This Is AuburnElectronic Theses and Dissertations

Comparison of Plate Media for Isolation of Campylobacter from Live Broilers and Scheduled Delivery of Broiler Flocks to Reduce Cross-contamination with Campylobacter




Potturi-Venkata, Lakshmi-Pras

Type of Degree



Poultry Science


Campylobacters are bacteria that are a major cause of diarrheal illness in humans and are generally regarded as the most common bacterial cause of gastroenteritis worldwide. Disease control studies have demonstrated that 50 to 70% of human Campylobacter illness is attributed to consuming poultry and poultry products. Broiler chickens are frequently asymptomatic carriers of Campylobacter jejuni/coli and the organisms are common contaminants of processed broiler carcasses. Different selective media were originally designed to isolate C. jejuni from human feces. However, media for isolating campylobacters from broiler samples have not been fully evaluated. The objective of our first study was to compare the efficiency of different plate media for isolation of Campylobacter from broiler feces. Results from media comparison showed that mCCDA agar performed better followed by mCC, Bap-B, and Bap-H. Bap-B agar has performed similar to BAP-H agar, a version of BAP homemade in our laboratories using lysed horse blood instead of sheep blood. Campy-Line agar showed significantly lower counts when compared to other media. Colonization status of campylobacters drastically reduced on all sampling days in birds from gentamicin sulfate treated group when compared to the non-treated groups. PFGE profiles of isolates collected from different media plates showed similar pattern. The objective of our second study was to determine the effectiveness of logistic scheduling on the reduction of Campylobacter contamination. When, birds from positive farms with higher Campylobacter numbers were processed first followed by birds that have no/low contamination with Campylobacter, the numbers from the carcass rinse samples showed that the birds that are negative for Campylobacter at the farm, were positive from both pre-chill and post-chill samples. These results suggest cross-contamination of negative flocks during processing. On the contrary, negative birds processed earlier in the day, remained negative throughout processing and the positive birds that were processed following clean birds maintained a lower level of Campylobacter incidence. Isolates from different farms processed on the same day showed similar PFGE patterns, suggesting cross-contamination. This shows that Campylobacter-negative flocks can get contaminated with Campylobacter strains originating from the previously processed Campylobacter-positive flocks and that logistic scheduling may prevent cross-contamination