Browsing by Author "Duin, Evert C."

Now showing items 1-9 of 9

Catalytic Mechanism of a Flavin-Dependent Alkanesulfonate Monoxygenase from Escherichia coli

Zhan, Xuanzhi (2008-08-15)

The flavin-dependent alkanesulfonate monooxygenase (SsuD) catalyzes the oxidation of alkanesulfonate to aldehyde and sulfite in the presence of O2 and FMNH2 provided by an FMN reductase (SsuE). The goal of these studies ...

Elucidating the flavin reductase mechanism in the alkanesulfonate monooxygenase system from Escherichia coli

Gao, Benlian (2006-12-15)

The two-component alkanesulfonate monooxygenase system from Escherichia coli is comprised of an FMN reductase (SsuE) and a monooxygenase enzyme (SsuD) that together catalyze the oxidation of alkanesulfonate to the corresponding ...

Micro-/Nanofabrication in Analytical Chemistry and Temperature Dependent Studies of Underpotential Deposition of Mercury(II) on Au(III)

Zhang, Lunsheng (2007-05-15)

This dissertation studies three routes to fabricate micro-/nano-sized structures for the purpose of analytical chemistry and the underpotential deposition (UPD) of mercury(II) onto Au(111) electrodes. In Chapter 2, we ...

Novel Nano-Liter Scale Microfluidic Platform for Protein Kinetics

Jambovane, Sachin (2008-12-15)

In the mid-1970s, integrated circuits exploited miniaturized transistors to bring a revolution in the field of computation. This marked the foundation of computers and information technology. In this century, we are on the ...

Probing the Reaction Mechanism of Methyl Coenzyme M Reductase

Wang, Mi (2008-08-15)

Methyl coenzyme M reductase (MCR) is the key enzyme in both microbial methane production and anaerobic methane oxidation. MCR catalyzes the reaction of methyl-coenzyme M (CH3SCoM) and coenzyme B (HSCoB) to methane and the ...

Role of Arabidopsis Thaliana Calmodulin Isoforms in Tolerance to Abiotic Stress

Al-quraan, Nisreen (2008-08-15)

Calmodulin and Ca2+ have been implicated in adaptation of plants to changes in its environment. Arabidopsis thaliana (Arabidopsis) genome has nine genetic loci encoding calmodulin protein isoforms. We have used wild type ...

Studies on The Synthesis of B-Ring 6-Azasteroids

Gandikota, Jyothi (2006-08-15)

Azasteroids, which carry a nitrogen in the C-6 position of the steroid nucleus are still mysterious and very few synthesized, were found to possess antimicrobial and antifungal properties apart from their pharmacodynamic ...

Studies to Elucidate the Mechanism of Reduced Flavin Transfer in the Alkanesulfonate Monooxygenase System from Escherichia coli

Abdurachim, Kholis (2007-12-15)

The two-component alkanesulfonate monooxygenase system utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation. The flavin reductase (SsuE) provides reduced ...

Two Iron-Sulfur Proteins Involved in Isoprenoid Biosynthesis with New Catalytic Roles

Xu, Weiya (2010-08-05)

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE or IspG) and (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB or IspH) are involved in the last two steps of the DOXP pathway for isoprenoid biosynthesis. ...