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Browsing by Author "Easley, Christopher"
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Automated microfluidic device development for metabolism, nutrient uptake, and hormone secretion analyses of primary endocrine tissues
Li, Xiangpeng (2017-07-25)
Pancreatic islets secret the dominant endocrine hormone, insulin, which controls the metabolic function fo nearly all other organ systems. Additionally, adipose tissue (fat) is now understood to be a complex, multicellular ...
Automated Microfluidic Droplet Generation and Merging with Integrated Salt-water Electrodes for Analyte Quantification and Monitoring of Endocrine Tissue Secretion Dynamics
Shi, Nan (2021-05-21) ETD File Embargoed
Adipose tissue, playing an important role in the global epidemic obesity and diabetes, has attracted extensive attention in recent decades. However, quantitative measurement of dynamics of nutrient uptake and metabolic ...
Customized Instrumentation for Small-Volume Electrochemistry and Microfluidics used in Bioanalytical Applications
Holtan, Mark (2018-11-20)
Disease biomarker detection allows diagnosis of both chronic and acute conditions. Although current detection methods are adequate for several targets (e.g. enzymatic biosensors), the stability and limited number of targets ...
The Development of a Nucleic Acid-Directed Switch and Proximity Dependent Protein Assays using Electrochemical Techniques
Ford, Katarena (2018-10-09)
The analysis of nucleic acids (NA), has increased demand for point-of-care systems, such as rapid preventive health care, forensic applications, etc. Thus inspired, a vast amount of biosensor platforms for the detection ...
Development of Analytical Systems for Detection of Biomarkers and Neurotoxic Exposure
Zhou, Lang (2019-04-23)
Exposure to the chemical and biological compounds in environment poses a serious health threat to humanity. Timely awareness of the chemicals in environment plays a vital role in exposure assessment, damage control, and ...
Development of Oligonucleotide, Small Molecule, and Protein Assays using Square-wave Voltammetry with Electrode-bound Nucleic Acid Nanostructures
Somasundaram, Subramaniam (2018-01-09)
Biomarker analysis plays a decisive role in health care. This role is not only limited to disease diagnosis, but it also involves understanding of biological and physiological functions in order to monitor health, observe ...
Development of real-time, quantitative free fatty acid uptake assays for adipose tissue explants using fluorescence and microfluidics
Hagos, Tesfagebriel (2017-04-25)
Free fatty acid (FFA) uptake by adipocytes in response to nutritional or hormonal stimuli is a key process in the complex metabolic cascade leading to diabetes and obesity. The work presented in this thesis is focused on ...
Development of thermally regulated homogeneous assays and droplet-based microfluidic tools for probing secretion dynamics from pancreatic and adipose tissues
Hu, Juan (2018-07-24)
Cells can transmit signals and adapt to microenvironment variations rapidly with dynamic processes, such as biomolecule secretion for signal delivery. Dynamic, oscillatory secretion is expected to exist in many cell ...
Exploring actinide chemistry: uranyl detection and thorium catalysis
Tutson, Charmaine (2017-04-21)
In a world where there is a strong need to improve our means of producing energy to sustain our ever expanding population with low green-house emissions, nuclear power offers sustainability and low greenhouse emissions, ...
Formation and Passivation of Sub-Nanoliter Droplets for High Throughput Biological Assay Platforms
DeJournette, Cheryl (2014-07-24)
In Vitro Compartmentalization (IVC) is a technique that utilizes water-in-oil emulsion droplets of fL – nL volume to compartmentalize and perform biological reactions and assays in parallel. At its highest capacity it has ...
Giant Janus Liposomes: Preparation, Self-Assembly and Motion
Liu, Zening (2020-07-17)
Presented in this Dissertation are a series of studies on Janus liposomes, their formation, self-assembly and motion behaviors. Liposomes are closed, spherical lipid bilayer assemblies dispersed in water. Since their ...
How an Arginine Switch Promotes the Self-preservation of an Hydrogen Peroxide-degrading Enzyme KatG: the Strategic Use of an Active-Site Tryptophan
Xu, Hui (2020-07-30)
Catalase-peroxidase (KatG) is a heme-dependent enzyme and an effective H2O2 disposal catalyst, sustaining life for many archaea, bacteria, and lower eukaryotes. Many pathogens employ KatG catalase activity (2 H2O2 -> 2 H2O ...
Identification of Pesticides Using Experimental and Computational Approaches based on Ion Mobility Mass Spectrometry Measurements
Benedicta, Donkor (2022-07-14)
Mass spectrometry is known to be a powerful analytical technique due its high sensitivity, specificity, speed, and versatility. It has the capability of detecting and identifying numerous analytes in sample matrices. In ...
Improved Proximity Assays for Protein Quantitation
Hu, Jiaming (2013-06-05)
The work presented in this dissertation is focused on the development of proximity assay formats for biosensor applications in medical diagnostics, biological research, and point-of-care testing. An electrochemical proximity ...
KATG as a Defense Against Hydrogen Peroxide Toxicity: From a Redundant C-Terminal Domain to the Paradoxical Synergy of Two Mutually Antagonistic Activities
Duan, Haijun (2014-12-09)
Catalase-peroxidases (KatGs), first discovered in 1979, are heme-dependent enzymes mainly found in bacteria, archaea and fungi. They are capable of decomposing H2O2 by catalatic and peroxidatic pathways. KatG has garnered ...
Lock-In Detection with Microfluidic Droplets for Quantitative Bioanalysis in Sub-Nanoliter Volumes
Deal, Kennon (2013-11-15)
The following chapters introduce and describe novel droplet microfluidic techniques to assist with limits of detection of analytes with low signal-to-noise ratios. The approach is proven feasible with absorbance measurements ...
Metal Complexation and Extraction Studies of bis-dithiophosphinite Ligands
DeVore, Michael, II (2011-12-06)
Five bis(dithiophosphonite) ligands OR1a-OR3b were synthesized using Lawesson’s Reagent and analogs of Lawesson’s Reagent. Two of the ligands OR2a and OR3a, were used in extraction studies with uranyl nitrate. UV-Vis ...
Microfluidic Circuit Designs for Nanoliter-Scale Flow Control and Highly Sensitive Quantitative Bioanalysis
Negou Negou, Jean (2017-12-04)
Within this dissertation, we have developed several new microfluidic circuit components that permit precise flow control at the nanoliter scale, and we have demonstrated their successful applications in either proof-of-concept ...
Microfluidic Interfacing for Primary Endocrine Tissue: Developing Bioanalytical Methodologies and Novel Fabrication Methods for Cell Culture and Analysis
Brooks, Jessica (2016-12-09)
In order to bridge the gap between microfluidic designs and real world applications, microanalysis systems were developed using minimalistic design attributes which could be readily paired with compatible bioanalytical ...
Passive microfluidic methods for sampling hormone secretions from primary islets and adipocytes
Godwin, Leah (2013-07-11)
We have developed an 8-channel passive microfluidic device that is capable of sampling both primary islet and adipocytes. Although there are microdevices already published in the literature; they require bulky external ...