGTTGTGGT 023B2G10 AUBES3445 853 403 5 46.8 CCTGGAATACTGGGCTCAAA CGATAAGCAAATGACAGTACAACA 023A1A02 AUBES4035 1402 274 5 47.1 CCACTCTGACATTCCCACAG TGGGTTTGGTTTGACCAGAG 058A2F06 AUBES3170 534 1072 5 47.4 CCCATTGCCAGTATTGGTATC CGGTGACCCTGTGTAGGATAA 086B1B08 AUBES1703 413 381 5 47.9 TCCAAGCTCACATTCAGCAC GCCCCAATTCACTAACAACC 033A2F02 AUBES3402 339 348 5 48.8 CTCGGTCAGCTGATGGGTAG TTCTCCCAATCTGACGTGCT 096A1E09 AUBES4349 339 348 5 48.8 CTCGGTCAGCTGATGGGTAG TTCTCCCAATCTGACGTGCT 096B1B05 AUBES5218 2269 345 5 52.3 TGCAGGAGTTCTTCCAGGAG CCTTGGGCAAGACACTGAAC 109B1D08 AUBES3595 1209 470 5 53.0 AACACCCGACTCTCCATCAG GCACTGGGCTACCTACTTGC 013B2B01 AUBES4699 3019 262 5 53.6 TTTAGTGGCTGGGAGGTTTG CTCATTCACAGTGTTGCACGTA 077B2D05 AUBES3594 1209 470 5 54.3 CCACCAATAGGGCAAGTCTG CTCCGAATCTGGTGACGATT 044B1E02 AUBES2269 319 423 5 57.8 CCGACATACACGACTTTTGC GGGGACAGCAAATTTACACTG 043A2F09 AUBES4567 1786 196 5 58.4 ACCTGCAGGATTCAGGAGTG AGGGGGTGAACTTTCAGTGAC 054A1B03 AUBES5039 1269 480 5 59.0 TATGCTTGCCTGCATCTTTG GCACAACATGTCTTTCTTGGA 070A1C06 86 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3878 913 424 5 59.5 CCATGGAGCTCTGGATCTGT CCGTTTTAACATTGCAAGACA 062A2C10 AUBES4685 2861 166 5 60.1 TGAATCACATGTAAATGAATCATACG GCAATTTCCAAACAACAGAAGG 014A2F11 AUBES2942 226 377 5 60.3 CATGGAGGGCAAATGATAGG ATGTGACCCTCATCCATCAC 023A2F09 AUBES1265 singleton 5 60.8 TGTACGTTGGCTCGTTGACC ACACACGGAGGGGTCAGAGG 033A2D11 AUBES4938 587 381 5 62.0 TTGACGTGTCCAAAGTGTCC TGGCTGTGTCTATGGTGTCAA 085A1A07 AUBES4686 2861 166 5 62.1 TTCTTGGGAAGAGCTGGAGA TGAAGAGTCCCCAAAACAGG 065A2C09 AUBES1630 3093 288 5 63.0 GAGCGCGAAGGTAAGAGTTG TTCCCTGTCTGAGCGAGTCT 032A1H08 AUBES4774 2814 218 5 63.4 TGAGAGAGACGGAGGGAGAA AGATGGAGCTGCCAGAACAC 042B2E06 AUBES2982 95 365 5 63.8 TCACTGGCATTGTTTTCATCA GCCACAGTACCCTCCACATC 108B1C03 AUBES4127 961 250 5 64.0 GGGGACTTCTTATGGCCTTC AAATAGTGAGATAATCACCTGAACAC 023B1H07 AUBES4644 520 161 5 64.6 ACCTGCTGCTGTCTGTGTCA TCTGCTGGACTGGAGAATCC 047A1H06 AUBES2814 268 309 5 65.9 AACTGACGGAAGCAATGTCC CAGGTTCAAAATGAGAGGGAAC 012A2D08 AUBES2182 961 250 5 67.6 CACACTACAGTGGAGCGACAG TTAAAAGGGGGCAGAAGGTC 027A2A08 AUBES4341 2277 247 5 67.6 ACCTCCCTCCCTGACGTAAC ATGGCAACCAGCTGAAAAAC 006A2B04 AUBES1752 1339 403 5 68.5 GGGAGTACAGTAAACCACTTGC CTCTTCCCCACCTCCAAGTA 018A1A09 AUBES3940 95 365 5 70.0 TCTGGAACACTCTGCCATACA ATGTCCGAGGACTCATTTGC 019A1F04 AUBES1144 2380 238 6 0.0 TCACAGCAACTTGCCAAAGAG ACATCAGCAAAGGCTTGACAG 007A2D08 AUBES5284 2599 223 6 3.0 TTATGGCGTTCTGGGGATTA GTTGAACCAGTGTGCCGTTT 079B1G03 AUBES4993 1020 357 6 6.5 CATCTGTGCAGTCTGATGGAA CCGTACGCTACATCCACCTT 053B1E03 AUBES1616 singleton 6 8.2 ATGGATCTGGCACAATGGAT TGGATCAAAGTCCCCAATTC 026A1G06 AUBES4389 1842 218 6 8.4 GGCTGCACATTCTAACAAGG GATGCCATCCAGTGCATTTA 031B2D11 AUBES3799 974 260 6 17.2 CAGGAACTTGTATTGCGGAAG TGTTACCACCCCGGAAATAA 020A2D03 AUBES3798 974 260 6 22.0 ACCAAGCCAACAGAGCAAAT TGATGTCGCGGTGATTATTG 002B1F04 AUBES3804 1058 367 6 25.1 CCTGGGTTTAGACTGGAGGA TAAATCTGCGCTCGGCTAAT 046B1D06 AUBES3806 1058 367 6 25.1 TGTGACCCTTTTAGAACGATG AAAAACAAACCCAACAAGCA 102A2D06 AUBES3805 1058 367 6 26.0 TTTTCTCCCACCTGCTGAAG AGTGAGTGAGTGGGCTTCGT 071B2E09 AUBES2492 singleton 6 29.7 TGCGTTTTGCAACACAGTAAC GTGCCACTTTTCAAGGGTTT 046B2A07 AUBES2343 215 249 6 32.1 CGCACCCCTCTTTACAGAAA TTTGAAAGAGCAGCCACTGT 047A1D11 AUBES1884 80 311 6 35.9 CCCAGAAAAGGGATCTTGGT CCCGTGACCCTGTACGATAA 028B1H01 87 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3004 530 489 6 37.8 ATTTTGTTGACACGCCCAGT GGGTTTAGGCAGCAAAAGAC 074A1A10 AUBES3793 786 240 6 38.1 TTCAGGATGGAGGACTTTGC TTGTGGATTCCCCAGAAGTT 074A1G05 AUBES3777 1369 326 6 38.4 CCTGAAATTCCCAGGTAATGA GCACCAACAACCATGCTACA 023A2H09 AUBES2689 766 506 6 38.7 AGAAATCCAGCCACGGAATA TGCCAGCTTTAAGCCTCTGT 011B1H02 AUBES4144 1182 303 6 39.0 GGATATCTTTGAGCAGCAGGA GAATTGCCGAACAAATCTGG 101A1B11 AUBES3934 1362 232 6 39.1 TCAGGCTGCCTTTGTTTTATG GCATGATGAAAAAGTGGGTTG 026A1F04 AUBES4108 1369 326 6 39.1 ATCCCGCAGACACAGAGAAT CTACAGACATGCCGGTCTCA 078A1C02 AUBES5235 2376 462 6 39.4 CAAGCACGTGTCCACAAACT GCTGAAATTGGTGAAACTGACA 109B1B05 AUBES4143 1182 303 6 39.6 TTGGTTTCAAGCCGCTTAGT AGAACAGATGGGGTGGTCCT 094A2A04 AUBES3812 1468 411 6 40.0 GCCTGCTGAGGGATATTCAC GGCAAATGGGAAGTGAGAAA 027B1A08 AUBES4003 1098 282 6 40.2 TGACCTTTTCATTTGTGATGATG CTCCTGAGCCTTGACTCCTG 075A2B05 AUBES3397 229 316 6 40.2 GAGGCCACTGCTTAGTGACG CACGGAATTCCAGCATTCTT 020A2H12 AUBES2805 93 205 6 40.3 TCCCCACTGTAAGAACCATGA TCTTCCGGGTTGGTGTTATG 058A2C03 AUBES2642 64 306 6 40.3 AGCATCACGGAATTCAGCA TCACAGGCTTCTCTCCGATA 008B2E09 AUBES4637 362 215 6 40.4 AACAGGAGGTTCCGAAATATCA GACGGGGGATCAATAACAGA 024B2D09 AUBES3690 776 455 6 40.4 GTCCCCAGCAAGTGAAACAT ACACATCCACGCCCTAGAGA 096A2F06 AUBES3791 645 445 6 40.4 TGCATTGATTCACTGTGGTTT ATCCACACACCGCCTGTAA 070A2B08 AUBES3774 1109 389 6 40.4 TGATCATGGCAGCTTCTGAG CTGTGCACAAATCATTCATGG 035A1B03 AUBES3809 1310 240 6 40.4 CGAATAAGTAACATTTTACACGTATGG TTGGTTGGTGAGTTTCTACCTG 109A1E10 AUBES2034 1264 424 6 40.6 CATGTGAGTGAGATGGGCTCT GCCTGGAGCTTGTGTTTGTA 038B1E03 AUBES3701 1894 330 6 40.6 AGGTGTGAGGTGTGAACGTG TCTGTCCATTCCCCAGAGAC 052B2C07 AUBES3359 29 847 6 40.8 AGAAGGGCAATTTGTGCAAT CAGCAGACCTGTTTGGAGGT 088B1C05 AUBES3423 470 360 6 40.8 TCGCACTCTCATAAGGACCA CACTCAGCCCCAGTCCTAAC 081A2C11 AUBES4802 3026 225 6 40.8 GCGAGAGGGAATAAATGCAG GTTTGAGCAAAGCCTGATGG 085B1F06 AUBES3514 171 526 6 40.8 TGGCCAGGCATAGTGAAGTA GCTTCTGCATGCAATCATGT 078B1A04 AUBES3017 502 494 6 40.8 TGTGTGGTTTCCCTCTCTCC TGCCTTTTCAGGGTGTAAGC 041B2G02 AUBES5370 3321 88 6 40.8 TCAGGGACTTCCACTCAAAAG AGATTCTTGGACCCCACTGT 101A2D01 AUBES3794 786 240 6 40.8 TTCAGGATGGAGGACTTTGC TGTTGTGGATTCCCCAGAAG 074B1G05 AUBES2811 196 276 6 40.8 ATGCGGCATTTCTCATGTCT CCCACCATTCAGAAGCTGAT 006B2A07 88 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3282 196 276 6 40.8 CCGTTTTTCTAAGAGGCTGCT TCAAAGGTTATTGCGTTGCT 098A2B12 AUBES2734 466 460 6 40.8 GGCGCGTATAAATCACCAGT CCACTGCACCCATTAGAGTG 026A1G01 AUBES3307 637 259 6 40.8 CCGAGCAAAATTGTGTATGC AGCAGGGGGAAGTTTCTTTC 087B2G01 AUBES4963 807 267 6 40.8 CCGGTCTAAAATATTTAATGGTGA GGCTGTTTTAAGGAAAAATTCAGA 042B1B04 AUBES3692 830 675 6 40.8 GAGGGCCGTTGGATTACTTT CTTGCAAACGGTCCATAACA 095B2D08 AUBES4371 1090 289 6 40.8 GAGGCGTGTGTGGTTATGTTT TCCCAACAGTCAGTGATTCAG 101A1E06 AUBES1306 766 506 6 40.8 CACTCCCGGAATACACGCAG TCAGCTCCAGCGCAACGAGG 019B2H05 AUBES3773 1059 450 6 41.0 AGCCAACAATTTCCATGAGG TTCATGTCAGCGGTTCACTC 020A1H08 AUBES3955 530 489 6 41.0 TTCTGTGCACACGATACCATC TCACATTGCACAGATGCTCA 014B2B08 AUBES3772 994 340 6 41.0 GGGCACTAATGAGCACACAA TGTTAAGCCTCAGCCTTTCAT 066A1A07 AUBES3795 808 171 6 41.0 TGACCTCAACAATGTATGCTCA TTCTGACATGCCGATCTTTT 073B1E11 AUBES3900 2630 205 6 41.0 AGCAGCAGCACAATGCATAA TCCATAATCCCCCATTTCTTC 100B1B06 AUBES3892 1455 338 6 41.0 TGATAGAACTGGAACACACAAATG CTCAAAAGCAGGATCCGAGA 106A1A08 AUBES4707 3110 191 6 41.0 TTATTTTTGCCAGGGGTTGT TTCTCACCACTTGGGGTCTG 070B1H10 AUBES3434 590 381 6 41.1 AGCCGCAGTGTATCCAGACT CTGCTTCATCGCTCTGTCAA 098A2H11 AUBES3792 786 240 6 41.1 TAACTCCGCGTTCCTAAAGC GATGCTGGTGGTAGAGAGTGC 045B2D04 AUBES3458 1031 259 6 41.1 TGGTGTCCCAGATAGGGTGT CTTAAACCCCTGGACCCACT 109B1C05 AUBES3614 1635 411 6 41.1 GGTCGCTAATACCCTCATCG CTGGAAAATCACGAATTGGA 111A2A03 AUBES3613 1635 411 6 41.1 ACGGCAAAAGGAAACTGATG GCCAGCTAACGCATTCTCAC 096A1A01 AUBES3852 3026 225 6 41.1 GCGAGAGGGAATAAATGCAG GTTTGAGCAAAGCCTGATGG 085B1F06 AUBES2606 29 847 6 41.1 GATCTTTTTCGGTCTCCCATC GTCTGCCAACAGGAGTGTCA 060B2H03 AUBES2623 37 572 6 41.1 ACCTAGCGTGGATTCAGCAC CTGCTTCCGTCCACTCCTT 038B2C08 AUBES3155 193 868 6 41.1 AAGGCTGGCTCAGAAAAGTG CTACCAGCTTGCTCCTCTGC 072B2F02 AUBES2602 278 511 6 41.1 GCGCAATGACAGCTTAAACA TTACACCTGGCGGCATATTT 020B2B02 AUBES3759 374 244 6 41.1 AACCAGCGGAGTTTGTGTCT CGATCGTCTGTCATCCATTG 087B1H04 AUBES3789 645 445 6 41.1 TCCCCTGTGAGACAGAAAGG GCCCAAAAGTGGATTATCAAA 018B1G04 AUBES3457 1031 259 6 41.1 TGAACACCAGGACAACATGAA GGTAACCACTACGCCACCAT 072A1G08 AUBES2062 1098 282 6 41.1 CCTATCCTCAAGGCAAGCAT GCAAGAATCTGCAAAAGAAGC 039A2G08 AUBES1935 466 460 6 41.1 AGCCATGACACCTGCTGATT TGTTGCCAGCACCTATCATC 036A1D01 89 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3766 552 424 6 41.1 CGCTTGCCAGCCTAAATTAT CACTCGAATAGCGAATAGTTGG 078A1A02 AUBES3698 1455 338 6 41.1 CATGATAGAACTGGAACACACAAA CTCAAAAGCAGGATCCGAGA 106A1A08 AUBES3845 2533 159 6 41.1 CATTTCGATCCAGGGTGTTT CAAATCTGTCGGGCTTGACT 111B2D04 AUBES1793 singleton 6 41.1 TTCACCCTATCAGCCCATTC CAGAGAGAAGCTTTTTGGTGTG 034B1G12 AUBES3899 2630 205 6 41.1 CTGTGCAGCCCAACATGA GGGACAGAGAGGCAGAGAGA 068A2D11 AUBES3255 346 457 6 41.1 GGCCTTGCATTTGAGAATTT GGCAAACACAAGTGAAGCTG 070A1H08 AUBES2195 1182 303 6 41.1 GTCTCCCCCACCTTATCCTG GGGGGTTTAGTTGCAAAAGG 028B1C10 AUBES2227 196 276 6 41.1 AGGCTGAGAGTCTGTGGTCAG TCTCTGGCTTCCCTCGTTTA 042A1C09 AUBES2952 555 321 6 41.3 TCCAGTCGTGTTGACACAGG ACACCCACACAATGCACATC 003B2E04 AUBES3015 769 485 6 41.3 TTCTGTTGGTGGAAAGATCTGA TGACCATAATCCAACTCTTCAAT 040B2G07 AUBES3824 1816 279 6 41.3 TTTTTGGAGTAGCTTCTTAGGATATGA TTCCACCCAAATGAGACACA 013B2B04 AUBES2729 8 279 6 41.3 AGATGAGACAGGTAAGCAAGTACA AGCTCAAAGCAGCTCCAGTC 018B1D06 AUBES3681 171 526 6 41.3 TGAAAACACAAATGCATCCAA AGGCAAACAAGGATGTAGCAC 071A2C10 AUBES3016 466 460 6 41.3 CCATGGCTCCTGGATTTAAG CCAGCCAGCAAACATGAATA 041A1D03 AUBES3664 590 381 6 41.3 GGATGGACGCACACAGATAA AAGGGCGTGTGTAAAGTTCG 051A2C05 AUBES4049 1522 254 6 41.3 CCATGCTGACATCACAGGAG TCAAAGCAGTCGGTCTGATG 038A2D07 AUBES3323 1776 573 6 41.3 TGCCTACCTGCAGTATTTTCAA CAGTTTGCAGGACAAGAACG 090A1C05 AUBES4782 2880 178 6 41.3 TTGGACAAAAGTCCTTCATCG TGTCATGATCTCTGACCACCA 007A2H06 AUBES5342 3216 161 6 41.3 ACGGCCGGCTTTAATCTAAT GACATCGTAGCGCACAACAG 039B2F12 AUBES3309 666 353 6 41.3 TTCCAAAGGATTGCAAGGAG CAGGCTAACTGGGGACACTG 084B1A10 AUBES4304 1885 249 6 41.3 TCCTGACTGCCAACAGAGATT CCACCATGCATTTTGTTTTG 027B2C02 AUBES3846 2654 369 6 41.3 CATTGCCAGAACCTCACAGA GCAATTGTGCATTGATCAGC 042B2D03 AUBES1185 37 572 6 41.8 TGTTTACCGGCTGTGCGAGC TCATGCCATTTAGCGGCCTG 011B1H04 AUBES4247 1127 347 6 41.8 CAGTCATGTCAGGAGGGTGA CTTTGATGTCCAATGCACCA 011A2C06 AUBES3844 2533 159 6 41.8 GGATAAGGACGTCTGCCAAC GGTGCAAGGAAAATGACAGG 024A1A04 AUBES3398 229 316 6 41.8 CACGGAATTCCAGCATTCTT TGTAGTTTGAGGCCACAGCTT 060B1H01 AUBES3954 530 489 6 41.9 AATGGCATCTTCTCCATGCT GGATGCATTTGCACAGTGTC 003A1C12 AUBES2779 776 455 6 41.9 CCAAAAATTGCCAAAGCATC TGTCCTACACTGGCACCTTG 006B2B08 AUBES3097 193 868 6 41.9 AAGCATTGTGAGTCCCTAGCTC TGCATTGCTCAACTCTAATACAC 023A2A01 90 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3384 278 511 6 42.0 CGAATTGCACCTACAGAGATGA CGTGCACCTGAAACGTAATTT 072B2B09 AUBES1564 singleton 6 42.4 GCCTCGTAGAAACCAAGGTG TGCACGTTGTTCCTTTCATT 021A1A06 AUBES3355 278 511 6 42.8 GGATGTGTAAAGCCCCAGTG ACTGGTGCTCCCAACTTCAA 078A1A09 AUBES3280 93 205 6 43.0 CCCCACTGTAAGAACCATGA ACGTAAGAGCTCTGGCCACT 083A2G05 AUBES3591 1090 289 6 43.1 CAAATCTGGGCCAAATGAAG GAGGAGTTGCCTCAGGAAGA 050B1B01 AUBES3683 297 252 6 43.5 TGGATGACCTCCTCCTTCTC GCGGCATTTTTCAACCTAAA 046A1F10 AUBES3433 590 381 6 43.7 GTGGGGGAGAAAATCCATTC ACAAGCACACATTCCCTCGT 026A1A03 AUBES3823 1802 244 6 43.9 GCTCGATATGACTGACTGGATG TTTCAGGAACAGCTGAAAAATG 026A2D06 AUBES1282 2833 352 6 44.1 TCATTCTGCCACCTGCTGAC CTGTGACTCCCTCAGACTGC 002A1H01 AUBES3103 370 891 6 44.2 GTGTTCAGATGGTGGTCCAA GTTTGAAGGCAACTGCACCT 093A2C08 AUBES3831 1881 316 6 44.7 GGTGGAGACTGAGAGGCAAG CACCTGAGGCAAACACCTCT 003B2D11 AUBES2987 346 457 6 45.8 TTCTCGTCAGAAACCTTTGGA TGTTTCTGAGATTGGCTCTGG 090A2A03 AUBES5151 1710 254 6 48.0 CATGAACCTCCACTGACGTG CCTATGGGTCAAAGGTCAGC 060A2B06 AUBES3784 1894 330 6 54.0 AGGTGTGAGGTGTGAACGTG TCTGTCCATTCCCCAGAGAC 052B2C07 AUBES2386 560 675 6 71.6 GGCGCCTTCAATAAATCAAA TACCGGTATATCGCAAACGA 044B1G11 AUBES2070 singleton 7 39.2 TTGCAGGTGTGACTGGATTT TCCACTCAGATTCCTTGCAG 039B1F05 AUBES3963 1528 281 7 41.0 TCTGTGAATGATCCCCATCC TACAGACTCGATCCCCTTCG 053A2F07 AUBES5117 1540 252 7 45.1 AAAGAAGGTTGCTCCTGCAC GTAATGTGTGGCGCGTATGT 042B1D08 AUBES5085 1476 206 7 49.6 TGGCACCCTTGGTAAGTATG CACCACATTTCAGGTGGAACT 111A1B08 AUBES2897 608 490 7 53.4 CCCCTTTTGTGTGGGATTTA CCGAAGGGCACTAATTAGGAT 052A1E06 AUBES3408 369 492 7 54.3 CAGAAATATGCGTGCAAACC GCGCCCTAAATATGGGAGAT 008B2C12 AUBES2898 608 490 7 55.4 CCCATCCAGGGTTCTTGAAT TGAATTACCTGAAGGTGGCATT 074B1D07 AUBES3975 1731 227 7 57.2 CAATGTCTCATTTGCACAGC TTTGGTCAAGTCAAAATGATGAG 077A1E08 AUBES2995 1461 409 7 58.5 CCCTCCTCTGCCCTTTTAAT CCTCCCCCATACACACAAAC 107B2E02 AUBES4461 805 194 7 58.7 AGATGTAGCGCATCCACCTC GGTCCTGTAAGTAGGGACCAGT 108A1B09 AUBES4638 380 200 7 59.1 GCTTGGACCCCTAACAACAA GTGTTTGTGGCTGTTCCAGA 029B2F11 AUBES3409 369 492 7 61.7 CACCTGAGGTCAAATCACCA GTGCGTTTGGGCTATAAAGTG 078A2H10 AUBES2592 216 727 7 64.7 GGAGCATAATCCCAGAGCAC TGTTCACCTTTATACTTCCATCTTATT 014B1D07 AUBES3136 216 727 7 64.7 GCCCATGCCTAAGAGGATTA CCACAGCCCTGTGTAGGATA 107B1G02 91 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2401 452 330 7 65.6 GCAAGCACGTGACTGATTTG TGACCTTGCTTTCTCTTCATCTC 045A1H07 AUBES3216 404 868 7 66.0 GTGAGGCGGAAATGCTACTT AATGCCTTGACAGCATGTTC 047A2G05 AUBES4668 963 392 7 66.4 TTAAGATGTGGCAACGGATG AAGCACATAATCTCAGTTCACACC 020B2H09 AUBES2639 9 2006 7 66.6 AACCCTCACTAATGGCTAATGC TAGCAGCAGTGGTTGAATGG 021B2C03 AUBES5416 3545 173 7 67.0 TTGTGTCTTGTGGGCTATGTG AAGGTGGGTGAAAGAGTAGTTACAG 057B1E05 AUBES2799 1085 347 7 67.3 GCGGTACTGTACTTCCATCCA GATGACCTTCCATGCCTGTC 029B2A11 AUBES4243 1014 408 7 67.7 GATCCGCTCTTAGACACGAGA TTGACAGTGCGGTAAGATGC 025B2G11 AUBES5040 1271 245 7 68.0 CTGCGCTTTCATCTTCTCTG ACGTTTCAGTCGAGTGTCCA 059B1E02 AUBES1744 1428 227 7 68.3 TTTGATGCTGCTTTCACCTG CATCCTTCCTTTCCTTCACG 020A1F05 AUBES3056 275 822 7 68.7 CATGAAAGCAGGAAAGTCAGG CAAGCAAACATCAGGCTCTTC 038B2B05 AUBES3086 726 746 7 68.7 ACCCTGTGCAGGATAAGCAG GCCAGCTTGTCTCACATCAA 046A2E09 AUBES5144 1687 198 7 68.7 GCAGGCTTGTGAGGTTAGTGT CAGGATTAACCATGATACCAGGA 100B2H04 AUBES4134 986 254 7 68.9 CAGAGACCAGCACAAAGACG CAGAGGCAGACCCATAACCA 011A1A05 AUBES5114 1531 538 7 68.9 TTGAAGCTTGAAATTAGTCAAAAGAA GCATGTTTCCAGGGAGAAAAA 006A1D01 AUBES3284 223 320 7 69.0 AGGTGAAATTCGGCACAATC TCCCTTTCTGCATTTTAGACC 083A1E07 AUBES3487 429 301 7 69.0 TCTCCCTCTTGGACATCTGC GCAGTCCAAGGACAAACCAT 083A2E10 AUBES2318 690 524 7 69.0 CTGTCAATGTGTGGCTCACC GGCAACCATCTGTGTTTGGT 026B2C07 AUBES3488 429 301 7 69.0 CTGAGGATGGTGGTTTTTGC AAGTGGCGGTGGATATGGT 040A2H11 AUBES4338 2245 291 7 69.0 AGGCCCAGTTGGTATTGGAT CAATGATTCTCATCCCCAAA 055B2F03 AUBES4122 83 223 7 69.0 GCTTTACATTGACCTCGCTTG TGCCCTTACTCACTGAAGTGTC 078B1G02 AUBES4550 2951 232 7 69.2 AGGCCAGATGCCTACCATAG CCAGTGCACCAGTGTTATCTG 010A1F01 AUBES3471 57 245 7 69.2 TCCCCGTGAAGTCTGTGATA GCAAGCTTGGCTTGTTGATT 082A1A12 AUBES5200 2145 220 7 69.4 TCTTTGCCTAAGCCCTTGAC CTGTGATTTGCCTAGCAACTGT 004B2F07 AUBES2951 531 281 7 69.4 TGCTACAGCTGTGAGGTCCA GTTGGGGAATTGGGTGAGAG 026A2D08 AUBES4667 963 392 7 69.6 CCATGGCTGCCAAATGTATAG GTGACCTGGGGAAATGTTCA 006A1C07 AUBES3248 152 357 7 69.6 TTGCTGTGATAAATGTGCGTAGT CAAATGAGCAGGGAATAAAACA 109A2B04 AUBES3744 9 2006 7 69.7 TGAGCCATGCTCTGGATACTT TCAGAGCAACTCCTGAAGCA 088B2D11 AUBES2672 1018 304 7 69.7 ACATTGCCGTATCCACACAG AATGCCTGATGAGGTTGGAG 053A2H09 AUBES4719 3251 164 7 69.7 GCCAAAACAGCAATAAGGTGA TCTGAGGTTGAGGAACTGTAGGA 021A2A03 92 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3969 1657 279 7 69.7 GCAGGGAGCATGTTGCTAA TCGCTACAGCTCAGAACGAA 112B2F07 AUBES1805 328 392 7 69.7 ACAGTGCTGGTGATGCTGTC AGCCTTCAGAGCCATGCTAC 033B2G02 AUBES3703 9 2006 7 69.7 GGTTCCGAGGTTGAAGATTG CATGCCCACAAGTTTCCTTT 005B2G07 AUBES3400 267 364 7 69.8 CTAGCAGCACAATTGGCATT TTCCCTGTGACCCTGAAAAG 067B2H09 AUBES3399 267 364 7 69.8 TTGTCCCGACCTTACCATTC ATGAGCAAGCGGTAATGGAG 010B2E12 AUBES3718 328 392 7 69.8 GGACATTTGGTCCCATCAAG CACGAGCTGATTTGCAAGAG 035B2E01 AUBES1809 963 392 7 69.8 TCATGTCATCCGGTTTGTGT CGAATGATCCAAAGCCCTAA 033B2D06 AUBES3660 400 257 7 69.9 TACTAAGGAGCCCTGCGATG CATGAATGTGTGGTTCTGTGG 034B2E02 AUBES2751 162 492 7 70.0 TCACACCACAGAACTGACAGC CAAGCATGCCTATGTTTGTAGG 038A1E05 AUBES4097 1016 404 7 70.1 GCCACTTTCTGATGGGACAT TGTCATGCACAATGTGTCAACT 034B2B11 AUBES4098 1016 404 7 70.4 TGAACTGAACCCCCAGAGAG TGAACAGGGAGCTGAACTGA 052A1H01 AUBES2504 825 340 7 70.5 TATGAGCGTGTGGTGGGTTT CACAATGAACTGCCCTTTTG 048A1H02 AUBES4135 986 254 7 70.7 TTTGTCTTGGTCGTCTTGGTC CCAGTGTTAAAGACAATGTTGACG 076A2H10 AUBES5149 1707 235 7 70.8 TTTGTGCAAAATGAAACTTGCT CTGAGATCTGGGGAATTTGG 024B1E03 AUBES2925 1120 452 7 71.7 TGAGTGCATTTTGCAGTTGG ATCACCAGGTAATGCCGAAG 031A1C12 AUBES4348 267 364 7 71.8 GCAGCACAATTGGCATTATT TTCCCTGTGACCCTGAAAAG 067B2H09 AUBES2769 488 636 7 72.2 CCCGTTCAGAGATGTTACGC GCTGGAAAGATTCAATGTCCA 072B2E03 AUBES2905 690 524 7 73.3 GTCATGTGTGGCTCCCAAGT GAGGGGGAAGAGGAAAACAT 053B1E10 AUBES2692 410 450 7 74.2 ATGCACCCGAGGTTTTAATG ACCCCTTCATGACAACGGTA 012A1G11 AUBES2562 275 822 7 78.3 ATTAGTGGTCTGGGCGCTTT GAACAGCAAAATGTCACCAAA 050B1H02 AUBES4813 3091 205 8 0.0 TGCCCCCAATGTCTGAATAC TCTCGTCTTCCTGGTGCTCT 104A1H02 AUBES3538 181 695 8 4.0 CAATTGGGCGTTTTATTTGG ATGAAGCGTTGGATGGATTC 090A1H01 AUBES4368 895 359 8 6.1 GACACCCAGTCAGTTGTGGA CAGTGTTTGGTAGGGTTGCAT 109B1A01 AUBES4367 895 359 8 6.2 CCTCTGTGGCTTTTCAAAGAA AAATCCATGTTAGCGGCTTT 074A1D08 AUBES3573 895 359 8 6.2 GACACCCAGTCAGTTGTGGA CAGTGTTTGGTAGGGTTGCAT 027B2G08 AUBES3574 895 359 8 6.2 TGTTTGTCCCGGTACTATGTTG CCTTCATGAACCATGATCTGC 008A1F10 AUBES3328 480 709 8 10.1 AAGGAGTCTCCAGTGTCAGCA TGAGGAACATCCAAGAGTTTCA 081A2B06 AUBES3357 455 727 8 10.5 TGGCTGAGGAGCTAGAGCAT GAAGTCCCATCACCAGCAAT 059B1C02 AUBES2576 206 658 8 10.6 CATGGTCTGAGCCTGAAGGT ATATTGCCCAGCCCTAATCC 038B1B08 93 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3144 455 727 8 10.7 TGGCTGAGGAGCTAGAGCAT GAAGTCCCATCACCAGCAAT 093A2F06 AUBES4472 1270 238 8 13.5 TCAGGACGGTTCGGAAACTA AAGCAACGGTGGGAGAATTA 107A1C07 AUBES5275 2555 169 8 15.5 CCCCCTTCACATTCACCATA CACATCCGCTGACTCACTGT 011A2D09 AUBES1932 singleton 8 15.8 TGTCATTGAGAAACCCTCCA TTGCTGGTGTTAGATGTGGTG 036A1A09 AUBES4546 2810 352 8 16.8 GCCTAAGAGACTGGCTGTGG CGGAGAAATCATGTCCTGGT 073B2G07 AUBES3316 774 381 8 17.6 TTCAACATTAAAGCCTACACCTG TTCACCTCCGGAATATGGTT 085B2H11 AUBES5328 2982 228 8 20.6 AGCTTATTTGTAGGTGGTAATAGTAGG CGAGCATAACTGCTTTGCTACA 010B2B01 AUBES2722 506 533 8 25.1 GGTGGCGACGTGATCTCTAC CGCTGTCTTTTCTCCTGTGG 012A1H06 AUBES3380 189 622 8 28.9 CTGACATGTTTCTGATGGATACAA CGCATCTGCATTACACAAAC 095B2D07 AUBES3203 189 622 8 30.6 GCTGGGTACTCTGGTTACACAA AACAGCTTTAACCCCGATGA 057A2F09 AUBES3326 189 622 8 32.3 GTTAGAGGCCAAGGCGAAAT GGATTTGCATGTCCAAAATG 092B2B07 AUBES1354 378 276 8 34.4 ACACTGAAGCGGAACGATGG ACAACGCTCAGTTGCTGGAC 032A2H07 AUBES4086 812 470 8 36.7 CCTGGTTTTAAAAGCGCAAC TCATTACAACGCCATCCTGA 088A1E08 AUBES1353 2128 205 8 37.5 GTCCTCCTGAAGTCCAGACG TCACCAGCTTGCTCTGAGAC 021B2H09 AUBES1990 2128 205 8 38.0 TGATGTAGAAGACCCCCATGT TGCAGCTCCTAGTGGTATGC 025B2E12 AUBES5277 2559 208 8 38.4 GCTAATCCCTCACAGGCAAG GATTTCCAGAAAGGCTCCATC 088B1B05 AUBES5036 1265 203 8 38.5 GGCGCAGTCACAAAGATGTA GCGAGATGCTGATGGTACTG 013B1C07 AUBES4126 905 370 8 38.9 TCTGCTTTGTTGGTGGTAAGG TGCCACAAAAACACATCCAT 107A2G07 AUBES4610 2054 240 8 39.2 CGTCTCTCACCCATCAGCTC ACATCATCGATTCGCTGTAGA 064A2H12 AUBES2532 1056 210 8 41.0 TCGGATTCCTCATCATCCAC ACGGCCAAAGAAACAAACAA 049A1E10 AUBES5306 2693 122 8 41.4 CTGTGAGTGGAAAGTGAAGCA TATGGGCAGTAGGTGGCACT 021B1G05 AUBES4302 1845 238 8 41.9 TCGGTTAACATGCATCCGTA GAGCTGACAGAGGGGAAAGG 005B1D04 AUBES3747 375 401 8 42.4 AGAAGGGAGTGCAGAAGACG CTCAGACCTGGAGGCCAGTA 102A1B06 AUBES3365 885 1133 8 42.8 TCAAATGATGCCCAGGAAAT GGCCTGCCAGAATCTACTGA 074A2E10 AUBES1113 201 330 8 42.8 GCAAAGTGTCATTTCTTCAGTGTCG GCCATGTTCAGGTAACGTGG 012B2G07 AUBES4229 743 259 8 42.8 GACTGCATCTGCACCAGCTA TCTCGATACTTTCTCTTTTGCTCTC 023A1C08 AUBES4427 2866 193 8 43.1 TGCATGTTTACCATTGCATC TGAGAGGTGCTTCCCATAGC 078B2A05 AUBES4694 2946 149 8 43.1 ATGCGCACACCCTCATACTC CGGGGGAAATGTTAAAATCA 083A2E06 AUBES4443 2866 193 8 43.2 TGCATGTTTACCATTGCATC TGAGAGGTGCTTCCCATAGC 078B2A05 94 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4401 1907 281 8 43.3 GACACCCTGAGTAAAAGGCTTG AACACGTTTGGTCGTTAGCA 090B2F12 AUBES1610 314 556 8 43.7 ACTCGCTGAAGAAGGCATTT CGCTCACTACATAGGGCATGA 021B1A10 AUBES4591 1868 255 8 43.8 ACACTACCGCTTACCCGAAA TGCAACCTTTATGCTCCTCA 010B2B03 AUBES2448 2632 255 8 43.9 TCCTCACTCAATTTTCCTCCA TTTCTCGGATCCATCTGTCC 040B1B11 AUBES4351 408 720 8 44.0 CTGCATAATACCAAGCAACTTGT TGTGCAGTCGTGAGATGTGA 058A1A02 AUBES1918 2101 419 8 44.0 CCTGTAGGTGGTCACCCTTG TCTTCCATGGTTTCTGTCCA 036A1B06 AUBES5305 2687 169 8 44.1 TGACCCTTCAACTGGAGGAC CCCATGCTGGATTATTTGCT 106B1D03 AUBES3256 376 392 8 44.1 TTCAGCTTCCACACAGCATC TGTCACTATGCTGCCCATGT 054A1D12 AUBES3194 885 1133 8 44.2 TCCTAACATGATTTCCACTGAGG GTAACCAAGGGCTGGATGTG 048B1B11 AUBES3484 388 274 8 44.3 AACAGCATGGGTGATCATAGG GCACAGGTGCCTGTCAGTAA 031B2A04 AUBES1730 1909 203 8 44.3 TCAAACCAGTGTGCTTTCTCC CAGCCAGGAGTCCAAACTGT 026A1G04 AUBES3381 207 502 8 44.4 GACCCTGAGGACTTGGATTG TCCCCAGTAATCCACACTCTG 111B1C04 AUBES1942 302 313 8 44.4 GGCTCCAGAGCTCATACAGG CCTCCCACTTTCCAAAAACA 036B2H09 AUBES5006 1114 288 8 44.4 TCCACAGCATTGAGGTGTGT AGGGTTCAGCAGCAGTTCAT 058A2E02 AUBES3322 1353 392 8 44.4 GCAGGAGAAAGCATCCTGAG ACCCCATGTTTGGGTAATGA 065B1H12 AUBES5090 1515 169 8 44.4 CGTAGCGAAAGTGGTCACATC TGGAAACTGAGGGTGCTTGT 107A1F05 AUBES4627 2248 156 8 44.4 CAGCAAGCAACCATCAAACA TGCATCACACGTTGACACAT 041B1C12 AUBES3257 376 392 8 44.5 CCTCTTGCGTTCTGTGTCCT TCCTCTTTTTCTAATAACTTCCTTTC 083A2D06 AUBES3051 443 582 8 44.5 TTCACTGCTTTCCTCCAACC GCCACATAACAGGACCAGTG 052B2D06 AUBES5330 3027 345 8 44.5 TGAGAGCCCAGGTAGTCCAT AGCTCAGACAAGCCAAGAGC 006A1C06 AUBES2057 1909 203 8 44.5 TCAAACCAGTGTGCTTTCTCC CAGCCAGGAGTCCAAACTGT 039A2D12 AUBES4923 440 489 8 44.5 CAGTGGAGGGCTACAGGAAA TTGATCGATACTCCACCATCC 086B1H11 AUBES3324 443 582 8 44.5 TGCAAATTGCCCCAAGTAGT CCCTGGGATAGGTTCCAGTT 059B2F10 AUBES5268 2525 161 8 44.5 GCACAACATGCACTTCCAGT AGCCGGTGGTGTGTGAAT 027B1B07 AUBES5155 1750 372 8 44.6 CTGCCTTAGAGCTGGATGGA AATGGCCAGTCCGTGTAAAG 054A1G02 AUBES3717 236 795 8 44.6 AGCAGCAGCTCCAGAAAGAG CTCACCGTTCTCTGCATCCT 034B1C11 AUBES2597 236 795 8 44.6 CAGCAGCTCCAGAAAGAGGT TCGGCATGCTGCTAAAAAC 034B1A11 AUBES1786 376 392 8 44.6 ATCACGGAACACACGTGAAA TTCAGTCCCATTACACCGATT 034B2C01 AUBES3485 388 274 8 44.6 GCACCTGGAGTGAACTGAATG TTTCCATTGCATCTGTTCGT 018A2D09 95 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2801 1208 353 8 44.6 TGCCACCCATGCATTTAATA ACAGTGTGCAGTGTACCTTGTG 040B2A04 AUBES4426 2866 193 8 44.6 CCTGGCATTAAGAGGCGTTA TTGAGGTTGTGTTCACAGTGC 012B1B09 AUBES3749 236 795 8 44.6 TGGCAAAAGGTCTTGTTCTTC TCTGCTGAACTCTCCGATGA 103A1E11 AUBES2424 2058 196 8 44.6 ATGGTCCCCTGCAGTATCAC TCCCCTTGGCAGGTAACTAA 045B2H06 AUBES2802 1353 392 8 44.7 TTGGCATCCTTGGTAAATCTG GCAAAGGGAGGTAGCATTGA 022B1B08 AUBES3750 375 401 8 45.0 TGAATTCTTTGGCACCACAT TCCTCACACAGTGGCATGAT 106A2F11 AUBES1763 236 795 8 45.2 CTGCATGTATGTGGCTCCAG TCATGGCATGCATACTAACACA 032A2G02 AUBES1882 singleton 8 45.2 AACAAAGCTTGGCCTTATGC TTGATGATGGTGCTGGAGAA 028A1F11 AUBES2731 33 396 8 45.4 GCCATGCTTTCATCTCTATCC GGTGTCTGGCTTGCTTATGTC 022A1H02 AUBES4442 2866 193 8 45.6 CCTGGCATTAAGAGGCGTTA TTGAGGTTGTGTTCACAGTGC 012B1B09 AUBES3321 1208 353 8 45.6 TGCCACCCATGCATTTAATA CAGTGTGCAGTGTACCTTGTGT 040B2A04 AUBES4507 2914 210 8 45.8 TACAGCTCCCTGCATTCCTC GGCTGTCTGCTTGGTCTTGT 028B2D02 AUBES3855 3491 159 8 46.1 CAGTTTGCCTGTTGCTTTCA TTTTTGGATCCCCACTCAGA 018A1B08 AUBES2263 1931 419 8 46.6 TGCAGGAATCATGAAATGGA CCACATACAAGGCTGCACAC 043A1F07 AUBES4345 159 450 8 48.8 AGTTGGCCATCAAAAACCAG CAGGAAGCGTTTTGACTTGA 085B1F05 AUBES4400 1907 281 8 48.8 TCACCATGCTCAATGAAATGT GCTTGCTCAATGGCTACAAA 028A2C01 AUBES1635 314 556 8 49.6 ACTGTGTTTGCCCAGGTGTC CAGTGGAATGTCCTCACAAGG 023A1B02 AUBES5252 2440 188 8 50.7 TGTCCATTTTCCCCCTCTATT CCAAATTCCCTCTTTGCGTA 047B2A06 AUBES4249 1156 291 8 51.1 CTTACGAAGCAGGCGAGAGT TGCACGAGACTACAGGAGGA 080B1D05 AUBES4075 689 279 8 51.4 AGTGGGGAGTCCGAGAGTTT GCTGCTGATTAGCATATCACTTG 077B2B10 AUBES2886 376 392 8 52.3 CGCTAAATTGCCCCTAGATG CTCTTTGTTCCCATCTCAATCA 054A1D12 AUBES2754 233 424 8 52.7 AATCAATGAAGGGCCTATCG TTGGTGCACTAAGGGGAGAG 029A1C05 AUBES3115 159 450 8 53.0 TAACCCAGCACTGCCTTTG GCAAGATCTTCACATGAGAAACATAA 029A1D06 AUBES5286 2605 315 8 62.1 GTCGAAATCCTTCAGGCGTA CGAATGGTGCTGCAGAGTT 090B2H06 AUBES4778 2851 269 8 65.3 GGTGCCATTCCTTCCATTAC TGGAAGTGCTTTGGGTTAGG 074A2G08 AUBES1287 780 233 8 68.4 GGGCGGATCAGAACCGTCTC TTGGGTCAGTTGACGCCTGG 028A1C10 AUBES2257 9 2006 8 69.0 CGGAAGCACTAAAACCCAAG CCCCAAATCAAGTACAGGAAA 042B2D10 AUBES1283 780 233 8 69.0 ACAAAACGCAGTGGGGTCTG TTGGAGGACTGTTGGGTCAG 006B1B04 AUBES3569 780 233 8 72.5 GATCATTTTCCGGAAGGACA GGGAGCATGTCACCAATCAT 018A2H02 96 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4366 780 233 8 73.2 GATCATTTTCCGGAAGGACA GGGAGCATGTCACCAATCAT 030A1C08 AUBES4000 1080 345 8 78.6 TTGTGACCTTAATCCACACTGC CAGACCTCAGAGACCAGCAA 084B1F07 AUBES5197 2134 272 8 79.1 GAACAGCTATGGGTGGAAGG CATGGTCAGATGGTGCTGTC 104A2H04 AUBES5373 3349 230 8 79.1 TTGGATTTGCTTGCCTACCT GGAGCAAGTGGAGGAGTTGA 011B2F10 AUBES4399 1903 262 8 79.1 TCGAAGACGAGTGATCCTGTT GGAAAGTGAAAATCAAAAGAAAAT 011A2H01 AUBES5231 2359 337 8 79.5 GACATGTATCTGCCGATTTCAA TAATGCCTGCATGGTTTTCA 084B2G10 AUBES4493 1660 203 8 79.5 TCTGATTGTCATCTCATTGCTTG TCGAATTTTGGAGGATTTGC 075B1G06 AUBES2204 1345 198 8 79.5 TGTGCTCAGTGGGTTTCACT GCATTCATTGATTCCATGTCC 030A1D11 AUBES2316 2068 254 8 123.2 CAAATGTCCACAGGGAGCTA ACACAGGCATGGTCAGTTCA 026B1B11 AUBES5156 1752 142 8 127.8 CACAGGAATCACAACGTGGA TCTCTGGACATGGTGTGGAA 014A2H10 AUBES5199 2139 220 9 0.0 CTGCTCACCTGATTGCACAC TCGTAGCGTTCAGGGAAAAA 072A2G11 AUBES5227 2331 193 9 1.5 GGGGCTGTTTGGTTAGTTTG CTTTCCCGTGTCATTCCACT 107A2G11 AUBES2104 920 306 9 2.6 TGGAATGATACCAGCAAACA GGGGGAATCATGACAGCTAA 041B1E01 AUBES3833 1881 316 9 5.8 AAAAACCCCCACAACAACAG GTCCAGCTCAACTGCAGGAT 076A2H04 AUBES1600 singleton 9 6.2 CTCCCTTCACAAGCTGTTCA CCGGGGCTTCTATAGCACAT 024A1A03 AUBES3819 1684 391 9 6.6 CCCACATTATGCCTTCACG TAAACTCTGCAGCCTTCCAA 105A2E09 AUBES1761 981 693 9 6.9 GAAACACTGGGGATGAGGTG GCTGCATGTTCCTGAGGAGT 028B1D05 AUBES1607 1299 298 9 6.9 CCACCATGTGCCCAGTCTAT CCAGTCCGACATAGTGAGGA 032B1F02 AUBES1871 singleton 9 7.0 TTGGATAGCTCACGGTGTATG CATGGACGCATTGTAGTGTTG 025A2E02 AUBES4658 176 330 9 7.4 GCCTAGTGGCACTCACATTG CTCCTCCCAAATGCTGAAAC 058B1F03 AUBES5357 3276 244 9 7.5 CACATTCATGCAGTGTTTGG CCTTGCTTCCCTGTTCAGAC 079B1C08 AUBES3055 490 753 9 7.6 ACTGAACACCAGTCCGTTCC CACACTTCCATCTGAAAGACACA 072B2G01 AUBES1843 176 330 9 7.9 GCGAGTTGGAGACCACATC ACTTCGTTTTCCCCCAGAAG 036A1C08 AUBES1435 singleton 9 8.0 GTGACATGGCTCTGCTAGGC GCTGATGCCATGCTAGTGTT 020A2C05 AUBES4986 991 227 9 8.0 AATCGGTCAGATGTGGAGGA TGCCTGTCTGTATCTGTCTGTG 020A2F07 AUBES3025 502 494 9 8.1 TGAGTGGGAAAAGTATGTGAACC GCATCTGTCACATTGCGTGA 052A1G09 AUBES1348 814 516 9 8.2 CCCATTCACCTCGACATGG GCGCAAGTTTAAGGATGTGTGC 006A1C09 AUBES3048 985 573 9 8.4 AACAACAGAGTGTCTTCCCTCA AGATTCAGGATTTTCCGTTCA 075A2G03 AUBES3533 981 693 9 8.5 ATCATCTGCCTGAGGGTGAA GTGCTGCTGAAGTGGACTGA 100B2G05 97 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4642 465 249 9 8.5 AATAAGGCAGCCAACAGTCG AAAACAGGAAGTAGAGTGATTTTCA 028B1D06 AUBES4166 1532 450 9 8.5 CGCACATTTTGAAATCTCTCC TTTAATCGTGTGTATTTGAGTATCG 058B1A07 AUBES3253 300 428 9 8.5 GACCGTGGCAGGTATCAAAC GCAGCTGTCTAGCGATACCC 078B1A08 AUBES4639 390 252 9 8.5 GCAGAGCGATATCAAGCAGA ACGAAAGACCCACCTCTTCA 072A1C12 AUBES3259 419 524 9 8.5 CGAATTCTGGATTGTCATGC CATCGTGGTCGACATACTGG 068B2D08 AUBES2561 490 753 9 8.5 TCGAGGTGTCCAACAGTGAG ACAAGAAGTGATGCGCTCTG 025A1E06 AUBES2916 879 345 9 8.5 CACATCATGCAAAAGCCTTC AGAAAAACGAGAACAGACAGTGG 005B1E04 AUBES2051 985 573 9 8.5 AGTCAGGGCTAAAGCTGCAA ATATCAGCTCTTTCACCAGCTT 039A1H02 AUBES2103 1401 624 9 8.5 TCTGAGCTCTGACCCCAACT TTTTACACGTGACCAACCTCA 041B1D07 AUBES3781 1684 391 9 8.5 CCCACATTATGCCTTCACG TAAACTCTGCAGCCTTCCAA 105A2E09 AUBES5245 2416 222 9 8.5 TGAGCAAAGAAGGCTGTGAA GGTAGCTGGCATGGCATT 044B2H09 AUBES5343 3221 144 9 8.5 GTGCATCTCAGGACAACGAA CGTTCAAAAGGATTGTGCAG 074B1G04 AUBES2809 176 330 9 8.6 ATTTACCAACCGCCAGATTG TTACTTCGTTTTCCCCCAGA 036A1C08 AUBES1343 1884 230 9 8.6 CAGCATAGGCGTCTGGCAGG AACATGGCCCTGTGGTCAGG 006B2C09 AUBES3166 42 1038 9 8.6 GTCTCGAGCAGGTGATAGCC AGACGTCCTGTCTCGGAAAA 055A2C08 AUBES3105 154 1130 9 8.6 GAGCTCTGGAAAGGCCCTA AAAACGCTTGCAGACCAATC 064B1H04 AUBES3970 1686 162 9 8.9 CCTGCAATGCTACAATACCG TCCAAATGAATGGATGGATG 005B2E10 AUBES3818 1684 391 9 9.0 TGTTTAAAGTTGAAGTGAATGGAG CCCAAAGACTACGTGCAAGA 038B2B01 AUBES3118 490 753 9 9.0 CGGGGCAATTAGTTTTCACA TTTGGATAAACCCGTGTTCTG 048B1B12 AUBES4666 680 348 9 9.0 TGCAATCGAGCCAGTTACTC GGTGTGAGGCAAGTGTGCTA 088B1B06 AUBES4280 1684 391 9 9.0 TGTTTAAAGTTGAAGTGAATGGAG TCCCAAAGACTACGTGCAAGA 038B2B01 AUBES2668 751 391 9 9.0 CCTGCAGTGGAAAGGTGTCT TGTGGCTGATGTCTCCTTTT 064B2F12 AUBES4973 881 271 9 9.0 GCTTAACTGTCAGTGGCATTC CCTTTGGTTCTGGTAAGGTTG 079B1B03 AUBES4689 2889 167 9 9.1 TTTTGACAAAGCATGGCAAG TGACAGAGAATCAATGGACTCATAC 010B2D03 AUBES2938 124 262 9 9.4 TGCCCTATAGCCAAACACAA TTCCTGTGGACATTCAGCAA 009A2H08 AUBES4274 1565 311 9 9.5 CAGTGGTACTCAAAGTGGGATG GCTGCCATAGACACCCTAGC 001B1D09 AUBES3720 1401 624 9 9.5 AAGCTCTCCTGGGCGTTTAC GTGCTGCTGGTTTGTTTGTG 046A2D01 AUBES2709 1401 624 9 9.9 GCCCGACTATGGAAATAACG TTAACTGCCAAGCCACCACT 006A1D02 AUBES5391 3421 188 9 10.1 AGGCATTTCCTTCACTGTGTC CGACAGTTAGCCAGCTCAAA 090B1C02 98 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3162 154 1130 9 10.4 ACACTGCGTGCTGAACAGAG TTGCCCACGTGACATTTAGT 103B2A01 AUBES5257 2483 188 9 10.8 TCTTCTGCTATGGTGGAACTGA TTGTCAGAGGAACCAGCTCA 096A1G02 AUBES5055 1337 340 9 10.9 GCGTCTCGTGGAGTTCACTT GCGAGACTGCCGATACAAAC 050B2G09 AUBES3499 618 264 9 11.2 ACACACAGCCCACCTCTTTC GTTATGCAAATTGGCCCTGA 045B1C11 AUBES3817 1584 269 9 11.9 GAGCAAAGGACAGACACACA GCTTGAAAGTGTCAATGCAAA 048B2E07 AUBES2893 539 614 9 13.9 CTGACCGGAGAACCCATATC TATGAACATGCGTGGCTGAT 058A1G07 AUBES5347 3242 218 9 15.2 TAACAAATGAATGGGCGAAC GATCCCTGGGGAGAAACACT 083A1E10 AUBES4060 409 421 9 16.2 GCTTCTCAAACACTCCTCAGC AGGGAATCGTTACGTGCTGT 023B2G08 AUBES1410 409 421 9 16.5 AGACCAGATGCATAGGTGAGC GGGAATCGTTACGTGCTGTT 023B2G08 AUBES4080 765 311 9 17.1 CACCATCAAACACTGGCATC CCTCATGCCCTGAAATATGC 003B1C01 AUBES4840 1554 353 9 19.3 GGCGAGGATTAATCGTGATG ATCTTTTGAGGCCAAACAGC 027B1A02 AUBES4659 176 330 9 21.6 CGACAGAGCTGGAGGGTAAG TGTGTCCCATAGCAGGTCAG 063B1E07 AUBES5078 1433 296 9 25.8 ACCGTGCAACAATACAATGC TGTATGATCTGCGTGCTGTG 001B1G10 AUBES4571 1817 252 9 26.9 TGCCATAGAGTGAGCCGAGT GGGGAACCTTTGTGAACCTT 070A1A06 AUBES3653 72 1338 9 27.9 GCACCTCCTGGTGACGATA CCGAAGGCCTTACAGAACTC 062A2A11 AUBES3716 72 1338 9 30.4 GAAATCCAGTCAGGGTCGTC TGTTGAATGAGGCTGTCGTG 030B1G12 AUBES1368 1484 350 9 30.6 GAAATCCAGTCAGGGTCGTC TGTTGAATGAGGCTGTCGTG 030B2G12 AUBES3713 72 1338 9 30.6 CCCAGAACCCCCACTAGAAC GGAATGATCAAGTGCACCAC 028B2A05 AUBES4815 3100 223 9 31.6 ACCAAAAGGCCAGGCTAATC CCACGATGTCGTCCTCTAGC 031B1E06 AUBES3229 72 1338 9 31.6 ACCGCTCCTCCACAGACTAA CCCAGTTCCTGTAGGTTTGC 011B1A04 AUBES4501 2513 304 9 32.0 CACACCATTCCGTACACGTC AGCAGAGGAAGAGTCGAAGG 072A2F05 AUBES2379 72 1338 9 33.4 TGAGTAGCTCCCAACGATGA TGCGTACAAGGCCAATTATG 108B1C02 AUBES3373 72 1338 9 34.5 TCGTTCCTCTCCTCCATCTG CAGATCAGATTCCCCGTCTC 096A2F05 AUBES3199 72 1338 9 36.5 CGCTGTATCTGAGCGATGAC AGTTGGGGGACACCCTAGAC 049B1F09 AUBES1436 205 465 9 51.3 GAACCACTGTCTTGCAATGTG CCCCTAGGGAACCTGAACAT 020B1C12 AUBES3230 72 1338 9 57.3 GTGTCTGAAGGGCCAAAGAG CGCTAATCAGTCTAAATGCTTTC 055B1F02 AUBES1885 singleton 10 9.4 ATCCACCCCCTTGTCTGACT CATCAGGCTTTGAGCAACTG 028B1H10 AUBES4226 660 260 10 11.0 CTGCTGGAAGATCCAACCAC GCAGACCACCATGACATCAC 023A1B10 AUBES4677 2807 198 10 11.7 TTTGGTGAACGGTGTGACTC TTCCAGTGTGTGGTTTTCCA 024A1D02 99 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4506 2804 430 10 20.6 CTGTTCCAGCACATTCGAGA CGGATATGAATACGAGGAGGA 007B2F04 AUBES2260 1261 382 10 31.2 ATTCATCTCGCAGGAAGCAG TGCTCTGCACTTTTGTTCCA 043A1B01 AUBES3739 904 342 10 41.8 CAGCTCACTCGGTTCTGTCA CAGCAACGCCTATCACACAG 082B1D07 AUBES3317 904 342 10 43.5 AACCCCTGAGCATTAGTCCA TCTGGGTATCTGGACAGCAC 059A2C11 AUBES5050 1329 196 10 44.1 GCCAGCAAAAATGGATCTACA CCAGAGATCGCACTTCCATTA 071B1G07 AUBES3769 661 638 10 47.8 CCTTTATGGATCCGGTAAAACA TCAGTCTCCCCACTCACCTC 077B2C04 AUBES3302 559 326 10 48.9 TACGGGGTCAACCTCTTCAT ACGTTTGAGAACCCCTGGTC 088A1D06 AUBES2437 559 326 10 51.3 AGACCAGGGGTTCTCAAACG GCCTCTAATCCGAGGAGCTT 040A2B12 AUBES5056 1346 266 10 55.9 CAAGAACCAGTTGGGTGTCA CACGTTTACACTGTGGCTGAA 045A2B08 AUBES5311 2735 296 10 59.0 ATTGAAAGGCAGTGCAGGAG CGTCAAATAGTACTGAAGCAAAGC 060B2C05 AUBES2580 436 854 10 59.9 TGACATCAAAGATGCCCTCA CAAGGCGTCAAAGAGGATTC 072A1A03 AUBES3070 436 854 10 60.5 GCACATCCCAGAACAACCT ACTGTGCCCTGTAGTTTTGGA 111A1F02 AUBES1949 327 463 10 61.7 AGACCTCCATAGGCCACGTC CATGCTGCTGATACGACTCC 020B1E12 AUBES2523 48 269 10 63.5 GAGAGCACACAGACACATGC AGACTCCTGCCTAGCACCTG 048B2B06 AUBES1867 37 572 10 64.7 TGTTCACACACATTGCTGACA TCTGGCAACTTTTACAGTGCAT 023B1E10 AUBES2863 89 387 10 65.2 CCAGGATTGCACATTGTTGA GCTCGAGTGCACACTGTCAT 030B2G11 AUBES3225 37 572 10 65.3 TGAACCTTGCTAACAAAACGAA CTTAAGCTGTATTGCAAAAATAGGA 020B2A05 AUBES4924 457 343 10 65.9 GGACAATTAAACGCAAAATGC GTTCCGATACGTGCAGCAG 012A1H04 AUBES4612 2063 222 10 66.2 AGACGGTGGACGTAATCGAG CATGCACGGCCACATACTT 112B1D12 AUBES2906 820 605 10 66.5 CTGAGGGAATCTCTGCAAGC GCAGTGAAGCCGATGATACA 077A2G06 AUBES4956 729 316 10 66.7 GATGGACTGGCACCCTGT GGCCACTCCACATAAACCAA 112A1E05 AUBES3500 625 326 10 66.9 GCACGCACACATACAAACCT TGGGATTCTGGGAGCATTTA 096A2B11 AUBES3501 625 326 10 67.3 CATTCATCAATGCAGCCAGT TGTCATTGTTGCTGCCATTT 104B2E02 AUBES4850 1748 592 10 67.7 CACAAAAGGGCAGACAACAG TTAGTTCCTGGTGGCAAACT 035B2F04 AUBES3600 1315 389 10 69.2 GGTCTCGCTCCCAAATGTAA TGGAAGATTTTAAGGCAGTGG 066A2D06 AUBES3939 89 387 10 69.2 AAAAAGAAGGGTGCTAAATGGA ATGCGCATAGGACATTGACA 069B2D05 AUBES4665 611 372 10 69.5 ACATGGACAGCTGCTGAGTCT GAACAAAACAAAAACGCCATT 100A2A11 AUBES5304 2680 282 10 70.0 CGAGGTTGTAGTCGCTCGTC GAGTGATGAGCTCCGCCTAT 052B2E01 AUBES3601 1315 389 10 71.5 GGTTGGATTCTCTGCTGGAC TTGCAAGGACCTGATTTATCG 043A1A07 100 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4478 1495 250 10 72.2 TTGCAGGGAGGAAAAGTGAC TCTCTCCATCTCTCCCTGTCTC 029B2F06 AUBES3980 1798 433 10 73.5 GAGGTTCCACACTGGCATTA ATGCCTAGCCTTTCACTCCA 079B2C09 AUBES5016 1174 260 10 76.1 TTTGTCAGTGGGCAAACGTA TGTTTCACGGACATTTCCAA 063B2D09 AUBES5309 2721 107 10 76.1 GGTTTGCAGTATTCAGGTGTGA TTGCATTTGACTGCTGATGTC 060B1C06 AUBES5224 2318 249 10 76.6 GGTGAAAGTCAAACACGTACAAA ACTGGAGGGTTGATCCACAA 109B1B04 AUBES4407 2112 232 10 76.8 TGTAGCTTGTCCTGTAATATGCAC GCTGCAATTATCCAAGATGG 045A2D03 AUBES5319 2766 288 10 76.8 CCTACCTCAAGGCACAAGGA TTGCAGCAAAGGACAGACAC 080B2D11 AUBES3632 458 318 10 76.9 CGTGGTGGTTGAGCAGATTT ATCGAGACGATCTTGCCACT 081A2B09 AUBES3942 111 462 10 77.0 TGTTGGTGGTGCTAGAAGGA TTCTTGGGGGATTGTTCTTG 075A2E01 AUBES3545 381 267 10 77.0 CTGCGTCACATTTTCAGCAC CTGACACCTGCCATCAAAAA 044A2D08 AUBES3631 458 318 10 77.3 CCATTGGGATAACGTGGTCT GAGATAGCCGGTGGCACTT 032B2A03 AUBES4828 3173 169 10 77.5 AATAAGGCGTCTTTTTGTTTCG CCACGTGTGACTGGCATCTA 107B2B04 AUBES3412 381 267 10 77.6 TTATTCCAAAAGGCCTGGTC CAGTTGATTGCTGATGCCTTC 032A1E06 AUBES3943 111 462 10 79.5 GTTAATGCCGACGCTGTTG TTTTAAAGTTTGAAGAATTTACTGCTG 029A2G06 AUBES2444 3325 353 10 84.8 CCATGTTTGAACCACAAGTTTC ACCGGACGAGTGGACTGTAG 040A2H09 AUBES1904 384 269 11 23.7 CAAGCAGGAATTGTCCATGA TCCTTCATTCCTCGTGTGC 036A2D12 AUBES1864 77 499 11 25.1 CGGTCCACAAGTGGTAAAGAA TTCCCCGTATGAGTGTAGGC 022B1A02 AUBES4756 2642 210 11 26.7 GGGCTTCACATCTGACCAAT GAAAGAATGGAAGGGTGTGG 061B1H10 AUBES3424 471 458 11 29.3 TACAAAATGGTGCCCACAGA CGAGCTACAAATTGGGGTGA 112B1C10 AUBES3649 793 279 11 29.3 CCAGCAAATGTCAGGGGTTA CTGTGAATGGGCTGTTGCTA 007A2E07 AUBES3425 471 458 11 29.6 CAGCTTTCCCGAAACGTAAA GTGCATTGAGAAAGGCACAG 056A1A06 AUBES3204 266 482 11 38.0 GTTTCCTCCCACAGTCCAAA GTGAAATCCTGGAGGGACTG 083B1C10 AUBES2944 313 330 11 39.4 TGTAAGTGAGCACCGTGAGC CGTGTTCTGGGTGAGTCTGA 041B1F03 AUBES1859 20 693 11 40.3 TCACACCATGTGCTTCAACA TCTGATGCCAGAACTTGTGC 019B1B01 AUBES2859 41 502 11 41.0 TGGCAGTGATCCATTCACAT AAATCCCGTGGTTTTGAGTG 056B2G06 AUBES4763 2714 222 11 41.3 CCTCAACAATTCGGTGTGC TCCCCCTTCTGCAAGATGT 054A1B01 AUBES3712 682 474 11 41.3 CCAAGGGACATTAACCCTGA CCCAGATTTTCAGCCTTCCT 023B1B12 AUBES2816 367 272 11 41.6 CCCTGGGAGACATGAAAGAC ACAATGGCCTCTGGTGAGAC 057A2F10 AUBES4475 1330 205 11 42.3 AATACGTGCGACTTGTCCAG CAAAGGGTTTCCTTGTCGAG 098B1C10 101 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2979 41 502 11 42.6 TTGTCAGGTGTCCACATGCT GGCTTTCCAAAATGTTGATGA 106A1A05 AUBES2601 20 693 11 42.7 TTCCGAAGGAGTCTCCAGTG GGTTGCAACAGCTACGAACA 053A1A05 AUBES4231 826 370 11 42.9 TTCTTTCTCTCGCAATCTTCCT ATTGTCCTCGCTTTGGAAAA 058B1B06 AUBES2371 626 712 11 43.1 TACACGACACACGGCTATGG CACTTTCTCAATCTCTCTCTTTCG 044A1B02 AUBES2712 594 477 11 43.4 CCCACGGACACACAAATAGA GCAATGACTGTTTTGCACCA 008A1F01 AUBES4781 2876 161 11 43.8 TGGCTGTTTGATGGATTCTG TGTGTGTGCATGCTCTGTGT 054B1B06 AUBES2512 1716 580 11 43.9 CTTTGGGGGAACGTCTCACT TACAGCTCCGGTAGGCAATC 048A2E05 AUBES3527 646 524 11 44.1 CACGCCCTGTCGTATATCCT AACTGATGAGCACGGGTAGG 042A2D10 AUBES3577 941 281 11 44.2 TTGTCTCAGCCAAGAACAGG GGGACCAGGGTAAAGCAGTT 077B2D01 AUBES4372 1178 316 11 44.2 AGCTCCATGGATGGAATTTG AACGTCAGTTGCTGGGATTT 038B1G07 AUBES5141 1681 227 11 44.2 CAGGCTCCCTGCAATAAGTG GCATCCATCCATCCAATCAT 083B1B09 AUBES4491 1655 211 11 44.2 AACAATTTCCATGGCGTGTA AAGTGGCACAGGGAAAAGAG 100A2D10 AUBES3867 801 558 11 44.3 CTGTTACAGCTGCGAGATGC TGCTGCCTACAAGTGACAAAA 032A2E08 AUBES3872 801 558 11 44.3 TCAGGCCAAGGTTACCAGTC TCCAAACAGAGGAGGCAGTT 090B2G11 AUBES3448 875 347 11 44.5 GCTGCTGAGAGTGGTGGTAA AAAAATGTGAGGGGTGTACTCA 008A1D08 AUBES2717 503 597 11 44.5 TGTGGTGAGAGGCATATGGA CGCTCTCAAAGCAGAAGACA 011A1C02 AUBES4293 1743 235 11 44.5 TGGTGAACAATGAGTGTCAGG ATAGCCGGTTGTGCTTCTTG 002B2A09 AUBES2520 303 279 11 44.6 TTCTCAGCAAGATCCAAACAGA TGGACATTGTCAAAAAGCAG 048B1E02 AUBES5041 1277 235 11 44.6 TGGGCACTTTAGGAAGATAAGG CCAGTGGCCACAGAGGTAAT 024B2H11 AUBES5255 2471 169 11 44.6 GATCCAGTGACCCAGTGAGAG CCTGTTGCGTATAGCAGTTCA 082B1F09 AUBES4315 1958 151 11 44.7 CCATTCTCATGCACAAAAGC AACCGAATATGTGACCCACAA 030B2E08 AUBES3665 646 524 11 44.7 CACGCCCTGTCGTATATCCT AACTGATGAGCACGGGTAGG 042A2D10 AUBES1377 1525 660 11 44.7 CAGTTACAAGGGGTTTCCCAG CACATACACACCTCCATGAGC 025B2F07 AUBES2123 646 524 11 44.8 CAGAGATGACACTATTGACTTAAAGC GTCGACTGGAAGAACCCTGA 018B2A11 AUBES2076 2771 233 11 44.8 TTCCCGTGTGTGTTATTCCA TCCTATCTCGGCAAGAGTCG 039B2D11 AUBES3871 801 558 11 44.8 ACATGCCGACAATTATTCCAG GCTTATCACACGCGCTCTTT 090B1A10 AUBES3914 1075 230 11 44.8 TGATCACTGCAAAAACTGTTCC TGAAGGCTGAGTTGGTTTCC 005A1G12 AUBES4373 1178 316 11 44.9 AGCTCCATGGATGGAATTTG AACGTCAGTTGCTGGGATTT 077B1D02 AUBES3474 225 267 11 45.0 GCGCTGAACAGAACTGGATT CCCAAGAACCACTGAGGAAA 008A2G10 102 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3090 161 626 11 45.0 GAAATTGAAACGGCTCTTGC GATGCTTTGCCATGCTTTAAC 020A1H02 AUBES3050 561 533 11 45.0 GCTGCACATAATGCAACCTG TGCTGAACAATCCTTTGCTG 077A2E08 AUBES3714 709 387 11 45.0 GGAACCGGCTGAACATAAAC TGGCACTGATTTGGCTAATG 029A2D03 AUBES2670 831 413 11 45.0 TGACAGAACACAAGGCCAAG CATAAAATGTCTTGGTGTCATCCGC 045B2C10 AUBES3775 1168 281 11 45.0 CCACCACCATGTCCCTTTAG TGTGAGATTGGAAACGGTTG 078A1C07 AUBES4385 1698 260 11 45.0 GTTTACCACATTCATGCCAGAG TGATGTGAGTCTTGCCAATCA 026A1F10 AUBES3837 1975 194 11 45.0 GAGTGCACCCGGCTTTAGTA TTGGCTCTTAATGCTCTGTCC 090B1G09 AUBES5361 3288 235 11 45.0 CGAGGGGGAGAGACACATAC CCGTTCTGCCTGTTTTGAGT 095B2F10 AUBES4294 1743 235 11 45.0 TCACATGTAGGGCATGTGGT TCTGGTGAAAATGTCCTCATTG 106B2G02 AUBES5019 1187 267 11 45.2 TGTCCAAAACTTCACCAGCA TTCTCTGTGCAACCCTCAGA 012B1D05 AUBES1137 515 414 11 45.4 ACATACCTGACAGGTGAGTTCC ACTGAAAGCATCTGTTGCACG 007A2E10 AUBES2266 singleton 11 45.6 CACATGAGTGAAACAGCGAGA TGTCCCACCTTGTTGTATGG 043A2D07 AUBES3473 225 267 11 45.7 GGCCCTGTTTCAAAGATGAT GGGGGTGAGCAATACGACTA 073A1F08 AUBES5258 2486 191 11 45.9 CAGACATAAATGCTTCGAGTGC TTGCTGGATTTCCTCTTCACT 003A2C08 AUBES1538 425 228 11 46.1 TTGCATCGGTGCATCTCTAA TGAGGGGTGTACTCACTTTTG 030B1G03 AUBES2610 161 626 11 46.6 AACCAATAGACCAAACAAATCAG GGCACCAATGCCAGTCTATC 006A1G10 AUBES2703 17 543 11 46.7 GCAACTGAAGTTTGCCTGAA GCAACAAAGAGAGTAAGGGTTAAG 026B2C08 AUBES5372 3336 112 11 47.0 CTTGAAGTTGGTGGAAAACAA AAGTTCATGGCACTTGCTACA 020B2E06 AUBES1183 2034 213 11 48.2 TTATTTGGGGAACGGCAGTG TGACGTATGGTTGGCAACAC 011B1C04 AUBES4829 3181 318 11 48.4 CAATCGTTGCATTTATGTAGCA CAATGGAGAAAGGGTTGGTG 073B2F11 AUBES3866 677 360 11 48.8 CCCCACAGTTGGTACCTTTG TGGCTCTCAACGAGAACTTG 082A2E11 AUBES3648 793 279 11 49.4 ATCAGATTGGCGAGGTGAAC CAATTCGACCCCTGTAAAGC 051A2H09 AUBES3315 724 431 11 49.9 GCCCTTGGTCAGTTTATCCA ACCAAAAGGTTTCTTTGAACTAACA 078A2F05 AUBES1765 941 281 11 51.2 GCCCTTGTTTGTTCATGTCC GGGACCAGGGTAAAGCAGTT 032B2H02 AUBES2481 248 712 11 55.2 GCGATTCCATGTTGTCTTGG CCTGCCAAACATGACACAAA 046A2C04 AUBES3480 282 408 11 61.1 TCCACTCCAGCATGTTTCAT GCCGTGTATTGGTGGAATGT 096A2G11 AUBES4643 472 222 11 70.6 CTGGCACGTGTCCAATCTT TGGAAGTGTGAGCATGATGG 023A1E02 AUBES1612 singleton 11 71.3 TACCAGCCTTCCAAGCATTC GTCTCTTTTCGGTCCAGACG 024A2B01 AUBES2844 360 416 11 71.9 CCCTATTCATGTGCGAGTCA GCCATTTAAGAGCAGCGAAC 003A2D05 103 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3278 47 315 11 72.7 GCGATACCCATGATCAGCTC AAAAGCAAACTAACAGGCAAAGA 090A1C02 AUBES4744 2509 167 11 73.6 AGCTGCAAGCCAAAGACTGT GGCAGTGCGGAGTCTGATA 030B1H07 AUBES4192 360 416 11 78.7 TTCCCATGAAGGTGACTCGT CGCAGCTGGAGTAGTTCCTT 013B1A04 AUBES2217 360 416 11 81.2 AAAGAGAGAGACCGGGAATG GTCTCACTCTCTCTCCCCATC 032B1D10 AUBES2525 1630 157 11 83.9 GCCGTGCCTCTTCTTACTTT ACACACCCGCAATCTTTTCT 048B2C01 AUBES4575 1865 191 12 0.0 CGCTAAGTTCCTGCCTTTTG CCTTACACCAACGGTCACAC 106A1H11 AUBES1407 1197 206 12 18.1 CAACCCCTCATTCACGAGAT TCAGGTTTGCACATCACTCTG 022A2B12 AUBES4384 1646 235 12 19.4 GCTGCAATTCGGAGCATAAT GGGTACTGGAGTCCCACAGA 108B1F10 AUBES4827 3171 223 12 19.9 GCTGAACTGATCGTTGTTTTTG TCAAAATGCCATGTCTGACC 109B2C10 AUBES2374 3376 240 12 20.6 TTGAAAATGTATTCCAAATAAGGAC TTGACCATGAATCTCATTTTGC 044A1D03 AUBES4383 1646 235 12 21.1 GCTGCAATTCGGAGCATAAT GGGTACTGGAGTCCCACAGA 011A2D06 AUBES3612 1592 460 12 21.3 GAGGATGCCTTGTCCTAACAT TGGCATTTTGCTGCTGAATA 013A1A01 AUBES1764 2495 264 12 22.5 CAATGTCGAGGCCAATCTG AGCACGGAATGACTGCTTTT 032B1H08 AUBES5287 2606 181 12 23.3 GCGACCTGCCGCTTTATTAT GAAAGCGTGCTAATGCTGCT 030B2D09 AUBES4586 1965 194 12 24.2 GGCTTGTGCAGTGTTGATTG TGCAGTTCCTGACTGCTGAA 108B2H08 AUBES4713 3196 301 12 24.9 CCAGAGACCCCTGCTAATGA AGGGAGCTTGAGTGTCATCG 049A1D08 AUBES4714 3196 301 12 25.1 GAAGGCAGATCCCATCTCTG TAGACAAGAATGCCCGAGGA 079A1C11 AUBES2211 1435 203 12 26.7 GGTACCACCTTTTCGCCATA GATATTTCAGCAGGGCAGGA 031B1D12 AUBES5054 1336 353 12 31.5 TCGCGGTCCTCTCTTATTCT ATCCCTCCAGACTCGCACTA 034B1D07 AUBES2406 singleton 12 32.1 TCTCGTGTTATCGCAGCTACA CCACACCAAATTGGCAATAA 045A2C09 AUBES1396 singleton 12 32.1 GCTGCTACTCTCGCATGAC TGTAACCCTAGCCAGCTACAG 018A1D08 AUBES2420 518 531 12 37.0 GGCAGGAGGATAGCAGAACTC GGTCACATTGTTTGAGCCAGA 045B2F06 AUBES3342 194 595 12 37.1 GCCATTTACCGAGAGTGGAG ATTCCCGTACAGCAGAGCAC 068B2C04 AUBES3049 271 555 12 37.1 TTCCAGACTACTTTATCACCAGAA ACACAGGCCTCGGGAGTAG 076B2D04 AUBES2571 518 531 12 37.1 GACTGGTGATGGAAAAGCTG GTGGAGATTCACCCCTTCTT 010A2H08 AUBES4069 546 367 12 37.1 AGTGAATCCTCAGGCCCATA TTGGGGTTGTAATTGTCAGC 111A2D04 AUBES3124 518 531 12 37.3 TTGAATGCCGTGAAAATGTC GCAACTGGCCCATTATCCT 034B1B11 AUBES1888 271 555 12 37.9 CCTGTTCCCAGATCAGAAGC GTGACGGAGCCTGTCTCTCT 029B1H08 AUBES2075 194 595 12 38.0 TGACCCTGTTTTCCAGCTCT AGGGTCACATGCCACAAAG 039B2C06 104 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5186 2025 184 12 38.1 TGCCGAGTCCAGAGAAAGTT GCGACCAGTGCCAATATGT 004B2D02 AUBES4949 669 309 12 38.1 TCGCAATGGCGCATATATTA TCAGCGTCTGGTGGAAGTAA 004B2A09 AUBES5011 1138 244 12 38.4 ACTCATTTCCGTCTGGCTTG TGATGCCGCAGTCACAAC 048B1C06 AUBES4232 828 211 12 38.7 GCATGTTCCTTTCAGCCTTC CTTACAGCCCTCCAGCAGAG 107A1G04 AUBES4913 334 228 12 40.0 TATGTGACTGCCGGTGGTAG CTGTTCCCCTTTGCAGAAAC 083B2G11 AUBES4233 828 211 12 40.5 TTCGTGACTTGAAAAACAGCA AATTTCGTGCGAAGCCTTTA 107A1G04 AUBES2277 27 430 12 41.9 GGCTGCTGGGAACTGTAGTC CAGATGCTCGATGAGCTGAA 043B1G03 AUBES3201 166 890 12 42.1 CCTCCAGGAGCTTGAGATTG GGCCATAGCGATAAGAGCAC 053B2D03 AUBES2664 533 477 12 43.4 GAATGGTGACCAGCTTCACA GTCCTCATCTCTGGCCTTTG 022A1B10 AUBES1726 609 502 12 44.3 GGTACACCAGTCCATTGCAG CAGTAGGTGGAATGGCCAAA 023A1F08 AUBES2050 169 715 12 45.5 CAACTGCCATTCTGGGAAAT AAGTGTTGTGGCTTGAGTGAGA 039A1G05 AUBES4919 422 203 12 45.9 GGAAGCTTTTGGTTGCAGTT CAGCATTCGGTTATCGGTACT 108B1G04 AUBES1273 3217 157 12 46.1 TCGTAGATGGAGGCTCCGAG TGTGAGAACAGTGACCGTGC 028B1C09 AUBES3542 217 289 12 47.1 CTTCTGAGCCCATGTCACCT GCCTGTCTCTCTTCAAAAGCA 071A2E10 AUBES4928 524 337 12 47.1 TGCCAAAGGTATGCTCCCTA CACCAGTAAATGCACCCAAT 025B2G06 AUBES3708 601 386 12 47.1 CCCTTAATTCTGGCTGGTTG CAATTCTCCGAACATGAGCA 019A1B02 AUBES3233 609 502 12 47.1 CTGCTTGAAAAGCAAGATGAAA GACACCCATGATTGCGTCTA 091A1C08 AUBES4888 897 274 12 47.1 GAGAGGGACATCCGGTCTCT GCGCATAGATTTCCAGTTGC 054A1H05 AUBES4887 897 274 12 47.1 TTGAGTGCGAGTGTGAATGAG CCGTGGGGATTTACAGATCA 005A1D11 AUBES5116 1539 198 12 47.1 TGCACTTTGCACATATCAGG CACCAACCCATCATGGAATA 026B1A09 AUBES3967 1673 497 12 47.1 CACCAGCACAAACAGAGCTT TTTTGGCCTGGTTAAAAGACA 087A2C07 AUBES3622 228 279 12 47.1 GGTGCTTTTTGTGCATCCAT TCAGGCACCATCAGACAAAG 110A2H03 AUBES4225 629 277 12 47.1 CTGGAAGATAATGGCCGAAG AAACACAGCATCTGCATTTGA 005B1G10 AUBES2773 601 386 12 47.1 CCATGTCCCTACAATTGCTGT ATCCATCCATCCATCCACTC 013B1G07 AUBES2115 singleton 12 47.2 TGACCCTCAGGTCAGTCACA CACCGAGGATATGTTCGAGA 041B2G01 AUBES2579 609 502 12 47.2 CAGGTCCTGGGTGGAATGTA CAAACAAGCCCAGAACCAGT 012A2E09 AUBES1725 217 289 12 48.1 CCATGTATCAGGTTTGCACATT TGTCATGTCAGTTGGAAGCA 022A2H05 AUBES1345 singleton 12 48.4 GCTCATTCACAGGGACTTCAC TGGATCAATCTCAATCAGGTCAGG 002B1E01 AUBES4138 1073 227 12 48.8 TTCTCACAGCCTTCTTTGCTC CAGCTCCAGTTTCCTGGTTC 023A2D06 105 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4101 1073 227 12 48.8 TTCTCACAGCCTTCTTTGCTC CAGCTCCAGTTTCCTGGTTC 023A2D06 AUBES3054 15 539 12 49.0 AAATGGGAATTCGGTACGTG GTGGTGTCGAATCAACATGG 112A2C06 AUBES3111 534 1072 12 50.1 CAGAAATACACCCTGGAGCTG CCCATGGCTCAGCTTGTAA 013A2A03 AUBES2497 213 318 12 50.9 CACCTGACTACATGCACACG CTCCATCATCCTTCCACAGA 046B2C11 AUBES3513 113 460 12 52.2 GCCTATTTAGATTGGCCTTGG CAGAGGTTCAGCTCAGCAGT 110A1A08 AUBES5130 1623 340 12 52.9 TAAACCGTTGTTCCCTCACC CATGGATCCTCCTGCCTCTA 088A1C05 AUBES3076 216 727 12 53.2 GGAGCAATGCAAACGAAATC CAGGCTGGGCTAAGTCTGTT 029B1G01 AUBES4843 1619 350 12 53.3 ACATAGCCTGCCTCTTCACC TCCAAAAATGCCTTTTGTCTG 075B1E02 AUBES3345 169 715 12 61.8 CCGCACCTTAGTAGCAGTGT TGGGCATTTTGATGAAACCT 106A1D05 AUBES3546 441 338 12 64.6 CCACTGCATGGTCAAGTTTT CATGGACAGCTCATTGCCTA 056A1G08 AUBES3469 1800 379 12 66.7 TGGTCGGAAGGTGTTCCTAT CGACGCAGCATTCTGTAAAA 061A2F02 AUBES4066 492 220 12 67.9 CGCTATTTGAGTGGCCAAAG TGGCCCAGTGAAAAACTCTT 084B2G01 AUBES3416 441 338 12 69.0 CTGCAGGTTGCTGAGACTGT AGGTGCGCATAGACACACTG 028A1A07 AUBES3470 1800 379 12 71.6 GCACGCTCATGTGAAAACAC TCACACTGTCCGACGTGACT 044A1B01 AUBES1430 82 536 12 72.2 GGTTCTGTGTGCCTGTCAGA TCAAGCACTGGTAAAGAACTGG 032A2C10 AUBES3497 596 418 12 72.8 AGGGTGTGAACGACTGTGTTT GAATTCATCGAGGTGCCAAT 056A1A10 AUBES5160 1804 269 12 74.3 TGGAGTGGGTGTTTGTCTTG CATGGGAAGTCAGGTGAGGT 107B2C09 AUBES3496 596 418 12 76.4 CGTCTCCGAGAAACACCGTA CCATTAGGTGGCGGTAACAC 040B1H03 AUBES5222 2302 181 12 77.6 GGAAAAGCACAATGCTGTGA GTCACATGGGTGAAAGGTGTT 058A1D05 AUBES1178 1664 269 12 78.6 TCCGGCCAGCGCCATAGACG TCCTGGTCAGGGTTGTGGTG 011A1B12 AUBES5175 1932 176 12 83.2 GTTATCGCCCTGTTCGTGAC CGTGTCAGGACCTCATCAAC 034B2E03 AUBES3989 2051 413 12 85.5 GATCCGAGAGAGGGAAAAGC TAGCGCTGGAGCTAACACTG 024B2A01 AUBES5392 3427 91 12 86.3 CGAGGAACAATTCGGGTCTT TCCCTGCCACTTCAGAAGATA 092A2C01 AUBES4839 1552 252 12 87.2 AGGCTAAATTCGAGCCAACA ACAGGAACAATGCACCATCC 083A2F06 AUBES5350 3248 132 12 87.3 TAACCTGGTCCCAGATTTGC TTGAGTAGTAATGTTGAGTAATGCTGA 008B2F07 AUBES5007 1126 331 12 88.9 CTGGGAATTGTGATGTTCCA TGTGAGGCGTTTCATTTTGA 052B2A03 AUBES4838 1552 252 12 94.5 ATTCGAGCCAACAAGACCTG GGTCAGCGGACAAACAAGT 003A2C05 AUBES4983 971 338 13 0.0 CCGGTTCCTTCAGTTCTTTTC GCCCGTTTCAAGTTTCTTCA 022A1B03 AUBES5323 2849 413 13 11.7 ATTCTTGGAACTCCCCTGGA TGGACGGATTACACGGTCTT 065A2F08 106 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5384 3399 211 13 11.7 TACAGGTGAGGGGGTGCTTA CATATTAACCAAGGGTGCCAAT 046B1A12 AUBES1959 singleton 13 15.7 GCAGCTGCCTATCTGCATTT GCGATGTTCTTCTGGGTTTC 022B2B02 AUBES1723 3335 309 13 16.1 CGGACGCTCCACATAATCTA CATGAGCCTGACACTGGAGA 020A1C03 AUBES4017 1221 313 13 16.6 GCCGTCTCTGGTTTTATTGC AGCAAGACCACATGTGCAAG 040A2H02 AUBES4759 2673 266 13 16.6 GGACGTTTTTCAGTGCTGGT CACCCTTGAGTGGCTTATCG 066A1E05 AUBES2039 1221 313 13 17.4 CGTTTTCCGTCATTAGACGA TCGCCAACCCTCTCTTTTTA 038B1H03 AUBES2228 172 406 13 17.6 CCTGTTTGTTTACCTCATCGAA AGGGCTCTGAAAGAGTGCAA 042A1D05 AUBES5415 3544 162 13 18.1 GATGACGACGCTCTGGAAAT GGAATGAAAGACAGAGCGAGA 035A1G08 AUBES4885 741 277 13 18.5 AGTGGGTGGTGCAAAAGTTC TTTGGTGGAAGAGTTTGTGG 043A1E08 AUBES2791 700 545 13 20.6 CCTGTGGCTAGATAAACAGAGGT GGGTTTCAGCAAGAACAACAG 054B1F01 AUBES3311 700 545 13 20.6 CCTGTGGCTAGATAAACAGAGGT GGGTTTCAGCAAGAACAACAG 054B1F01 AUBES4517 416 320 13 32.3 ATCCAGCGCATCCCATTAT GATTTTACGCCTGCGTCCT 091A2D06 AUBES4516 416 320 13 34.2 CGATATCATCGTCGCAAATTC AAATGCCGAATGACAGTTCC 010A2H04 AUBES1567 992 318 13 36.0 GAGGAATGTCGTGATCTTTGC GGCATTTCATTTCAGGACTCA 022A1F04 AUBES5174 1906 291 13 39.1 CCCGTGACTTCCTTTAATGC TGCTCGAGTCTGCAGTCAGTA 014A2H07 AUBES2071 2822 306 13 41.4 TTTCCCCTGCTTTCATCATT TTGCAGCATCTGGAAAATGT 039B1G06 AUBES2775 631 524 13 43.4 GCCATTGGTTCTGCTAATGC TAAAGGCAGCGAGCGTTAAG 053B1E12 AUBES2795 980 374 13 44.2 GCAGCGAGCGTTAAGAGAAT CAGTACGCCATTGTTCTGCT 002B1E07 AUBES2908 929 538 13 46.0 ATAAGGTGGCAGTGCTGGTC GCGAGGAAATAGCAGAGGAA 045B2F11 AUBES3089 199 683 13 46.7 CCCAAAAAGGCTCTCTGTAGAA AGTAGGAAAGCAACGCTGGA 051B1F04 AUBES1794 singleton 13 46.8 CTGCATCAAACCAGCAAAGA AGACACCCTTATCCACAGCAA 033B1H10 AUBES3555 401 318 13 47.8 CATGGGTTCATAATTATTGGTTCA TGTGCTTTCACACACACTCG 022A1C03 AUBES3702 199 683 13 47.9 GCACATGACCAGAGCACATT GAAGGTTTTTGCCAAACTGC 004B1D10 AUBES2609 199 683 13 47.9 GCACATGACCAGAGCACATT AGGTTTTTGCCAAACTGCAC 014A1A07 AUBES3110 59 1071 13 48.2 GTTTCTTTCTCCCCGAGCTT GGCTTTCAGAGTCGGAAGTG 058A2H03 AUBES1887 929 538 13 48.3 CATGGAAGGAGTCTCCAGTG CAGCACAATGCAGTTTTGAA 029A2C07 AUBES3734 59 1071 13 48.5 GTTTCTTTCTCCCCGAGCTT GGCTTTCAGAGTCGGAAGTG 074A1G06 AUBES3486 401 318 13 48.7 AGGCTTCGTGCAGACACAC TGGTGCCGGAGAACTTTAAC 082A2E03 AUBES4007 1131 252 13 48.9 GAGGATGAGGACTGGCAGAG GAGGTTGCACACACACACATC 075B2E04 107 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4082 773 311 13 49.0 GGACACACTGGGCTGAAAAA CATGGCTGACAAGCAAATGT 021A2B03 AUBES5281 2575 328 13 49.3 TAGGCATCCATCCATCCATT TGGAAACTGTGGCCAAAAA 072B2E08 AUBES3060 32 555 13 49.6 CATCCAATCAGAGAGCAGCA GCGGTCACAGTTGTGCTTAT 102A2A03 AUBES3733 9 2006 13 49.6 TAGCTGCTGGCACTTCACAG GTGGCCAAGGTTGTCTCATC 073B1D11 AUBES3393 79 281 13 49.6 GTCCCCACAGCACAGAGTTT TGGATGTGTCGAATACTTCCTG 105B2F05 AUBES3149 199 683 13 49.6 TGTGAACCTCTAGGATAAGAGTCA CTTCAGGGGTTTCCTCCAGT 054A2G09 AUBES3557 738 304 13 49.6 CAGTGGCATCACGCATTACT TTGTGGTGGAATAGGGCTAGA 068B2H03 AUBES4907 257 502 13 49.7 ACAGCTTTCATGGGCTGAAT TGTGTTCACACCCATTTCTGA 068B2F08 AUBES3439 652 404 13 49.7 GCTGAAATGCGTCACATGAT GGGTTAGGGCTGCTTGATTA 053A2C06 AUBES2761 344 570 13 49.7 GTGGCCAAGGTTGTCTCATC TTTAGCTGCTGGCACTTCAC 052A2F09 AUBES3392 79 281 13 49.7 GCCAAAGCCTTGAGCTACTG TGCTACTGTTTACTGCTCTTTTGG 105A2B07 AUBES4622 2163 245 13 49.7 CCGTCCTGGTATGAATCTCC CACACAAAATCTCGGCACAC 072B1B12 AUBES3678 59 1071 13 49.8 TGAATTTGCACTGATGGTTCA TCCCCTCATTGTATAACCTCAA 035B1F04 AUBES2637 59 1071 13 49.8 TGCACAGAGGCAAAATTACG GACCAAAGGTTCCCACAAAG 005A1C12 AUBES3476 251 257 13 49.8 CCAGTGCCAGTGCTTTGTAA TCCTTCACCAGCCTTTCTTG 062A2E09 AUBES2422 929 538 13 49.8 TGCTGGTCTGCAGTGGAATA GGGAAGGCCTTATAGAGTTGC 045B2F11 AUBES5087 1493 304 13 49.8 TTTCTTGCCACTGTCACCAC CCTGACCATCACACCCTTATG 050A1H07 AUBES2045 2462 191 13 49.9 CGGCTGATGCAGACATAGTG ATGTGCCAGGCCATGTTTAT 038B2H09 AUBES5411 3536 193 13 50.3 CTTCCTGTCCACCAGAGAGC ATTCTCCTGGTGCTCCCAAT 002B2C02 AUBES3440 652 404 13 50.4 GGAATGAAAACGCTCAAACG GATGCTCGGCTTGGATAGAG 104B1G10 AUBES2704 92 369 13 51.1 TTATGCTGTGTGTTTGCGTGT CCCAGCATGAAATAAAAGACC 027B1G06 AUBES4464 932 184 13 51.3 CGTGCTTGTGTTTGTCTGCT ACCCAATTTGTCAGCGCTAT 051B1F10 AUBES2864 92 369 13 51.5 TTCAATCCCCAGGAAGAAGA GGACACAACAACTCGGATGA 074A2H07 AUBES4918 417 316 13 54.0 TCAATCACTCTCTTGATCCTTTCA GCCAATGTAAAGTTGGCATGT 051B1F11 AUBES4362 738 304 13 54.6 TTGCACCACAGTGCTATTGA TCCCTTGGTATCTTCATTTTCTG 056A1F09 AUBES4006 1131 252 13 55.1 CACGGGCACAGAAAGGTATT TGGTGTTTGCTTGATTTTGC 084B1F03 AUBES1594 3393 303 13 56.8 AAATTAGGCCACGTGTAGGG CCCTCAGTGTTGTGAACCTGT 020B1E04 AUBES2425 3455 213 13 56.8 CAGGTGGATGGATTGTGTTG GCTCAGTATCTCTGCGCATTT 040A1B02 AUBES3030 1303 446 13 61.1 ATCCCAGTCCCGATACACAC TGGCATGAAACAGCTTATGG 054B2A08 108 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5058 1358 365 13 61.3 GCCCAATATGTTTCGACCAA CGAGCTCTCTTCTGAGTCGTC 053A2F12 AUBES2924 1116 497 13 61.9 TCCTTCACTTGACAGCCTCT TTCCCTACCGCAAATTCTTG 045A2D05 AUBES4761 2683 240 13 64.4 TCACTCAGGCTTGACTGTCCT TCCGGTCATGTGTTTTATCG 086B1A01 AUBES5329 3010 381 13 68.0 GCACCAGACAGATGTCCTCA GAAATGTCACTGGTGCTGCT 051B2H03 AUBES1331 2881 171 13 71.1 AGCATCAGCATGGACCTCAG TGCACAGTTAAATCATCACCAGC 004A2H08 AUBES1856 2410 683 13 74.7 GCTGGTTAAGGCACAAATTGA CCCATCCCACTTTTAAATCC 036B2E03 AUBES1896 634 353 13 75.9 TTCATTCAGCTTTGCGTCAT TGGTTTACTTGGGACCATCTT 032B1H04 AUBES2158 634 353 13 77.9 CCCCACTGAAACTATAGGAACG AAACCCACATCTTCATTCTGC 023B2H06 AUBES4264 1354 602 13 81.2 TGTCACACTGGTGTCAAGAGC ACGGTCTTTGGATGTTGGAC 104B2G03 AUBES2538 36 526 13 83.1 CCTCAGACTCGTGCTCCTCT TCAAATTTCAAACGTTGACCA 049A2E08 AUBES5015 1171 296 13 84.6 CGATAGGAGCTTGCTGTTGA CTCCACCTCCCGAAAACAT 075A2F08 AUBES2678 36 526 13 84.8 ATAAAGTTTCCGACCGCATC TCTTCACGCTCTTCTCTTTGC 004A1H08 AUBES2977 36 526 13 84.8 CTGTTCTTTTGCAGCAGCTC AAGCACCATTAAGGCAGGTG 083A1F10 AUBES4150 1246 572 13 88.7 ACAAGGAAAAGCTCAATCGAA GCAAACATCAGGAAATGAAGG 021A2F08 AUBES4149 1246 572 13 90.3 CGCCTACCAAATTCACCTTC CCTACCCAAAACATAGCCACA 067B1C03 AUBES5381 3383 304 13 90.8 TCATGCCACTGCTTCGAATA CAGCCCATAAAGAGGTGCAT 072A1C04 AUBES5240 2391 431 13 92.1 GCCATTTCCTCCTCATCGT TTGGTGTGTTTTTGGCTTCA 107B1A03 AUBES4118 1534 435 13 94.7 CCGTGGAGACTCCTTTCAGA GAAGTCCACTTCAGCCATGT 067B1D09 AUBES4397 1891 331 13 100.7 CCATTAATGAGCCGCTATCC TGGTTATCCAACTCTGCTTGTG 107A2B05 AUBES4820 3124 216 13 101.5 TCAAACGAACACAAGGATGC TATGGCTCCTGATGTGTGGA 046A2H09 AUBES2510 singleton 13 105.1 ACGCATGACACACCAGTACA CATTGCCGGTTTATCGATCT 048A2C11 AUBES2526 singleton 13 109.7 TGACATCCGTTTTGCTTTTG TGTGTACATGCCCATGTGAGT 048B2F10 AUBES4604 1989 193 14 7.1 CTCACGGATGGAGCTGAGAT CCACAAGCTGGAGGTCAGAT 074B1H06 AUBES4605 1989 193 14 8.7 CGGAGGTCATGCACAGTCT TTATCCTTTCAGCGCCTTTC 111B1B04 AUBES2398 3189 315 14 9.6 CGCGGGTAACGATGTTTAGT CTTCTAGAACTTTCCGCACCA 045A1E10 AUBES4467 1115 309 14 15.6 AGGACGTTCTGATCGACTGC TGTTCTTGTTAACCCGAAAAGAG 053A2B05 AUBES5401 3475 249 14 18.0 AAGGTCACGGCTAGGTCAAA TATCCGCTGGGCAATTCTAT 053A2B06 AUBES2433 2327 186 14 19.2 ATATCCGCTGGGCAATTCTA TGTCTCTTCCTGTGGGGAAA 040A1G12 AUBES4783 2883 252 14 27.1 GTGAGCGTTATTCCAGACAGC ACTTCCGGGAGGTAGAGTCC 063B2E07 109 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3987 1955 247 14 30.4 GGAGATGGCCGAATATAAAGC ACGTCTGCTGCTTTGCTTCT 049A1F02 AUBES3932 1344 315 14 48.0 TACTGGCAGCTTCGGGTAAT TGCTGACATTGGAGACTCCTT 072A2F10 AUBES5037 1266 419 14 48.9 CACACAATTTTGCCCTGTGT CTGGGAGTGGACAGAACTGG 079A1H01 AUBES4322 2043 205 14 49.7 TGCTTGTCCGAATGTGTGTT GTGGGGTGAACCTCAAAATG 087B1H01 AUBES2235 156 294 14 50.4 GGCTTACCGAGACTAGGTGCT TGAGGAATAGTCACTACATGGAGAA 042A2B10 AUBES4872 2508 375 14 51.8 GCCCAGATTAATTCACATCACA TTGGAACTCCGCAAATTAGG 096A2B05 AUBES4871 2508 375 14 52.3 AGAATGCATGTCCACACACC TCGTTGCCATTGTATTCACAG 052A2E04 AUBES2352 1324 335 14 52.8 CTCGTGTGGTCCCCAGTAGT CCAAAAAGTAAAACTGGTTTCAGA 047A2B01 AUBES4155 1324 335 14 52.9 TTCCCCTTCCATTTCTCTGTT AACAGATGCTGAAACCAAAGC 093A2E09 AUBES4473 1294 230 14 53.3 AGAAGTTGGGTTTGCACTCG AGCTCCTCACTGGACCAAAG 074A2C09 AUBES4447 3090 230 14 53.6 GGATTCAACAATGAGCAGCA CTGTCTGAAGGGATGCTGTG 033B2A05 AUBES3584 1046 502 14 53.9 ACACCAGTCTGCTCCTCCAG CACTGCAGGCATTCTTCTCA 101A1H04 AUBES4431 3090 230 14 54.1 GGATTCAACAATGAGCAGCA CTGTCTGAAGGGATGCTGTG 033B2A05 AUBES5278 2561 245 14 54.4 TCACAACCCAGCATTACCAA GCTCGTGTCAACAAATTGGA 083B2G06 AUBES3585 1046 502 14 54.8 TGGGGAGAAACACCAAGAAT CACTCCCGGTCAAAAGTTTA 106A1H10 AUBES1340 184 396 14 54.9 TGCTGGTAGGCCCAGTG GTAGGCAGCGTTCCAGC 028B1B06 AUBES2566 1363 940 14 54.9 TCCCATGTAATTGCACCATC CAAAGCCAATGGAAAAGGAA 010A2G07 AUBES2753 197 418 14 54.9 TCAGCCTCCCTCATAACCAC GCACACAGGGTTGAACTGAC 046A2B03 AUBES5034 1241 222 14 54.9 CTGCCACATGGTCTGTCTTC CTCAGTGAGTCCCTCGCTTT 102A2C10 AUBES4811 3086 193 14 55.1 GTTGGATGAGGGTCAGCTTC GGATCAAGCTGTGGAGCACT 001B1B11 AUBES1166 345 589 14 55.1 ATACAGGGCTCGCTTCACAG CTGGGATTACTTGCTCACTGG 008A2E03 AUBES4430 3090 230 14 55.4 ACATCCGAGATGAGTCAAACTT GAGGGAATGACAGAGGCAAC 006B1D03 AUBES5325 2885 362 14 55.5 CATGTGTGGCACTGCATCTT AACATTCTCCTCGGCTGTGT 101A1H11 AUBES4154 1324 335 14 56.0 CGATCGAATTGTCTGTCACG CCTATGAAGGGAGCCAGGAT 053A2G09 AUBES5324 2871 316 14 56.4 CCTGCACCGGTACCTTCTTA CGGCTTGTAGTTTGTTTGATG 104A1D08 AUBES5399 3472 151 14 57.7 CAGAATACGGCCCAGACATT CAGCCTTTCTGGAGCTTTGT 087B2C09 AUBES3618 175 274 14 57.7 CCACTGTTGTGCCAAAGAGA TTGAGACCATCCAAGAATGC 034B2B06 AUBES3617 175 274 14 57.9 CCACTGTTGTGCCAAAGAGA CCATCCTGGGTGATTCTTACA 082A2D10 AUBES5150 1709 254 14 58.4 AGTCCAGTAGGTGGCGGTAA GGAAAGCTGCGTCATTCCTA 040A2D03 110 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3435 595 394 14 58.6 ACATCCCCCTGGCTCTTAAT TGGGAATTGGAGTAGAACAGG 095B2G04 AUBES2079 442 915 14 58.8 TCCACAACCCTAAATGTGGAA CAGCCTGAAGAGACAAAACG 039B2G10 AUBES3214 55 614 14 58.9 CCGCACCAAACTAACAGACA TGAGTGGGCGCTATGATAAG 105B2C09 AUBES3616 156 294 14 58.9 GGTGGCAGCTGGTTAATATTGT AGCCTTGCTCTTTAGCATCG 054B1B09 AUBES3615 156 294 14 58.9 TGGTGATGAACATGAAACAGAAG TTTTGGCCATAATCATGCAG 031B2A07 AUBES3254 336 291 14 58.9 AACGCATCCTCATGCAGTAA TGACCTTGAAAATGCTGAAAAG 084B1D06 AUBES2456 526 386 14 59.8 CCAATCAGAGCAAGAAGCAA GTGCCGAGTTGCTGACTACA 040B1F10 AUBES1112 singleton 14 59.9 ACCTGGAGCTGCTCAGCGTG CTTGGCACTTCACCCGCCAG AUBES1855 1424 487 14 60.5 CTTGCCATTTCCCTGCATAC GGGAGCAGACCAGGTAGACA 036B2C08 AUBES3436 595 394 14 61.6 GCGAGGGCAAGGAGTATCT TGATGCACCAGAGAACCAGA 067B1F10 AUBES3426 526 386 14 61.6 TCACCCTGACATTACCTGGTT CATCATGCTTCTGCAACTTCA 069A1D11 AUBES3549 526 386 14 62.9 TATGTGCAAGCAGCCTTCAG CCATGAACCCATGAACTTCC 005A2C12 AUBES1922 526 386 14 63.3 GGGAAAATGCACGAGAGAGA CCATGAACCCATGAACTTCC 036A2C11 AUBES3329 191 695 14 64.5 GACTGGGTGTAAACAGAATGACC GCCATCAGCCATTCATGTAG 104A1E06 AUBES5339 3211 272 14 65.9 AAGCCTTTTGTTCTGTTCCA GAATGCTATTGCCAGCAGGT 065A2G12 AUBES4982 944 438 14 70.2 CGCATCAACTTTCAGGACAA GCACACTTCTGGTTGCATTT 073A1D11 AUBES3640 710 340 14 71.0 GTGCCACACATTGAGGAAAG AATGCATGCAATGTCCAAGA 073B2C12 AUBES2767 446 536 14 71.5 AGGAGTAGGCGTCTCCAGTG TTGACCATTGTTTCCAGCAA 002B1B05 AUBES4455 1700 255 14 73.2 CCATGTGCTGAGTATGGCATT GGAGCCTCGAAGTTGATGTT 103A2G11 AUBES1965 704 524 14 74.3 TATGGCCAAGGCCGATGTAT CCCAACAAATGGCAGGTACT 028B1B01 AUBES4361 710 340 14 75.3 GCTCAAAAGGTGAGGACTGC AGATCGGGTTTCTGTCTGGA 034B2B05 AUBES3957 704 524 14 76.0 ACCTGATTGGCCCTGAGAC ACCCTCGGACATATCCATCG 083A1C12 AUBES1529 1671 279 14 77.0 TGCATGCATATCCTCGTAAA CCTTGGCAGAAGTCTGTGTG 030B2C04 AUBES1575 1240 276 14 80.1 GCCTTCACACGATGTCAAAA TGCATGGGGAATCTTTTCAT 028A1C07 AUBES3581 1033 245 15 0.0 CTGGACAGTTGAAGCATGGA GTCGATGAAGGAGGGAAACA 060B2D05 AUBES2891 514 502 15 2.8 GTGACCGCAGTGAGTACAGC CCCCGAGACCCAGTAAGAAT 077B1E08 AUBES3541 140 726 15 4.6 TGCAGACAGGGAGGAGAAAT CCTCGTCCTGCAAAGTATCC 096A1H10 AUBES1775 697 220 15 5.2 TGAGGCAATGTTTGAGCAAT CAACCCAAAAACAAACAAAACA 034B2A09 AUBES3304 584 347 15 6.5 CAGTGGAAGCCCCATAATGT CTTGCCAGAACAGCTGGATA 069B2A03 111 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3873 869 502 15 6.5 ATGGCCAGCGGTTCATATAC CGTTCATTTTGCTGAACGAG 032B2C11 AUBES3875 869 502 15 6.5 GGCCAGCGGTTTCTTATACA CGTTCATTTTGCTGAACGAG 075A2D06 AUBES2999 514 502 15 6.5 GTGACCGCAGTGAGTACAGC CCCCGAGACCCAGTAAGAAT 077B1E08 AUBES1906 363 328 15 8.3 GCACCATAACCCTCCTGCTA AGCGATGAGCTGGTGTTTTT 036B1A04 AUBES3407 363 328 15 8.4 GCCTCAAAGCCTTCTGTTTG GATTGTGCTCCAGTCATCCA 069B2F02 AUBES3132 890 863 15 9.0 CCCATGCACGTGATATTGAT TGGTCATGACTCACTGGACTG 108B2F02 AUBES2357 1492 228 15 9.9 CGTTGAGACGTCTTTGTTACG TCGTTCAGCATAGTGTCTGC 047A2F10 AUBES5403 3483 240 15 11.4 CGAACGTCTTTGAGTTGTTGT TGGAAATCTCCTTTTCCAGGT 052B1G11 AUBES2479 1097 220 15 11.4 TGTGGAAATGCCTGTTTCCT CCATTGTCTCAAACGCTCCT 046A1F05 AUBES2404 179 340 15 15.2 CACAGCAAACTGCCTACGAT GATCAGCTCTCAGTAAGCGACA 045A2B10 AUBES3620 208 348 15 17.5 GGAGGGGAAATGACATCATACT CCACAAAGTTTTGGCACAGA 006A1G05 AUBES1737 958 553 15 20.7 TGCTCAGCACTGAAATCACA AAAGCAAGCAGTCATCACGA 031A1F10 AUBES3040 958 553 15 22.0 CCCAACCTTGCCTTATGCT AGGGGGTAGGGTTAGTGTGG 062B2B11 AUBES3031 86 288 15 25.7 TTCCGATCAGTAAACCAGCA CACCCTGTCCAGGATGTACC 055A1E05 AUBES3937 76 463 15 28.0 GGCCGATGTCACTCTGAATA ATCAGAGTTTGCGGAGATGG 095A1A04 AUBES5310 2728 173 15 29.4 CACGCCAGCTCTCACTACAG GTGGACCTGGGAATAACAGC 105A2C07 AUBES2861 76 463 15 30.1 TGGGGAAGGTCTGTAGCATT AGTGAGTATGCCCCAAGAGG 053B2B07 AUBES2522 1152 264 15 30.8 TGAGGATGTGATGGATTTGC CCCAGCCTTAAAGCCTCAAT 048B2A06 AUBES3131 214 580 15 31.0 GCCTGAGGTTGCTTCAAAAG GCTCTGTGCAATTTGTGTGC 102B1A02 AUBES3728 34 490 15 31.3 ACGGGGTCAGTCAGATCAGT GGATGCAGGTGGTTACGAGT 064A1B10 AUBES3431 588 276 15 31.5 GGCCGTTGACGTTACCTTTA GTCCCAACCTGTGAGCTGTT 045A1B01 AUBES5038 1268 230 15 31.9 TAACCAGGGTCAGAGCAAGG TCGTCTCAGCTGCAAAAACT 080A1H12 AUBES3619 208 348 15 32.1 GGACGACGTCTCCTTCAGAT CTGTCAACCAGTGGAGTCAGA 110A2C07 AUBES4931 567 315 15 32.1 CCACTGCCCTCACTAGCAG TGAGGTTCACGTGCTGATTC 095A2E09 AUBES2570 2 403 15 32.2 CCATGCTGCAACATATCCAG GCTCATGTTGGAGACGTGAA 035A2C05 AUBES3072 34 490 15 32.4 CGGGGTCAGTCAGATCAGTT AGAGGATGCAGGTGGTTACG 030A1A03 AUBES2725 118 440 15 32.4 GACTTGCCCGAGTGTCAGTA AAAGCGCAAATTGCTAAGGA 013B1F03 AUBES3938 76 463 15 32.8 GTGGTGGGGAAGGTCTGTAG AGTGAGTATGCCCCAAGAGG 061A1D11 AUBES3965 1602 289 15 33.0 AGCCTGGCTCTACACTGGAC GAACCTTTCACCGCAATGTC 086A2B10 112 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2903 671 541 15 33.2 TGTGCCCTATGATGGGTTG ACCTTCTGCCCATTGTTCAC 043B2D09 AUBES3506 28 474 15 33.5 TCTTCCCTGTTTGAGGCACT CCAGGAAACAAAAACGATGG 054A2E04 AUBES3071 214 580 15 33.6 TTGTTGGGAAACACTTCAACAG AGCCTATCCCAGGGGACTC 020B1A09 AUBES4240 924 294 15 33.6 GCAAAGACCCCACTTCTGAG ATCGGCAATTAACGGCACTA 110B2H01 AUBES2943 256 511 15 33.9 AGCTCCAGGGCTTCTGATG TATGAGCCAAGCACTGCAAG 006A1D11 AUBES1912 323 585 15 33.9 GAGCAGTGGCCATTTTGTTT GAAATAGATTGGGGGAAGGA 036B1D01 AUBES3265 671 541 15 33.9 TGTGCCCTATGATGGGTTG ACCTTCTGCCCATTGTTCAC 079A1F03 AUBES4124 818 338 15 33.9 AGCAACAAATGGGGTGACTC TGTCTGGACAAAGGATGTTCA 095A1F07 AUBES2752 187 529 15 33.9 GCCAGCACCCTTACAGACAT CCTCTTTAGGCTCCAGCAGA 047B1C07 AUBES2391 588 276 15 33.9 CGACTGACAGCGGAGATTT GTCCCAACCTGTGAGCTGTT 045A1B01 AUBES3354 116 621 15 34.0 ATCGCCCCAACTTTCGTTTA CTCACTCTCGCCACGTGATA 063B1C11 AUBES2599 116 621 15 34.0 AAGGGCCTTTGTCACATGAT CACTCCAGTTTCTGGCCAAC 022A2D11 AUBES3100 104 852 15 34.1 CCTGAAATCTTTCCTGTTTTTG GCGGTCATGTTACCTTTGGT 098B1D06 AUBES4621 2156 237 15 34.1 TGTTATGTGCCTTCCAGCAG GAATGTGCAAATGCAAGCAC 014B2H03 AUBES3191 116 621 15 34.4 CCCCATACAAGGTAAAGTGCT CAGAGGCAGTCAGCTTTTCA 031B1B09 AUBES2622 104 852 15 34.4 TGGTCGAGGTCATTTCTCATC CAGTAGTTTTAGGCAGCACGTT 014B1B09 AUBES2581 214 580 15 34.6 TGTGTAATGGGATTCTCATGC CCTCGTGTGGCTAAATGTGA 066A2G10 AUBES2469 116 621 15 34.8 GACTTGTGCCATGGTTGTTG TTGATGAGCCTGTTCCCACT 040B2F06 AUBES3432 588 276 15 35.0 TCTGTCTCAAATGCTCGGTCT GTGCCAAATGTTCAAAGCAA 068A2H05 AUBES3736 818 338 15 35.1 TTTCACAGCTTTCTCTGCTCA ACCCCACTGTGAAATCCTGT 076B2B08 AUBES3158 104 852 15 35.4 CGGTGATGGAAATGTACACG CAGTACGGGGAAGTGTTTTGA 082A2C10 AUBES1728 657 424 15 35.7 TTGGCCTCTAAATTTGTGCTC GCATGAATGAGCTCACAAGC 023B1C08 AUBES1733 104 852 15 35.8 CGCATCCCAGCGTACAATTA AACTGACCGGAACTACCTGTG 027A2E05 AUBES2881 323 585 15 36.4 TGTTTGCCTTTTGAACCAGA AAGGCAAATTGATGGATGGA 058B1E12 AUBES1936 1471 215 15 36.7 CAGTGGGGCTCTGTCAAACT GTGCACTATGGGGATTTCGT 036A1D09 AUBES3507 28 474 15 37.4 TGGCTTGAAAAAGCCAACAT CAGCCCGAAGGACTGTTTAG 061A1B09 AUBES5265 2515 370 15 40.0 GCATTATTTGTGCGAACGTG CGGTAAAAATGCCAGGTGAT 102B1A01 AUBES4848 1719 419 15 40.9 GTTGGCATTGTACACGTTCG CTTCGACGGTGAGGACTTTC 009A2E07 AUBES4208 648 338 15 41.8 GCATGGAGACACTGATGGAC TGCAAATTACAAGGTGATTCCA 069A2D05 113 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5379 3374 215 15 42.7 TGTCCGCATCTCATGTCATC ATTGTGCTTCATGTCGCTTG 087A1C06 AUBES3314 717 472 15 42.9 TTTGGCCCAATAATTTTGCT CGTTTCCACTTCCGCACTAT 112A2A07 AUBES1571 singleton 15 44.1 CACACACACATTCCTTTCCAA GAGGGGTTCAGAGCATGTTT 024B1C02 AUBES3835 1919 311 15 44.7 GTTGGCATTGTACACGTTCG CTTTTCACGGAGCCAGATGT 111B2D10 AUBES5363 3293 250 15 46.0 CGTAGTTTGGTTTTCGTTATAGG CGATATGAACGCGAACATTAAA 013B1A01 AUBES2783 950 666 15 48.6 GCCATACTGCCCACCCTATC ACATGGTGACGGTGATCTGA 076B2G02 AUBES2831 717 472 15 49.2 CCCTCATTGCTTGTTCTTCG CCAGTTCCTCCTGGTGCTAC 009B2C01 AUBES5215 2263 218 15 49.4 ATGGGACATGATTGTTTGTTTG GATGGAAGGAGACCTGGACA 038B2H05 AUBES4030 1349 345 15 51.6 CAGTTGCCTCACACTTGAGC CTCTGTACCGCTGCATCTGA 104A2F05 AUBES4739 2350 225 15 57.6 AAACGTTTCCTGTTCAACGTC GGACTAGTTATGGGGCAGCA 079B1A10 AUBES5223 2316 178 15 64.1 CGATCCTCCAGGGAACTACA GCTGTTTCCAGTTGGTACATGA 108B2B03 AUBES2344 singleton 15 69.5 CCTGCAGAAACACAAGCAGA CTCTTGCTGATGGTTCCTTTC 047A1D12 AUBES4329 2146 298 15 83.2 TGGCTCAGCATGTTTTTCAG GCTTCAGCACTGTGAGATCATT 011A2F06 AUBES5358 3282 225 15 93.4 TCGCCAATATATTACAGCATGAA TGCTGGATCAAGTGCTATTCT 032A1B05 AUBES1869 singleton 15 93.7 TTGCTACAGCTGAGGCATGT GAGCCGGGTTACACCTAACA 025A1E08 AUBES2910 1100 397 16 0.0 AAAAGAAGCGCCAGACTCAC TCAAAGGAAGGTCTCACAAGG 054B1E02 AUBES2679 1100 397 16 11.4 CACGTCCCACATCTTTCACA TGAATCAGTGGGCATTCAGA 004B2B07 AUBES5243 2400 291 16 12.9 AGTTTTCGGTGTATCGCTTG AGGCATGTGGCTCCAATTA 057A2D04 AUBES3586 1062 480 16 14.3 TGCGCTCAAGAGTTGCACTA TGGAGATGCATTGAGGAGAA 096A2G09 AUBES4305 1914 387 16 15.8 TTCTGTGGAAGCCCTGAACT AGGTTCCACTGTGACCCTGA 054B1F03 AUBES3587 1062 480 16 18.0 CGCCAATCATTCTGGAGTTT CACTTGGCAGCATCAAGAAA 075A1E10 AUBES4629 1956 489 16 18.1 AGGGGGAAGCGACATGATTA ATGTTTGCCGATTACAAATGG 096A1G08 AUBES4799 3014 196 16 18.1 CAGTACTCGTCGTTGGCATC CGGGTGGAGAGAGATTGTGT 104A1B10 AUBES4628 1956 489 16 19.8 CCTGGAAAAGTTTGGGAACC TCTGCGTGCTTTTGTTTTGT 058A2E09 AUBES4445 2920 269 16 24.4 CACAGCGAGATTCCAGATCA GACTTGGGACACGCCTTTAAT 055A2B08 AUBES4429 2920 269 16 24.4 GCCATCCCAAACGTGTACTT GCCAAAGATCGAAGGTGAAA 055A2B08 AUBES4428 2920 269 16 24.4 CGACAAAAACAAATCCGATG GGAAACGTGCTAACCGCTAA 038A2D02 AUBES2072 singleton 16 30.5 CCGAATTTGCATCTTTCACC GCGCACACACAAAGAGGTAG 039B1H02 AUBES4835 3193 132 16 37.1 ACTGCAAATTGCTGCGGTAT ATTTGGGTGGTGCTTCATTC 004A2G05 114 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2431 singleton 16 45.1 GTCACAAAGTGTCGGTCCAG GGGCGAGTGTGAAAAAGTGT 040A1E02 AUBES2488 singleton 16 46.0 TCCTGAAGGGAAATATTCCTGA CCGAATGTAAATCGCATCAT 046B1B06 AUBES4314 1947 198 16 50.4 AGACGGAGCAGAAACAGCAG GCTTGCAATTCTCTCACACC 102B1C11 AUBES1374 1051 306 16 50.6 GAGCTGAGCTGAGCTGC CTCCCGCACAACAGACG 022A1F10 AUBES5375 3354 250 16 50.9 GCCTCCTGACCAATCACAAT TCACGGTGCTCAAGTACTGC 052B2B09 AUBES3014 435 465 16 54.6 CCCAAAAATCAGAAGGCAGA GATGAGACCGTGGCTGAACT 040B1A03 AUBES4818 3116 100 16 60.9 GTCCAGGGCTCGATTATGTC AGGAGAAAACGTATCTGCCAAT 092B1B11 AUBES3366 442 915 16 63.4 GTTTCGGCAGACCAATAGGA CCACCATTGCCGTCTAAAAC 095A2C07 AUBES2749 132 675 16 64.0 CGTTCAGCAATAAAACGGAAT CCAAACTCTGACTGAGAGGACA 023B1A06 AUBES1950 singleton 16 64.2 CTACACCATGAAGGGCCAGT CTGGCCACAGAGCAGAGAG 003B1H09 AUBES2758 332 480 16 64.3 AAGGCATCAGGATGGATCAG TCCCTGCTTGATTTAGCACA 041A1G04 AUBES3562 761 348 16 64.8 AGCTTGTGGTGGCCTAACAG ATCATCCCAGACCCAAAGGT 105B2B07 AUBES5115 1537 161 16 65.7 CTGAGTTTGCTGGATTTTGG TGTGAATGGACCTTCTTGGA 078A2D07 AUBES3915 1079 330 16 66.3 TGACCCTCTACTTCCCTCCTC TGAAGACGGACACTGGAACA 106B1C04 AUBES2620 101 927 16 69.5 TTCACCCTGCCATGTGATTA GGGTGGAGTCTTCCTTTAACC 032A2D10 AUBES3723 435 465 16 70.8 TGCATTCATTGTTGTATCATTCTG AATGTTTTGGGGAAGCACCT 049A1H06 AUBES2611 241 913 16 71.2 GATGGGTGATGAGGGTATGC GGGTTCCTAAAAATGAAGTGGA 070A1A08 AUBES2274 241 913 16 71.6 GGTTGCATTCGATTTTTCCA TGCTCCAAACTGGAGGAGAT 043B1E12 AUBES3783 1813 331 16 71.9 CTCAGTCTTGCAGCATCAGG TTTCTCTTCCTCCTACCCCTCT 077B1D03 AUBES3919 1145 279 16 72.3 GTACACCCTGAAGGGCTTTG CAGAATTCAGAAAGGCTGTGC 008A2A10 AUBES4808 3079 245 16 72.4 CTGTGTGCCTGATTGTCTGC TGTGGCATAGAGTGCTGCAT 021B1E12 AUBES3224 101 927 16 73.1 TTGGTTTATCTGTGGGTAGGAAA TTTCGCAAACTTTCAAGCAC 035A1A12 AUBES2387 153 416 16 73.1 CTGCTACTTTCACCCCGAAA CACCTGCACCAGAATCACAT 044B2A11 AUBES3023 621 441 16 73.2 AAACGGATTTCAAGCAAGAGA GGGGAGGTGTAGTAGGAGGA 051B1C07 AUBES3196 101 927 16 73.2 GCCTCTGGGTGAGCAAAATA ATGGCCAAACAGAGACAGGT 006A2C06 AUBES3362 241 913 16 73.3 CAACCTCATGTGGGGTCAC GAGAGCGTGTTGGACTTGGT 084A2F05 AUBES2069 singleton 16 73.3 AGCTGTGATCGTCCTGAGGT AAGGGGCTTGTGAAGAAAGG 039B1D03 AUBES3861 342 272 16 73.3 CAGATTCAGTGTGATGGTGGA GTACGGGGACAAATGCAATG 034A1G04 AUBES2632 45 1120 16 73.3 GGAGCTCTGTGAAAAGCTGTG GACTTGAATCGCTGGGTGAC 074A1C04 115 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2786 1185 509 16 73.3 CCAAGCACTGAAGGTGCATA ACTGCCCTCTATCACTGTGC 051A1G04 AUBES3368 101 927 16 73.3 CACATGGGTGTTGGATCACT GCTGCTACTGAAAGTGCACAA 074A2D05 AUBES4910 299 228 16 73.3 CATGGACAGTGCACAAAACC AGCCACAACTCGTTCCAAAT 069A1G11 AUBES5360 3285 184 16 73.3 AGCCACCGCTTCCTCTTAAT GACAGGGATAGGCTGGTGTG 057B1F09 AUBES3151 241 913 16 73.4 GAGGATGCAGTGAGGACACC TGTGCACCGAGTGTGTTGTA 111B2G05 AUBES2397 4 348 16 73.4 CTGATTCACCCGAGGAAAAA ATAAAACCACGCAGGAGGAG 045A1D11 AUBES4883 685 215 16 73.5 GCAGGGTGATCACAGATGAA TTGAAAATCTGTGTAGGCATGG 064B2C11 AUBES3348 38 702 16 73.7 ACATGCCTTAACCTCCCACA CGACCTGTCCTTTAGGCAAC 068B2A02 AUBES2594 38 702 16 73.7 GCATAAATGTATGTGCCCTGTC ACCATGGTGAGCAGAAAAGC 018A2A12 AUBES3249 153 416 16 73.7 CGTGTCCAATCCAGTAACCA TGCCCTGATTTGTGTGTGA 040B1D07 AUBES1876 38 702 16 73.7 CCAGCTGAAGTGAGATGGTG GGTGCTGAAGTGCCAAGACT 026A2A04 AUBES3353 6 633 16 74.2 GCCAGGATATCTCGCTTACA GTGCTTCAGCTGCTTCAAGA 070A2A05 AUBES4234 833 411 16 74.2 TGAGCTTGGATCAATGTATTCC TGACCCCTAAATCATCCCCTA 011B1E06 AUBES3219 6 633 16 74.2 CACGGCAGACCTTAGCAATA CAGAGCTGCTGCAACGTAGA 045A1A06 AUBES3190 6 633 16 75.1 CCCTTCCCTATGACCTCCTC GGTTAAGAGGATTTGCATCCAT 059B2A04 AUBES4948 662 277 16 75.3 CATATCCCAGCCCTCAGTGT CACTCCACTTCTGCAGCCTA 053A2B07 AUBES4905 235 240 16 76.2 GACAAACACTTCCTGCTATCAGTT TAAAATTCGGCCTTGGGTCT 109B1A08 AUBES2588 169 715 16 76.5 TGGTTAGTGTGAGGGGTTGC AAACAACGTTGGTCCTGTCC 012A1C10 AUBES3788 430 328 16 77.3 CAACAGAATAAACGGAATCATAAAGA CACATGTTTGGACATGCAGA 021B2C05 AUBES4806 3074 271 16 77.3 GTGTCCCCTGTTACCTGTGC GGGTAGTGGCAGGATCAATG 092B1G12 AUBES3637 644 335 16 77.4 CACCTGAGAGATCATGAAAACA ACACCGGAACTGTCACTGGT 105B2F04 AUBES1886 644 335 16 77.5 GGCTCTTGACCTGAATTGTG GTTGCACTTGATGCAAAGGA 029A1A12 AUBES3112 9 2006 16 78.1 CGCCGCAAATTTGGTTTAAT TTACATCCAGCACGCACAAT 109A1B07 AUBES5022 1199 200 16 79.5 GAGTGCATTACTTTGAACCTTGC CACAAACTGCCAGCAATACAG 051B2E09 AUBES5145 1689 233 16 80.2 TCATGGATGCAAAGGCAATA GCAGCGTCACTTAAGCTCGT 106B2H02 AUBES2978 38 702 16 81.5 GATGCCAAACCCTGTAGAGC GCGTTTGAGCATGTGTTTGT 100B1E07 AUBES2940 160 375 16 82.5 GTTCCTGCCAAATTTCCAAC CTGGGCTATGAGGAACAAGG 012B2H06 AUBES2877 245 426 16 83.2 TGAGATGGGTTGACATGTGG GGAAACAAATGGGGTTATCTCA 033B1A05 AUBES1852 46 335 16 83.5 GTGTTTTGGGGGCAGAACT TCTTTCTTGGGTCATCAATCC 036B1G09 116 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES1096 singleton 16 84.4 GTCAAGCACATGGCATGACC AATCTGCCACTGCTGTTGAG 010A2C11 AUBES4954 694 225 16 87.3 CCCTGCTATTCCGCAGTCTA ATGTTTCGATTAATGAGATGAGG 102A1A11 AUBES5210 2209 240 16 92.4 CCATGGGGTGTACTTATTTTTCA CCTGCAGGACCTTCATGACTA 013B1A07 AUBES4534 1977 233 16 97.5 CATTGCACATCAACAAACCA CACGGAATTCGTGACAAGTG 004B2A08 AUBES4433 900 218 16 102.8 TGACTTTGCATTCTGTCTTTTC TCGACTCGTCCTCTTTTTGG 108B1H01 AUBES4432 900 218 16 103.8 CGAGTCCTCTGGGAAATGAC GCAGGAGCAAACAGACTTGG 033A2F03 AUBES4965 824 252 16 105.9 GTGTTCAAACCCCTGCTCAT ATTGTGTCCTGCTGCTCAAT 004B2H03 AUBES5383 3398 90 16 109.5 CCCTTTGGGACCACTATCAA CGACGGCAGATAAATCCAGT 047A1B11 AUBES4063 445 342 16 111.6 TAACGGTTGTGGGAGGAGAC GGAGTTGGGGAGGTCAGAGT 006A2C12 AUBES4415 2476 279 17 4.1 CGGCTGGATCGTTAAAATGT CGCGTAATGATCCGGAAA 030B1H05 AUBES3213 58 631 17 4.2 GTCTACTGCCAATCCTTAAGAGC GCTCCTGCATCGGTAAAGTC 030B1E04 AUBES3742 790 651 17 4.6 TGGTTTGTACCCATGCTTCA GTGCTCCTGTCACTCACCTG 087B1D01 AUBES1536 1027 448 17 4.6 TCCATGGGGTGTACTTGTCC TGCTGGCTGTAATTTGAACA 029A2A02 AUBES4853 1761 266 17 4.6 CCCACAAAAACACACACACAC AATCTGTCCGTCTGTGATCG 011B2E06 AUBES4969 856 348 17 4.6 AATGGGGCTGTTATTCCAGA TGCTCTTTGGGAATCCTCAC 062A2C09 AUBES2616 885 1133 17 4.7 ACAAGCCTCGTGGTATGCTC GCATTGGAACCTTTGTTTCAG 057B2F02 AUBES3222 885 1133 17 4.7 CCGTTGAATTATTTCACTCACAA TGACCGGAGCTTTATTTATGC 031B1C07 AUBES2974 1650 421 17 4.7 GGTGGGAATACACCCTGGA TGCACATTCTCCTCTTGTGC 002B2F08 AUBES3300 499 455 17 4.9 TCAAGCACCTATTAGTCTGCAA TGGGTAATCTCATCGGACAT 064B2G07 AUBES1550 1938 201 17 5.2 GCGTGGCAATAATATAGATGTCG AAGGAGCTGAGATCTGCTTGG 027A1F04 AUBES4664 386 277 17 5.3 AGAGGGCTCAGTGTTGTTGC CCTCACTCACCTCCACACCT 073A1F06 AUBES4185 231 582 17 5.3 GCCACAGATTCCTCCTGAGA GGACCAGACGTGCTCTTGTT 084A2F12 AUBES5035 1247 242 17 5.3 CACGGCAGACACTCAAAGAG GCGGTTTACCTCGGCTTTA 062A1H05 AUBES3085 13 585 17 5.3 CAGCTTGGCAATTTATGAGG ACTGCGGTGACACAAATGAA 020B1D09 AUBES2604 13 585 17 5.3 TCATTGTCTTTAGACTTGCTTGTAATG ATACCCCAGGGAAATTGGAC 014A1C04 AUBES3168 139 1167 17 5.3 GACGCAGCGGAATAACTGA CATTACGCTCCAGCCAGAGT 107B2D09 AUBES2636 139 1167 17 5.3 CAACGCGTGTATGCATTGTT TGATAAATCCCACACGTTGC 035B2G10 AUBES4663 386 277 17 5.3 TGGAATCCAAGACTTTGTGG TCTGAATCTGGCAAAAGTGC 011A2G06 AUBES2659 393 286 17 5.3 GTGTCAGTGCTTTGCAGCAG GGAATGTGTGCAAAACAAGC 005B1F07 117 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3551 604 323 17 5.3 GCACATGTGACTCTGATCGT GCTGGTTTCCTGAAGGTTTG 109B1C07 AUBES2730 750 445 17 5.3 GAACAGCAGCGCATCAAATA GGTGGACACCAGACACTGAC 021B1B07 AUBES3221 885 1133 17 5.3 CAGCTAACCTGCACAGACCA CACAGCCAACAATGAGTGCT 104A2D04 AUBES2466 1034 309 17 5.3 AGTGGTGTTGGGGGAACAAT TCCCCATCCACCACATAACT 040B2D11 AUBES5083 1453 282 17 5.3 CACTGTTCACCCACAGCAAC GCAAACTGGATGTCTTTGAGAA 032B1B01 AUBES3609 1575 313 17 5.3 GCCTAGACGCCGAAGAATTT AAGTCCAACCTAGATACAATAGGC 013A1F04 AUBES4375 1575 313 17 5.3 ATCCACAAATGATCCGAAGTG CCGAAGCACTAACAACAGCA 008A1B11 AUBES4320 2017 191 17 5.3 GAGAACACGTGCTGAAAAGC TGGCTGTAATTATCCGCTCTC 072B2C03 AUBES4416 2476 279 17 5.3 ATTCCAGCCACAATTCCTGA GCCTTGGACTTGCAGCTACT 112B1F04 AUBES5135 1644 276 17 5.3 GAGTGCCTTCAGGTTGCCTA AACATTTCCCACTGGAGCAC 071B1F09 AUBES3379 9 2006 17 5.3 ACCCTTGTTCTGTGCAGGTC CCTTGTGCAAACACAACAGC 081B2D08 AUBES4896 24 304 17 5.3 CGGTTGCAACACACTCAAAG CCAGCCCACTAGACAAGCAT 096A1C04 AUBES2750 151 585 17 5.3 TTGACAAGGGAAATTCACACA CTCATTTCCACAGTCTCACAGA 062B1B09 AUBES1704 361 196 17 5.3 CAACATTCAGCCAAAAGCAA GGAAAGAATCCCACACCTGA 033B1H02 AUBES4512 394 416 17 5.3 TGCAGTCCATCTCTGGAACA GCAGCTGGAGTTTCATCACA 078B1F09 AUBES2121 726 746 17 5.3 GAGCAGTATTGGGGTCCAGA TCTGTTTGCTGTTAAGTCATTGC 018B1A11 AUBES2605 726 746 17 5.3 CACATCTCATAGCCTCATACATGG TGCATCTGCTTCAGGTGTTC 062A1F10 AUBES4242 1012 306 17 5.3 CGGCCTCTATAGGCGAAATA AGGAGATTTCCTCCCCTCAT 072B1E11 AUBES4009 1150 161 17 5.3 TCATCCCATCAACTCTGCTG GATCCTGGTGGTGTCAGAGG 063A1C02 AUBES3924 1165 208 17 5.3 GCCAAGGACCACTCAGAAAG TCCATTGGTTGAGTTCCAGA 096B1D10 AUBES3779 1449 396 17 5.3 GAGTGTTGCTGGGGTAGCAC GGTGGGCTGTGAAATTCAGT 042A2B12 AUBES5082 1452 338 17 5.3 CTGGCTAAAATGCATGGTGA AGCAGGTCTGATTCGCAACT 031B2C01 AUBES3466 1509 375 17 5.3 GCTCTGTTAATGACCCGTGAA ATGACTGCCGCTCACTGTAA 060B1H03 AUBES2054 1917 167 17 5.3 CAGCCGTGAGGTTCAGTCTA TGACAGCCATGGAATCAGAG 039A2C09 AUBES4402 1988 203 17 5.3 CAGCTCTGAGCAGACACACA CTGTTTCCCTCCTTTCATGC 020B1D12 AUBES4323 2065 201 17 5.3 TTTTCATTTCTCAGGGTCCAG CCCTTTCACTGTCAAGCACA 044A2A06 AUBES2108 2205 188 17 5.3 TCATCGACCAGGACAAACAA GTGGATTGCTCAGGTGTGTG 041B2A11 AUBES5233 2367 281 17 5.3 GGGTCGGTTAAAACACAGGTT AAACAGCCTATAGCATCCTCCA 070A2E10 AUBES4750 2592 235 17 5.3 GAAAGCAAACAAAGGAAAAGG GAGCCTCTCGTCTGAGCACT 049B1F10 118 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2417 singleton 17 5.3 TGCTGTATGCTTCAAAATGAGC CAGTGTTTTCTTGTGATGCTGA 045B2B04 AUBES3347 454 550 17 5.3 CCATGGGATTGTGAAGTTGA CTGCAAGGCCCTTAACACTC 110B2F02 AUBES2955 756 399 17 5.3 ATCATTTCCAGAAGGCCAGA TCGCCGTGTACAGTACCTGA 030A1F09 AUBES3583 1034 309 17 5.3 CATGCTCAAGCAATTTTTGG TCATTTCAATCTTCTGCTTTCTG 061B1A01 AUBES3374 50 1049 17 5.3 CCCCTTTTCCTTGCATTACC TGAAGTGCTGCTTGAATGGT 098A1F11 AUBES2392 2065 201 17 5.3 AACATCCTCAACTCCACAGAA TTGAGCATAACTGTAGGGAAAATG 045A1B05 AUBES3579 987 326 17 5.8 GGGCAGGCCTAATTTCTGTC AGGAGACTTCGTCCGGAAAT 004A1B07 AUBES3947 180 890 17 5.9 GCACACAAGCCTAAACATGC CCCAAACACCATAACAAGGAG 008A1C09 AUBES2567 790 651 17 5.9 TGGCTGATCGGTGTAGAGC TACTGGCAGTGATTGGCTGA 038B2F10 AUBES3454 997 338 17 5.9 GAACAGCTTTCGCTCATTCA CGTGAAAAATTGCCGGTATC 006B1D09 AUBES4403 1988 203 17 5.9 TCAGAAAGCTGGGGAGAAAA GCATCGACCGAATGTAAGTG 075A1B04 AUBES2873 180 890 17 5.9 ACACAAGCCGTAACATGCAG TCCCAAACACCATAACAAGGA 051A1A12 AUBES4279 1668 250 17 6.0 TTTCAGATGACACCAATTTCAA ACAAAATCAGCAGGGGATCA 005B2F10 AUBES3437 604 323 17 6.1 TCACCCATTAACCAGCACAA CTTCCAGAGTCGGTTCCACA 108A1E08 AUBES4884 688 266 17 6.3 CCCATGCACATCAGAACAAC GACCGAGCATGAGTGCTTGT 039A1C05 AUBES4909 289 386 17 6.7 CCCCAAAGCCATAGGACTAC GGCAATTGATTTGCTGCATT 083A1B10 AUBES1982 singleton 17 6.9 AATGACGACCGTGATGACAA TTTCAAGCTTGGTAAGCACCT 022B2G10 AUBES3346 454 550 17 7.1 GACCATGGGATTGTGAAGTTG GCCCTTCTGCAAGGTTCTTA 045A1G03 AUBES1916 219 423 17 7.2 CAGGGCACTGCATAGCATC GGCCCATTTGTATTTCTTCTCA 036B2H10 AUBES2267 1034 309 17 7.8 GTGCAGTGGAGCAGCAATAA CAATGACCAGGAGGGTTTGT 043A2F03 AUBES4369 987 326 17 8.5 CCAGGCCAGAAGTCTTCATT TGCAGCTGGGAGCTCTTATT 007B2A05 AUBES3465 1509 375 17 8.7 AAGCTGAAGAGTGCAAATGAGA TCCTTGAGCTTGTGGAAAGG 011A2B06 AUBES2002 579 479 17 10.3 CGATAACGGGCTTAATCTGC CCTGTTAACCTTTGCGGTTC 028B1F05 AUBES3580 987 326 17 12.1 GCACTGGGCTTCTAGTGGAC GTGGCTTGAAAGTGGGAACT 007A1A04 AUBES2930 1444 531 18 0.0 TGGGGCATTTGTTCCTAAAG GGTGTCACAAGGAGGGAGAA 053A2E12 AUBES5251 2435 205 18 0.6 GTGAGGGTTTGTGTGCGTTA GCGCGCTACATTTACTTCCT 063A2C09 AUBES2067 901 485 18 3.3 GGAGGCCCTAAGCTTCATTT TTTTGATGGGGGACATTGTT 039B1B08 AUBES3449 918 533 18 8.7 GCGAATAAAGCACTGTTTCCA CCATAAGACGACACGCACAT 102A1C09 AUBES3552 918 533 18 8.7 CCCTGCTGATAGGGATTTTG TGAGATTGGATGCAGGTGTG 013B2F08 119 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4933 573 355 18 9.6 TGTAAATGAGCAACGCTGGTA AGCCCAATATGCTGCTTTGT 063B2A09 AUBES5314 2739 271 18 9.7 CGGAAGTCGGTTTTCCAAAT AAACTCAGCAAGTTGCATCG 053B1D12 AUBES5390 3417 225 18 9.7 TCATCCCAAGACAACCTGAAC GACCCCTTTCCCAGATCAAG 088A1D10 AUBES2968 1267 543 18 11.8 GCATCGTGCTGTAATGTTCG AAGTCGTGCCGGCTAGTTC 022A1H06 AUBES4183 168 381 18 12.6 GACTGCAGGACACCCCTTC GCACTTATTGCTCATGCTTCC 109A1B04 AUBES1325 168 381 18 13.5 GTTTAGTCGCATACTGTTGCTCAC AAGAATCACAGCCCAGATGC 027A2B01 AUBES2650 168 381 18 15.3 CGTGTAAGTTGCGGGCTAGT GGCAAGCAGAAATCTCCAAA 022A1G08 AUBES5345 3231 184 18 18.7 GCAGTGAGCTGATGAGGATTC TGCGCATTAAAATTTGCAG 074A1F10 AUBES5333 3122 216 18 19.2 CCAGACAGTTCTATGGGCTACC TGATTTGGGGTGGTAGCTTG 108A2D02 AUBES1619 3375 260 18 20.6 CACCGGAGACAATGTACTGG GCGAGATACTGCCGTTTGAT 020A1A11 AUBES4260 1253 374 18 25.2 GCTAAAACTGCGCTCACACC AGCGGCACATAGATCAGTCC 049A1D06 AUBES4182 168 381 18 25.7 ACCGACTCATGACCTCACAG CCGAAGAAATCGGTGGTAAC 082A2E10 AUBES2900 632 474 18 27.6 GGACTGGGTGTGTTGAAGGA CCCCCAGTCTTCCTGATACC 030B1B01 AUBES2696 893 666 18 30.3 TGGTTTGCATCTTGTGGTATG GAAAGTGCTTTCACATATCATGC 019A1H02 AUBES3785 18 306 18 30.4 AACAGCTGTGAACTCTCTCTGTGT AGAGCACGAGCGTGAGCA 072A1C06 AUBES3361 222 688 18 30.9 TGATCCGGGGTTGTGTATATC ACCCTTTCCATGCAAGTGAC 104B2F03 AUBES2826 564 345 18 31.7 TGTGCAGGTCACGCTCTATC TACTGCTCAGAGCGGAGTGA 052B2F12 AUBES3303 564 345 18 32.7 TGTGCAGGTCACGCTCTATC CTGCTCAGAGCGGAGTGAAT 052B2F12 AUBES3391 200 654 18 32.8 TGTTTAAAGCCGGATGTCCT CCTGTCCTGATGACAAACTGG 088A2D01 AUBES4051 133 289 18 32.9 TTGTGTGCAATTCAGGAAGG TGGATATTTGGGACAAAAAGA 087A1G07 AUBES5374 3352 196 18 33.4 AATGCAATATGCTTGACAACCT TGCATATATCCACCTTTGCTT 022B2D10 AUBES2608 222 688 18 33.4 AAGGTGACTGGATCTTCCACA AGGTTTTGCACTGTGCTTTG 047B1C06 AUBES4912 309 311 18 33.4 GGGGTTGTACTTTGTTTTGACC CCCTTGTTTCTCTTGATTGGA 012B1G05 AUBES4682 2844 147 18 33.5 AGCCGGCTAGAATGTTGCT TTGCATTCAGCTTTCCACAG 051B2B05 AUBES2932 63 189 18 33.5 CAGTGGATTTTGTGGCAAGA TGTCATGCTTTGTGGGTCTG 010A1C06 AUBES2812 218 252 18 33.5 CGTGGGGGCACTATACAAAT GCTAGACGAGCTCCGAGAGA 054A2F10 AUBES1539 632 474 18 33.5 CTCAAGACACGGTGACCAAA GAGGCTCACTCCTCCATCTG 030B1B01 AUBES3310 673 318 18 33.5 TGGGTGATTGAGTGGAGTGT TTCCGAAGAGCTAAAAGGTTGT 100B1A07 AUBES5242 2398 151 18 33.5 ACATGCCAGGTTGGTTTGTC TGGGAAGTTTTCCTGCATTC 043A1G05 120 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5271 2536 222 18 33.5 CAATGTCGTGAAGACCATGC CACAGTTATGGCGTGAATACAGA 080B2E10 AUBES3415 406 298 18 33.6 ACGTGGGCCTCTGTAATCAG GTCTTGCATGCTCAGCTGTC 075A1A08 AUBES3414 406 298 18 33.6 TTGACCCAGGACCTCAGTG CTGCTTAAGAGCGAGGAAGC 104B1E10 AUBES5389 3412 198 18 33.6 GTGCAGTGCGTGTTCAGTTT CAGCGTCTAACAGCACACAAG 087B2B05 AUBES3689 603 443 18 33.6 ACCTTCTACCACAGGGAGCA CAATGCGTATGAGGAATGGA 058A2E08 AUBES3263 603 443 18 33.6 ACCTTCTACCACAGGGAGCA ATGCGTATGAGGAATGGAATG 058A2E08 AUBES2501 274 612 18 33.6 GCCATGCGTTCACATGTATT TGGTTCATGGCCTGTGTATG 048A1C01 AUBES1772 singleton 18 33.6 GCATTTTCTAAATGTGGCGTAA CAACCCTGGTGGGGTTAATA 033A1H10 AUBES1768 1050 359 18 33.7 TCTCAGTTGAGCTGCTGCAT TATCCCACCCCTTCACAAGT 034A1D04 AUBES3208 274 612 18 33.9 TCATGTCAACAAGCGGTCTG TCAGACGCTGCATTTTTCTG 077A2D04 AUBES3161 200 654 18 33.9 CCTTCATGGCTGAACAGGAC GCAAAACATGTCCCACCATT 112B1A02 AUBES1980 641 477 18 34.2 TCGAGTAAATGATGTTAGCTGAGG TGGCTATAGTCAGGGGTAAGAGA 022A2G07 AUBES2549 2481 296 18 34.4 AATCCCGCATCTAACCGTAA TTTACACATTTCTGATGGAAGGAA 049B2D01 AUBES3088 222 688 18 34.9 ACGGAGTTCGCATGTTCTCT TGCTCACGATGGCAAGTTAG 073A1D03 AUBES4285 1662 320 18 37.0 GGTCTTTTCTAGATCGGTGTGG TGCTACGTGGTTTGACCTTG 064A1H11 AUBES5018 1181 325 18 39.5 TTCGGCATATCTTTTCACCA GCAAAGTTGTAAACTGAACAAACC 087A2A04 AUBES4615 2115 225 18 39.6 TGCAATTTCAGGACAAACCA ACATTCATGGACACATATCATGG 011A2F02 AUBES4856 1841 245 18 39.9 CGTGTTAGGCTTTCTGAATGG TGGATCTCATGACAGTTGGAG 018B1C04 AUBES2686 639 604 18 40.1 TCAGTGTTTATTTGTTCCAGAAAATC CAACATCTGAAGGGTTTTCCA 009A1H01 AUBES2341 639 604 18 41.3 TGCTGCTTCGAGATTGTAAAG GGTGGTGATCCCATACACAA 047A1B07 AUBES3991 2126 328 18 43.3 GTTGAACGTTGCTGAAATGG TGCAGTTTTCTCCCCAAATG 106A1C05 AUBES2102 singleton 18 44.2 GCCGTGATGCTTTAAGCTGT GCGTGTGCTCGTGTGTTTTA 041B1A12 AUBES2856 1179 622 18 44.9 CACTGGTATAGAAAAATAGGCAGGT CCAAGTGTTGCTCAACCTCA 048A2C07 AUBES5136 1649 409 18 45.9 GGTACTGGGTTCACTGGCTTT TTACGCAGCCAATACTTACCC 107B1C02 AUBES5334 3178 178 18 69.2 CCTTTCCTGCACTCCAGACT TGCAGGCTGAAAGAAACACA 042B1A05 AUBES4738 2323 211 18 73.6 TTCCCCCATTTGTCCTTTCT AGCACACGGCAGGAAAATAC 083B2A06 AUBES4224 528 386 19 19.0 CTTTTTCCCAAATGCCACTG TTCATGCCATGTTTCCTGTG 108A1A12 AUBES4754 2635 215 19 21.1 TAAACGGTTCGCCAATGACT CTGGGAGTGAGGTTCGAAAG 006A2C10 AUBES5338 3202 266 19 22.6 GCCGACACGAGAATTAATGA ACCCACCCGAGTTTGATTCT 080B1F04 121 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES1090 2044 216 19 22.6 ACAGACGCATGACAGCTTCC GTGATGAGTCAATGCAACTCAGG 018B2C07 AUBES2101 1859 271 19 26.3 AGGGGTTGCGAAATCTGAC GGAAAGAAGTGCGTCAAAAG 041B1A07 AUBES3979 1788 348 19 27.4 AGCCTCTGACTTTTCACAAACA AAATGGCTCTCCTCCAACAG 001A2F12 AUBES1971 1299 298 19 27.6 CCACCATGTGCCCAGTCTAT CCAGTCCGACATAGTGAGGA 032B1D02 AUBES2909 948 519 19 31.1 TGCTGTCAGATTTCCCGTTA GACCAGGAGAAGTGGGCTTA 061B2H11 AUBES4241 1053 294 19 31.6 CCAAGCCCAAGCATAATGAA GCCATGTGGAACCTGAGAGT 087B1B11 AUBES1892 181 695 19 33.5 GCATCACCATTCGTGGTCTA TGCCATCAAGCGTTAGCATA 031A1D03 AUBES2348 1376 272 19 34.1 GCAGTGGGTTTTTAGGGTTTT TCTCCAGTGTCGGTGCATTA 047A1G08 AUBES2612 181 695 19 34.5 GTGTGAGATGTTGTTTCGTTGAG GGGACAGCAGTCACACACAC 025A2C11 AUBES3516 173 558 19 35.7 TCAGGGATTGTAACAGCCAGT TTATGGAACGCCCACTAAGC 083B1F11 AUBES2245 1727 455 19 39.2 GCCCAAGTGTGTGTGTGAGA GGCCATTACATTCCGACACT 042B1C11 AUBES2509 singleton 19 39.7 CAACTTTTTCGAATCGTTGTG CGAGGTGTGAAGCAGAATCA 048A2C09 AUBES3755 141 320 19 40.4 ACCTGGCAACATGTGAGCAT ATCACCAAAACGCAAAAAGC 083A2B11 AUBES5366 3307 186 19 40.9 GGATCCACCATACAGAATCCA ACAGCCAGCTCAATGTTGC 048B1E11 AUBES5326 2901 238 19 41.0 CGTTGGTCCGTATTGTGGTA GCTCACCCAACGTCATTAAA 006A1B10 AUBES1699 141 320 19 41.2 GCACGTTTTATTTCGGGATT ACGCAAAAAGCGACATTTCT 034A1H04 AUBES3296 431 316 19 41.3 TTGCTTTTGTGAGCTCTGGA AAGAGGCAACACGGAGTGAT 048B1D09 AUBES1880 1456 309 19 41.7 TTCAGGGTGGTTGTAGAATGC ATGGGAACCTTTGAAGCTGA 027A1F03 AUBES2919 956 352 19 41.7 CCAGTGAACACGTGGCAATA AATGGAGGTTTCAGCCAGTG 038B1E09 AUBES4005 1113 282 19 41.9 TTGTGATGTGGTGTAAGGTTCC TATCATGGGCAATGCACCTA 075B1D12 AUBES4863 1984 419 19 42.4 TATTGAAACACGGTGCAGGA GATTCAGACTCCGGACCAAG 049B2A05 AUBES1946 759 455 19 43.4 TCTGGAAGTGGCAATTAGCTT AACCCTGCTCTTTCCTGTCA 036B2G11 AUBES2354 1984 419 19 43.4 AAACTTTCCAGTCCTTGTTTGTG GAACGCCTTGCCTCTCTTCT 047A2C04 AUBES4034 1360 288 19 43.9 GCAGTTGGGTGTGGTTTCTC ACTGCTGCCAGTGTGAAATG 083A1C05 AUBES2746 114 384 19 44.0 GGGAGATTGTTGAAAGGGAAT AGCACATTACTGCCCACCTC 005B1A11 AUBES5369 3320 213 19 44.3 AACTCGTACCGAGACGAAGC GATGAAGCGTGTTTCACAGC 075A1D06 AUBES4947 659 238 19 44.7 TTCACTGCAGACATCGCTCT ATGATCTGTCAGCCGTCCTC 109A1D02 AUBES3933 1359 271 19 47.6 AAGCCTGCAGCTGTTAGGAG TCGGAAAGGAACAGAAAACG 029A1A11 AUBES2641 26 413 19 48.2 TTGGTGGCTGTTTGTTTGAG GGGATCGTGTCTCTTCTACTGC 009B2E10 122 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2463 1300 463 19 51.1 ACTTTTCACCGTGTGGGAAT GAGCACCATCCACTGACAAA 040B2A02 AUBES5159 1796 340 19 55.0 ATGCATGCGTGTTTGTATGC GATCTCTTCACGAGGCATGAG 052A1B01 AUBES3763 529 240 19 57.5 TGTGAGCGAGTTTGGAAATG TGGTGTCGTGTGCTTCTGAT 031A2H01 AUBES5365 3303 260 19 60.7 TGCACTTGGTTGATATTGGAA GGACGCCACAATTTTCTGTAA 111A2B10 AUBES4942 613 260 19 62.5 CGGATTGCACAATTCATCAG GCGCTTGTAATCCAGACGA 043A1C05 AUBES2163 876 573 19 63.2 TCAGGGGTTCCCAAACTTTA TCAACAATTGGTGTGGAAAA 024B2H04 AUBES4259 1249 228 19 64.0 TCTCAGATTCCCCAGCTCAG TCATTCCCTCCAACTGCTTC 018B1E06 AUBES3770 876 573 19 64.7 TCCACTTTCTCTCCTCCCTTC TGTAAACCAGCCATGTGAGC 085A2G11 AUBES1332 3218 140 19 68.0 GTAAAGTGTCCCTGAGCTGTG CAGATACAAATAGGAGGCACACG 022A1D08 AUBES4585 1948 244 19 70.0 GCAAAGACTCCTGGGTAACG TTGCAACTTGAAGCCTCCTT 104B1A02 AUBES1544 843 333 19 71.9 TTGCCTATAGTTTACGATCACAAT AATGCAAGTGGTACAGCCCTA 019A2C08 AUBES5021 1198 223 19 76.1 CATGTCGGATGAGCTTCCTT GCAACCTAGCCTTGAGATGA 004A1C04 AUBES1284 917 387 19 80.0 TCAGAACCAAACAGATGCAGC CTGAGGTCTTCAACTGCCAG 011A1D09 AUBES4479 1497 282 19 90.9 GCCAGGGAGAACTGAAGACA AACACTGAGCCAAGGAGCAT 038A2G08 AUBES1434 2661 130 20 0.0 CTGGGTCTCCTTCAGCATCT GAACATCACATCAAGTGGAGGA 020A1F12 AUBES4526 874 293 20 1.1 AAATTGATCACTGAACTCATTCG CCATCCACGTCCTTGTAGTG 008A1B10 AUBES4021 1273 267 20 4.1 ATTGGGTGCGTGCTATTTTC GGTAGGTGGATTGGCACTTG 045A2C02 AUBES3801 1029 360 20 5.6 CATCGCTCAAAAGCAGACAC AACAGTATGAAACATGCTCCAATTA 023A2D08 AUBES3009 1502 545 20 5.6 AATAAGTGCTCCCGGAGTGA CAATCAAAAGCTCTGTAACTGAGAG 050A2B09 AUBES3320 1067 514 20 5.6 AGACTGAGATACTAGCGGTGGA TTCAGGATACCATGCCCAGT 047A1A07 AUBES4020 1273 267 20 5.6 TGAACCCATTTTGCACAGAG ACCATTTACATGGGGGAACA 030B1C07 AUBES2798 1067 514 20 5.6 AGACTGAGATACTAGCGGTGGA TTCAGGATACCATGCCCAGT 047A1A07 AUBES2996 1502 545 20 5.6 AAGCCGAGTTAAACCTGCAC TTCGTGAGATTGCGTGTCAT 110A1B06 AUBES5288 2610 194 20 5.6 GGCGGTGAAAAAGTATTTGC ATGCTGGAGCGTAAACAGGT 030B1D01 AUBES3802 1029 360 20 5.9 TCTGCTACTTGCTACTGCTGCT GCGTGACCGGTATGTGAAG 077B2D08 AUBES2415 singleton 20 6.0 GCCATTGTGCATTGTGTTTC TGGATGTTTCACTCCAGCAG 045B1G08 AUBES4379 1563 220 20 6.5 GATGGGGTCCTGAAACACTG CACTCCCCATCACCAAGAAC 024A2H03 AUBES2019 2691 232 20 7.3 CCTGGCTCAAAACACAATCA TGGGGGTTACAATGAACAGC 032A2A05 AUBES3909 1029 360 20 8.9 TCTGCTACTTGCTACTGCTGCT TTCCCTGAGAAGGAAACGAG 038A2A05 123 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2618 193 868 20 11.1 ATGCTTTGACAGATCGCTTG AGATCTGGCAGTCCACAAGAA 073B2H12 AUBES1161 535 331 20 12.5 TCTGATAAGAGCAGGGGTGAC CAGGTCAGCAAGGGGGTTCG 008A1G06 AUBES3741 744 516 20 16.6 TTTCAGCTCTTTGCTTGGGTA GTGATAGTCGGGTGTCCACAT 085B1F07 AUBES3270 744 516 20 17.2 GGGCATCAAATGCAAAAAGT TTAAAACAGTTATTTGACATGAGTCAC 080A2B07 AUBES2694 463 448 20 18.7 TTGCTGGAGCCTATTTGACA AGTCATCCCCATGCTTTGAC 012B1H07 AUBES5382 3395 306 20 19.9 TTGGCACCATTCTGTTTCAC AATGCCTAGGTCACGGTTTC 056B2B02 AUBES2724 283 470 20 21.5 TTAGACTCCGCAGCTTTGTG ATGCAACATTCCCTGGATTC 012B2D01 AUBES1338 580 372 20 29.0 TTTCCAAGCCCGCTGAG GACCACATGACGGCTTCC 025A1E11 AUBES3494 580 372 20 29.8 GGGCACTGTATGTGCATGTT AGTGAAGGGTGTGGGACACT 034A1F05 AUBES2059 959 353 20 33.0 CCGCATAACTGGGATCATTT CGTTTATGTTGGTGCCGAAT 039A2F04 AUBES5010 1134 228 20 34.2 CACCATGGAGTTTCTTGGAGA TTGGAGTGTGTTGCTCAAGG 095B2E10 AUBES1759 1396 186 20 37.2 GCCCTCTGGCAATAACAAGT TGGAAGATGGTACGGGAGAC 027B2B05 AUBES5353 3260 213 20 37.4 CAGATATCCATCTCTGTCCATCA ACAGACAGACCGGCAGATG 022A2F11 AUBES4525 715 409 20 38.3 ACACACCACAGCAAGCAGAG GTTTGCTGGTGTGTTGCACT 085A2E10 AUBES5004 1096 211 20 39.5 CAGTCCATGCACGTTACGAA ACCAGGTGGCAACAAATACA 024A1E01 AUBES2367 singleton 20 39.7 GCCATTAGACAAACCAGATGAA TGTCCTACCCCACCCATATT 047B2H05 AUBES5178 1966 266 20 40.7 GTGTGATTGAGTGCCAGGAA GCCCAAAGAGAAATGGCATA 095B2A08 AUBES4019 1262 485 20 41.8 GGTGTGTGAACAGCACAAGC AAACCTTTTCACTGCCATCG 004B2A11 AUBES4749 2577 233 20 42.1 AGCAAACAGTCCATCGCATA GGTGTCCCCATACCTCTGAC 086B1D10 AUBES2889 481 355 20 43.1 ACAGTTTGTTCATGCGTCCA GAAACCAAAGCGCAACAGAT 033B1B12 AUBES1271 1898 282 20 43.8 TGCCCTGTGGAGGTTGTTGG GTTTCGCCTTTGACTGCGTC 034A1D08 AUBES4787 2902 189 20 45.0 AATGCTGGGATGATTGGATG GCATTCACAAACGCCTAACA 054A2D09 AUBES5320 2772 259 20 45.5 CTCAACCCCACTGAACACCT GCCAGAATGAAAGGAGGAAC 081B2D02 AUBES1277 1167 208 20 47.0 ACGGAGACTCCATGCTGAGC GAATTTACGGGAGGCCGCTG 014A1E03 AUBES2990 481 355 20 47.4 TTGCGGTTGTGAGTCAGTCT TCTCTCGTCCTCATCCACCT 100A2A04 AUBES4746 2548 169 20 47.5 AGAAACCGTTGAAGCTGGAG TCAACTGTCACCTGGTCCTG 111A2C05 AUBES3746 481 355 20 47.6 TTGCGGTTGTGAGTCAGTCT TCTCTCGTCCTCATCCACCT 100A2A04 AUBES1750 481 355 20 47.6 TCACCATATGACGGAATGGA CTGCAGCCAGCTCTTTCTTT 032A1F10 AUBES3560 752 337 20 47.6 TAACGCACAGCTAGGCACAC GCTGTGGGCTCGTAATTAAA 002B2D09 124 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2701 643 629 20 48.2 TGTTGGGTAAGGAGCCAGTT TTGAAAACGCTGTTCCTGTG 023A1E04 AUBES4776 2827 316 20 49.3 TTGGCTCACAAAGCCAAAAT GTGCCTACGCACCTTTGGT 052A2F03 AUBES2928 1284 313 20 50.4 TCATTTGTGAACAACCATACTCC CACACATTCAACCCCTCCTC 054B2A06 AUBES3535 1142 587 20 50.5 CTTGAAGTCAGACAGTACTCATGG CGGCCATGTTTCTGAAGATT 038A1B06 AUBES4660 263 260 20 50.9 CCTGAAGATCATGGGGAGAC CGAGCGCTTTCTAACAGTCA 026A1B09 AUBES3390 281 1157 20 50.9 CTGCCCCCATCATTAGACAT GACAATTGAAATCATATGGTGGA 070A2G04 AUBES3839 2257 316 20 50.9 CGGTTCTGTGGCTTCATTTT CAACGAACGGCTCCATATCT 035B2D06 AUBES4661 263 260 20 51.0 TGAATCTGTCCTTTGCACCA TTCAGACCCAGAAGATGAGGA 095A1D09 AUBES4662 385 468 20 51.0 TCTTCTTCCAGAGGTCTTCCAG TGAGAAGAATCTGCTGCATCC 052A2B02 AUBES1351 singleton 20 51.1 ACTGATCTCGTCCACGGTG GCATTCCACATGCTCACCTC 028B2A03 AUBES3228 281 1157 20 51.1 CCATTGTCCCTACATGAAAGC ATGTGCTCTGAACAGACATGC 039A2G11 AUBES3227 281 1157 20 51.1 AGGGGATTACACCTCCAAGC TTGTTGGCAAGACCATACACA 058B2A05 AUBES3905 942 213 20 51.2 CATCTTGTCCGGTTTTGAAGA ACATCGGGAAGTGACAGTCC 040A2B04 AUBES1591 957 457 20 53.5 TGCAGTTGAGCTCTCGGTAA GCCAAAATGCCGGACTATCT 029A1C01 AUBES3313 714 343 20 54.0 CCTTTACTGCATGGTGTTGC CCTCCAAGCATGACCTTCAC 103B1G09 AUBES3133 130 511 20 54.4 GAATGCTTTTGACTAGGAGCTTG TGGGCTTCTCATTGTGACTTT 041B2C02 AUBES2716 315 426 20 54.6 TGGTCATCTGTGGTGTCTGC TTTTGCAGGTCCTCCTCATC 010B2D02 AUBES4497 2406 164 20 54.9 TTGGTTTATCAGCGGGACAT CGCTCCTACTGTGGTGATTG 013B1F04 AUBES3539 276 739 20 55.0 GCAGTCAGCATAAAGTGCCATA CCAAACTTCAGCAGGTTTTGT 074B1D03 AUBES2933 85 374 20 55.1 TGGCATGCAGATGCTTCTAT TTGCATGCCTCTTGTTTACAT 040B1D02 AUBES2822 482 301 20 55.4 CAAAGTCCAGGTCACTGTCAT GGAACAGGCGAAAGATGTGT 036A1G04 AUBES1710 857 208 20 55.4 TGCCTCAAGATGGAGGAATA AAAGTGCTGGAGTCCTGAAAA 034B1A04 AUBES3382 130 511 20 55.4 ATGTGGACACCTGACCATGA GTAGTAGCAGGGCAGTTGCTG 112B1E07 AUBES3621 228 279 20 55.4 AGCATGTCACACCCTGCATA GATGGCTACCGGATTCAACA 078A1B06 AUBES2127 1559 242 20 55.4 ATCGTGAACAGGGGACAATC CACGTCTGCTCTCTAGCAACA 019A2E09 AUBES4311 1943 225 20 55.4 GCTGGTCCAGGATCTGTGAT AGCACGTTCCTCTCCTTCAG 047B1G05 AUBES5294 2637 188 20 55.4 CTGTGGACGGGAATTTGTTT CAATGCAAATTAATGCCTTGC 021B1E01 AUBES5316 2743 161 20 55.4 TCCCTCACAGCAAAAAGACA CAAGTGTGCAATGCTTTCCT 030B2D10 AUBES3520 355 463 20 55.4 AAGCATGAGAAAGGCTTAGGG TGCATTTCTTCCTCCATGTT 090A1D10 125 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4695 2950 144 20 55.4 TCGCTCTGTCTTTTGGGAAT TTTCAAACCGACCGACAATA 002B1E03 AUBES4236 873 222 20 55.5 AATCGCATGCAATCATAAAGTG ACACTCCCTGAATGGGACAC 060A2B01 AUBES2596 306 589 20 55.6 TTTCACCAGCCACTGTTCTG AATTTCCTGTCACTCTTCTCTCTC 054A2D08 AUBES4237 873 222 20 55.6 CACCTGTTTGGTTTGACTGG TTCAGACTTCCTGCTTCCAA 006B2D09 AUBES5250 2428 169 20 55.6 GGACACATTTGTGCAATGCT CAGGGAAATGTGGCACTCTTA 036A2C04 AUBES5253 2445 140 20 55.6 AACTGCGCTACAGGACTCGT TCGCGAGGAGACAGAAGTG 086B1F04 AUBES4064 459 250 20 55.6 AGAATTTCTCCGGCCTTCAC CTCACGTGGATGAAGTGTGC 065A2D12 AUBES3464 1490 387 20 56.0 GCGCCTAATGTTGATATTCGT CAGTAGGCGAAATGCATGTAA 005B2H01 AUBES3463 1490 387 20 56.0 CGTAGATTGCTGTTGCGACT GGTCGCGTGCACACTATTT 112A1C10 AUBES4045 1512 242 20 56.0 TGCCTACTCTTCCTCCATGTG AGGTGTGCGGTGTGGATAAT 039A2G10 AUBES5030 1226 210 20 56.3 TCCATACAGCTGGCATGTTC CGGATGTAAACAGCAGTGGA 075B1D11 AUBES2778 747 704 20 56.5 TGTGAGAGCGTAAGGCTGAA TGAAAACTTTGCCTCATGGA 075A2A11 AUBES3079 306 589 20 57.0 CATCATGTTTGAAGGCAGGA CATGCAAAAATGTGCAAAGA 021B1B09 AUBES4283 1824 222 20 57.6 TTGTGTTCATGCAGCATAAGATT TGACAAACACAGATGCCACA 109A1A12 AUBES2467 singleton 20 58.1 GCAGATTCAAAACCCCAAAA CAAGCTGTGCTGAAAGCAAC 040B2D12 AUBES2147 2009 249 20 58.5 CAGCAAGGATATGGGGACAA GGGAATGACACCAAAGTGTG 022B1C12 AUBES2882 329 605 20 59.6 GATGGAGGAAATGGTTGGTG TGGTGTCACTGTTCTGTCAGG 073B1D05 AUBES1245 singleton 20 60.9 ACGAGCGAAGAGCGCGTTAG GCCATTGTCACCGCAAGATG AUBES4711 3175 122 20 61.8 GCGCCACATGATGAGAATTA ATTACGGGCCTTGCTTATCA 105B1B03 AUBES4857 1873 382 20 63.8 TCTCCCGCTATATGGTAGGC GCATCAGCCCTGAGAACACT 109B2H08 AUBES4536 2020 174 20 64.7 TGCTTTGGATAAAAGTGTCTGC CTTCCATGCTGGACAGAGGT 024A1G01 AUBES3234 130 511 20 68.3 GCGAGGAGCAGCTCATAAAA CAAGGTCTCTCCTCAGTGGTG 066A1F04 AUBES5047 1313 330 20 83.5 AACGTGCACTTTTGGTGTATTT GGTCACGAGTAGCAGGGTTG 045A2H11 AUBES2564 209 590 21 0.0 CCTGATTCAGAAGTGCTGACC AGAGACGATGGTGCCACTTT 035B1G08 AUBES3001 242 496 21 0.8 CAGGACCTAAACAGCCCTGA AGCACAGCCTTGGTTTGAAG 053B1H12 AUBES2876 242 496 21 1.4 CATCAAGAATCCGCGACATA TGGCTTTAAGACCTTTTGCAC 057B1D06 AUBES5013 1151 441 21 1.7 TCTTGCATGTGTTTGGGTTT GCAGGACAGTGCAAAACAAC 090B2E04 AUBES3336 209 590 21 2.4 GGTTCCAGTTGTTCCACGTT ACATGTTTGTGAAGTGCTGGA 075B2G07 AUBES3087 183 825 21 5.1 TTAATGGGACGGAGTGTGGT GCCAAAACTGTCAGCCTTTC 109A1D11 126 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2607 183 825 21 6.6 AATGCCAAGGGTGTCAGAAT TCGATTCCTCTCTGCTCACAT 044A2D06 AUBES3360 183 825 21 6.9 GCAGACGTATTGCGTCATTT GGCAGTAGATTGGCAGGAAA 072A1H03 AUBES3147 183 825 21 7.4 CAAATCCAGAGAGGGGACAA CCTTTCGTCTGAGGGTCACT 058A1F12 AUBES1569 43 556 21 7.5 TGCATGATTGCCTCATACG CCTCTTGAAAATGAGCAAACG 023A1C10 AUBES2874 212 519 21 8.3 ATCACAATTTGGCCCTTGTC AGCGATTCTGCTGAAACTGG 045A1H12 AUBES1629 1214 512 21 8.8 CACGCCTACAAAACTCCGTA CAGAGCACTTGCATCAGGAG 031A2A05 AUBES4803 3036 252 21 8.9 CAAAGAGCAAACCCCAGTGT ACGCACATGTAGCTCCCATC 050A1D01 AUBES2808 174 352 21 9.5 TGAAGGGGTTTTGTGGTTTC CCCGCTCTTTTACCATAGCA 054B2H05 AUBES2603 455 727 21 9.6 ACCCACTGCTGGGTTAAAGG CAATGCATGTGCCGTTTTAT 013A2F02 AUBES3169 59 1071 21 9.6 AACAGGTTAAATGCTGCTTATGA TCGAATAAGACATGGCAGCTAA 062B2H04 AUBES3948 212 519 21 9.9 TTGACCCCCGTTCTTTTAAC CCATTCGCTCAGATAGGAAAA 038B2A03 AUBES1973 462 357 21 10.3 CCAGTCTGGCTATTTTCCTCTG CTCCTTGTGACTGTGCAGGA 020A2B10 AUBES5307 2700 313 21 10.4 GAAGAAAAATCCAAATGGGAAA CATTTTGTGGCAAAAGTTTCA 074A2H04 AUBES3757 354 335 21 10.5 AGAAGCCATTAGACAGAGAAGCA ACCTGTTTAACGGTGGAGGA 004B2E03 AUBES2936 98 301 21 10.6 TGTCCCTGATGTGAGAACCA GTTTTGCCATGTCGCTGTC 018B2F09 AUBES5221 2284 147 21 10.6 GCATCATGCAGAGATTCCAC AGGCCAGTTATTCATCAGGTT 048B2G12 AUBES2511 1692 173 21 10.6 TGTTGTCTTGTTGAAAGGGAGA TGCGCACTTTTCTTACCATT 048A2D06 AUBES3218 182 768 21 10.6 TTCCCTAATGTGCCTGACATC ACCCAACCCTGAAAGTGTGA 096A2E04 AUBES1879 singleton 21 10.6 TGAAGGTTGACAGCATCAGG GGTGCATTAACCGTTTCTCTG 026B1F12 AUBES2735 1 321 21 10.7 TGCATTCATGCAAAAAGCAC CCATGGTTGGTTCTTGGTTC 027A1E06 AUBES1502 1214 512 21 10.8 TTTAAGGGGTTAAAGTGAAACAA GGCAGAAACGACAGAAAGGA 013A1A08 AUBES3084 455 727 21 11.3 CTGGATGATGGTTGGCTGTA AAGTGCGACCCAACTGTTTC 062A1B09 AUBES3927 1243 293 21 12.0 TTGTCCCACTTCTCCCACAC TGGAGCTTCAGGAGAAGGAG 002A1G12 AUBES3142 182 768 21 12.2 GTTTGGGTAGCTCCATGGTC CCGGCCTAGATAAGATGTGC 047B2B10 AUBES3352 182 768 21 13.1 GGGCTTTGATTGAATGCTGT CAGGTGTGAAAATGCCTGAA 062A2F02 AUBES1700 418 272 21 13.8 ATCGCTGGAAAATGAGATGC CGACTCTTTGCTGAGTTGGA 033B2B02 AUBES2619 39 751 21 14.6 AATGCCCATTTGTCTTCACC GCTTGTAAACCAAGCCATCC 006A1A05 AUBES1168 singleton 21 14.9 TTTCTTTTGAGAGTCCAGTGTGC CAGAGCCAAAGTTCCCTCTG 008A2F08 AUBES3295 418 272 21 15.2 CGGGATCAATTCAACACCTT TTACTCTTTGGGCTGTGTGC 093A1G05 127 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5086 1488 201 21 15.4 TAACAGCAGCTTTGACAGCA CCAACAAAAGTCGACCAAAGT 028A1H09 AUBES2212 122 362 21 16.6 AGGGGGTAAGTAAGCCCTCA CAATCCTCGGGAGCAATAAA 031B1G06 AUBES2099 1007 879 21 17.1 CGGCTTCGAGGAAAGTTTTT CCACGCTTAAAATCGATACTGC 041A2F10 AUBES3643 742 284 21 18.9 CCTTGAGCTCAGGAAAAGCA TTGGGATAGATTGACTGCTTGA 031B2A02 AUBES3642 742 284 21 18.9 CATTGATGAAGTCAAGATAGCTAGAGA AAGGGACAATTGATGCTGGA 054B1F05 AUBES3534 1118 626 21 19.1 CAAGAGGCAGGGCTGAACTA TTCAATGTTTCGGGTGTGTG 095A1A12 AUBES2755 239 565 21 20.1 TTAGGCCATAAGCATGCAGT GGCTTGCACCTTCCAATAAG 035A2F09 AUBES3523 402 570 21 21.7 GAGAGAAATAAGGCAGTCAAAAGC TGATTGTTTACACACTGTTGGTG 054B2H06 AUBES5069 1404 233 21 22.8 AAAGGCATTAGAGCCTGGAT GCCGAAAAGCGACTAGTTTG 106A1D07 AUBES5012 1149 291 21 23.1 TCTGCAAAAATTGCTTGTGA GTGAGTGGAGCGAAGGTCTC 061A1A05 AUBES3232 9 2006 21 24.9 TGACTAGGCTAAAGGCTCAACA TGACTGTTTCGCATCCTCAG 006A2G05 AUBES1418 2706 171 21 31.0 TGATGGGGAAGCTGAGAGTT TGTCAACATGTTAAGCACACTAGC 032A2G10 AUBES4997 1060 215 21 34.9 GGTTGAGGGCTGTTTCCTG ACCGCTGAGAGGAGTGAGAG 035A2C09 AUBES4095 1001 281 21 36.8 CACACGCTGCACACAATAGAT TCATTGCTTTTGTTCGATGC 006B2C06 AUBES4096 1001 281 21 41.1 TGATTGATGGGCATATTGTAGG CATTAAATGCACGCAGTTGG 046B2E04 AUBES5283 2586 276 21 43.6 CAACTTGGAACACGCAATACA CCAGTAATCGGAAGCGTTTG 052A1C04 AUBES3242 41 502 21 45.2 GTTCTCCCCGGCGATATTAG AGTTGCACAACAGGACATGC 105A2C08 AUBES5405 3496 220 21 45.6 ACCAGAGAACAGCCGGTAGA GGACCTCGGGATCGTTATTC 048B2A10 AUBES4419 2530 428 21 50.2 GCTTGTTGTCACAGCGTTCA CGAACACACAACTCCCACAT 092B1F04 AUBES1945 103 445 21 50.2 TATGGTTTGGGGTTTTGTGG CTACCGGCTGACACCTTCAT 036B2G10 AUBES1624 103 445 21 59.1 AGCACTAAGCACAGGTGCAA CCTCTTTTAGGTCGGCTGAA 023A2H12 AUBES3972 1695 304 21 61.9 ACCTCGGATAAAGCAAATGG TCATGTTTTCCCTCAGAGGAC 105B2E05 AUBES3971 1695 304 21 62.9 GACCCATGATGCACTGAAGA CAGCTGTGTAGCACGTTTACC 090A1H02 AUBES3556 586 413 21 63.9 GTGGGTCATTTAGGCAAAACA TGCTAAATACGGGCTCTGCT 007A2D02 AUBES4405 2092 335 21 65.3 TCCTTCTCCGCCGTATTATC TGATTGCAGCTCGTTAGTGG 094B1F03 AUBES4404 2092 335 21 69.8 CATGAGCGTTAGTGCTTTGC CTGCCAACCATTACGAGGTT 046B2G06 AUBES1299 singleton 21 72.0 AGCAGTTTAGACATGGCTGC TCCAAAACCATCCGCGACAG 021B2E09 AUBES1298 singleton 21 72.3 AATTCACTCACACAGCTCACAC ACTCCAGCAAAACATGCAGC 021A2F07 AUBES1405 singleton 21 72.4 TCACACAGCTGCTGATCAAA CAAAACATGCAGCCTGAGAG 021A2F07 128 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4026 1341 274 22 0.0 TTTACTGGCCTCCAACTGGT GATGGAACCCCATCAGACTC 052A2E09 AUBES4025 1341 274 22 0.1 CGGTCCTCAACCACTACCAG GTATGGTGTCCTGGCGAAAT 108A2F04 AUBES4775 2825 205 22 3.9 GTTGCGCTGATACTCGTTTG TCGGTGGTTAAAAAGAGAACG 110A1C06 AUBES5239 2390 215 22 6.2 ACCAAACAACGATGCTGAAT TCGGGCAAATATGAAATTGA 051B2A09 AUBES5368 3316 134 22 12.9 GTGTGTCAAAGAAGGTCACGA CGAAGCCGGTCTGAAAGTTA 052B2A07 AUBES4459 785 220 22 14.7 TTTGTATTCCGTCTCCTCTGC AAGTGCTCCGAGTGCTGTGT 035A1H01 AUBES4094 975 259 22 20.3 GCCGCTTACTTCAGAGAGCA CAGGTCCAGCATTCAATCAG 003B1D03 AUBES1448 973 583 22 26.0 ATGCAAGCAAATCGAAATGG GGTCAGTGTCCCCTCAGAGT 027B2C11 AUBES4779 2856 233 22 27.1 CAGCCTGTTTGCCAGTAGAA CACGACCCAAAAACAGGAAC 109A2E09 AUBES1941 973 583 22 28.7 TCTCTCTCTCTCTCTCTCTCTCTCTC TCCTGTCATTTGCATGCTTC 036B2D11 AUBES4723 3317 183 22 30.3 TGCTTAGGCAACTGCTTTCA ACCATGCACACACACAGAGG 025B1C02 AUBES3925 1166 345 22 40.4 CCTCTCATGCTCCCATGAAT CGTGATCGGCTCTATGACAA 104B1E08 AUBES3167 166 890 22 59.8 AACGTTTCAATGGGTGCTGT GGTTGTGTGACAAAAACGACA 108A2E02 AUBES2635 166 890 22 59.8 TAACGTTTCAATGGGTGCTG GGTTGTGTGACAAAAACGACAC 009B2F10 AUBES2232 166 890 22 60.6 TGTATCGTAGGACGCCATTT TGCATTCACAAGGCGATAAA 042A1H02 AUBES3378 166 890 22 60.9 AACGTTTCAATGGGTGCTGT GGTTGTGTGACAAAAACGACA 072A1C05 AUBES3163 252 835 22 61.9 GCACTACCCTCCAGTGCTTT AGTACCTTGGGCAGCATCAG 053B1E09 AUBES1863 singleton 22 62.1 AGTTGTTGTCAGGGGCTTCA GGGTGCAGTCTATGCAGGTC 021B2H11 AUBES1593 1912 262 22 62.9 AATGGCATTTGGCTGAAGAG CATTGCCTCGTCCAGAGAATA 029B2E02 AUBES4854 1761 266 22 63.2 CACACACACACACCCAAACA CCTGCAGGTCTCTGTCACTC 048B2A07 AUBES3814 1496 249 22 63.8 TATTCAGCTCCCGAATCACC TCAGTCTTCTGGCCTTCACA 048A2C03 AUBES2593 135 619 22 63.8 AGGCTAAAATGGCTGGGTTT CCTGGAAATGAGCTTCATCC 045B2H12 AUBES3553 1017 309 22 63.8 TGCCTCTATTTGCCTGTTTC TGTATAAAGTGCCTTGAGAAGCTG 092B2D03 AUBES4930 558 286 22 64.5 ATGGCGTAACACTCTGAACTCAT CTTGCATCCTTTGCAGTCAA 053A1F01 AUBES1323 1451 216 22 64.7 ATGACCCTCTGCGGAACCAG CCAGTGTTTTCAGCCATGTGAG 023B2F01 AUBES1968 39 751 22 64.8 GCCTGGTTTTACGCTGACAT CGCATTGAGCAACTGTGTCT 030A1F10 AUBES4670 2760 162 22 64.9 TCCAGCTAGCGATGTTAGGTT GGGTGACGTGTTTTTGTTTG 077B1G08 AUBES3156 39 751 22 65.1 GCTCGATAAAAGGTTGACAAAG CCCACTATGAAAACACACAATTT 029A2D07 AUBES3815 1496 249 22 65.6 AGTTGTGGGTGGTGGTGAGT TTGTGTCCCACCATTGTTTG 094B2A03 129 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3059 107 496 22 66.0 TGGCAGATCTCAGGTAGCAA AGCCACAGGTTTAGTCTCAAAT 060B1F12 AUBES4488 1578 218 22 66.1 CTTTGGGAGAGCTCACTTGC CTGAATGCATGGTGGTTGAG 112A2G02 AUBES2830 635 462 22 66.1 TGTGTATGCCTAGACAACATGG TGCACTGTGAACAGGAGCTT 046B2A10 AUBES3729 475 313 22 66.1 GGAACCCCAAATTTCTCTCC AGAGGGGAGCTGGTGTAGTG 065B1F08 AUBES4844 1642 316 22 66.1 GACTCATTGTGGCCCAACTC AGTAATGCAGGCCAATCAGC 059B1C06 AUBES4845 1642 316 22 66.1 GAGAGACCGAGAGACCGAGA AGTTCTGCATGCACACATTTC 106B1E07 AUBES2565 107 496 22 66.2 GCCAAAGAGCCACTTCAACT GCTCCTTGTGCTCTCCAGAC 054A2C12 AUBES3406 358 269 22 66.2 GCTCTGGATAATGAGGGTGTG GGTCGAGCAGAACAACGTCT 038A2C08 AUBES2817 395 413 22 66.2 TGACTTTGATCATCGCATGG CTCACAATTTTATTTTGCACCA 034A2F02 AUBES1996 2848 203 22 66.2 CATCACAGAACCTCCAACCA TTTGGGGGAAATTCACAAAG 027A2B06 AUBES3187 135 619 22 66.2 TACCTTGTTGGCACAGCAGA TTTGAATCGACTGTTGCTCAG 051B2C10 AUBES2591 404 868 22 66.2 GACGCAGCTAAGTGCCAGAT TGTGCTGGTTAGCCAAGGAT 075B1A10 AUBES2369 1045 232 22 66.2 TACGGAAGGCTTTGGGTAGA CGGATTTGGGATAACTCTCG 044A1A08 AUBES2737 19 431 22 66.3 AACAAACATCCGACCTCTGC TGGTACACTGAACTGATGGATGA 063A1D02 AUBES3198 252 835 22 66.3 GATGTGGTTCCAACCTCGTTA GGACTTGGCAGATGTGCTTT 108B1D11 AUBES3719 453 468 22 66.3 ATGATCAAATCGGACCAACC CTCAACTAATGCCTCTGTGTGTG 039A1C09 AUBES3297 474 318 22 66.3 TTTGATGTTTGTCCCGATCC CAATTTCCCTTGTGGAATCA 039B2B04 AUBES3456 1017 309 22 66.3 GGATTCCTCTGCATTTCTGC GTGAAGTATCAACCTAATCATTGACA 014A1A01 AUBES4470 1242 189 22 66.3 CGCTTCAGCGGACATAAAGT GGGACTTCCACTGCATTTTC 096B1B07 AUBES1227 1548 286 22 66.3 AGGGCACAGGCAAGCCACAC GCTCCCAATAGTAAGCCTAACTGG 028B1F11 AUBES4558 1740 193 22 66.3 TGATCAACGCACACAAGTCC CACACAACAAGCTTCTGGACA 086B1D04 AUBES5393 3429 174 22 66.3 ATGCCACAGTGATGGAATGA ATCGCACCGAGAGAAGAGAA 112B2A02 AUBES3192 29 847 22 66.3 GTACATCACCTGAGGGCACA GGGAGCCAAGTGCATAAGAC 008A1G04 AUBES3038 66 298 22 66.3 GGAGCCTTCAACTGAAGAAAAC TGTTGAGCTGTTTCAAGAAATG 062B1G08 AUBES3292 389 362 22 66.3 ACAGGCAGCTGGAAATAAGC GTTGTCATGTCTTGGCCAGT 076A2F08 AUBES1717 820 605 22 66.3 CTACCGGAGTCACATTTCCA GTTGCGCAAACGAAATACAG 033B2D12 AUBES3813 1496 249 22 66.3 AGTTGTGGGTGGTGGTGAGT TTGTGTCCCACCATTGTTTG 003B2D06 AUBES1702 887 333 22 66.3 TCCCGGGATAGTCTCCAGAT CAACACAATGCCATCATTCC 033B2G04 AUBES2645 120 445 22 66.3 CGAGACATTTTGTCCCAACA TGCAAAGTGACACTGAATGCT 023B2H08 130 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3405 358 269 22 66.6 CACCCTAATGGCATCGATTT TGTGCAGACAGGTGGGAATA 084B2E02 AUBES5031 1230 320 22 66.6 CAGCTACTGGAGGGTTGCAC GGCTTGGTGTACTTCAGTGGA 007B2H11 AUBES3916 1111 259 22 67.4 TCATCATGTGCTTGCTTCCT CACACCGGTTCTTTCAGGAT 079B2H08 AUBES3518 240 551 22 67.6 GCAAATCCTGAGGAGGATCA AAAGGAGGGCTGTGAGTGAC 072B2E06 AUBES3807 1148 203 22 67.8 GCAAATGGGTTTTTATCAGCA CCCAACCACAACAAGATCAA 107A2B10 AUBES2271 358 269 22 68.3 CCCACAAACAGGACAAGTAGAA CATTCAGATGCTTTGCAGATG 043B1B07 AUBES5209 2206 179 22 68.9 CAAATTGCTGCTCATGCTGT AATGTCCGAGACATCTGTGG 009A1E04 AUBES2237 453 468 22 69.5 CACATGGAGCCAATAAACACA CTTCCCTGAAACTGCTTGCT 042A2E04 AUBES2888 453 468 22 69.7 GGGGAGAATTTGGAGAATCTG CAGGTAACGGGATGGCAATA 048B1G07 AUBES2389 166 890 22 69.9 ATGGTCAGATTACCCCAAGC TTCAACGATCAGCGTTTAGG 044B2B11 AUBES2047 487 272 22 69.9 TGCCCTCTCTTTGCTCTCTC GCCTCTGCCATTTTTATGGA 039A1C08 AUBES3519 240 551 22 70.8 GAGTGAATGTCCTCAGGGACT TCAACAGGGGTCATTTAGCC 102B2E03 AUBES3109 166 890 22 71.2 TCACCTGCATCCAATTCAGA TGGCACCCTTGGTAAATCA 011B1E05 AUBES4636 293 222 23 0.0 GCAGTGGTTGCTGGTCTTTT TGTCCATCTTGGTGATCTGC 087A1E07 AUBES2875 237 389 23 0.6 CAGGATATAAACGGCCCTGA TTTCCGAAGAGGGTATTCACA 078B1B07 AUBES4200 449 468 23 0.6 TCAGGCATTAGGATCGCTTT ACTGCTGTTCTGGAGCCAAG 087B2A04 AUBES4266 1370 348 23 0.6 AGCTGCCACTTTCACTTTCTG CAGCTATCGGATCCCCATTA 050A2C07 AUBES4804 3052 194 23 0.7 GCGGACATGGACACTGATG GTGCGCGTGTATACGTGTGT 022B1A09 AUBES5091 1517 546 23 3.7 GAACTCACGCTGCTCCACTT CGTCCGTTCACGTAAAGCTC 073A1E04 AUBES3033 421 266 23 5.0 TTGGTGCAGTGTGTTTCTTTG CGAAAATCAGTGTCGCATTC 058A1F07 AUBES3847 2697 203 23 5.6 TACCTTGCATGCTGGCTTTC ACTCCGCCTTCTGAGGATTT 073A1E09 AUBES4735 2288 252 23 7.3 CCCGAAACTGAAGCTGATTG TGTCTTTTCCTGCTGCTCAA 035A2D03 AUBES4392 1857 360 23 7.4 TGTGGAACTGTGCCTTTTCA CTGTGTTCCAGCATACTGAAGG 079B1F02 AUBES2255 382 555 23 9.8 CGGAGTGATTTTCCGTCATT GTACACTTCCCGCACACACC 042B2B08 AUBES3046 382 555 23 14.1 TTCAGGTGTTAGTGCATGACTGT TAGGAGCCAGCATCCTTTTG 074A2E03 AUBES2736 12 457 23 17.3 CCTCGCTTTCTCTCGTTCTG TTGACGGGGATTTTAGTTGC 004B2G06 AUBES3102 74 900 23 18.6 GGAAGTAACATGGACCCAGAGA AGGAATCAAATCCCCAAACC 110A1H06 AUBES4900 90 274 23 18.9 TAGCGTTCGTTTATGCGATG TGATCGAGACATAAGGGGTGA 051B1G04 AUBES2025 371 409 23 20.0 GTCCTCTCGGGTCTCCTCTT CAGACCCACACACACAGACC 038A1D10 131 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3188 211 954 23 22.0 CAACAAATCTCGTGGGAACA GTGTGAAAGCGCTCATCTGT 005B2B08 AUBES1606 301 379 23 22.6 GGTTAAACAGCTAGGTGCACTG ACAGCCAGATGATTTCCAGTT 031A2G03 AUBES5129 1622 215 23 23.5 ACATGCGGATGTGCAAGTAA GGCATCTCTGCTGGATACCT 042A1C01 AUBES3159 74 900 23 23.8 GTTGGAGAAAGACCGCACAT ATGTGGGAGAGCCCACTAAA 087B1A01 AUBES4137 1040 364 23 23.8 ATACACATCCTGCCCTCGAT CACTGTTCCTGCCTTCACAT 068B2C07 AUBES5394 3443 145 23 23.8 ACGGAGACGAGGGAGAAAAT ATCTTGTTTCCCGGTGAATG 084A2G11 AUBES3217 211 954 23 24.6 CAGATCCTTCCCAGTCTCCA CCAAAATGGGGAAATCACAA 011B2C08 AUBES1361 650 433 23 24.6 TATTCTGGCTGGAGGCTACG GTTTGAGTCGGCAGCACTAC 024A2D06 AUBES2251 727 200 23 25.1 CATATGCACAGACACACATGC TGTTGAAATACTGGCCCACA 042B2A07 AUBES2484 982 284 23 25.1 GATGTTTTCGATTCCAACAGG CATAAAGGCCGTATGGGAAA 046A2D03 AUBES3691 809 443 23 25.1 GGTGGCGTAACCAGATTTGA TGACCTGATTTCTTCGGTGTT 019A2G04 AUBES4412 2434 272 23 25.1 CTGGGATAGGCTCCAGGTTC GGGGATGCAGTTAAACTCAGG 077A2F09 AUBES2937 105 276 23 25.1 AGAAAAAGTCGCGTCTGTGC TCTTCCCTGTGTTCCTGCTC 054A2H08 AUBES2625 74 900 23 25.2 CCAAAGCCGGTACCATAAAA ACAGCTGTGACGTTGGACAC 008A1A03 AUBES4132 982 284 23 25.2 CACTGGGGTGGTATCCTCAT CCACCTCGGCAGTACTTTTC 062B1C09 AUBES4010 1169 260 23 25.2 GCAGGAGCCTGTATTCTTCC TTGCCTCCAGATGCTAAACA 032A1D12 AUBES4113 1419 318 23 25.2 CATCCTGGCGAAGCTCTTTA CCATGCGAGGCCAGTAACTA 110B1G04 AUBES5084 1470 196 23 25.2 CAGCTCTGACAACAGCATGAG TCAGGTGTATTTGCCCTTGAT 002B2F12 AUBES1330 2434 272 23 25.2 AGCCTATCCCAGGGGACTTG CTTACCCATGTATCATTGGGACC 033A2G04 AUBES5354 3268 205 23 25.2 CTGAAGCCAGTTCTCCTGTG CAGCATGTGAAGTTGTGTTGG 073A1D07 AUBES5359 3283 399 23 25.2 CACTGTCAAGCTGCAAAAGC TGATTCAGTCTGCTGCTTGG 078A1G06 AUBES4136 1040 364 23 25.6 TCAGGGGAAATTATGGCAAG TGGCAGTGCTTTATCCACAC 068B2A07 AUBES5065 1382 193 23 25.6 CGGAAATCCATTGTTTTCTTTC GGAGCTCCAGATTGAATGATG 073B1D02 AUBES1801 1227 232 23 25.7 GCAACTGGATTATGCAGCAA GAAACAAAGGCAACCTGAGC 033B2C01 AUBES3657 211 954 23 26.4 CTCCACAGGAAAGCCTTTGT ATGCCGTGTAACTCCCACA 083A1B07 AUBES5009 1130 360 23 26.4 TGCTTGAGTGGGCACTATGA CCGCACCAAACTAACAAACC 104B2C05 AUBES4148 1229 255 23 26.5 GAGAGCATGGCCAACAACAT TTGTCAGCAGACAGGTAGGC 095B2D06 AUBES4147 1229 255 23 26.5 TGTGTATTTACAGCCAAAAGCA GGGAAACATGTTAAAATTCTTGTCT 086A2C08 AUBES5301 2671 294 23 26.5 TAGCGGTTAAAGGGGCAAC TGTCATTTTTCGCAATTTTCA 027A1A10 132 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5285 2601 264 23 27.3 GCTTTAGGGGTTTCCTCCAA GGGGACTCAAGGCTCAAAGT 048B2F01 AUBES5302 2672 259 23 27.8 TGGAAGTGGCTGAATTTTACA CCTGGACTGGTATTGGGTTC 074A2D07 AUBES4313 1947 198 23 29.7 GGTGCTCGCTGTTAACCTCT TCTCCGAGCCGCTGTTATAG 077A1A06 AUBES1506 200 654 23 30.8 AAGCGATGATGACAGCTCAC AACCCCATGCACATATCACAC 013A1E05 AUBES5386 3401 269 23 32.3 TGTTCCTGCTGTCTGAGCTTT CCTCAGTCCTTCCTTCTTGCT 005A2C05 AUBES5211 2215 299 23 34.7 TCGACAGTTCGAGTGTCCAG TTGGCTTGTGAGATGCTGTT 048B1G11 AUBES2962 1038 276 23 35.5 GATTATTTCGAGCGCGTAGC GCTCAGGGGATCATAAAGGA 074A2C01 AUBES5238 2389 154 23 37.4 TTGTGATGAGTTGCATGACG GCAGGTTTTCCCCTTTACCT 108A2H12 AUBES4683 2855 179 23 38.4 CAGGATAGCATCAGGACGTTG GGTACAAGCGCGCTAAATTC 060B2D09 AUBES4327 2114 198 23 43.3 TGTTCCTGCCACTGTGTTTC ATCTGACCGTCTGTTTGCAG 046B2C05 AUBES1900 2114 198 23 45.8 GCACAGGCAAATAAATGCAA AAGCGCTGACACTGGAGACT 036A2A08 AUBES1411 2855 179 23 46.2 GTGATGAGGAAAGGGACAGC ACGCTGTTAGGGGGTTGAT 024B1H05 AUBES4116 1465 484 23 47.8 GGGCACATGTGGTAAAGAGG AGGGAGAGGGATGGAGAGAG 011B2F08 AUBES4115 1465 484 23 48.0 GGGAGGGTTAAAGAGGGTGT GATCCTGCATGAGTGTCGTC 081B2H09 AUBES1877 singleton 23 49.4 CGGCCGTTTATAGCTTCTGT TGAAGGCAGGATAAGCGGTA 026A2E12 AUBES4032 1351 245 23 53.4 GACTCCTGAGCTGGTCTTGG ACGGTGGAAGCAACTGAGAG 105B2C03 AUBES4580 1904 206 23 53.6 GCTGAAACGCTTTAGGTCCA CGATTTGAGACGCATCTGTT 086B1G08 AUBES4676 2803 201 23 53.6 TCCACATGAGCGCATTTAAG AAGCAACAGAAAGCCATTAAGAG 092B1A11 AUBES1328 1351 245 23 53.7 ACACAGAACGAGACAGGTGAG AGGTACTCCGACCTCCACAG 022A1H01 AUBES4031 1351 245 23 60.7 CTGCGGCATAAAACAAAATG GTGGTATAATCCGCAAGAACG 062A1G09 AUBES5291 2619 208 23 71.9 GACGCCTATTTTCAGGCATT ACCGTCGTAATCGTCAGGAG 066A2E08 AUBES4290 1697 269 23 84.5 TTCTTCACAGCAGGTCGTTG ATGGCTATTTGGAGCGTGTC 039B2D09 AUBES4289 1697 269 23 86.2 TCTTCACGTAGGGATGACACC CTGGAGCCATATTCGCAGTT 031A1G02 AUBES3062 790 651 24 4.8 GCAGTGTCTTGACAAATGGACT TTGCACAAATTCTCACTTGGA 004A2F01 AUBES2490 295 359 24 5.2 AGTGCGCAGTCTGTGCTG AAGACACACAGGAGGCCTTG 046B1C02 AUBES1978 3272 140 24 7.2 TGCAACATTTCCCCAAGTTT GGGGACCTTATGGCACTGTA 022A1H11 AUBES2563 246 494 24 11.2 TGGCAAACTTTTGTTTGAACC CATCACGTGAACCAGCTCAT 022B1F03 AUBES2583 890 863 24 11.2 ACCAGCTTAAGACAGCAGCA AAGACCTTCTACACTACAGTGATCTTT 032A1H04 AUBES3281 188 296 24 12.5 ACGCTTCCTCCAAGTCATGT CTTGGTGCGAGGGTTGTAAT 112B2E07 133 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3931 1338 490 24 12.9 TTCGTGTACGAGTGCCAAGA CACTGCTCGGGGATGTAACT 074A2C10 AUBES4630 1997 240 24 13.7 CTACCAGGTTGGGCAGACTC TGTTTCAGTTCGCTGCACTT 061B2A05 AUBES3711 398 761 24 14.1 GAAATCTTTGTATGCCTGGGTA AGAGCAGGCCTCTCCTCTG 023A2F11 AUBES2513 838 260 24 14.4 TCCCTGATTAATGCCATTCG GAGAATGCTTGCACATTCCA 048A2F03 AUBES3902 915 218 24 14.4 ATGGCATGAGAACATCAACG GGAATCACGGGAAGACGTTA 036A1A07 AUBES2788 589 303 24 14.4 ACTCCAGGTGAGGGGATGT TCATTCATCGCCAGATGGTA 055B1A06 AUBES3335 246 494 24 14.4 CAGAGCCTCCGAATGTTACC GCTTTCTGTGAGGATGCATTT 083A2D09 AUBES2658 372 438 24 14.4 AACAGGAGCAATTCTGCAATC GCACAGCCTTCACTATGCTG 057A2H10 AUBES2848 444 296 24 14.4 GGAAGGTTCTGTGCCGAGTA GCCCCATCCTGATTTCTTCT 030B2E03 AUBES3305 589 303 24 14.4 CACTCCAGGTGAGGGGATGT TCATTCATCGCCAGATGGTA 055B1A06 AUBES3073 890 863 24 14.4 GCAGCGTGACACGGTTTAT CCCCTGCTACTTCACCATTC 011B2H02 AUBES4742 2436 223 24 14.6 CATTGCTCCAAAACCACAGA GGGCATCAATCACTTCAACA 048A2B03 AUBES4995 1048 272 24 14.9 CCCTGATGGAAGGATCTGTT TGTGAGCATTGTGACCCATT 034B1G06 AUBES3481 295 359 24 14.9 AGACACCGTCGTATCGCATT GACTCTCCACAGGCATCACC 111A2G04 AUBES3375 50 1049 24 15.0 CGTGTGCTTTATTTGGCAGA GGACCGAGTACCTGTTCCAG 106A1B04 AUBES3206 246 494 24 15.0 CAACAGACTGTGCCGGAGT ACGTGGTCGATAAGGCATTC 035B1H08 AUBES4917 415 282 24 15.0 CACTACGAGGGCAGGGACT GACGAACAGCATTGTGGGTA 014B1B07 AUBES5008 1129 401 24 15.0 GGAAAATCCTCCGCTTATTCA CAAATCCGACCATGACACTG 107B2A03 AUBES3041 823 411 24 15.0 CAGGGTCAAGTGGATGGAAA TCACATGGCTGAATTTGGTG 062B2C02 AUBES1551 878 338 24 15.1 GCTCAAAGGACAGCAGAACC AGGGTTGGAAGCAGAGTTGA 025B1A12 AUBES2720 823 411 24 15.1 TGTGAAAAGGATAACTGCATTG CATAATGAGGAACTGGTCTGGA 012A1A04 AUBES3693 838 260 24 15.5 TGCTTTGGTACTTCCCTTCAA AGCCTGCAAGCAGAATTATTTA 010A1E04 AUBES4826 3157 127 24 15.7 ACACAGAGCAGGGACGTTTC TTCTCTACCGCCCAGCTTAC 082A2B06 AUBES3910 1043 166 24 16.1 CCCTCTCTGAAACCCAGCTA GAGCACAGCCTGAGAAAAGG 030A1F04 AUBES5179 1985 282 24 16.4 CGGGACTAAAGGATTGGAAA AGAGGGAGCTGCAAGAACAC 006B1D10 AUBES5063 1375 240 24 18.0 GTCAAGCCAGGCCACATTAC GCAAACTGTCAGCCAGTCAT 073A2A06 AUBES4865 2094 374 24 18.8 ATCCTTCTGCAGCTCGACTC GAGTACCGGCTTCTGAGTGC 052A1A02 AUBES2681 398 761 24 19.7 GTTGACGGTGATCGAAGACA GGGGTGTAGACAGCCCTTTT 006A1D04 AUBES4360 695 565 24 21.0 ACCAGGAGCTTGTTGACTGG TTCGTTCAGGAATCCAAACA 080A2D12 134 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4864 2094 374 24 22.3 TCAGCGTCAAGAGACCAACA CATAGTGGCGCAATCAACAC 013B2F04 AUBES3503 695 565 24 22.7 ACCAGGAGCTTGTTGACTGG TTCGTTCAGGAATCCAAACA 110B2C06 AUBES3502 695 565 24 24.2 CTTGACCCTGATTGGGTTACA CCCCTCTTTATGGGAAGCTC 054B2B10 AUBES4728 3543 101 24 29.1 GCCTTGGTCCTTTCATCTCA CACCTCTTCTGCAACCACAG 063B1E09 AUBES4616 2117 222 24 30.8 GAAATTGCCGGGAAAGTGTA TGTCTATTATGGCATTTTCTCCAA 028B2A09 AUBES2732 550 524 24 31.5 GAACGCTACTGTGGAGCTCAT AGTGACCAGGTGAAGGATGG 022B2F01 AUBES3926 1225 399 24 36.6 GCTTCACTAAGACGCCCTCA TCTTTCCAAGCCTCATTTCG 047A2H06 AUBES1334 1480 215 24 40.3 CCCAAGTGCCTGAGGCGAGC GTTCACGAGGACGTGGGTCG 002B2G03 AUBES1956 1107 568 24 42.2 CAACCGATTGCAGGGTACA CCCAGTCCAAAGGCATACAC 021B2D08 AUBES2068 2882 223 24 49.0 TGTGCGTTAACTCAAATCCTTG GCAGACCTGTCGTCTTAGCC 039B1B10 AUBES5003 1095 255 24 50.6 CGAGCGAGGGATGTACTTGT CAGCACGTCCCGAAGTTTAT 006A2C03 AUBES4807 3078 145 24 66.3 CCCATTTTGCTGTAGCAGGT AGTTCCAGTTTGTGAAAATGTGT 071B1D07 AUBES4381 1626 259 24 68.2 GAAGTCAAGCGCACTCTTCAG GAAGATGTATCATATGGATAGCTAGGA 001B1C10 AUBES3944 146 274 24 69.1 CCAGATCACTCAAAGGTCCAG TGAATGATGGGAATGTCTGA 031B2F11 AUBES4674 2779 222 25 0.0 CATCATATATTGGTTTGTTTAAATTGG TGGTGCTAATACGATGATACAAGG 109A1E11 AUBES2383 singleton 25 3.5 GGCGTGAAGGAACAAGAGAA AAAGGAGGACGCGTCTACAA 044B1A05 AUBES4141 1123 568 25 12.1 AGATCAACCGCTTCCAATCA TCCATGAGTTCTTCCGCTAAG 012A1B08 AUBES1179 singleton 25 16.8 ACTCCATTGGTTTGAGGCAC ACGTGCATTAGTGGCCCCTG 011A2C01 AUBES1857 1123 568 25 17.4 TCACTCTCCTCTCTGGCACA CGTTAGGCGTCGGTCATAA 036A1G11 AUBES2418 singleton 25 18.0 AAAAAGGTATTAATTTTCACTTCAACA AAGTCACTTTCTCATGTAAATTTTTG 045B2B05 AUBES1858 1123 568 25 18.9 GCCTCCATGTCCTCCATTTA CCTGAACGGAGCAGAGGTTA 036A1G11 AUBES4142 1123 568 25 19.8 GAATTCTGCCCATTTCTGGA ACTGGATGTGCCGGATTACT 086B2A08 AUBES2530 277 702 25 21.5 CAGAAACTCATGTGGCCTGA GCCTTGCATAAGCTCCTGTC 049A1E03 AUBES5295 2641 274 25 27.8 GCTGCTTGTGATGAGCTCTG ACGTTGAGCGAAATCGTCTT 103B2B12 AUBES4180 81 585 25 38.6 GCTTCTGTATGCATGGACTTCT AGGGATTGAGATTACACGGAGA 108A1A05 AUBES5188 2062 382 25 42.4 CACTCGACAAAACCGTGTGT GTTAGCCTGCGTTTCTTTGG 095A2C10 AUBES5184 2015 211 25 43.5 CTGTCCTGTGCACTCGTAGG GATGGGCCATGACTCCTTAC 104B2D05 AUBES3489 469 311 25 44.9 TGCTCTGTGTTACGTGTTTTCA GACTTCAGGCTCTGGAATGG 038A2E02 AUBES4926 507 279 25 45.0 CCTGCTGCCTTAACCAATGT ACAGGCGGAGAACGTACAAC 102B1E10 135 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4632 2029 260 25 46.0 GGTTTTAACGCACACCTTCC AAGCTGGCTGCACGGTATTA 004A1E03 AUBES4179 81 585 25 47.3 GCAGCTCTGAGAGTCCTTCC TGACAACGGCAAAATAGACAA 103B2A11 AUBES4295 1768 198 25 47.7 CTTGCGTGCACAGATACAGC CACTGAGCTGCCTTCCAAAT 112B1C08 AUBES4087 1886 474 25 53.9 CTGGCCCTTTAATTCAGCAC CTGTTGTGTGGAACGGTGAC 084A2H02 AUBES4990 1000 193 25 55.2 GGACCGTTTCTCTTGAGTTGA GAATCGGCTTTACCATTCCA 004A2G06 AUBES3101 177 881 25 56.0 GGGGGAAATTTCATTTGACTT GCTTCAGTGCTGTGCAGTTT 046A1B09 AUBES2450 572 271 25 57.0 GGGTTTTCTTTCCATGCTCTC ATCATCTTTGCCTGGCTTGT 040B1C08 AUBES3042 572 271 25 57.2 ATTTGGGGGTATTGATGCAC CTTTGTAGAATGCACAATGAAGG 062B2G04 AUBES5024 1204 284 25 57.8 CATGCAAGACCTGTGGTGAG GGCACGTGATGCATTTTTCT 020A1B02 AUBES3920 1147 260 25 58.1 TCACGTTCCCCTACATCTCA GATGGTGGACAACAACAACAA 112B2G09 AUBES5166 1846 281 25 58.9 ACCTCCATCCCAAAACACCT GCTTTATATGGCCATGTGATCTT 006A2C11 AUBES3243 70 413 25 59.0 CAGAAAGCTGCTGCATCTCA TTTGTCATGACCACTAAGGTGTG 110A2B02 AUBES3047 884 490 25 59.0 GAGAAGATGGAGCCTGTTGC GATTATCCTGAGCGCCACTG 074A2G10 AUBES3505 16 545 25 59.2 CAGACATGAATGTGGACTGACA CACATGGATGAAGGAAGCTG 109A2D11 AUBES3985 1897 326 25 59.2 CTTCCCCAGTCCAAAGACAC GGTGGAATCGACACAAGGAG 096A1H08 AUBES5118 1550 266 25 59.9 TGGACTTTGGAAATGAAGAGAAA CACCGATTTTGAAGGTTTGAG 096A2E12 AUBES4451 1507 189 25 59.9 GACCCCAATCCACCTACTGA TTCCTACCTGCCATTCCTCA 029A1D02 AUBES2475 144 430 25 59.9 CACAGTCTTGGAGGGGAAAA CCGTTAAGAGCCCTTTCAGA 046A1A12 AUBES3099 392 1013 25 59.9 ACCTCGATGCACAAGGAAAT TCACCAGGAGGGGATAGAAA 004B1C05 AUBES3687 483 257 25 59.9 GCTGCCTGCCCAGTAAAGAT GGTCAGAGGGTGTGACCTGT 013A2B01 AUBES4029 1348 286 25 59.9 CACGTAGGCGTAGATCGTCA TACGTGGACCATAGCCTTCC 104B2G08 AUBES1873 177 881 25 60.0 CTGCCCTCTGATACCTGCAT TTGCCTGCTGAACATGATTG 025B1H07 AUBES2869 144 430 25 60.0 GGAACTCTCTGTTCCCACTCA TTAAGCCTTGGTGGTTCGTT 060B1H06 AUBES2656 357 320 25 60.0 GCCCTCTGGGAGTTTAAGGA TGCATGCCAGGATAACAAGA 001B2A05 AUBES2553 411 719 25 60.0 GGTTAGTGCTGAACGAAATGC CGTCCAGAGAGAGGAATGGA 012B1G02 AUBES2847 434 347 25 60.0 TGGCAGATTTGTTTCCATCA GGCATGGTCATATGGTGTTG 012B2C10 AUBES2770 505 384 25 60.0 CCAGAATTCACACACCATGC CCAGCTTGAGCTGCTAGGAC 023A2C10 AUBES4284 1851 404 25 60.0 ATTTCAGCACCTTGGAGAGG GGGATGTGCCACTAGAGACC 030A1E12 AUBES1798 1992 167 25 60.0 AGAGGCTTCAGTGTGACTTGG TGGATGCAAGTGTGTGTGTG 034B2H02 136 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4028 1348 286 25 60.0 TCTCTGCATCACTGCGAAAT TTGACTTGGCCTCCAAATTC 007B1B04 AUBES3157 392 1013 25 60.0 TGAGGACTTCTGCATGGTTTC AGGAAAACAGGGTGCTGTGA 010A1F03 AUBES4646 554 298 25 60.0 AGGGTGTGTTGGCTTTCTTG CAAACAACTGTTGCCTCGAC 065A2F06 AUBES4450 1507 189 25 60.1 TGCCTTAAGGGAGCTCACAA ACATGCAAACTCCGCACAC 004A1G05 AUBES2624 177 881 25 60.4 TTGGGGTTTTTACCAAATGAAG TGTCTGTCCAGCTATCTATCTACCTA 003A2D09 AUBES5254 2455 210 25 60.6 AGCCACCCTCCTGTATAGCC GCGAGAGTGAGAACGAGTGA 108A1C07 AUBES2677 678 526 25 60.6 CCCAAATTCAGAGAGCCTGT TGTGGTCTTTCTGTAAGGTGTCC 003A2H10 AUBES3547 451 311 25 60.6 CGGACATGGTACTGGTGATG GCAGTGAGAGGGAGACCATT 108A2B12 AUBES4548 2830 338 25 60.7 GAGAGGACGTCGGTGTAGGA AACACCCCCTCATTTTCCAC 011A1H01 AUBES3419 451 311 25 60.7 GATGGCTTGGTTTCAGTGCT CTGTGGGACAAGTGAGCAAG 040A1G09 AUBES3032 599 494 25 61.2 TGGATCCAGAGCCTATTCCA CTTGCCCATAAGGGTGAAGA 056A2B01 AUBES1749 1851 404 25 61.5 ACCTTGGAGAGGGAACGTCT CTGTCTCGAAGCTGTGCTTG 030A1E12 AUBES3901 842 232 25 61.6 CAGATTGTGCCTGCTGTGTC TGAGCTGAAGATATTCACAGGA 058B2H01 AUBES2904 678 526 25 62.2 CACTGCTTATTGAAATCCATGC TGGGAGTTAAGGCAACTGAGA 058A1B09 AUBES1570 599 494 25 62.8 ATGGCACACAAAAGCATGAC GCAGACTCAAAACACAGCAAA 023A1E06 AUBES5248 2425 183 25 63.6 AAGCCACAGACTGGAAAGCA TGAGCTCAGGATGGGAGAAT 034A2B01 AUBES2621 392 1013 25 64.1 TGTGGGTTTGAGGGGATTAC TGAGATAGGCTCCAGGTTCC 053B1B08 AUBES3467 1598 433 25 64.9 TACACAGTTGGGTGTAAGTAAGGTA GCCCAAAGTTCAGGGTTTCT 085A2D10 AUBES4951 687 355 25 65.2 CGTCCATCTTTCCAGTGTTC CGTCAGTCACGAAGATCGAC 024B2F06 AUBES1960 1494 242 25 65.6 TGAATTAGCCACGACAGACAA AGCCACCAGAAGGCTAAAGG 023A1D10 AUBES4619 2132 411 25 66.2 TCTGGTGCAGTAAGCTGTGG CCTCGCTCATTCAGAAATCC 105A1A03 AUBES4921 432 325 25 66.9 ACGTGGCTAGCAACTCGTTT TGCCGTTTATTTGTGTACGG 042A2C03 AUBES4679 2811 200 25 69.7 AAATCACAGGCTACCGCAAC CTCAGCATCCACCTGCAAT 011B1D09 AUBES4474 1298 274 25 70.4 TCCAGTGGAAAAGAGCCTGT AGAAGCCAGAAGTGGGTTGA 075A2E11 AUBES4791 2929 262 25 70.4 TGCCATGTTTTCTCTGCTTG TGTATGCAAACTGCATTCGTC 102B1C07 AUBES4736 2295 238 25 70.8 CCGGCGATTAATTTGAACAT ACCGGTGTTTAAAAGCCTCA 030A1G02 AUBES4793 2942 201 25 71.0 TCCCACCACCATAGAGCAAT CATCAGCACGAACGCATTA 023B2E03 AUBES4530 1797 316 25 71.7 AAGAAGCGTTTGCAAAAAGG GGGCACTGCAGGAAGATAGA 052B2E04 AUBES5230 2354 230 25 74.1 GGGTGTGGAACCAAATCAGT ACACACGCAAACGAAACATT 102B1H02 137 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4452 1596 279 25 75.2 CGCTTAATAGGACGGCTGTT ATGAAAGGCACAGACGCATT 032B2D06 AUBES2491 782 304 25 77.1 CCGATCATCCTGATCTCCAG GCGTTTAACCCTCAAACGAG 046B1F03 AUBES2074 2953 242 25 84.5 CCTCTGCTCGGATAAGACGA GCATCAAATGACAGCTCGAA 039B2B06 AUBES4291 1704 303 26 0.0 CGACGGACGAACTCCTATGT TCCAGTCGCTTACACTGAGC 063A2C02 AUBES5414 3541 110 26 1.6 AACCACTGTCCTGAGGGATG GATATCCTCGGGGAATAGGG 108B1G01 AUBES4753 2634 145 26 10.1 GGATTTTGCCTTGCCCTTAT ATTAGTGATGCCTGCGAAGC 051A1F05 AUBES1808 singleton 26 12.0 CTGACGTCCTGCTCTGTTCA TATGTGGAGACGGGGTCAAT 034A1D12 AUBES4603 1982 223 26 15.3 CAATCGCAGGTGGTCAGACT TCGACTCATCTGTGAGCTGAA 049B2C03 AUBES1964 1536 440 26 18.3 TCCTGTTTTCCCTTCACAGC GCTCCCCATCTCTCTGTTTG 027B1G12 AUBES4984 979 348 26 18.4 GCCTGGCCTTCTTAATTTCG GGGAACTTTCCCTCACTGCT 054A1B12 AUBES4836 1536 440 26 18.4 ACCTGGGTATGAACCACGAC ATATTTGACGGCTGCTTGAG 112A1C11 AUBES4837 1536 440 26 19.2 CACATGCAAACACACACTGG CCCTGCTCTCAGGAATTCAG 003B1H01 AUBES1891 2357 266 26 19.2 CGCACGACTCGGACAGTAAT GCAGAGAGTCATGTAGGGTGTG 030B1F12 AUBES4618 2122 233 26 25.2 CGTCGCTTCAGTGGATTCTG TTATTTTTCATTTTGTATGTATTCTGC 053B2B05 AUBES5406 3512 157 26 25.7 GAGCTAGCTAGGCAGCATGAG CAGGTTACCCCGCTAGCTAGTAT 014B2D09 AUBES4411 2357 266 26 25.9 CCACCCCGTCATTGATTTAT TCGGCATGTCCACAGTGTAT 074A1D06 AUBES4794 2955 178 26 26.4 TTCGAGAGAAAGGTTTGCTGA CAGGGACCGTAACTGGTTTG 084B1A03 AUBES4805 3059 161 26 26.4 GGTCAGTCCCTCATCGACAC TACCCTCACACCACAAACCA 111B1A08 AUBES4410 2357 266 26 29.5 CGCACGACTCGGACAGTAAT TCTGAGTCACGCCATGAAAG 030B1F12 AUBES4634 255 227 26 31.4 GCACTCTAACTAACCAACCCACA ACCACCTGCACAATGTCATC 001B1D04 AUBES1865 1314 570 26 31.5 TGAAACTGGATCAGAGGCTTT CGTGTGGAGCAATTTGAGTG 022B2C01 AUBES4239 923 210 26 31.7 CAGGGGATTTGTCCCATCTA TTGCCCATAACACAGGGTCT 021A1D08 AUBES1301 singleton 26 31.9 TCCTTCACTGCTGTCTGAGC GCAGTCATCCAGAGTCCCAG AUBES1300 singleton 26 31.9 CTATGTTCAGATGACCCATGAAGG CTATGTCAAGACTATGGCGATGTTG 021B2H07 AUBES1333 399 249 26 32.6 TTGTGAGGGTCCTCATGCTC GGATTGTACGTTCTGCTTGACG 014B1H11 AUBES4877 399 249 26 32.7 GTTTGTGAGGGTCCTCATGC AAAATCCCACACGTCAGGAG 052A1C01 AUBES4230 760 277 26 34.2 TGCATGGAGGAGTGAAGTTG AGGGTGATGAGAAGCAGCAG 075B1G11 AUBES4741 2403 210 26 34.6 TTCCCTGGAGGAATGAAGAG TCACCAACCACCTTCTGTTG 072A1H10 AUBES5052 1334 357 26 35.0 GCCCCAGGGTGATACAGTAA GCACGGACAGCACCATCTA 085A2C02 138 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3034 1232 633 26 35.2 AACACAGCGCCTCAGATAGC TAGCTGGGCATGGTGAAAAT 058A2E03 AUBES4439 1427 238 26 36.3 GAGGAAGCAGGCTGAACTTG CCAGGAGCTCTGTCTCGACT 108B1F03 AUBES2789 658 328 26 40.6 CCAACTGACATGTTTTCAGGA TGTTAGAGGTCAAAGATGGATGA 007A1D10 AUBES4292 1732 250 26 40.8 GCCATGAAAATAAAGCCACAT TGCCATTAATCTCTGTGAAAAAG 023A1E05 AUBES1595 singleton 26 41.6 GGGTGCAATTTTTCACATGG CATGGGCATGATCACAGACT 021A1F09 AUBES4825 3155 220 26 42.2 TGACTTAAATCAACATCATGTGC GTGGCCACCAAATTATCAGG 059B2A08 AUBES1872 658 328 26 43.0 CTGGAATACACCCTGCATGA TCCTGAAAACATGTCAGTTGG 007A1D10 AUBES2372 142 418 26 45.0 CCGGTTATTCCGTGGGTATT GTCGGCGTTCAAAGTTCAAT 044A1C03 AUBES2698 142 418 26 45.7 ACGGAAGCTGATTCGCTTAG GTCGGCGTTCAAAGTTCAAT 019B2G07 AUBES4312 1944 242 26 47.2 GGACTGGAAGCCAGATGTGT ACCCGAGAACACAAACTTGA 029B1D08 AUBES2923 1106 446 26 47.5 ATCACGTTTGCTTGTCCAAA CCACTGTTGCTGTGAGCTTG 008A1F03 AUBES2824 532 272 26 47.8 GAGCAGGTCGTTTGTGTCAA TGCTCTTTCCAAGCCTTTGT 020A2F04 AUBES4520 663 227 26 47.8 CTTCAACCAGGCCACAAATC AGTGTTCCACTTGCATTGAGC 004B1B11 AUBES3091 40 671 26 48.2 ACTCTGAGCCTGAGGGGAAA TGGAAGAAATATGAGGATTCTGAC 106B2C01 AUBES3095 403 966 26 48.4 ACTGCTGCATGTTCTGGATG TGACGCCTTCTGTTTTCTGA 084A1F12 AUBES5413 3538 123 26 48.4 GCGTGGTCTTTGTACTGTGG AACTGTCGTTACTAAAAGGCTCA 043B2H10 AUBES3764 541 386 26 48.6 AGCACTCTCAGTCACGCTTTC GGGATGAACTTCAGGACAGG 043A1F08 AUBES3153 403 966 26 48.7 CCCTGGCCATCTCAAAGTAA CTGCTGTAATGTCGCAAAATG 052B2H05 AUBES2477 1398 526 26 48.7 TCATATGTGCATGGGATTTGTT CCCATCACTCACACTCTCCA 046A1C04 AUBES4194 360 416 26 48.7 GCTGAGAATTAGCCGCACAT ATGACAGCTGGCGTAACACA 110B2F05 AUBES2547 403 966 26 48.8 AGCCAGCAGAACAGCTTGAC GCCTCTCCCTCTCCTCTCTC 049B2A11 AUBES4934 575 470 26 48.8 CCCGTTTGCAAGATGTCTTTA GCCCTAACAATTTGGTCTGC 073A1E05 AUBES4218 1621 487 26 48.8 ACAGGCAGTTTAAAACCATTCC CCTTCCACCTTCCATTTTTG 013B1E02 AUBES1847 40 671 26 48.8 GCTATTAAAGGCTCGGAAGG GCCTTCAGCAGGGAAATCTA 036A2B09 AUBES4333 2201 293 26 49.5 TCCCACACTTTGTGGTTTCC GCTGTGGAACGTCCACTAAAC 095A1G08 AUBES4220 1621 487 26 49.8 GTAAGGCATGCACAAACACG TGCACACAGTCTGTCCAAGT 047B2C09 AUBES4130 972 220 26 50.3 CTTCATTGATTTGGCAGATTGA CCAGTTCCTATGTCTGGACTCTG 002B2A06 AUBES3911 1049 267 26 50.6 CACAACTCAGCAAGCACACT GCTGTGTTGCACAGGCTTAT 068B2H04 AUBES1754 51 233 26 51.0 AGAGAGGCCTTTTCACAGCA TGTAAAGTGCCTTGAGAAGCTG 024A1G05 139 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3593 1093 462 26 51.8 TGACGTGTGGTTGCTTAAAGA GACCGATGTTCCTTCACCAT 090A1E09 AUBES3592 1093 462 26 52.1 CACGCCCTTTCACAGTACAA CTGCCACCCATCCTTCTATC 087A2G05 AUBES2615 403 966 26 55.0 TCATGATGTTCAGTAAGTTCAAAGG CATTTGTTGTGTGAGCAACATT 072A2F09 AUBES2655 333 279 26 57.5 GCTTGCTTGGTTATCATGTGC AACAGGACGCAGCAAAATGT 023A1A09 AUBES5060 1366 362 27 1.6 CACGTTGGCCTTATGACCTT GCTGCGATATTTAAAGGTGTTTCT 073B2C01 AUBES4058 359 309 27 13.7 GCACAACAGTGAGCGATTCC GGTTTCGATACGGAGCGTTA 035B1C05 AUBES1727 singleton 27 20.4 CAAAACAGTCCTGGCTGACA TCGATCCGTGGCCTAAATAC 023A2C12 AUBES4914 353 308 27 25.5 CCATGTTGAATATCCACCAAGTTA ATGCAGTGCTCGCTATGCTA 048B2C04 AUBES4944 638 271 27 29.6 GCATGCTTCTGTCATCTAGCA GGTGTTGGATAACCTTTCTTGC 064B2H05 AUBES2117 9 2006 27 30.4 CGATATTTCTTACGCTCCACA TGCTTTGAGACAGCGTCCAT 041B2H10 AUBES3260 463 448 27 32.1 ACCATCGCACCTAGCAGAAC AGTTGGAGCCAGGTAAGTGC 046A1B03 AUBES4107 1281 250 27 34.4 GCATATGCCTGCACAAAGAG GTGGTGACATGCTGTGAATG 109B1D02 AUBES3571 844 271 27 35.8 GCCTCACTGGGTAACTTGCT CAGTTGACATTTTGCTGACG 033B2G03 AUBES3572 844 271 27 35.8 GCCTCACTGGGTAACTTGCT CAGTTGACATTTTGCTGACG 084B1F08 AUBES1533 2578 183 27 36.6 CAGCAAACAATCTGATGTGGA AAGCCTTCCTGTCTGTCAAAG 023B2F11 AUBES1281 2724 179 27 38.6 TGGAGAGAGTCAACACGCAG ACCATAATCTTGCCACAAGCAG 019A2C04 AUBES4654 758 277 27 39.3 GGGGCTAAACTCGGCTTATT TGAACTCTTATGATGGGAAAACTT 061B1D09 AUBES4334 2227 254 27 39.3 TTCGACATAGAACACCAGAAGG TTTGTTTTCAGGCTTGTTTTG 083B2H09 AUBES1395 1858 299 27 39.7 GTTCTGGGGTCGCCATTAC CTTCCTAATCTCTTGTGGACAGC 018A1D06 AUBES2629 154 1130 27 40.1 GATTAAACTAATGGATGGAGAATCG GCGAAGTCATTCAGCGTTAG 012A1D01 AUBES1714 990 286 27 40.1 CCCACCTCATGTCTGTGAGT TTGCATCCTCTTCCTGCATA 033B2D03 AUBES2412 1791 352 27 40.1 TGAATAGCCCAGGAAGCAAT GCAATGAATCCGAGAAACAAA 045A2F08 AUBES3231 9 2006 27 40.2 CTTGAGCAAGGCCTTTAACC TGGCTGTGATTGATGAGTGC 064B1A10 AUBES3959 728 766 27 40.2 CACTGGTCACAGGGGGATAC CACAGTGCTGGAGATGTTGG 102A1B03 AUBES3462 1196 266 27 40.2 TAGCAGGAAATTAGCGGTCA CACTTGCACAAATGCTTCCT 022B1H11 AUBES5207 2195 293 27 40.2 CCACATCGATTGGTGGTACA CACTCCAGCTATGGCAAAGG 069B2E11 AUBES2124 990 286 27 40.2 TTGACTCAATGGGCTTTCTG AGAGGGTTACACACGGCATC 018B2C02 AUBES3732 1139 266 27 40.3 TCTCAAGCATGTGAGAAATCTG AAGTAATCAATGCGGCCAGT 073A1D08 AUBES4966 832 340 27 40.3 CAAACAGGAGCAATGGAAGG TACTCATGGCCACACAGCAC 109A2A07 140 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES5053 1335 196 27 40.6 TGGCACGCTTGATGCTATAA AAGGACGGGCTGAGATGAAT 098B1E11 AUBES3372 154 1130 27 40.7 GATTAAACTAATGGATGGAGAATCG GCGAAGTCATTCAGCGTTAG 084A1H10 AUBES1773 990 286 27 40.7 TCAGCTTCCCTGCTTGATTT CCCAGTGTCCCAAAAAGAAA 033B2D03 AUBES3918 1010 386 27 40.7 CAGAGCAGAACACAAGGGTTT AGGAGTAGTGGGGCTTTCCT 062B1C04 AUBES1721 463 448 27 41.5 ACCATCGCACCTAGCAAAAC AGTTGGAGCCAGGTAAGTGC 019A2F09 AUBES2693 744 516 27 41.6 AAGGCCTTGAATCATGTTGG TCGATTTGACACCTCACCTG 012A2D07 AUBES1747 1335 196 27 42.0 TTCTGCTCAGAACCCTTTGC GCAACATGACAACTCGGCTA 074B2G08 AUBES3021 1387 648 27 43.0 TGCAAGTGAGAAAACACCACA CGCACATACACAGCGTTTCT 047B1G04 AUBES3737 1387 648 27 43.1 AGCTGCATGGGCTTTCATAA CTTTTGGCTTCCACTCAAGC 077A2E06 AUBES4109 1377 264 27 44.4 ATTGGGGTAGAGGCAGTTGT CCTGGAACCATATTGGCATT 010A2F12 AUBES1878 2270 272 27 45.8 TGAGGATCAGGCAACATCAG GAGTCCCTGCTTGCACTCTT 026A2G10 AUBES4378 1551 201 27 46.4 TTGTGCCTAAGCAACAACTCA CATGGCCCAGTGAAATCTG 072B2G03 AUBES4672 2775 198 27 48.5 ATTGCCGAAGCCTAATTTCC TGTATTTGTCCGCCCTTAAA 006B1G03 AUBES1547 1551 201 27 50.6 CTGGAAGCAATGTGTTGTGC TGGCCCAGTGAAATCTGTTT 008A2G06 AUBES1951 1377 264 27 51.0 CTGCTGAGATATGGAGGAGGA CCACCCCTCCCTTTGTTTAT 020B2D10 AUBES5225 2324 210 27 63.4 CAGGGAACTCCCAGCAGTAA AAAGGTTTGTCCTTCGCTTG 073B2A03 AUBES2273 singleton 27 66.7 CTCGCTGGTGAACTCTAGCA CAAAAGAAGGTTCGATGAGG 043B1D05 AUBES5025 1212 282 27 73.2 CCAGCAGATTCTGGGAGATT TGCCACTGGAAATTATGGAA 053B1G08 AUBES1279 3339 288 27 80.5 CATGCAGCACATGGCCGTGG TCGTGAGCAGAACCCTGGAC 022B1G11 AUBES5000 1078 387 27 86.5 GATCAGCTGGTGGAACGAAC GCGAAGAGGTCCTGAGTTTG 056A1E11 AUBES4623 2165 320 27 93.8 TTTTACCCTGGGAACGAATTT CATTCTGTGCAGCGTTCACT 047A2A01 AUBES4317 1964 228 27 98.2 GAACTCATGATGGAGCAGCA TCAGACCACCCAGTGTTTAGG 021A2D07 AUBES4946 656 286 28 0.0 CGGTGGTTAAAGGTGACGAT AAGGGAGCAGTTTTGTGGATT 071A2A11 AUBES2721 230 357 28 0.7 TGTTCCCTGTCACTGGACTG TTTCCCAAATATTCCCAGTTG 012A1B09 AUBES3430 581 353 28 0.8 GAGGACGCTGATGGAGTCAT CCGGAAATTGGATTCTGGAT 054B2C08 AUBES4355 581 353 28 0.8 CTGCATGAGATTTAAAGGGGTTA GGGGTCATGACGTGAGAAAT 093A2G05 AUBES3288 318 453 28 5.0 GCGTGCATAAATGAACACAA TACGCCTCAGTATGGCACAG 103B1E07 AUBES5205 2184 196 28 5.0 TTCCCTTTTCCAGGTAGTGC CTCTCAATCAAATCTGCTTCCA 078A2G04 AUBES1789 318 453 28 6.1 CGGGGCACATCTTACAATTT CGTGCCATGAAAGATGACAA 034B2C05 141 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES1979 460 690 28 11.5 GCGTAAGTGAGAACAGGAACG TAAACTGCGGAAAACACCTC 022A2D02 AUBES5033 1239 282 28 15.4 CGACGTGTTTCCTGTTTAATGA GGAGGACGTTAGCGTGAAAA 110A2A09 AUBES4747 2550 223 28 19.2 CATGTGGAGCACCCATAGTG ACCACTGTCACACCGTCAGT 007B1F07 AUBES4748 2554 277 28 19.2 CGGAAGGAGTCTCCAGTTACG CAATCGACTTCAGGATGGTG 084B1H05 AUBES3028 21 291 28 28.0 GGGAAATTCCTCAAGAAATCG GTGGTGTGAGGGCTACATGA 053A2D05 AUBES3888 1288 242 28 28.4 ACGCAGACAGAGCTTCCAGT CCAGCCCTTAGCACATGTTT 046A1E05 AUBES5290 2616 238 28 30.6 ACCGATTAATGCCCTTGGAT GGATATGGCTAACATGGCTGA 106A1C11 AUBES1736 1902 516 28 31.0 CCCACCCTATGTCCTTAGCA GGTGAACAATGGCTGGAGTT 030B1C05 AUBES5213 2230 244 28 34.3 GCTCTCTGGGTGTCTTCGAG GGCTTTAGTAAGGAGGTGATGAA 014A2D07 AUBES4890 951 210 28 35.8 GTACCCCAGGTGTGTTTGCT CTCAGGAGTTTTCCTCCACTG 020A2E12 AUBES4891 951 210 28 36.0 ACCCTGCACTGCTCTCTGTC TGCAGGCGGATTGTCTTAG 108A2A11 AUBES3956 582 758 28 36.2 TTGCGATATCCGTAAGTCATGT GCCGTACAAAGCAGCTCATC 075A2E04 AUBES3078 335 744 28 37.3 TTACACGCCATACAGCCTGA GACTCTGAGCCACGGAAGTT 055A1H05 AUBES2895 582 758 28 38.8 AACCGATTGGTCAAGGTTCA TGGGAGTTCATCCAAATGGT 064A1A04 AUBES3563 762 379 28 43.0 CGTTCCGTTATCATCGTGTG CATGCAATGCAGGTTTGAGT 029B1D01 AUBES4726 3467 186 28 46.5 TTGTAGGTGCACGGATGAAG TTGTGCTTTCGCGATAAAAG 032A1H05 AUBES4608 2038 291 28 47.9 CATCGCGTTCTGATCTCTCA CCATCCGGTAATGCAATTTA 042A2D12 AUBES3564 762 379 28 48.0 CTTCACGTCCAGCAGAGACA GCTGGGTATTGGATCTGAGC 005B1B09 AUBES3176 1363 940 28 48.6 TCACAGGGTTGCACTACAGC ATTTTTGACCGGGGGTGTAT 034B1E03 AUBES2651 192 303 28 55.4 CAACCAGAGCAACGAGGTTT TGGGTAAGCGTAGTGAGCTG 054A1C08 AUBES2375 9 2006 28 56.5 GCAGTCCAGAAAAGGACAAGA CCACCCACTCAAGTTGCATA 044A2A07 AUBES4027 1343 326 28 57.7 GCATTGCCGATCAGATTACA CCATGCAAAAATGCTTTCCT 077B2A01 AUBES2578 5 418 28 60.0 TGGTATTCTCACAAGGCAGATG CATACCAATTGCAGGTTCCA 055A1B10 AUBES4061 437 257 28 62.0 CTGGCCAGGATGAATCAATTA CAATGTTCTGAAATGACCCAAA 078B1B08 AUBES3550 581 353 28 62.8 CTGCATGAGATTTAAAGGGGTTA CTGGAACGCTTCATTTCCTC 044A1C12 AUBES4062 437 257 28 63.2 TGGCCAGGATGAATCAATTAC CAATGTTCTGAAATGACCCAAA 086B2F11 AUBES4823 3148 194 28 64.1 TGTATTGCTCCAACCTGGAA TCCACCCATGTTTATGAATGA 049A1A08 AUBES3686 437 257 28 64.6 TGGCCAGGATGAATCAATTAC CAATGTTCTGAAATGACCCAAA 086B2F11 AUBES3068 5 418 28 65.9 TTAAGTGCATGAGCCCACAC TGTCCATCATGATTCCCAAA 104A1A03 142 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES4894 22 291 28 66.0 CTGGATAAGTTGTGTCCCTTGA CACTTTTTCCGAAATCACAGC 008A1F11 AUBES4370 1076 484 28 66.6 ACACGAAGCTCCATGTAGGG GAAAGCGCAACACATTGAGA 094B1B12 AUBES2077 2913 171 28 66.9 AGGAACTGTGGCATGAGAAA GAGAGAAGGCCAGAGGTGAG 039B2E11 AUBES4788 2913 171 28 67.1 CGGCGTCGCTTCATATTCTA GAGAGAAGGCCAGAGGTGAG 039B2E11 AUBES3589 1076 484 28 67.7 TGCTTCCCATACCAGTGTGA ATGCCAATGAAAGGTCCTCA 028B1F04 AUBES3022 781 438 28 69.8 CCTGGAGGTGTGAGGTGAAT CCAATAGTGGAGGGCACTGT 049B2E04 AUBES4255 1202 293 28 70.0 AAGAGATGGGTGCTCAGCTT GCCAGCTCATGAAGTGTTTG 029A1B01 AUBES4655 138 453 28 70.0 AAGCCTCCATGGGCTAGTAGT TGGGAATGAAAGAAATTGCAC 080B1A07 AUBES4275 1589 406 28 70.6 GGTTTGTGCAGGCATAGAGA CCTCACAGGAACACATGCAG 036B1D07 AUBES2546 495 866 28 71.1 TTCATTGCAGGGTGCCTTAT GTGGGTTTCCTCCAGGTTCT 049B2A02 AUBES2684 147 416 28 71.2 CGAAACAGCCAGCTACCCTA CGTACTGTGTGTGCAGTGGA 007B1E07 AUBES3094 155 884 28 71.4 CAGGCGGACCTATTGTTTGT TGGAAAAGGACATGCATCAG 013B2C01 AUBES4657 138 453 28 71.6 CATGCTGGCACAAGAGAGAG TGGGAATGAAAGAAATTGCAC 112B1D03 AUBES3364 276 739 28 71.6 AGGGTATCCCAAAGGTCTCC CTGGGATAGGCTCCAGGTTC 078A2C02 AUBES2613 276 739 28 71.6 ATTTCCCCACAAAGGCAAA TAAAGGAAGCAGGGGGAAAT 004B2B09 AUBES3756 285 288 28 71.7 TGGTGCAAGAGATCGAAGAC GCCCTAACCACTGAGCTACC 064B2A08 AUBES3193 276 739 28 71.7 TTCATTGCAGACAGCTGAAAG TGGGGAATATCACAAAGTTCCT 001A1A10 AUBES4656 138 453 28 71.9 GGATGGCAGAGAAGGAATGA TTTCAGAATTGGGGTTGTACG 109A1E05 AUBES3152 155 884 28 72.5 CAATGTGAGGAAGCCTGGTC GTGTTTTTGGTTGCCCAGA 048A2G04 AUBES1748 155 884 28 73.6 TGACTGTGAGTAGTTTCCCTGCT CACCTGCAACTGCACTATGA 028A2F04 AUBES4901 115 244 28 74.6 GGGGAGGAAACTGGAGTACC TCTGTAGTGGGTTTTCAGCAA 001A2A05 AUBES2870 147 416 28 76.2 CAAGGTAACGTAGCCCTGTTTC TAGGGCTTCTTGGGTGGACT 060A1B08 AUBES4582 1918 249 28 78.4 GGCTCAACTCTCCCACAAGA CTCCATACCGTGTCCTGCTT 105B2A11 AUBES4581 1918 249 28 79.0 TGGTTAGTGGTGGTGAAGCA CAATTGGTGGACAAGATCAGG 014A1D06 AUBES3340 262 795 28 80.0 CATGATTGAGGGGAACACTG GGCTCTCGCTTGATCCTATG 074B1B10 AUBES3179 262 795 28 80.3 CACTAGATGCAGCACTGGTAAG GGATGCCTGTTGCACTTCTA 063B2F02 AUBES3341 262 795 28 80.4 TGGAAGACCGATCTCCAGTT GGCAACCTGAACAAACACAA 094A2B03 AUBES4277 1632 386 28 80.6 TTGCTGGCTTTGCATCTGTA ATTGTGCATGGTTGCTCTGA 096A2H08 AUBES3493 537 321 28 81.4 TGGGTTTATGGCTGAAGACAC CTGCTCATGGTGCCGTTAAT 043A2D04 143 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES3007 622 541 28 82.5 TTAGCTGCTTCACCCTGGTC ATGGGTTTCCCCTAGTGCTC 080A2B05 AUBES1957 131 257 28 85.0 TTTGCATGGTACCTTCATGG CCCCTCGATCTATGCTCACT 022A2H04 AUBES1146 262 795 28 86.9 TCAAAACCAAAAGCAGGTCAGAC AGTGGCTGCTCTGAGGCGTG 007B1F03 AUBES1874 247 472 28 88.5 GGATGGGACGTCAATCCAT CCCACTGTTCAACAACATGC 026A1E04 AUBES2899 622 541 28 93.4 CATGTGCATACACACACTTTCTG TGACACAGCAGCACAAGTAGG 040A2E07 AUBES4767 2733 139 29 4.3 TGGAAGACCTGATTATTCTGTACG AACGGAAAGGAGCGATTTTAG 096A1H09 AUBES5244 2411 220 29 7.2 TGCGGTAGCACTGTAGATCG GCAGGTACCGCTGTAACGAG 079A1E04 AUBES4866 2174 262 29 7.6 CATCGCTGCCTATGGTGTC TGCATTACGTTTCCAAACCA 109A1A04 AUBES4797 2997 249 29 11.3 TCTTGCCTGCCCTTGTTTAC CGTACGTTGTTTTCTCCAAGG 051B1D06 AUBES3854 3420 260 29 29.6 TACAACTCGCTCACGCACTC AGTAAACGTGTCCCGGTTTG 050A2H04 AUBES4119 1558 424 29 34.6 TTCACAGGTGGGTGAAAACA TTTCTATCCGCCAGTTCCTC 059B1B10 AUBES4221 513 276 29 36.2 CAACCTACCGGGATTTCTGA CTCTTGGCCATCTTGGTGTT 077B2H05 AUBES3977 1769 161 29 36.2 TGATGGAGTTCACCAAGTTCC CCAGCCAATCAGATTCCACT 019A1A07 AUBES4691 2909 171 29 36.3 TGGGGGAATAAATGTGTTTTG GGCTTCGTGCATCATTAACC 076A1F05 AUBES5395 3457 276 29 39.2 GAACCCATCACTTTGTCACG TCTTATTTCCCCAAAATCAATCA 079A2A05 AUBES4696 2954 232 29 40.5 TCTCGACTTTCTCTGCCTGTC TTCCATCATCCTGCACTCTG 003B2A01 AUBES1955 singleton 29 42.2 CCACACATGCTTCCAATGAG GGAACTCGCTGAGCCTTTTT 021B1A04 AUBES4705 3088 365 29 45.7 CGTTTGCCAACAAACATCAC TCTCCAAAATTTGCAATCCA 052A1D09 AUBES4103 1099 406 29 52.8 GCAGAAGGAAACACCACCTC CCCCACGACCCTGTGTACTA 109A2A08 AUBES2189 1371 271 29 54.4 TGGCTGAATTGTCTGCCTAGT GGAATATTGTACGCTGCTTGC 027B1H08 AUBES3623 294 220 29 54.9 CCGATACCAGTCACAGAACCA CCTCACTCTTTCTCGGTGCT 009B1F10 AUBES3624 294 220 29 55.0 CCGATACCAGTCACAGAACCA CCTCACTCTTTCTCGGTGCT 026B1E01 AUBES4762 2704 173 29 55.9 ACAGCATCCGAATGTTCACC TGTGAATCCAGGGAGACTGA 057B1H03 AUBES3200 50 1049 29 56.6 AGGGTCCATCATGCTTTCTC GGGTAAAGTGCTATGCCTGTG 092B2B01 AUBES3289 324 320 29 56.6 TTTTGCATTGCTGGTCAGAG CGTCCAGTGTGAACAAGGTG 102B2G06 AUBES4484 1553 183 29 56.6 CTGGTTCAGCATGGATCAGT TTCACAGCCTTCTTTGCTCA 071B2B02 AUBES4337 2240 249 29 56.6 GACGGGCCGTTGAATTATTA ACTTATCGCACATGCTCTCG 077A1B07 AUBES1305 259 512 29 56.7 GTAGGCGGTCTGCCTCTCAG AACACAGCCTGCCTCTCATC 003A1D11 AUBES2699 259 512 29 57.5 TGCACCACTGGACTATGGAG CTGTAAGCTCACTGCCACCA 019B2H06 144 Marker Contig Contig length (Kbp) Linkage group Map position Forward primer Reverse primer BAC_ID AUBES2819 420 309 29 57.5 CCCTCTTCACTGCTGGTGTA TGTCATGCATATACTACTGCTACAAGA 031A1A08 AUBES3731 675 377 29 57.5 ACGTGTGCTGAGGGAGAGAC TTCTCACCAAATGCACCTACA 072B2D04 AUBES3554 261 174 29 57.6 GGTCTGTTTTTACTCTCGCTGAA TCTTCCTAAACCTCCTTCCTG 031B2F06 AUBES3478 261 174 29 58.0 GGTCTGTTTTTACTCTCGCTGA TGTCACCCAAGTCCATTAACA 078B1E09 AUBES5088 1503 166 29 58.1 GGCATTTCATTGAGGCAAA CAGTGGTGCTTTTGGAGTGA 106B1E04 AUBES3035 203 404 29 58.8 GACACGGGGAGTTCTGAAAC GTGCATTGTCTCATTCACAACA 058B1D07 AUBES2939 157 296 29 59.4 GCTGCCTGTTTGACCCTTAC TCGGTCATTACCTTGGCTGT 039A2F02 AUBES2740 60 688 29 59.7 GGAGTGTGGGCAGTAGATCG GAAAAGTGCACCGTCCTGTAA 057B2B03 AUBES3726 60 688 29 59.8 GCAGTAGATCGACAGGGAAAG AAAAGTGCACCGTCCTGTAA 053A1F05 AUBES3645 770 249 29 63.5 CCAACATGAAAACCTGTACCA GGCATAAAGGCTGGATTTACC 034B1D03 AUBES3644 770 249 29 63.6 CAGCTACTGCTTTGCTGCTG TTCGGCACGCTATACGAAG 056B2G04 AUBES2355 1920 281 29 77.8 AGGGTTCAAAAGTCCACTGC GCAATTCTCAAATGGGTAAATGA 047A2D10 AUBES2109 2022 208 29 80.1 GTGGCTCAGCTGGGTTTTT ACCACCAGCAACGTTAGACA 041B2B03 AUBES4583 1925 272 29 97.2 CAGCGTTAATGACGCAAGAA TCCAGTCCTCCGAGTCAAAC 080B1B10 AUBES5206 2194 316 29 97.2 GAACCGTCCTCTGCGTATGT CTGTTTGTGGGAATGCAGATT 054B1C04 145 Supplemental Table 2: Ratios of physical to genetic distance across all 29 LGs. Ratios were estimated based on the difference of the physical distances (Kb) and genetic distances (cM) between multiple BES markers mapped from the same contigs. LG Ctg Markers (AUBES) Kbp cM Kb/cM 1 1702 2148-2400 171 2.9 59 1 767 3565-3566 157 2.2 71 1 2142 3992-3993 259 2 130 1 2276 4540-4541 215 1.6 134 1 42 1924-2634-3108 961 6 160 1 597 1803-2358-2772 502 2.3 218 1 772 3567-3568 189 0.6 315 1 2439 1075-4414 347 1 347 1 316 3178-3209 573 1.3 441 1 949 3451-3452 162 0.2 810 1 468 3420-3421 179 0.1 1790 1 370 2627-3160 592 0.3 1973 1 2239 3745-3752 249 0.1 2490 1 1940 4860-4861 154 0 NA 1 1272 4436-4437 162 0 NA 1 351 3403-3404 216 0 NA 2 3007 1881-4698 176 8.4 21 2 343 2884-3952 166 7.4 22 2 145 2258-3394-3395 266 7.9 34 2 337 3625-3626 249 3.9 64 2 407 3627-3628 166 1.3 128 2 1706 4456-4457 232 1.7 136 2 1513 4162-4163 396 2.9 137 2 121 3184-3215 649 3.2 203 2 1693 1947-4170 247 1 247 2 1930 4848-4859 279 1 279 2 14 2676-3238 266 0.8 333 2 998 4434-4435 157 0.1 1570 2 570 1615-2085 178 0.1 1780 3 919 3771-3879-3880 223 6.8 33 3 377 3544-4350 228 5.3 43 3 477 1846-3491 259 4.4 59 3 335 2347-2595-3139 338 4.3 79 146 LG Ctg Markers (AUBES) Kbp cM Kb/cM 3 97 3074-4343 193 2.4 80 3 227 3065-3536 289 2.3 126 3 2932 4875-4876 164 0.7 234 3 480 2555-3327 201 0.7 287 3 789 3646-3647 216 0.7 309 3 702 3443-3444 203 0.4 508 3 1893 4578-4593 159 0 NA 4 703 2792-3312 161 2.7 60 4 442 2617-3154 440 2.2 200 4 623 2829-3306 242 0.7 346 4 676 3638-3639 303 0.8 379 4 94 2427-3012 127 0.2 635 4 787 1926-4083-4084 142 0.2 710 4 1487 3607-3608 159 0.2 795 4 3120 4708-4709 179 0.1 1790 4 1431 2505-4270 210 0.1 2100 4 2233 1555-4869 157 0 NA 4 557 4070-4071 200 0 NA 5 95 2982-3940 242 6.2 39 5 961 2182-4127 206 3.6 57 5 1110 1952-2993 284 4.1 69 5 2861 4685-4686 164 2 82 5 1002 4528-4529 205 1.8 114 5 341 2883-3002 490 2 245 5 2839 4425-4440-4441 183 0.7 261 5 1209 3594-3595 375 1.3 288 5 427 3629-3630 169 0.4 423 5 534 2478-2638-3170-3237 720 1.5 480 5 1044 3695-4244 151 0.2 755 5 853 3445-3446 369 0.4 923 5 190 2557-4346 173 0.1 1730 5 3037 2005-4700 304 0 NA 5 339 3402-4349 178 0 NA 5 1234 3596-4374 169 0 NA 6 1894 3701-3784 191 13.4 14 6 530 3004-3954-3955 174 4 44 6 974 3798-3799 220 4.8 46 6 93 2805-3280 142 2.7 53 6 346 2987-3255 296 4.7 63 6 786 3792-3793-3794 208 3 69 147 LG Ctg Markers (AUBES) Kbp cM Kb/cM 6 766 1306-2689 173 2.1 82 6 590 3433-3434-3664 223 2.7 83 6 1090 3591-4371 233 2.3 101 6 278 2602-3355-3384 208 1.8 116 6 776 2779-3690 184 1.5 123 6 1182 2195-4143-4144 301 2.1 143 6 229 3397-3398 230 1.6 144 6 2533 3844-3845 140 0.7 200 6 1098 2062-4003 228 0.9 253 6 193 3097-3155 247 0.8 309 6 37 1185-2623 225 0.7 321 6 1369 3777-4108 238 0.7 340 6 1058 3804-3805-3806 360 0.8 450 6 645 3789-3791 391 0.7 559 6 171 3514-3681 306 0.5 612 6 3026 4802-3852 191 0.3 637 6 196 2227-2811-3282 193 0.3 643 6 466 1935-2734-3016 445 0.5 890 6 29 2606-3359 595 0.3 1983 6 1031 3457-3458 159 0 NA 6 1455 3698-3892 151 0 NA 6 2630 3899-3900 203 0 NA 6 1635 3613-3614 377 0 NA 7 369 3408-3409 264 7.4 36 7 690 2318-2905 161 4.3 37 7 275 2562-3056 386 9.6 40 7 963 1809-4667-4668 191 3.5 55 7 9 2639-3703-3744 254 3.2 79 7 986 4134-4135 174 1.8 97 7 267 3399-3400-4348 208 1.9 109 7 608 2897-2898 337 2 169 7 1016 4097-4098 359 0.2 1795 7 328 1805-3718 318 0.1 3180 7 429 3487-3488 294 0 NA 7 216 2592-3136 196 0 NA 8 376 1786-2886-3256-3257 240 8.2 29 8 780 1283-1287-3569-4366 220 4.8 46 8 1907 4400-4401 257 5.5 47 8 159 3115-4345 213 4.2 51 8 314 1610-1635 352 5.9 60 148 LG Ctg Markers (AUBES) Kbp cM Kb/cM 8 2866 4426-4427-4442-4443 174 2.5 70 8 375 3747-3750 189 2.5 76 8 1208 2801-3321 127 1 127 8 189 3203-3326-3380 485 3.4 143 8 885 3194-3365 403 1.4 288 8 236 1763-2597-3717-3749 178 0.6 297 8 2128 1353-1990 149 0.5 298 8 455 3144-3357 127 0.2 635 8 388 3484-3485 201 0.3 670 8 1353 2802-3322 145 0.2 725 8 1909 1730-2057 201 0.1 2010 8 895 3573-3574-4367-4368 331 0.1 3310 8 443 3051-3324 394 0.1 3940 9 176 1843-2809-4659 161 14.3 11 9 72 2379-3199-3229-3230- 3653-3713-3716 1049 29.4 36 9 1684 3781-3818-3819-4280 233 2.4 97 9 154 3105-3162 397 1.9 209 9 1401 2103-2709-3720 401 1.3 308 9 490 2561-3055-3118 524 1.4 374 9 981 1761-3533 612 1.6 383 9 409 1410-4060 198 0.3 660 9 985 2051-3048 257 0.1 2570 10 89 2863-3939 252 4 63 10 111 3942-3943 166 2.5 66 10 559 2437-3302 167 2.4 70 10 1315 3600-3601 216 2.3 94 10 904 3317-3739 189 1.8 105 10 381 3412-3545 156 0.6 260 10 37 1867-3225 271 0.7 387 10 625 3500-3501 191 0.4 478 10 458 3631-3632 244 0.5 488 10 436 2580-3070 663 0.6 1105 11 793 3648-3649 228 20 11 11 360 2217-2844-4192 298 9.3 32 11 941 1765-3577 266 7 38 11 41 2859-2979 255 1.6 159 11 1178 4372-4373 139 0.8 174 11 20 1859-2601 413 2.3 180 11 225 3473-3474 184 0.8 230 149 LG Ctg Markers (AUBES) Kbp cM Kb/cM 11 161 2610-3090 435 1.6 272 11 646 2123-3527-3665 271 0.7 387 11 801 3867-3871-3872 205 0.5 410 11 1743 4293-4294 222 0.5 444 11 471 3424-3425 250 0.3 833 12 169 2050-3345 220 16.3 13 12 1552 4838-4839 245 7.3 34 12 609 1726-2579-3233 95 2.8 34 12 1800 3469-3470 235 4.8 49 12 441 3416-3546 281 4.5 62 12 596 3496-3497 232 3.6 64 12 828 4232-4233 137 1.8 76 12 1646 4383-4384 211 1.7 124 12 271 1888-3049 142 0.8 178 12 217 1725-3542 220 1.1 200 12 194 2075-3342 337 0.9 374 12 3196 4713-4714 299 0.2 1495 12 518 2420-2571-3124 331 0.2 1655 12 601 2773-3708 257 0.1 2570 12 897 4887-4888 203 0 NA 12 1073 4101-4138 137 0 NA 13 1131 4006-4007 223 6.2 36 13 738 3557-4362 216 5 43 13 929 1887-2422-2908 369 3.8 97 13 416 4516-4517 228 1.9 120 13 634 1896-2158 277 1.9 146 13 199 2609-3089-3702-3149 446 3 149 13 1246 4149-4150 318 1.5 212 13 401 3486-3555 222 0.9 247 13 59 2637-3110-3678-3734 448 1.6 280 13 36 2538-2678-2977 501 1.6 313 13 1221 2039-4017 225 0.7 321 13 652 3439-3440 323 0.8 404 13 92 2704-2864 218 0.5 436 13 700 2791-3311 211 0.1 2110 13 79 3392-3393 228 0 NA 14 156 2235-3615-3616 225 8.6 26 14 710 3640-4361 282 4.3 66 14 3090 4430-4431-4447 156 1.8 87 14 1324 2352-4154-4155 284 3.2 89 150 LG Ctg Markers (AUBES) Kbp cM Kb/cM 14 526 1922-2456-3426-3549 326 3.6 91 14 595 3435-3436 279 3 93 14 1989 4604-4605 174 1.7 102 14 704 1965-3957 166 1.6 104 14 1046 3584-3585 453 0.8 566 14 2508 4871-4872 291 0.5 582 14 175 3617-3618 222 0.2 1110 15 208 3619-3620 194 14.7 13 15 76 2861-3937-3938 117 4.8 24 15 717 2831-3314 225 6.3 36 15 588 2391-3431-3432 186 3.5 53 15 514 2891-2999 218 3.8 57 15 28 3506-3507 337 3.9 86 15 214 2581-3071-3131 399 3.6 111 15 323 1912-2881 299 2.5 120 15 818 3736-4124 157 1.2 131 15 34 3072-3728 144 1.1 131 15 671 2903-3265 139 0.7 199 15 958 1737-3040 296 1.3 228 15 104 1733-2622-3100-3158 624 1.7 367 15 116 2469-2599-3191-3354 413 0.8 516 15 363 1906-3407 286 0.1 2860 15 869 3873-3875 157 0 NA 16 435 3014-3723 181 16.2 11 16 1100 2679-2910 360 11.4 32 16 1062 3586-3587 303 3.7 82 16 38 1876-2594-2978-3348 700 7.8 90 16 101 2620-3196-3224-3368 518 3.8 136 16 1956 4628-4629 255 1.7 150 16 900 4432-4433 216 1.1 196 16 153 2387-3249 151 0.6 252 16 6 3190-3219-3353 291 1 291 16 241 2274-2611-3151-3362 649 2.1 309 16 644 1886-3637 215 0.1 2150 16 2920 4428-4429-4445 267 0 NA 17 987 3579-3580-4369 250 6.3 40 17 1509 3465-3466 374 3.5 107 17 1034 2267-2466-3583 269 2.5 108 17 454 3346-3347 200 1.8 111 17 604 3437-3551 181 0.8 226 151 LG Ctg Markers (AUBES) Kbp cM Kb/cM 17 2476 4415-4416 277 1.2 231 17 1988 4402-4403 189 0.6 315 17 790 2121-2605 435 1.3 335 17 885 2616-3221-3222 521 0.6 868 17 2065 2392-4323 161 0 NA 17 180 2873-3947 216 0 NA 17 386 4663-4664 152 0 NA 17 13 2604-3085 157 0 NA 17 139 2636-3168 494 0 NA 17 726 2121-2605 438 0 NA 17 1575 3609-4375 222 0 NA 18 632 1539-2900 129 5.9 22 18 168 1325-2650-4182-4183 367 13.1 28 18 222 2608-3088-3361 616 4 154 18 564 2826-3303 211 1.1 192 18 200 3161-3391 392 1.2 327 18 639 2341-2686 534 1.2 445 18 274 2501-3208 242 0.3 807 18 406 3414-3415 161 0 NA 18 918 3449-3552 267 0 NA 18 603 3263-3689 140 0 NA 19 876 2163-3770 331 1.5 221 19 181 1892-2612 240 1 240 19 141 1699-3755 198 0.7 283 19 1984 2354-4863 418 1.1 380 20 130 3133-3234-3382 242 13.8 18 20 481 1750-2889-2990-3746 266 4.5 59 20 1029 3801-3802-3909 286 3.4 84 20 1273 4020-4021 137 1.5 91 20 306 2596-3079 391 1.4 279 20 744 3270-3741 215 0.7 307 20 580 1338-3494 227 0.7 324 20 873 4236-4237 166 0.1 1660 20 263 4660-4661 186 0.1 1860 20 281 3227-3228-3390 966 0.2 4830 20 1067 2798-3320 198 0 NA 20 1502 2996-3009 232 0 NA 20 1490 3463-3464 164 0 NA 21 1001 4095-4096 179 4.3 42 21 103 1624-1945 428 8.9 48 152 LG Ctg Markers (AUBES) Kbp cM Kb/cM 21 2092 4404-4405 286 4.5 64 21 1214 1502-1629 176 2.1 84 21 455 2603-3084 173 1.7 102 21 212 2874-3948 225 1.7 132 21 418 1700-3295 194 1.4 139 21 209 2564-3336 396 2.4 165 21 182 3142-3218-3352 594 2.5 238 21 1695 3971-3972 240 1 240 21 183 2607-3087-3147-3360 622 2.3 270 21 242 2876-3001 389 0.6 648 21 742 3642-3643 184 0 NA 22 166 2231-2389-2635-3109-3167-3378 822 11.4 72 22 1017 3456-3553 193 2.4 80 22 973 1448-1941 233 2.7 86 22 1496 3814-3815 225 2.5 90 22 453 2237-2888-3719 330 3.5 94 22 358 2271-3405-3406 216 2.2 98 22 240 3518-3519 328 3.3 99 22 252 3163-3198 467 4.4 106 22 135 2593-3187 387 2.4 161 22 39 1968-3156 132 0.3 440 22 1341 4025-4026 240 0.1 2400 22 107 2565-3059 364 0.1 3640 22 1642 4844-4845 269 0 NA 23 2855 1411-4683 178 7.8 23 23 1351 1328-4031-4032 240 7.3 33 23 2114 1900-4327 120 2.5 48 23 382 2255-3046 313 4.3 73 23 74 2625-3102-3159 558 6.6 85 23 211 3188-3217-3657 539 4.3 125 23 1697 4289-4290 220 1.7 129 23 1040 4136-4137 362 1.8 201 23 1465 4115-4116 350 0.2 1750 23 982 2484-4132 218 0.1 2180 23 2434 1330-4412 156 0 NA 23 1229 4147-4148 232 0 NA 24 295 2490-3481 216 9.7 22 24 246 2563-3206-3335 279 3.7 75 24 2094 4864-4865 311 3.5 89 24 398 2681-3711 521 5.6 93 153 LG Ctg Markers (AUBES) Kbp cM Kb/cM 24 695 3502-3503-4360 426 3.2 133 24 838 2513-3693 169 1.1 154 24 890 2583-3073 502 3.2 157 24 823 2720-3041 252 0.1 2520 24 589 2788-3305 181 0 NA 25 81 4179-4180 223 8.7 26 25 1123 1857-1858-4141-4142 370 7.8 47 25 1851 1749-4284 134 1.5 89 25 678 2677-2904 186 1.6 116 25 599 1570-3032 250 1.6 156 25 177 1873-2624-3101 805 4.4 183 25 392 2621-3099-3157 839 4.2 200 25 1507 4450-4451 167 0.2 835 25 572 2450-3042 198 0.2 990 25 451 3419-3547 129 0.1 1290 25 1348 4028-4029 244 0.1 2440 25 144 2475-2869 247 0.1 2470 26 2357 1891-4410-4411 257 10.3 25 26 658 1872-2789 206 2.3 90 26 403 2547-2615-3095-3153 830 6.7 124 26 1621 4218-4220 254 1 254 26 142 2372-2698 184 0.7 263 26 40 1847-3091 232 0.6 387 26 1536 1964-4836-4837 387 0.9 430 26 1093 3592-3593 186 0.3 620 26 399 1333-4877 205 0.1 2050 27 463 1721-3260 208 9.4 22 27 1377 1951-4109 247 6.6 37 27 1551 1547-4378 176 4.2 42 27 9 2117-3231 419 9.9 42 27 1335 1747-5053 173 1.4 124 27 154 2629-3372 183 0.6 305 27 990 1714-1773-2124 284 0.6 473 27 1387 3021-3737 369 0 NA 27 844 3571-3572 161 0 NA 28 581 3430-3550-4355 220 62 4 28 762 3563-3564 200 5 40 28 622 2899-3007 482 10.9 44 28 5 2578-3068 308 5.8 53 28 147 2684-2870 335 5 67 154 LG Ctg Markers (AUBES) Kbp cM Kb/cM 28 262 1146-3179-3340-3341 521 6.8 77 28 138 4655-4656-4657 238 1.9 125 28 437 3686-4061-4062 164 1.2 137 28 318 1789-3288 169 1 169 28 582 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