Browsing Auburn Theses and Dissertations by Author "Goodwin, Douglas"

Now showing items 21-36 of 36

Mechanisms Underlying Myeloperoxidase Inhibition: Evaluating the Efficacy and Safety of Anti-inflammatory MPO Inhibitors Designed to Limit the Generation of Reactive Oxygen and Nitrogen Species

Huang, Jiansheng (2015-07-24)

Myeloperoxidase (MPO), a heme-containing peroxidase, plays a critical role in the innate immune response by producing hypochlorous acid. MPO-mediated oxidation is also associated with chronic obstructive pulmonary disease ...

Methyl-coenzyme M reductase: Elucidating the process of activation and study of the effect of the methanogenesis inhibitor 3-nitrooxypropanol

Prakash, Divya (2014-12-17)

To use methane as a biocatalyst, it is important to establish a cell free system to convert the inactive form of methane forming enzyme i.e Methyl-coenzyme M reductase (MCR) to the active form. MCR catalyzes the reversible ...

Probing the Reaction Mechanism of Methyl Coenzyme M Reductase

Wang, Mi (2008-08-15)

Methyl coenzyme M reductase (MCR) is the key enzyme in both microbial methane production and anaerobic methane oxidation. MCR catalyzes the reaction of methyl-coenzyme M (CH3SCoM) and coenzyme B (HSCoB) to methane and the ...

Resolving the Paradoxical Nature of a Bifunctional Enzyme: Pathways and Regulation of Intramolecular Electron Transfer In KatG

Njuma, Olive (2016-08-05)

Catalase-peroxidases (KatGs) are heme-dependent enzymes that use a single active site to perform two activities by protein-based radical dependent mechanisms. As a catalase, in its first step, it reduces H2O2 to water and ...

Revalidating DNA Damage Inducible 1 Homolog II (DDI2) as a Novel Target for the Treatment of Cancer

Ibtisam, Ibtisam (2023-05-04)

Cancer cells are under proteotoxic stress and are more sensitive to the perturbation of protein quality control pathways than normal cells. The proteasome is a critical component of the protein quality control machinery. ...

Role of Distant, Intrasubunit Residues in Catalase-Peroxidase Catalysis: Tracing the Role of Gene Duplication and Fusion in Enzyme Structure and Function

Cook, Carma (2009-05-08)

In spite of being positioned 30 Å away from the active site, the C-terminal domain is essential for catalase-peroxidase (KatG) function. Addition of equimolar amounts of separately expressed and isolated C-terminal domain ...

Roles of an 'Inactive' Domain in Catalase-Peroxidase Catalysis: Modulation of Active Site Architecture and Function by Gene Duplication

Hartfield-Baker, Ruletna (2006-12-15)

Within biological systems iron is a transition metal that allows access to the benefits of molecular oxygen as an oxidant. However, with these benefits come grave consequences if the reactions are not strictly controlled. ...

Roles of Large Loops in Catalytic Versatility of Catalase-Peroxidases: Significance of Peripheral Structures in Improvising Enzyme Functions

Kudalkar, Shalley (2012-05-07)

Catalase-peroxidases are bifunctional enzymes that catalyze the removal of hydrogen peroxide by two distinct pathways (catalase and peroxidase). They are central to antibiotic resistance in Mycobacterium tuberculosis and ...

Seeking MRNA Methylation Inhibitors as Antiviral Agents

Li, Weikuan (2008-08-15)

Aristeromycin and neplanocin A are two examples of potent S-adenosylhomocysteine hydrolase inhibitors. As a consequence, they show significant broad-spectrum antiviral activity, however, their clinical potential is limited ...

Studies to Elucidate the Mechanism of Reduced Flavin Transfer in the Alkanesulfonate Monooxygenase System from Escherichia coli

Abdurachim, Kholis (2007-12-15)

The two-component alkanesulfonate monooxygenase system utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation. The flavin reductase (SsuE) provides reduced ...

A Study of Methyl-coenzyme M Reductase Maturation: Coenzyme F430 Biosynthesis and Post-translational Modifications

Zheng, Kaiyuan (2018-10-03)

This dissertation seeks to investigate the coenzyme F430 biosynthetic pathway and post-translational modification (PTM) enzymes of methyl-coenzyme M reductase (MCR), which will have applications in natural gas-to-liquid ...

Synergy not Antagonism in Antioxidant Defenses: The Unanticipated Effect of Electron Donors on Catalase-peroxidase Function

Ndontsa, Elizabeth (2013-04-22)

Catalase-peroxidases, also called KatGs, have raised considerable interest due to their role in the activation of isoniazid, an anti-tubercular pro-drug for Mycobacterium tuberculosis. KatGs are heme-dependent enzymes and ...

Systemic Inflammatory Response To Consecutive Days of Exercise In The Heat

Barberio, Matthew (2013-04-23)

PURPOSE: The purpose of this study was to determine the LPS-mediated SIR to acute bouts of exercise in the heat and any adaptive response that occurs during consecutive days of exercise in the heat. METHODS: 8 healthy males ...

Towards the Understanding of Complex Biochemical Systems: the Significance of Global Protein Structure and Thorough Parametric Analysis

Moore, Robert (2009-07-06)

Enzymes are a highly diverse set of macromolecules, and, given their size and biocatalytic significance, each is an extremely complex system to study. As such, many assumptions must be made to simplify these systems to a ...

Transition from classical methods to new strategies: Mechanistic evaluation of inhibitors against Mycobacterium tuberculosis shikimate kinase

René Fuanta, Ngolui (2018-07-24)

Tuberculosis (TB) still stands as one of the leading causes of mortality resulting from an infectious agent. In part, this stems from the emergence of multidrug and extensive drug-resistant strains of tubercle bacilli, ...

VO2 on-kinetics in isolated canine muscle in situ during slowed convective O2 delivery

Goodwin, Matthew (2008-12-15)

A substantial body of evidence now suggests that while increasing O2 delivery to a working muscle during submaximal contractions onset may not speed VO2 on-kinetics, slowing the rate of O2 delivery may slow the VO2 on-kinetics ...