Comparison of the gene encoding the thyroid-stimulating hormone receptor between hyperthyroid and non-hyperthyroid domestic cats.
Metadata Field | Value | Language |
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dc.contributor.advisor | Behrend, Ellen | |
dc.contributor.author | Haysom, Laura | |
dc.date.accessioned | 2018-07-12T14:00:32Z | |
dc.date.available | 2018-07-12T14:00:32Z | |
dc.date.issued | 2018-07-12 | |
dc.identifier.uri | http://hdl.handle.net/10415/6278 | |
dc.description.abstract | The prevalence of feline hyperthyroidism (FH) is increasing, yet the etiology of FH remains to be elucidated. The purpose of this study was to compare exons 1 to 5 of the thyroid-stimulating hormone receptor (TSHR) gene and the predicted encoded amino acids between hyperthyroid and unaffected cats. Genomic DNA was isolated from whole blood of hyperthyroid and non-hyperthyroid cats. Exons 1 to 5 of the TSHR gene were amplified and sequenced. DNA and amino acid sequences were compared to identify differences between phenotypes. We identified 4 polymorphisms; 1 SNP in Exon 1 (Al-10-Val), 2 SNP in Exon 4 (silent mutations) and 1 SNP in Exon 5 (Al-139-Gly). In Exon 4, some cats had an intronic 30 base pair insertion and one hyperthyroid cat had a 2 base pair deletion upstream of the coding sequence. Finding a genetic basis for FH would have important implications for its treatment, screening and prevention. | en_US |
dc.rights | EMBARGO_NOT_AUBURN | en_US |
dc.subject | General Veterinary Medicine | en_US |
dc.title | Comparison of the gene encoding the thyroid-stimulating hormone receptor between hyperthyroid and non-hyperthyroid domestic cats. | en_US |
dc.type | Master's Thesis | en_US |
dc.embargo.length | MONTHS_WITHHELD:60 | en_US |
dc.embargo.status | EMBARGOED | en_US |
dc.embargo.enddate | 2023-07-03 | en_US |