|Vitamin B6 (pyridoxine) is a biological coenzyme
seemingly involved in environmental stress
tolerance and an important vitamin in human
nutrition produced in plants as well as fungi and
bacteria. A series of studies from these different
organisms revealed that SNZ1/SNO1 homologues are
the genes involved in the production of
pyridoxine. We decided to functionally define
SNO1 and SNZ1 orthologs and/or paralogs in the
Arabidopsis thaliana genome by using transgenic
yeast expressing putative SNZ1 or SNO1 homologs to
suppress the growth defects of yeast ?snz1 and
?sno1. Using a blast search we were able to find
Arabidopsis homologs of yeast SNZ1/SNO1.
Moreover, we have obtained knockout deletion
mutants of SNZ1 and SNO1 from S. cerevisiae and
were able to confirm that the SNZ1 and SNO1
deletions did not grow as efficiently as wild type
in media lacking pyridoxine. In addition, we were
able to clone the putative A. thaliana SNZ1 into a
high expression yeast shuttle vector (p426 GPD).
Our results indicate that the AtSNZ1 does
complement phenotypic expression of the SNZ1
knockout in yeast.