This Is AuburnElectronic Theses and Dissertations

LC-MS based metabolite fingerprinting and profiling of a traditional Chinese medicine YANG XIN® formulation




Almalki, Ahmad

Type of Degree

Master's Thesis


Pharmacal Sciences


For thousands of years, Traditional Chinese Medicine (TCM) has been used to treat or prevent various diseases, for this reason western countries have been attracted to and use TCM together with allopathic medicine. YANG XIN® is a TCM formulation used for nervous fatigue and consists of a 500 mg proprietary blend of concentrated extracts from 18 plants. In this study, liquid chromatography coupled to a quadruple time of flight (q-TOF) mass spectrometer was used for identification of constituents and quantification of chemical markers for quality control of the YANG XIN® formulation. Chapter One is an overview of TCM in general and YANG XIN® formulation in particular and its composition and usage. A brief review on the definition of chronic fatigue syndrome (CFS) and its symptoms are also included. Chapter Two talks about metabolite fingerprinting and profiling of YANG XIN® formulation by using LC-MS and NMR. It also describes the process from sample extraction to sample preparation and analysis, in addition to the identification of the analytical markers and the strategy used for selection of analytical markers in plant ingredients. Chapter Three describes the quantification process of YANG XIN® markers starting with sample preparation and ending with method validation.