Browsing Auburn Theses and Dissertations by Author "Goodwin, Douglas"

Now showing items 1-20 of 36

Alkaline Pretreatment of Biomass for Ethanol Production and Understanding the Factors Influencing the Cellulose Hydrolysis

Gupta, Rajesh (2008-05-15)

Alkaline pretreatments were investigated in connection with bioconversion of lignocellulosic biomass into ethanol. Corn stover and two different batches of hybrid poplar (High Lignin (HL) and Low Lignin (LL)) were the ...

Biohybrid and Biomimetic Platforms for Programmable Therapeutic Delivery

Venkatesh, Siddarth (2008-08-15)

Drug and gene delivery carriers such as hydrogels and metallic nanoparticles have shown remarkable promise as programmable platforms in the targeted delivery of their payload. In this work, the fundamental principles of ...

Catalase-Peroxidase: A Structure that Facilitates Electron Transfer, Protein-based Cofactor Formation, and Antibiotic Activation

Barton, Callie (2022-12-09)

Catalase-peroxidase (KatG) is a heme-dependent enzyme that uses a single active for the degradation of H2O2 by two distinct mechanisms. One is a catalase mechanism where two equivalents of H2O2 are disproportionated with ...

Catalytic Mechanism of a Flavin-Dependent Alkanesulfonate Monoxygenase from Escherichia coli

Zhan, Xuanzhi (2008-08-15)

The flavin-dependent alkanesulfonate monooxygenase (SsuD) catalyzes the oxidation of alkanesulfonate to aldehyde and sulfite in the presence of O2 and FMNH2 provided by an FMN reductase (SsuE). The goal of these studies ...

Characterization of the iron center in cysteine dioxygenase and kinetic analyses of flavin binding by the alkanesulfonate flavin reductase

Sun, Honglei (2006-12-15)

Cysteine dioxygenase (CDO) and the flavin reductase, SsuE, are both important proteins in sulfur metabolism. CDO catalyzes cysteine oxidation which is a key step in cysteine metabolism in mammalian systems. SsuE is a flavin ...

Comparative Analyses and Structural Modeling of RNAs Participating in Non-Canonical Initiation of Protein Synthesis

Burks, Jody (2010-12-09)

Transfer-messenger RNA and the Internal Ribosomal Entry Site (IRES) RNAs of Foot and Mouth Disease Virus (FMDV) and Bovine Viral Diarrhea Virus (BVDV) play important roles in trans-translation and cap-independent protein ...

Defining the Porcine Colostral Proteome: Changes in the Array of Proteins from Colostrum to Mature Milk

Silva, Alejandro (2011-08-01)

The importance of colostrum (first milk) for immunological and nutritional support of newborn mammals is well established. Many bioactive peptides are present in milk at higher concentrations than in maternal circulation. ...

Development of thermally regulated homogeneous assays and droplet-based microfluidic tools for probing secretion dynamics from pancreatic and adipose tissues

Hu, Juan (2018-07-24)

Cells can transmit signals and adapt to microenvironment variations rapidly with dynamic processes, such as biomolecule secretion for signal delivery. Dynamic, oscillatory secretion is expected to exist in many cell ...

The Effect of Pharmacological and Dietary Modulators of Lipid Metabolism on Gene Expresion in a Porcine Model

Tang, Ling (2006-12-15)

Modifying the size and/or carcass distribution of adipose tissue in meat producing animals by nutritional and pharmacological means has long been of interest to animal scientists. In pigs, as in other meat animals, a more ...

Elucidating the flavin reductase mechanism in the alkanesulfonate monooxygenase system from Escherichia coli

Gao, Benlian (2006-12-15)

The two-component alkanesulfonate monooxygenase system from Escherichia coli is comprised of an FMN reductase (SsuE) and a monooxygenase enzyme (SsuD) that together catalyze the oxidation of alkanesulfonate to the corresponding ...

Enhancing Expression of Recombinant Hemoproteins: Progress Toward Understanding Structure/Function and Therapeutic Application

Varnado, Cornelius (2006-08-15)

Nature has developed ways to control the essential need to activate oxygen. Hemoproteins perform this task using iron in a prosthetic group (e.g., iron-protoporphyrin-IX or heme). The environment surrounding the heme iron ...

Evaluation of Bacillus biocontrol potential through the lens of secondary metabolite diversity in genomic, enzymatic, and chemical space

Alam, Md Jahangir (2022-08-04)

Global food security is under threat by plant-pathogenic oomycetes and fungi. The overuse and misuse of pesticides against such pathogens triggers fungicide resistance and long-term environmental contamination. This warrants ...

Functional Roles of Conserved Active Site Amino Acids in the Desulfonation Reaction Catalyzed by the Alkanesulfonate Monooxygenase from Escherichia coli

Carpenter, Russell (2008-12-15)

The flavin-dependent alkanesulfonate monooxygenase (SsuD) catalyzes the oxidation of 1-substituted alkanesulfonates producing sulfite and a corresponding aldehyde in the presence of O2 and FMNH2. The reduced flavin is ...

Gene Duplication and Fusion: Strategy for Active Site Control and Starting Point for New Catalysts

Wang, Yu (2012-12-10)

Catalase–peroxidases (KatGs) have two peroxidase-like domains. The N-terminal domain contains the heme-dependent, bifunctional active site. Though the C-terminal domain lacks the ability to bind heme or directly catalyze ...

How an Arginine Switch Promotes the Self-preservation of an Hydrogen Peroxide-degrading Enzyme KatG: the Strategic Use of an Active-Site Tryptophan

Xu, Hui (2020-07-30)

Catalase-peroxidase (KatG) is a heme-dependent enzyme and an effective H2O2 disposal catalyst, sustaining life for many archaea, bacteria, and lower eukaryotes. Many pathogens employ KatG catalase activity (2 H2O2 -> 2 H2O ...

The Identification of Critical Active-Site Groups and Catalytic Steps Governing the Desulfonation Reaction by Alkanesulfonate Monooxygenase

Robbins, John (2012-10-08)

The alkanesulfonate monooxygenase enzyme (SsuD) catalyzes the oxygenolytic cleavage of a carbon-sulfur bond of sulfonated substrates. A mechanism involving acid-base catalysis is proposed for the desulfonation mechanism ...

Improving bipolar electrode sensors by improving electrochemiluminescence efficiency of Ru(bpy)3Cl2

Khan, Waliul (2020-09-28)

Bipolar electrochemistry belongs to the branch of electrochemistry where cathodic and anodic processes can be coupled. As there is no direct contact establishes between the bipolar electrodes and electrical connection, the ...

Investigating a novel protein-based cofactor: toward elucidating the catalase mechanism of Mycobacterium tuberculosis KatG

Aziz, Tarfi (2022-12-07)

Catalase-peroxidases (KatGs) have been engineered by nature to exhibit dual functionalism, degrading H2O2 by catalase and peroxidase mechanisms. This serves to protect the organisms that carry it (primarily bacteria and ...

Iron-Sulfur Cluster-Containing Enzymes Involved in Isoprenoid Biosynthesis and Electron Bifurcation

Ghebreamlak, Selamawit (2016-05-05)

Iron-sulfur-cluster-containing proteins are present in all living organisms. They are involved in a wide array of biological processes such as electron transfer, substrate binding and activation, regulation of enzyme ...

Isoprenoid synthesis: New roles for iron sulfur clusters

Adedeji, Dolapo (2007-08-15)

Spectroscopic investigations of the GcpE and LytB enzymes involved in the last two steps of the DOXP pathway for isoprene synthesis have been described. YfgB, one of the accessory proteins has also been characterized. ...