Browsing by Author "Duin, Evert"

Now showing items 1-11 of 11

Activation and Inhibitor Studies on Methyl-Coenzyme M Reductase and Purification of a New Hydroxylamine Oxidoreductase from Methylomicrobium Album ATCC 33003

Yang, Na (2008-05-15)

Methyl-coenzyme M reductase (MCR) catalyzes the formation of methane from methyl-coenzyme M (CH3-S-CoM) and coenzyme B (HS-CoB) in methanogenic archaea. In order to manage the overproduction of methane in the environment, ...

Characterization of the iron center in cysteine dioxygenase and kinetic analyses of flavin binding by the alkanesulfonate flavin reductase

Sun, Honglei (2006-12-15)

Cysteine dioxygenase (CDO) and the flavin reductase, SsuE, are both important proteins in sulfur metabolism. CDO catalyzes cysteine oxidation which is a key step in cysteine metabolism in mammalian systems. SsuE is a flavin ...

Development and Synthesis of Mononuclear Superoxide Dismutase Mimics and Magnetic Resonance Imaging Contrast Agents

Hutchinson, Tessa (2021-04-26)

Over the past few years the Goldsmith group has reported mononuclear superoxide dismutase mimics and Mn(II) complexes with redox-active quinols that display rapid T1- weighted responses to H2O2. This dissertation will ...

Dual Mode Redox-Responsive MRI Contrast Agents

Polander, Hillary (2022-08-05)

Magnetic resonance imaging (MRI) contrast agents function by shortening the relaxation times of targeted nuclei, thereby better distinguishing regions with different local concentrations of the visualized nuclei. ...

Elucidating Interactions within Photoautotroph-Methanotroph Cocultures at Both Systems and Molecular Levels

Badr, Kiumars (2021-07-20)

In the US, the biogas produced from anaerobic digestion (AD) of industrial, municipal, and agricultural waste streams has a great potential as a renewable feedstock. To tap into the immense biogas potential while reducing ...

Functional Roles of Conserved Active Site Amino Acids in the Desulfonation Reaction Catalyzed by the Alkanesulfonate Monooxygenase from Escherichia coli

Carpenter, Russell (2008-12-15)

The flavin-dependent alkanesulfonate monooxygenase (SsuD) catalyzes the oxidation of 1-substituted alkanesulfonates producing sulfite and a corresponding aldehyde in the presence of O2 and FMNH2. The reduced flavin is ...

Isoprenoid synthesis: New roles for iron sulfur clusters

Adedeji, Dolapo (2007-08-15)

Spectroscopic investigations of the GcpE and LytB enzymes involved in the last two steps of the DOXP pathway for isoprene synthesis have been described. YfgB, one of the accessory proteins has also been characterized. ...

Methyl-coenzyme M reductase: Elucidating the process of activation and study of the effect of the methanogenesis inhibitor 3-nitrooxypropanol

Prakash, Divya (2014-12-17)

To use methane as a biocatalyst, it is important to establish a cell free system to convert the inactive form of methane forming enzyme i.e Methyl-coenzyme M reductase (MCR) to the active form. MCR catalyzes the reversible ...

Properties of Iron-Sulfur Clusters in Heterodisulfide Reductase

Engel, Carly (2019-07-17)

Global warming is a continuing crisis today. It has caused great damage to our planet and health. One of the leading causes of this issue originates from humans releasing an enormous amount of greenhouse gases into the ...

Roles of Two Interhelical Insertions in Catalase-peroxidase Catalysis: Tracing the Impact of Peripheral Protein Structures on Heme Enzyme Function

Li, Yongjiang (2005-08-15)

Monofunctional peroxidases and bifunctional catalase-peroxidases share almost superimposable active sites, yet peroxidases lack appreciable catalase activity. Moreover, catalase-peroxidases catalyze both catalase and ...

Structure, dynamics and interaction study of Glutaminase Interacting Protein (GIP) and its complex with Glutaminase L and Brain-specific Angiogenesis Inhibitor 2 (BAI2) peptide and characterization of subunit A of Heterodisulfide Reductase (HdrA) from Methanothermobacter marburgensis

Ovee, Mohiuddin (2013-07-30)

Glutaminase interacting protein (GIP) is a 124 amino acid long protein containing a single PDZ domain. This protein intersects a number of important biological pathways. In many of these pathways, the mechanism of function ...