Browsing by Author "Mansoorabadi, Steven"

Now showing items 1-14 of 14

Catalase-Peroxidase: A Structure that Facilitates Electron Transfer, Protein-based Cofactor Formation, and Antibiotic Activation

Barton, Callie (2022-12-09)

Catalase-peroxidase (KatG) is a heme-dependent enzyme that uses a single active for the degradation of H2O2 by two distinct mechanisms. One is a catalase mechanism where two equivalents of H2O2 are disproportionated with ...

Computational Studies of Dinoflagellate Luciferase and Radical S-adenosyl-L-methionine Enzymes

Donnan, Patrick Hugh (2022-12-06)

This dissertation seeks to apply computational methods to study two enzymatic systems: dinoflagellate luciferase and radical S-adenosyl-L-methionine enzymes. Dinoflagellates are microorganisms, many of which are capable ...

Development of thermally regulated homogeneous assays and droplet-based microfluidic tools for probing secretion dynamics from pancreatic and adipose tissues

Hu, Juan (2018-07-24)

Cells can transmit signals and adapt to microenvironment variations rapidly with dynamic processes, such as biomolecule secretion for signal delivery. Dynamic, oscillatory secretion is expected to exist in many cell ...

Functional Genomics Investigation of Siroheme and Heme d1 Biosynthesis

Owens, Victoria (2019-06-12)

Many bacteria and archaea grow under anaerobic or microaerophilic conditions. In order for these organisms to survive under these conditions, many utilize nitrate or nitrite as terminal electron acceptors for anaerobic ...

Giant Janus Liposomes: Preparation, Self-Assembly and Motion

Liu, Zening (2020-07-17)

Presented in this Dissertation are a series of studies on Janus liposomes, their formation, self-assembly and motion behaviors. Liposomes are closed, spherical lipid bilayer assemblies dispersed in water. Since their ...

How an Arginine Switch Promotes the Self-preservation of an Hydrogen Peroxide-degrading Enzyme KatG: the Strategic Use of an Active-Site Tryptophan

Xu, Hui (2020-07-30)

Catalase-peroxidase (KatG) is a heme-dependent enzyme and an effective H2O2 disposal catalyst, sustaining life for many archaea, bacteria, and lower eukaryotes. Many pathogens employ KatG catalase activity (2 H2O2 -> 2 H2O ...

Identification of Pesticides Using Experimental and Computational Approaches based on Ion Mobility Mass Spectrometry Measurements

Benedicta, Donkor (2022-07-14)

Mass spectrometry is known to be a powerful analytical technique due its high sensitivity, specificity, speed, and versatility. It has the capability of detecting and identifying numerous analytes in sample matrices. In ...

The Mechanistic Studies of Dinoflagellate Bioluminescence and the Conformational Analysis of Dinoflagellate Luciferase

Nguyen, Kenny (2023-05-10) ETD File Embargoed

This dissertation discusses the luciferase enzyme of dinoflagellates, which are eukaryotic microorganisms found in both freshwater and marine environments. Dinoflagellates are known for their capability for bioluminescence. ...

Methyl-coenzyme M reductase: Expressing active recombinant enzyme in M. maripaludis, and investigating the activation process, and the mechanism of inhibition of 3-nitrooxypropanol and Studying the effect of Ionic liquids on the mononuclear rearrangement of heterocycles using QM/MM method

Ghebreab, Robel (2019-07-25)

The study of the biosynthesis of methane is appealing from both its potential role in biofuel production and environmental perspectives. Methane is the main component of natural gas, and it is also a potent greenhouse gas ...

Novel Enzymes and Catalytic Mechanisms in Tetrapyrrole Metabolism: a Nitrogenase Homolog and a Noncanonical Heme Oxygenase

Li, Shuxin (2021-07-20)

Methane is the major component of natural gas, which accounts for approximately one quarter of the world’s energy demands. There are many advantages to using methane as a fuel. Methane is an abundance resource. A huge ...

Rapid Detection and Accurate Discrimination of Microorganisms by Liquid Chromatography, High-Resolution Ion Mobility, and Tandem Mass Spectrometry

Olajide, Orobola (2024-04-22)

Determining bacterial identity at the strain level is crucial for public health to enable appropriate medical treatment and reduce hospitalization times and antibiotic resistance. To achieve this goal, we have reported the ...

A Story About Light and Energy: Mechanistic and Biosynthetic Investigations of Bioluminescence and Methanogenesis

Ngo, Phong (2016-12-12)

This dissertation seeks to investigate underexplored aspects of dinoflagellate bioluminescence and biological methane production. Ubiquitous in the four oceans, dinoflagellate bioluminescence is a consequence of an ...

A Study of Methyl-coenzyme M Reductase Maturation: Coenzyme F430 Biosynthesis and Post-translational Modifications

Zheng, Kaiyuan (2018-10-03)

This dissertation seeks to investigate the coenzyme F430 biosynthetic pathway and post-translational modification (PTM) enzymes of methyl-coenzyme M reductase (MCR), which will have applications in natural gas-to-liquid ...

Transition from classical methods to new strategies: Mechanistic evaluation of inhibitors against Mycobacterium tuberculosis shikimate kinase

René Fuanta, Ngolui (2018-07-24)

Tuberculosis (TB) still stands as one of the leading causes of mortality resulting from an infectious agent. In part, this stems from the emergence of multidrug and extensive drug-resistant strains of tubercle bacilli, ...