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An Investigation of Species Relationships within Marshallia Schreb. [Asteraceae] and Mitochondrial Genomes within Poaceae


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dc.contributor.advisorGoertzen, Leslie
dc.contributor.authorHall, Nathan
dc.date.accessioned2018-04-24T20:37:03Z
dc.date.available2018-04-24T20:37:03Z
dc.date.issued2018-04-24
dc.identifier.urihttp://hdl.handle.net/10415/6169
dc.description.abstractGenomic sequencing has become ubiquitous within the discipline of Biology. Here I use two case studies in which whole genomic DNA facilitates answers to foundational biological questions for organisms that do not have assembled genomes and have benefited little, if at all, from any sequencing in the past. Eleusine indica (L.) Gaertn. (Poaceae) is a prolific weed that is also a known genome donor for E. coracana (L.) Gaertn., a subsistence crop used in west Africa and east India. The genus Marshallia Schreb. (Asteraceae) comprises eight species, occurs throughout the southeast and is notable for inflorescences with a pink or white puffball appearance. In Chapter 1 general context and background is provided regarding both E. indica and Marshallia and the aims of the study are provided. Chapter 2 is an assembly of the mitochondrial genome of Eleusine indica and a survey of the mitochondrial gene content of Poaceae. Plant mitochondrial genomes are challenging to assemble because of large recombinogenic regions, this in turn makes surveys of mitochondrial gene content hard to accomplish without the use of extensive assembly efforts or laborious wet lab techniques. A new in silico approach is described and applied to determine mitochondrial gene content within publicly available datasets. The results are confirmed by past studies, but also expand sampling within Poaceae and provide new insight into the specific timing of mitochondrial gene loss in Poaceae. In Chapter 3 a repetitive element is assembled and characterized as a proof of concept for the usefulness of whole genomic low coverage sequence data within Marshallia. In Chapter 4 several facets of these data are employed to determine interspecies relationships within Marshallia. Seventeen datasets are used in phylogenetic analysis of assemble plastid genomes, entire ribosomal cistrons and mitochondrial sequences. Additionally the abundance of the repetitive element Marobo is characterized in Chapter 3 along with the relative abundance of several repetitive element superfamilies. Finally, a reduced set of transcriptomes is assembled and used for phylogenetic analysis. The results of all assemblies are compared and this yields the first in-depth exploration of the phylogenetic history of the species within Marshallia. Relationships within the Marshallia show species-specific divergence of the ribosomal DNA, which are in contrast to the interspecific sharing of cytotypes, this pattern is indicative of past introgression events.en_US
dc.rightsEMBARGO_NOT_AUBURNen_US
dc.subjectBiological Sciencesen_US
dc.titleAn Investigation of Species Relationships within Marshallia Schreb. [Asteraceae] and Mitochondrial Genomes within Poaceaeen_US
dc.typePhD Dissertationen_US
dc.embargo.lengthMONTHS_WITHHELD:61en_US
dc.embargo.statusEMBARGOEDen_US
dc.embargo.enddate2023-04-30en_US
dc.contributor.committeeRobert, Boyd
dc.contributor.committeeRashotte, Aaron
dc.contributor.committeeSantos, Scott

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