This Is AuburnElectronic Theses and Dissertations

Neuroprotective effects of Resveratrol against oxidative stress and memory impairment in vivo and in vitro




Rege, Shraddha

Type of Degree



Nutrition and Food Science


Resveratrol is a polyphenolic phytoalexin known to exert anti-diabetic, anti-inflammatory and neuroprotective effects. Various studies have reported a link between obese diabetic state and Alzheimer’s disease. The present study evaluates the neuroprotective action of resveratrol against oxidative stress and memory associated proteins in obese (ob/ob) mice, and Amyloid beta (Aβ) treated H19-7 rat hippocampal cell line. Resveratrol was administered orally at the dose of 25 mg kg-1 body weight daily for 3 weeks to lean, obese (ob/ob) mice. Cultured rat hippocampal H19-7 neuronal cell line was pretreated with 75μM of resveratrol for 2 hrs followed by 25μM of Aβ (1-40) for 24 hrs. Resveratrol treatment did not alter body weight or blood glucose levels in ob/ob mice. The lipid peroxide levels were significantly increased in the brains of obese mice, and Aβ-treated H19-7 cells. The enzymic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and non-enzymic antioxidants like tocopherol, ascorbic acid and glutathione were decreased in obese mice brains and Aβ-treated H19-7 cells. Formic acid fractions in the brains of ob/ob mice, and Aβ-treated H19-7 cells were found to have increased expression of Tau, phosphorylated forms of tau (CP13, S202/205; PHF1, S396/404) and glial fibrillary acidic protein whereas decreased expression levels of Insulin Degrading Enzyme (IDE), phospho GSK 3β, synaptophysin, PSD-95, and ARC were observed as compared to the control group. Resveratrol treatment attenuated lipid peroxide levels, up-regulated the antioxidant activities, and increased the expression of proteins such as IDE, synaptophysin, PSD-95, ARC, and phospho GSK3β in both obese mice brains and Aβ- treated H19-7 cells. These findings suggest the neuroprotective effect of resveratrol in attenuating the oxidative damage and memory impairment in vivo and in vitro.