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Genome Wide Association Study (GWAS) on Root-Knot Nematode Resistance in Cultivated Peanut


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dc.contributor.advisorChen, Charles
dc.contributor.authorKumral, Fulya Eda
dc.date.accessioned2019-07-16T16:27:53Z
dc.date.available2019-07-16T16:27:53Z
dc.date.issued2019-07-16
dc.identifier.urihttp://hdl.handle.net/10415/6824
dc.description.abstractThe peanut root-knot nematode, Meloidogyne arenaria, is one of the major soil-borne pests for peanut (Arachis hypogaea L.). It causes economic losses in the production of peanut in the southeastern region, especially in Alabama, Georgia, Florida, and in Texas as well. Losses due to root-knot nematodes can reach up to 50% at dense infested fields without using nematicides. The use of nematode resistant cultivars is the most convenient economical way of biological control method for producers. The identification of resistant peanut germplasm to nematode diseases is a fundamental task for breeding nematode resistant cultivar. The objectives of this research are to evaluate 161 accessions of peanut germplasm in the greenhouse for resistance and to identify SNP markers associated with root-knot nematode resistance via genome-wide association study (GWAS). Randomized complete block design with three replications for each genotype is performed for phenotyping by using greenhouse inoculation techniques. The genetic diversity panel used in this experiment was genotyped by Affymetrix version 2.0 SNP assay. Forty-six quantitative trait loci (QTLs) located on twelve different chromosomes underlying root-knot nematode resistance were determined with phenotypic variation explained (PVE) between 7.8% and 17% by GWAS. Out of 46 QTLs, 957 candidate genes detected including 520 genes on A sub-genome and 437 genes o on B sub-genome. Specifically, 26 candidate genes related to LRR encoding gene were found on chromosomes A01, A04, A05, B07, B08, and B10. The associated markers could be applied in breeding programs for marker assisted selection.en_US
dc.rightsEMBARGO_NOT_AUBURNen_US
dc.subjectCrop Soils and Environmental Sciencesen_US
dc.titleGenome Wide Association Study (GWAS) on Root-Knot Nematode Resistance in Cultivated Peanuten_US
dc.typeMaster's Thesisen_US
dc.embargo.lengthMONTHS_WITHHELD:12en_US
dc.embargo.statusEMBARGOEDen_US
dc.embargo.enddate2020-07-16en_US
dc.contributor.committeeLawrence, Kathy
dc.contributor.committeeSanz-Saez, Alvaro

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